Penicillium polonicum
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10627 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V6N734|A0A1V6N734_9EURO Uncharacterized protein OS=Penicillium polonicum OX=60169 GN=PENPOL_c023G07513 PE=4 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 VQQLLPLCGLLLTSVAIASKK22 pKa = 10.68 LDD24 pKa = 3.54 NDD26 pKa = 4.87 DD27 pKa = 3.41 VPNRR31 pKa = 11.84 CWPACGPVVGIAKK44 pKa = 10.16 SCDD47 pKa = 3.49 DD48 pKa = 3.24 QHH50 pKa = 6.87 EE51 pKa = 4.38 RR52 pKa = 11.84 DD53 pKa = 3.76 SSEE56 pKa = 3.82 IQCICNWDD64 pKa = 3.5 AAKK67 pKa = 8.97 TQIPLCSACITQYY80 pKa = 9.2 QTDD83 pKa = 3.85 KK84 pKa = 10.48 RR85 pKa = 11.84 NHH87 pKa = 6.59 NITHH91 pKa = 6.89 NDD93 pKa = 3.42 HH94 pKa = 7.7 DD95 pKa = 6.23 DD96 pKa = 5.2 DD97 pKa = 7.63 DD98 pKa = 7.37 DD99 pKa = 7.69 DD100 pKa = 7.66 DD101 pKa = 7.62 DD102 pKa = 7.63 DD103 pKa = 7.63 DD104 pKa = 7.57 DD105 pKa = 6.61 DD106 pKa = 6.6 DD107 pKa = 6.14 NEE109 pKa = 4.5 ALDD112 pKa = 4.81 LVHH115 pKa = 6.72 SCSLTTTTYY124 pKa = 10.82 NPAATSVTGTSTTTAGSNTATTTGTSSTTTSNGASGTNSQDD165 pKa = 3.15 STSSVSSGSSNLASASSGSASSSNPTPDD193 pKa = 3.0 AAAGISAPGAASMAGVMGLMAFAWLL218 pKa = 3.69
Molecular weight: 22.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.821
IPC_protein 3.859
Toseland 3.617
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.872
Rodwell 3.681
Grimsley 3.528
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.342
Thurlkill 3.694
EMBOSS 3.884
Sillero 3.999
Patrickios 0.846
IPC_peptide 3.872
IPC2_peptide 3.961
IPC2.peptide.svr19 3.841
Protein with the highest isoelectric point:
>tr|A0A1V6NUI8|A0A1V6NUI8_9EURO Uncharacterized protein OS=Penicillium polonicum OX=60169 GN=PENPOL_c003G05735 PE=3 SV=1
MM1 pKa = 7.89 PSNKK5 pKa = 8.19 TFRR8 pKa = 11.84 TKK10 pKa = 10.49 QKK12 pKa = 9.65 LAKK15 pKa = 9.74 AQRR18 pKa = 11.84 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 4.53
Molecular weight: 4.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 10.965
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.457
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.193
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.074
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10627
0
10627
5256275
8
6527
494.6
54.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.406 ± 0.02
1.246 ± 0.008
5.651 ± 0.016
6.093 ± 0.026
3.83 ± 0.015
6.772 ± 0.019
2.44 ± 0.01
5.148 ± 0.018
4.596 ± 0.019
9.019 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.263 ± 0.008
3.743 ± 0.012
6.047 ± 0.03
4.055 ± 0.015
5.879 ± 0.019
8.348 ± 0.024
6.024 ± 0.014
6.143 ± 0.018
1.495 ± 0.009
2.8 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here