Saccharothrix sp. ST-888

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharothrix; unclassified Saccharothrix

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5936 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F0HJL0|A0A0F0HJL0_9PSEU Cation diffusion facilitator family transporter OS=Saccharothrix sp. ST-888 OX=1427391 GN=UK12_26585 PE=4 SV=1
MM1 pKa = 6.88TTPADD6 pKa = 3.91PAPEE10 pKa = 3.97ITPDD14 pKa = 2.95GHH16 pKa = 5.62TWLPQAYY23 pKa = 9.19CQTQQMWGPPDD34 pKa = 3.93PSQISPEE41 pKa = 3.8WPAWQLPDD49 pKa = 3.47WYY51 pKa = 10.3AGQTGMVQIAGQAGQPGVYY70 pKa = 9.78QYY72 pKa = 11.57VWDD75 pKa = 4.87RR76 pKa = 11.84WLPWQSLTDD85 pKa = 4.59RR86 pKa = 11.84IDD88 pKa = 4.08PAAEE92 pKa = 3.58WPGAVRR98 pKa = 11.84ATAADD103 pKa = 3.3MHH105 pKa = 6.58AALFPAPVPAPAVSEE120 pKa = 4.01PDD122 pKa = 3.24APLPADD128 pKa = 3.39ASEE131 pKa = 4.9PEE133 pKa = 4.34SEE135 pKa = 4.61APAVPPTSAPTLGTT149 pKa = 3.52

Molecular weight: 15.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F0HWH4|A0A0F0HWH4_9PSEU 3-hydroxyacyl-CoA dehydrogenase OS=Saccharothrix sp. ST-888 OX=1427391 GN=UK12_12895 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.43GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5936

0

5936

1759706

28

7401

296.4

31.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.857 ± 0.044

0.806 ± 0.008

5.7 ± 0.026

5.696 ± 0.032

2.757 ± 0.02

9.484 ± 0.029

2.269 ± 0.015

3.218 ± 0.024

1.958 ± 0.026

10.791 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.637 ± 0.013

1.805 ± 0.019

6.12 ± 0.03

3.079 ± 0.021

7.836 ± 0.037

5.142 ± 0.025

5.954 ± 0.027

8.371 ± 0.032

1.468 ± 0.013

2.051 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski