Beihai horseshoe crab virus 1
Average proteome isoelectric point is 7.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFV0|A0A1L3KFV0_9VIRU Uncharacterized protein OS=Beihai horseshoe crab virus 1 OX=1922392 PE=4 SV=1
MM1 pKa = 7.78 RR2 pKa = 11.84 PSQTNTAGPEE12 pKa = 3.44 WDD14 pKa = 3.89 RR15 pKa = 11.84 YY16 pKa = 8.27 LTEE19 pKa = 4.75 LGRR22 pKa = 11.84 LATAIGRR29 pKa = 11.84 VPKK32 pKa = 10.66 ASTKK36 pKa = 10.68 AILAGLKK43 pKa = 9.6 GRR45 pKa = 11.84 RR46 pKa = 11.84 KK47 pKa = 9.51 RR48 pKa = 11.84 RR49 pKa = 11.84 YY50 pKa = 7.78 HH51 pKa = 5.92 QGFSTLIAKK60 pKa = 9.97 GIGRR64 pKa = 11.84 RR65 pKa = 11.84 DD66 pKa = 3.42 AYY68 pKa = 9.69 IKK70 pKa = 10.88 SMQKK74 pKa = 10.21 LEE76 pKa = 4.19 LYY78 pKa = 9.96 EE79 pKa = 4.08 VEE81 pKa = 4.87 KK82 pKa = 11.01 LDD84 pKa = 3.97 SKK86 pKa = 10.56 EE87 pKa = 3.76 NRR89 pKa = 11.84 AIQYY93 pKa = 10.33 RR94 pKa = 11.84 SVIYY98 pKa = 9.37 NAAMARR104 pKa = 11.84 EE105 pKa = 4.08 LHH107 pKa = 6.38 CFSKK111 pKa = 10.74 RR112 pKa = 11.84 LAVVHH117 pKa = 6.15 SLNDD121 pKa = 3.49 TNTPITTKK129 pKa = 10.7 GMTPRR134 pKa = 11.84 QMAEE138 pKa = 3.86 KK139 pKa = 10.05 IVFEE143 pKa = 4.16 WSTYY147 pKa = 8.35 EE148 pKa = 4.81 DD149 pKa = 4.04 PVALLLDD156 pKa = 4.28 FSRR159 pKa = 11.84 MDD161 pKa = 3.17 GSYY164 pKa = 10.58 TIDD167 pKa = 3.47 TIRR170 pKa = 11.84 AEE172 pKa = 4.05 HH173 pKa = 6.54 AVYY176 pKa = 10.11 KK177 pKa = 9.81 YY178 pKa = 10.29 CRR180 pKa = 11.84 PSKK183 pKa = 10.27 RR184 pKa = 11.84 LSWLLKK190 pKa = 8.19 QQEE193 pKa = 3.92 RR194 pKa = 11.84 SYY196 pKa = 11.33 GVTEE200 pKa = 4.23 GGVKK204 pKa = 10.1 YY205 pKa = 8.75 VTYY208 pKa = 10.44 GKK210 pKa = 10.32 RR211 pKa = 11.84 SSGDD215 pKa = 3.4 YY216 pKa = 8.49 NTSDD220 pKa = 3.28 GNTITNYY227 pKa = 10.54 AATSSWCGSVRR238 pKa = 11.84 RR239 pKa = 11.84 SFTGIGDD246 pKa = 4.05 DD247 pKa = 3.27 NVVIMEE253 pKa = 4.24 RR254 pKa = 11.84 NSYY257 pKa = 10.48 YY258 pKa = 10.67 SLPPVEE264 pKa = 5.77 GFFLEE269 pKa = 4.24 LGFVVEE275 pKa = 4.17 PVIAEE280 pKa = 4.1 RR281 pKa = 11.84 VCDD284 pKa = 4.24 IEE286 pKa = 4.41 YY287 pKa = 9.94 CQSKK291 pKa = 10.53 IMFDD295 pKa = 3.52 DD296 pKa = 3.26 QGPIFVRR303 pKa = 11.84 DD304 pKa = 3.3 WKK306 pKa = 11.03 KK307 pKa = 10.4 VVEE310 pKa = 4.38 TVKK313 pKa = 10.6 LSPVHH318 pKa = 6.38 YY319 pKa = 9.93 DD320 pKa = 3.23 ARR322 pKa = 11.84 LCKK325 pKa = 9.74 QVALANLLCQLAIYY339 pKa = 10.5 NDD341 pKa = 3.44 IPIIGPYY348 pKa = 9.89 CYY350 pKa = 10.17 RR351 pKa = 11.84 NLKK354 pKa = 10.04 HH355 pKa = 6.53 YY356 pKa = 11.04 DD357 pKa = 3.19 GVTPRR362 pKa = 11.84 LPEE365 pKa = 3.95 YY366 pKa = 11.06 LDD368 pKa = 3.99 FKK370 pKa = 10.39 MNYY373 pKa = 8.23 IKK375 pKa = 10.66 RR376 pKa = 11.84 VDD378 pKa = 3.24 ISKK381 pKa = 11.13 LEE383 pKa = 3.81 VHH385 pKa = 6.19 EE386 pKa = 4.04 PKK388 pKa = 10.22 IRR390 pKa = 11.84 DD391 pKa = 3.63 TVRR394 pKa = 11.84 MSFHH398 pKa = 6.2 RR399 pKa = 11.84 TFGVDD404 pKa = 3.07 PTMQMWLEE412 pKa = 4.07 EE413 pKa = 4.25 SLDD416 pKa = 3.75 YY417 pKa = 11.47 CPVEE421 pKa = 4.64 NNAKK425 pKa = 9.27 QPKK428 pKa = 9.43 IKK430 pKa = 9.11 VTKK433 pKa = 9.89 SLEE436 pKa = 4.19 EE437 pKa = 3.72 IVDD440 pKa = 4.04 FEE442 pKa = 5.18 EE443 pKa = 4.56 NFTVEE448 pKa = 3.95 PCDD451 pKa = 4.27 CGEE454 pKa = 4.38 CPDD457 pKa = 4.06 YY458 pKa = 11.29 SWNEE462 pKa = 3.76 EE463 pKa = 4.13 LLGHH467 pKa = 6.84 LRR469 pKa = 11.84 EE470 pKa = 4.6 CC471 pKa = 5.06
Molecular weight: 54.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.865
IPC2_protein 7.176
IPC_protein 7.059
Toseland 6.751
ProMoST 7.658
Dawson 7.732
Bjellqvist 8.097
Wikipedia 7.644
Rodwell 7.746
Grimsley 6.795
Solomon 7.775
Lehninger 7.79
Nozaki 8.287
DTASelect 7.878
Thurlkill 7.922
EMBOSS 7.951
Sillero 8.244
Patrickios 4.05
IPC_peptide 7.761
IPC2_peptide 7.278
IPC2.peptide.svr19 7.271
Protein with the highest isoelectric point:
>tr|A0A1L3KG54|A0A1L3KG54_9VIRU Capsid protein OS=Beihai horseshoe crab virus 1 OX=1922392 PE=4 SV=1
MM1 pKa = 7.35 SNKK4 pKa = 7.94 TVKK7 pKa = 10.41 PGVVKK12 pKa = 10.5 RR13 pKa = 11.84 KK14 pKa = 10.09 KK15 pKa = 8.48 PARR18 pKa = 11.84 PNRR21 pKa = 11.84 EE22 pKa = 3.65 LKK24 pKa = 9.94 QAPKK28 pKa = 10.38 SDD30 pKa = 4.35 DD31 pKa = 3.43 PALPLTVVINGVEE44 pKa = 4.41 VTSGPGTVISIWRR57 pKa = 11.84 GGKK60 pKa = 8.57 RR61 pKa = 11.84 WYY63 pKa = 8.84 THH65 pKa = 6.1 GSPPKK70 pKa = 8.69 SGSVGTVVPGVSYY83 pKa = 11.14 AEE85 pKa = 4.33 KK86 pKa = 10.69 VSTDD90 pKa = 3.22 PKK92 pKa = 10.21 EE93 pKa = 3.99 EE94 pKa = 4.01 SRR96 pKa = 11.84 KK97 pKa = 9.87 KK98 pKa = 10.16 DD99 pKa = 3.19 TVPPVTAVKK108 pKa = 10.48 VHH110 pKa = 6.76 TISSADD116 pKa = 3.46 EE117 pKa = 4.2 KK118 pKa = 10.94 PPSRR122 pKa = 11.84 RR123 pKa = 11.84 QKK125 pKa = 9.65 RR126 pKa = 11.84 LVVAEE131 pKa = 3.88 QATAEE136 pKa = 4.28 VAKK139 pKa = 10.94 SGGSWNEE146 pKa = 3.06 IFRR149 pKa = 11.84 RR150 pKa = 11.84 LWSARR155 pKa = 11.84 SDD157 pKa = 3.3 KK158 pKa = 11.15 GPKK161 pKa = 9.66 QKK163 pKa = 10.27 MKK165 pKa = 10.66 DD166 pKa = 2.84 WAKK169 pKa = 10.24 QVEE172 pKa = 4.52 KK173 pKa = 11.01 ADD175 pKa = 3.8 FLAARR180 pKa = 11.84 EE181 pKa = 4.11 SHH183 pKa = 6.51 LGKK186 pKa = 10.78 ANGKK190 pKa = 4.22 TLKK193 pKa = 10.13 RR194 pKa = 11.84 RR195 pKa = 11.84 RR196 pKa = 11.84 QRR198 pKa = 11.84 LAAKK202 pKa = 10.32 NKK204 pKa = 9.99 DD205 pKa = 3.33 RR206 pKa = 11.84 VTAWVADD213 pKa = 3.78 QNRR216 pKa = 11.84 QNLTDD221 pKa = 3.81 LQTIPEE227 pKa = 4.66 RR228 pKa = 11.84 PPTPHH233 pKa = 7.49 PIILDD238 pKa = 3.75 KK239 pKa = 11.31 LPEE242 pKa = 4.78 DD243 pKa = 4.35 LDD245 pKa = 3.88 EE246 pKa = 4.64 VDD248 pKa = 3.57 WPKK251 pKa = 11.12 GEE253 pKa = 4.54 PMAQQVPLPQRR264 pKa = 11.84 EE265 pKa = 4.18 RR266 pKa = 11.84 FRR268 pKa = 11.84 KK269 pKa = 10.24 DD270 pKa = 2.97 PLIPVGKK277 pKa = 9.96 KK278 pKa = 10.28 GMAKK282 pKa = 10.2 LVLDD286 pKa = 3.44 NAEE289 pKa = 4.15 RR290 pKa = 11.84 EE291 pKa = 4.04 RR292 pKa = 11.84 AALYY296 pKa = 10.4 RR297 pKa = 11.84 LARR300 pKa = 11.84 EE301 pKa = 4.12 QQAEE305 pKa = 4.2 AEE307 pKa = 4.36 KK308 pKa = 11.19 ANAALAEE315 pKa = 4.62 KK316 pKa = 10.05 IRR318 pKa = 11.84 QRR320 pKa = 11.84 EE321 pKa = 3.8 KK322 pKa = 9.98 SEE324 pKa = 3.54 EE325 pKa = 3.72 RR326 pKa = 11.84 RR327 pKa = 11.84 FNLLHH332 pKa = 6.12 KK333 pKa = 10.0 RR334 pKa = 11.84 EE335 pKa = 4.38 TIGPPIKK342 pKa = 9.72 TKK344 pKa = 10.67 SKK346 pKa = 10.26 EE347 pKa = 3.98 LVPLGDD353 pKa = 3.76 LSGLRR358 pKa = 11.84 AHH360 pKa = 6.99 LQAKK364 pKa = 9.14 VAFKK368 pKa = 10.6 PRR370 pKa = 11.84 DD371 pKa = 3.42 HH372 pKa = 6.88 RR373 pKa = 11.84 TPAWLSFEE381 pKa = 4.08 AQRR384 pKa = 11.84 WVADD388 pKa = 3.62 HH389 pKa = 7.28 DD390 pKa = 4.07 TSQWDD395 pKa = 3.55 PLALQTAVEE404 pKa = 4.15 MMIVTCLEE412 pKa = 3.93 PRR414 pKa = 11.84 SVEE417 pKa = 3.9 LSSEE421 pKa = 4.15 KK422 pKa = 10.12 TLSTRR427 pKa = 11.84 SLSKK431 pKa = 10.82 LNSIYY436 pKa = 10.44 RR437 pKa = 11.84 WGKK440 pKa = 6.15 TSRR443 pKa = 11.84 FEE445 pKa = 4.21 SWRR448 pKa = 11.84 LCADD452 pKa = 4.19 FAGGFLGCRR461 pKa = 11.84 VASVIFEE468 pKa = 3.75 EE469 pKa = 4.08 RR470 pKa = 11.84 HH471 pKa = 5.66 YY472 pKa = 10.74 IRR474 pKa = 11.84 HH475 pKa = 6.87 LINSFVARR483 pKa = 11.84 QCVKK487 pKa = 10.08 MGWYY491 pKa = 10.1 KK492 pKa = 9.41 MAYY495 pKa = 9.85 RR496 pKa = 11.84 FDD498 pKa = 3.87 KK499 pKa = 10.68 PVTLGSCVRR508 pKa = 11.84 DD509 pKa = 3.26 NLEE512 pKa = 3.9 RR513 pKa = 11.84 LNPHH517 pKa = 7.47 LILKK521 pKa = 9.52 AKK523 pKa = 9.72 KK524 pKa = 10.18 ALISLTDD531 pKa = 3.58 RR532 pKa = 11.84 FVLALKK538 pKa = 9.79 MKK540 pKa = 10.46 LGYY543 pKa = 9.47 QPPVPPPVRR552 pKa = 11.84 LEE554 pKa = 3.96 VRR556 pKa = 11.84 VARR559 pKa = 11.84 YY560 pKa = 9.77 LMSKK564 pKa = 8.22 VTLRR568 pKa = 11.84 GAVLAAGFFCGTYY581 pKa = 9.75 IIYY584 pKa = 10.34 RR585 pKa = 11.84 KK586 pKa = 9.46 VGLRR590 pKa = 11.84 RR591 pKa = 11.84 RR592 pKa = 11.84 VRR594 pKa = 11.84 HH595 pKa = 6.19 PEE597 pKa = 3.68 QEE599 pKa = 4.46 SNN601 pKa = 3.29
Molecular weight: 68.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.19
IPC2_protein 9.428
IPC_protein 9.516
Toseland 10.526
ProMoST 10.028
Dawson 10.599
Bjellqvist 10.204
Wikipedia 10.73
Rodwell 11.096
Grimsley 10.628
Solomon 10.643
Lehninger 10.628
Nozaki 10.496
DTASelect 10.204
Thurlkill 10.496
EMBOSS 10.891
Sillero 10.526
Patrickios 10.789
IPC_peptide 10.657
IPC2_peptide 8.741
IPC2.peptide.svr19 8.61
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1523
451
601
507.7
56.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.536 ± 1.628
1.773 ± 0.43
4.662 ± 0.467
5.844 ± 1.07
2.823 ± 0.166
6.303 ± 0.754
2.232 ± 0.265
4.596 ± 0.541
7.157 ± 1.048
7.879 ± 0.605
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.232 ± 0.255
3.414 ± 0.337
5.975 ± 0.599
3.414 ± 0.279
7.026 ± 1.513
7.551 ± 1.15
6.172 ± 0.82
7.223 ± 0.422
1.97 ± 0.205
3.217 ± 1.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here