Desulfurobacterium indicum
Average proteome isoelectric point is 7.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1617 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R1MJD2|A0A1R1MJD2_9BACT CitMHS domain-containing protein OS=Desulfurobacterium indicum OX=1914305 GN=BLW93_08200 PE=4 SV=1
MM1 pKa = 7.5 WLVDD5 pKa = 3.91 LSGSFGDD12 pKa = 6.48 DD13 pKa = 3.22 LFTWQNNTEE22 pKa = 4.42 AIVNALKK29 pKa = 10.08 TKK31 pKa = 10.5 ISDD34 pKa = 3.64 LRR36 pKa = 11.84 VGLASFVDD44 pKa = 4.78 APCSGFGYY52 pKa = 10.69 DD53 pKa = 4.03 GDD55 pKa = 3.9 YY56 pKa = 11.02 GYY58 pKa = 11.25 KK59 pKa = 10.43 LEE61 pKa = 5.06 LGCVNNFVV69 pKa = 3.77
Molecular weight: 7.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 4.012
IPC_protein 3.897
Toseland 3.681
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.91
Rodwell 3.732
Grimsley 3.605
Solomon 3.897
Lehninger 3.846
Nozaki 4.062
DTASelect 4.317
Thurlkill 3.783
EMBOSS 3.91
Sillero 4.024
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.926
Protein with the highest isoelectric point:
>tr|A0A1R1MMS6|A0A1R1MMS6_9BACT Ferredoxin-NADP reductase OS=Desulfurobacterium indicum OX=1914305 GN=BLW93_01710 PE=4 SV=1
MM1 pKa = 6.77 YY2 pKa = 10.36 KK3 pKa = 10.17 KK4 pKa = 10.43 VFSRR8 pKa = 11.84 LFVAGILGMLVFLLWRR24 pKa = 11.84 GINFTNAASFIYY36 pKa = 9.92 GFFVIILDD44 pKa = 3.58 FFIFARR50 pKa = 11.84 FSSSLIMKK58 pKa = 10.21 KK59 pKa = 10.0 SFQRR63 pKa = 11.84 SVTFGGMLLRR73 pKa = 11.84 YY74 pKa = 8.95 FIIFPLLYY82 pKa = 10.31 LGIRR86 pKa = 11.84 LAPNFMFAIISGAVWATFAFTITITTLLKK115 pKa = 10.53 EE116 pKa = 3.95 RR117 pKa = 11.84 SGWSMEE123 pKa = 3.95 EE124 pKa = 4.31 HH125 pKa = 6.35 NSSHH129 pKa = 6.11 GRR131 pKa = 3.43
Molecular weight: 15.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 10.101
IPC_protein 10.935
Toseland 10.877
ProMoST 11.038
Dawson 10.979
Bjellqvist 10.73
Wikipedia 11.228
Rodwell 11.184
Grimsley 11.038
Solomon 11.125
Lehninger 11.082
Nozaki 10.847
DTASelect 10.73
Thurlkill 10.877
EMBOSS 11.286
Sillero 10.921
Patrickios 10.95
IPC_peptide 11.125
IPC2_peptide 9.663
IPC2.peptide.svr19 8.301
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1617
0
1617
494852
38
1482
306.0
34.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.519 ± 0.06
0.967 ± 0.027
4.798 ± 0.038
8.094 ± 0.084
5.224 ± 0.059
6.764 ± 0.051
1.518 ± 0.025
8.594 ± 0.062
9.375 ± 0.07
9.492 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.123 ± 0.025
4.08 ± 0.042
3.97 ± 0.036
1.981 ± 0.026
4.515 ± 0.047
5.495 ± 0.04
4.844 ± 0.038
7.364 ± 0.049
0.77 ± 0.018
3.512 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here