Pimoid spider associated circular virus 2
Average proteome isoelectric point is 8.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPD3|A0A346BPD3_9VIRU Putative capsid protein OS=Pimoid spider associated circular virus 2 OX=2293298 PE=4 SV=1
MM1 pKa = 7.44 SCKK4 pKa = 9.85 NWCFTWNNPDD14 pKa = 3.53 LGYY17 pKa = 10.67 EE18 pKa = 4.1 ALLEE22 pKa = 4.12 LLSSKK27 pKa = 10.58 SSYY30 pKa = 10.89 CIFQKK35 pKa = 10.71 EE36 pKa = 3.77 QGANGTVHH44 pKa = 6.56 FQGFVQFTSKK54 pKa = 10.19 QRR56 pKa = 11.84 LSSLKK61 pKa = 10.24 KK62 pKa = 9.91 VLQSAHH68 pKa = 4.86 WEE70 pKa = 4.28 RR71 pKa = 11.84 TKK73 pKa = 10.59 GTAEE77 pKa = 4.05 EE78 pKa = 4.02 NRR80 pKa = 11.84 DD81 pKa = 3.7 YY82 pKa = 11.13 CSKK85 pKa = 11.03 ADD87 pKa = 3.81 SRR89 pKa = 11.84 VDD91 pKa = 3.89 GPWSSGRR98 pKa = 11.84 IVVKK102 pKa = 9.11 GQRR105 pKa = 11.84 RR106 pKa = 11.84 DD107 pKa = 3.16 IEE109 pKa = 4.19 EE110 pKa = 3.85 FRR112 pKa = 11.84 DD113 pKa = 3.64 AVLEE117 pKa = 4.47 GSCNLDD123 pKa = 2.78 LVMSFPGQVARR134 pKa = 11.84 FPKK137 pKa = 10.57 FIDD140 pKa = 4.01 FVRR143 pKa = 11.84 SAQSVRR149 pKa = 11.84 RR150 pKa = 11.84 VEE152 pKa = 4.3 KK153 pKa = 10.32 PVVEE157 pKa = 4.4 CFYY160 pKa = 11.18 GPSGSGKK167 pKa = 6.93 TRR169 pKa = 11.84 RR170 pKa = 11.84 AMAVSGFDD178 pKa = 3.11 STFVVSRR185 pKa = 11.84 PDD187 pKa = 2.91 SGRR190 pKa = 11.84 PLWWDD195 pKa = 3.18 GFDD198 pKa = 3.59 PRR200 pKa = 11.84 IHH202 pKa = 5.88 ITVVLDD208 pKa = 5.37 DD209 pKa = 4.31 FYY211 pKa = 11.95 GWIPWSFLLQLIDD224 pKa = 4.21 RR225 pKa = 11.84 YY226 pKa = 10.11 PFQVEE231 pKa = 4.12 KK232 pKa = 10.77 KK233 pKa = 9.47 GGKK236 pKa = 8.97 IEE238 pKa = 4.4 FNCKK242 pKa = 9.54 NVIFTSNQHH251 pKa = 5.17 PSKK254 pKa = 9.96 WYY256 pKa = 10.24 KK257 pKa = 10.46 SVPNDD262 pKa = 3.96 DD263 pKa = 3.93 LTPLLRR269 pKa = 11.84 RR270 pKa = 11.84 IDD272 pKa = 4.14 NIVLMEE278 pKa = 4.12
Molecular weight: 31.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.782
IPC2_protein 7.995
IPC_protein 7.892
Toseland 7.644
ProMoST 8.419
Dawson 8.639
Bjellqvist 8.902
Wikipedia 8.58
Rodwell 8.682
Grimsley 7.556
Solomon 8.77
Lehninger 8.785
Nozaki 9.151
DTASelect 8.653
Thurlkill 8.741
EMBOSS 8.843
Sillero 9.048
Patrickios 4.698
IPC_peptide 8.77
IPC2_peptide 7.79
IPC2.peptide.svr19 7.92
Protein with the highest isoelectric point:
>tr|A0A346BPD3|A0A346BPD3_9VIRU Putative capsid protein OS=Pimoid spider associated circular virus 2 OX=2293298 PE=4 SV=1
MM1 pKa = 7.76 VYY3 pKa = 9.0 GTLAAVAGNYY13 pKa = 7.72 AAKK16 pKa = 8.96 RR17 pKa = 11.84 TYY19 pKa = 11.22 DD20 pKa = 3.45 EE21 pKa = 3.78 MHH23 pKa = 5.75 NRR25 pKa = 11.84 IWKK28 pKa = 9.95 HH29 pKa = 4.84 SGHH32 pKa = 6.26 YY33 pKa = 10.96 GNDD36 pKa = 3.24 EE37 pKa = 4.23 RR38 pKa = 11.84 DD39 pKa = 3.75 TNAGPSVGGYY49 pKa = 10.19 GGGHH53 pKa = 6.18 IKK55 pKa = 10.58 SVFRR59 pKa = 11.84 AKK61 pKa = 10.22 KK62 pKa = 9.48 HH63 pKa = 4.71 SKK65 pKa = 10.21 KK66 pKa = 9.74 RR67 pKa = 11.84 DD68 pKa = 3.52 KK69 pKa = 11.47 SCGTLSAASLPAIRR83 pKa = 11.84 TSSGGICKK91 pKa = 9.88 KK92 pKa = 10.94 VYY94 pKa = 10.66 DD95 pKa = 4.45 FLVNTNGPIWNQSTGFTTAGPTATITSLGFSPMSVPFMGTYY136 pKa = 8.31 WKK138 pKa = 10.45 CLNAVEE144 pKa = 5.07 HH145 pKa = 6.28 VLDD148 pKa = 4.72 PKK150 pKa = 9.54 PTAGSATSVGQVMSFEE166 pKa = 4.54 KK167 pKa = 10.55 KK168 pKa = 9.34 FPHH171 pKa = 7.0 KK172 pKa = 10.68 GRR174 pKa = 11.84 IHH176 pKa = 7.17 LEE178 pKa = 3.66 QLRR181 pKa = 11.84 GNVCVAGLSRR191 pKa = 11.84 CYY193 pKa = 10.31 LIKK196 pKa = 10.27 EE197 pKa = 4.3 APLGIDD203 pKa = 3.39 PSKK206 pKa = 10.87 AGPLGTIMPAFGEE219 pKa = 4.33 SSGIATASKK228 pKa = 9.74 IHH230 pKa = 5.88 GVTYY234 pKa = 9.46 TYY236 pKa = 10.0 TKK238 pKa = 10.58 RR239 pKa = 11.84 FMMKK243 pKa = 10.07 PEE245 pKa = 4.06 PTSTLMARR253 pKa = 11.84 PRR255 pKa = 11.84 QQEE258 pKa = 3.88 NAA260 pKa = 3.56
Molecular weight: 27.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.123
IPC2_protein 9.18
IPC_protein 9.092
Toseland 9.823
ProMoST 9.531
Dawson 10.058
Bjellqvist 9.75
Wikipedia 10.218
Rodwell 10.482
Grimsley 10.116
Solomon 10.072
Lehninger 10.043
Nozaki 9.897
DTASelect 9.721
Thurlkill 9.897
EMBOSS 10.233
Sillero 9.984
Patrickios 9.692
IPC_peptide 10.072
IPC2_peptide 8.448
IPC2.peptide.svr19 8.075
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
538
260
278
269.0
29.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.134 ± 1.836
2.23 ± 0.208
4.647 ± 1.323
4.275 ± 0.551
5.204 ± 1.179
8.922 ± 1.51
2.416 ± 0.707
4.461 ± 0.104
7.063 ± 0.426
6.32 ± 0.633
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.416 ± 0.707
3.903 ± 0.039
5.576 ± 0.391
3.16 ± 0.837
5.948 ± 0.902
9.108 ± 0.437
5.948 ± 1.701
6.877 ± 1.01
2.23 ± 0.729
3.16 ± 0.464
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here