Candidatus Prometheoarchaeum syntrophicum
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3933 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B9DD94|A0A5B9DD94_9ARCH Uncharacterized protein OS=Candidatus Prometheoarchaeum syntrophicum OX=2594042 GN=DSAG12_03121 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.15 IVIAFITLSVALTSIVGGISIVLIASDD29 pKa = 4.1 IEE31 pKa = 4.46 NNFQINEE38 pKa = 3.95 SDD40 pKa = 3.06 IQANFGASGNNISLSFNFTNDD61 pKa = 2.59 GYY63 pKa = 11.31 FAFEE67 pKa = 4.27 NFTVSLKK74 pKa = 10.13 IDD76 pKa = 3.72 MEE78 pKa = 4.56 NKK80 pKa = 10.1 SSADD84 pKa = 3.28 QIIFLDD90 pKa = 3.67 RR91 pKa = 11.84 EE92 pKa = 4.36 IYY94 pKa = 10.64 QNTLEE99 pKa = 4.65 GKK101 pKa = 8.66 NSYY104 pKa = 9.99 MIDD107 pKa = 3.54 LVATGEE113 pKa = 4.17 FFNNQSLLGDD123 pKa = 3.9 MGGTWNDD130 pKa = 3.65 PEE132 pKa = 4.18 VDD134 pKa = 3.86 ALIATNSSLEE144 pKa = 3.87 PLLRR148 pKa = 11.84 PLSFPYY154 pKa = 10.2 ILNNYY159 pKa = 8.8 DD160 pKa = 3.29 INVTLSIASKK170 pKa = 10.77 YY171 pKa = 10.48 NLGLIDD177 pKa = 5.12 FGLDD181 pKa = 3.24 LEE183 pKa = 4.75 FSIAYY188 pKa = 9.61 DD189 pKa = 3.78 DD190 pKa = 4.35 YY191 pKa = 11.75 FSISYY196 pKa = 9.52 SQYY199 pKa = 10.45 KK200 pKa = 9.98 QNLKK204 pKa = 10.64 DD205 pKa = 4.28 DD206 pKa = 4.09 YY207 pKa = 11.74 GLL209 pKa = 3.96
Molecular weight: 23.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.528
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.872
Patrickios 0.998
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A5B9D8H9|A0A5B9D8H9_9ARCH Uncharacterized protein OS=Candidatus Prometheoarchaeum syntrophicum OX=2594042 GN=DSAG12_01394 PE=4 SV=1
MM1 pKa = 7.3 NEE3 pKa = 3.6 KK4 pKa = 10.45 RR5 pKa = 11.84 MNTSISLKK13 pKa = 9.73 GKK15 pKa = 9.19 GSRR18 pKa = 11.84 VCRR21 pKa = 11.84 RR22 pKa = 11.84 CGSHH26 pKa = 6.74 RR27 pKa = 11.84 GLIRR31 pKa = 11.84 QYY33 pKa = 11.46 GLNLCRR39 pKa = 11.84 RR40 pKa = 11.84 CFRR43 pKa = 11.84 EE44 pKa = 3.83 VANDD48 pKa = 3.73 LGFKK52 pKa = 10.29 KK53 pKa = 10.67 YY54 pKa = 10.72
Molecular weight: 6.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.736
IPC_protein 10.584
Toseland 10.833
ProMoST 10.57
Dawson 10.906
Bjellqvist 10.643
Wikipedia 11.125
Rodwell 11.125
Grimsley 10.95
Solomon 11.067
Lehninger 11.023
Nozaki 10.847
DTASelect 10.628
Thurlkill 10.833
EMBOSS 11.242
Sillero 10.862
Patrickios 10.906
IPC_peptide 11.067
IPC2_peptide 9.984
IPC2.peptide.svr19 8.481
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3933
0
3933
1304535
30
10314
331.7
38.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.609 ± 0.036
1.039 ± 0.015
5.304 ± 0.043
6.931 ± 0.046
5.651 ± 0.041
5.595 ± 0.04
1.775 ± 0.015
10.314 ± 0.051
8.621 ± 0.082
9.758 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.286 ± 0.02
6.066 ± 0.036
3.704 ± 0.023
3.229 ± 0.023
3.515 ± 0.028
6.772 ± 0.039
4.801 ± 0.035
4.881 ± 0.029
1.128 ± 0.016
4.02 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here