Rudbeckia flower distortion virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Ruflodivirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8Y873|B8Y873_9VIRU Uncharacterized protein OS=Rudbeckia flower distortion virus OX=587370 PE=4 SV=1
MM1 pKa = 7.99DD2 pKa = 5.08NDD4 pKa = 3.84FLEE7 pKa = 4.12LLYY10 pKa = 10.83RR11 pKa = 11.84NKK13 pKa = 10.16QPLCEE18 pKa = 4.15NNAIRR23 pKa = 11.84YY24 pKa = 7.75ANKK27 pKa = 9.73VDD29 pKa = 3.81DD30 pKa = 4.25LLYY33 pKa = 9.39YY34 pKa = 10.28QEE36 pKa = 4.54FLVLKK41 pKa = 10.53AIDD44 pKa = 3.74EE45 pKa = 4.96LIFMRR50 pKa = 11.84NCQQTYY56 pKa = 7.69NQVYY60 pKa = 10.67SRR62 pKa = 11.84GSDD65 pKa = 3.19LNRR68 pKa = 11.84FYY70 pKa = 11.5GLQLEE75 pKa = 4.46SEE77 pKa = 4.39IKK79 pKa = 9.95IEE81 pKa = 3.92KK82 pKa = 10.62LEE84 pKa = 4.81DD85 pKa = 3.02IRR87 pKa = 11.84KK88 pKa = 8.1QFLMHH93 pKa = 7.06SDD95 pKa = 3.36NNYY98 pKa = 9.9CLHH101 pKa = 6.49NNLPRR106 pKa = 11.84GVQPPITSVYY116 pKa = 10.94NGTTTFKK123 pKa = 11.22GNYY126 pKa = 8.22SEE128 pKa = 6.12NYY130 pKa = 9.61LCSLDD135 pKa = 3.58SRR137 pKa = 11.84IVEE140 pKa = 4.16KK141 pKa = 10.58RR142 pKa = 11.84SKK144 pKa = 10.57EE145 pKa = 3.93LLEE148 pKa = 4.12LRR150 pKa = 11.84QALSEE155 pKa = 4.21SLPLLSSSEE164 pKa = 4.53DD165 pKa = 3.67EE166 pKa = 4.69NSDD169 pKa = 3.25

Molecular weight: 19.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8Y872|B8Y872_9VIRU Putative translational transactivator OS=Rudbeckia flower distortion virus OX=587370 PE=4 SV=1
MM1 pKa = 7.2TKK3 pKa = 10.51SKK5 pKa = 9.04ITLLMNLNLKK15 pKa = 9.37TSGKK19 pKa = 7.24STKK22 pKa = 8.93TNPYY26 pKa = 7.48STYY29 pKa = 8.96VTVGFYY35 pKa = 10.8FNGYY39 pKa = 9.92KK40 pKa = 10.38GFHH43 pKa = 5.58LHH45 pKa = 7.14AYY47 pKa = 9.05VDD49 pKa = 4.05TGATSCTASPHH60 pKa = 6.6IIPTEE65 pKa = 3.69LWEE68 pKa = 4.35EE69 pKa = 4.09LPKK72 pKa = 10.63PILVNIANDD81 pKa = 3.6TNIEE85 pKa = 3.62IRR87 pKa = 11.84YY88 pKa = 4.93VTRR91 pKa = 11.84NLKK94 pKa = 10.53VSMTDD99 pKa = 3.14TNGNKK104 pKa = 8.34HH105 pKa = 5.47TFIIPTVYY113 pKa = 10.07QQNTGMDD120 pKa = 4.44FIFGQNFLKK129 pKa = 10.66LYY131 pKa = 10.68RR132 pKa = 11.84PFTQDD137 pKa = 2.75LYY139 pKa = 11.2EE140 pKa = 5.49IYY142 pKa = 9.54LTPKK146 pKa = 9.93LLKK149 pKa = 10.55FKK151 pKa = 10.14IQQRR155 pKa = 11.84AFKK158 pKa = 9.83QASPGFLDD166 pKa = 4.86SIRR169 pKa = 11.84KK170 pKa = 7.55QKK172 pKa = 10.45RR173 pKa = 11.84GEE175 pKa = 4.33IISKK179 pKa = 10.05HH180 pKa = 4.48ATNPINITKK189 pKa = 10.56LEE191 pKa = 4.55DD192 pKa = 3.14NLRR195 pKa = 11.84IVEE198 pKa = 4.63TILEE202 pKa = 4.34DD203 pKa = 4.05SNSKK207 pKa = 8.12TLSEE211 pKa = 4.26VSDD214 pKa = 3.81SKK216 pKa = 10.19TLSEE220 pKa = 5.32DD221 pKa = 3.07SDD223 pKa = 4.68DD224 pKa = 3.97YY225 pKa = 11.25FVYY228 pKa = 10.61SIQRR232 pKa = 11.84HH233 pKa = 3.93NTIVNDD239 pKa = 4.82LLTQACSEE247 pKa = 4.24NPLDD251 pKa = 4.61EE252 pKa = 4.8NKK254 pKa = 10.14NHH256 pKa = 6.6NGLLAEE262 pKa = 4.72IKK264 pKa = 10.24LINPATTVNVKK275 pKa = 10.52SMAYY279 pKa = 10.06SPDD282 pKa = 3.41DD283 pKa = 3.31AVEE286 pKa = 3.99INKK289 pKa = 9.6QIQEE293 pKa = 4.1LLEE296 pKa = 4.18IKK298 pKa = 10.21VIRR301 pKa = 11.84PSRR304 pKa = 11.84SPHH307 pKa = 6.12SSPCFLVQNHH317 pKa = 5.5NEE319 pKa = 3.64IKK321 pKa = 10.12RR322 pKa = 11.84GKK324 pKa = 9.2KK325 pKa = 9.74RR326 pKa = 11.84LVINYY331 pKa = 7.48KK332 pKa = 10.2ALNAATISDD341 pKa = 4.65GYY343 pKa = 11.09LLPNKK348 pKa = 8.19EE349 pKa = 4.66TILTAIRR356 pKa = 11.84GRR358 pKa = 11.84KK359 pKa = 8.78YY360 pKa = 10.8FSTLDD365 pKa = 3.71CKK367 pKa = 11.09SGFWQIRR374 pKa = 11.84LNEE377 pKa = 3.95NSKK380 pKa = 10.32PLTAFSCPMGQYY392 pKa = 8.48EE393 pKa = 4.45WNVVPFGLKK402 pKa = 9.09QAPGLFQRR410 pKa = 11.84FMDD413 pKa = 3.83NSFKK417 pKa = 10.7EE418 pKa = 4.26YY419 pKa = 10.71SAFCAVYY426 pKa = 10.66VDD428 pKa = 5.31DD429 pKa = 5.26ILVFSKK435 pKa = 9.67TLDD438 pKa = 3.12EE439 pKa = 5.43HH440 pKa = 6.83YY441 pKa = 11.32DD442 pKa = 3.4HH443 pKa = 7.38LEE445 pKa = 3.87TVLRR449 pKa = 11.84KK450 pKa = 10.08CIEE453 pKa = 3.87TGIILSKK460 pKa = 10.71KK461 pKa = 8.14KK462 pKa = 10.95AEE464 pKa = 4.09VAKK467 pKa = 10.16TKK469 pKa = 10.4INYY472 pKa = 10.01LGFTISNGEE481 pKa = 3.95IEE483 pKa = 4.72LQSHH487 pKa = 5.67ILEE490 pKa = 4.69NIKK493 pKa = 10.42LFPSRR498 pKa = 11.84IPDD501 pKa = 3.34KK502 pKa = 11.02KK503 pKa = 10.4SLQRR507 pKa = 11.84FLGILTYY514 pKa = 10.83ADD516 pKa = 3.09QYY518 pKa = 10.68IRR520 pKa = 11.84KK521 pKa = 8.48LAEE524 pKa = 3.4WRR526 pKa = 11.84KK527 pKa = 8.54PLQRR531 pKa = 11.84KK532 pKa = 8.35LKK534 pKa = 10.37KK535 pKa = 9.77DD536 pKa = 4.01TVWEE540 pKa = 4.32WNDD543 pKa = 3.07SDD545 pKa = 3.7TQYY548 pKa = 11.32VEE550 pKa = 3.78KK551 pKa = 10.62FKK553 pKa = 11.33RR554 pKa = 11.84NLKK557 pKa = 9.52EE558 pKa = 3.81FPKK561 pKa = 10.27LHH563 pKa = 6.64HH564 pKa = 6.88PLPDD568 pKa = 3.22EE569 pKa = 4.02YY570 pKa = 11.37LIIEE574 pKa = 4.42TDD576 pKa = 3.07ASHH579 pKa = 5.86EE580 pKa = 4.04HH581 pKa = 5.8WAGVLKK587 pKa = 11.01SRR589 pKa = 11.84GTDD592 pKa = 3.06NLEE595 pKa = 3.65RR596 pKa = 11.84LCRR599 pKa = 11.84YY600 pKa = 7.89TSGSFKK606 pKa = 10.2PAEE609 pKa = 4.13INYY612 pKa = 9.4HH613 pKa = 4.93SNEE616 pKa = 4.07KK617 pKa = 10.24EE618 pKa = 3.87VLAVKK623 pKa = 9.46RR624 pKa = 11.84TITKK628 pKa = 9.96FKK630 pKa = 10.6GYY632 pKa = 9.46LASSEE637 pKa = 4.08FLVRR641 pKa = 11.84TDD643 pKa = 3.37NKK645 pKa = 10.13YY646 pKa = 8.35FTYY649 pKa = 10.6FLRR652 pKa = 11.84TSIKK656 pKa = 10.45DD657 pKa = 3.29DD658 pKa = 3.67YY659 pKa = 11.06KK660 pKa = 10.54QGRR663 pKa = 11.84LIRR666 pKa = 11.84WQQWFSHH673 pKa = 4.93YY674 pKa = 10.35KK675 pKa = 10.32FNVEE679 pKa = 4.28HH680 pKa = 7.03LAGTHH685 pKa = 6.09NFTADD690 pKa = 2.87SLTRR694 pKa = 11.84EE695 pKa = 4.62FANKK699 pKa = 9.67PPP701 pKa = 3.82

Molecular weight: 81.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2397

112

701

342.4

39.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.297 ± 0.631

1.585 ± 0.333

6.008 ± 0.352

7.468 ± 0.774

4.422 ± 0.344

3.504 ± 0.292

2.336 ± 0.264

7.968 ± 0.888

8.761 ± 0.498

9.595 ± 0.79

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.418 ± 0.311

7.134 ± 0.408

4.172 ± 0.345

4.172 ± 0.558

4.631 ± 0.497

7.217 ± 0.416

6.258 ± 0.606

3.963 ± 0.21

0.876 ± 0.185

4.214 ± 0.376
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski