Tortoise picornavirus
Average proteome isoelectric point is 5.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7EQ87|A0A0A7EQ87_9PICO Capsid protein VP0 OS=Tortoise picornavirus OX=1578826 PE=4 SV=1
MM1 pKa = 6.93 EE2 pKa = 4.11 TTMEE6 pKa = 3.94 ISFDD10 pKa = 3.65 ASCPSSVCIFCLCQPCKK27 pKa = 10.37 CHH29 pKa = 7.53 LIHH32 pKa = 7.31 NEE34 pKa = 3.85 IYY36 pKa = 9.17 TICYY40 pKa = 7.65 FTDD43 pKa = 3.62 EE44 pKa = 4.63 EE45 pKa = 4.29 KK46 pKa = 10.81 KK47 pKa = 10.22 EE48 pKa = 3.92 YY49 pKa = 10.16 QIQGQGQSNARR60 pKa = 11.84 PNQQSNYY67 pKa = 8.42 GTITNNYY74 pKa = 9.05 YY75 pKa = 10.4 EE76 pKa = 4.21 NTYY79 pKa = 9.91 IASADD84 pKa = 3.45 MSTQANGNEE93 pKa = 3.93 GDD95 pKa = 3.82 IPDD98 pKa = 4.09 VPGIWSTLNGVVDD111 pKa = 4.29 TASAMAPLLLDD122 pKa = 3.33 QDD124 pKa = 4.32 TEE126 pKa = 4.32 EE127 pKa = 4.36 TTNLSDD133 pKa = 3.52 RR134 pKa = 11.84 VKK136 pKa = 10.38 TSIHH140 pKa = 6.35 GNTSVGTQSSVGTVIGYY157 pKa = 9.74 KK158 pKa = 9.69 KK159 pKa = 10.71 EE160 pKa = 3.92 KK161 pKa = 8.9 DD162 pKa = 3.45 TNPISSCADD171 pKa = 3.18 APTVASFAMEE181 pKa = 3.85 RR182 pKa = 11.84 AFTQTMGTWSKK193 pKa = 10.18 TNDD196 pKa = 2.98 VYY198 pKa = 11.34 QYY200 pKa = 11.09 IHH202 pKa = 6.74 ICLPSGIDD210 pKa = 3.43 DD211 pKa = 4.1 GGVFSGVLNRR221 pKa = 11.84 HH222 pKa = 5.46 YY223 pKa = 11.22 LMKK226 pKa = 10.35 CGYY229 pKa = 9.12 KK230 pKa = 10.25 VQVQMNASQFHH241 pKa = 6.28 SGSLGVFLVPEE252 pKa = 4.42 FTPQNTFEE260 pKa = 4.5 AKK262 pKa = 8.79 STTFKK267 pKa = 10.95 SLPRR271 pKa = 11.84 DD272 pKa = 3.43 KK273 pKa = 10.97 YY274 pKa = 10.32 IPEE277 pKa = 3.76 QMFVYY282 pKa = 9.31 PHH284 pKa = 6.59 QILNCRR290 pKa = 11.84 TNTSVDD296 pKa = 3.03 IQVPYY301 pKa = 11.02 CNFVPSSFNEE311 pKa = 3.7 IHH313 pKa = 5.67 NTWTLVVMVLTPLDD327 pKa = 4.11 YY328 pKa = 10.78 STGAATDD335 pKa = 3.49 IAITTSITPLDD346 pKa = 3.51 VLYY349 pKa = 11.25 NGLRR353 pKa = 11.84 HH354 pKa = 5.93 LKK356 pKa = 10.49 EE357 pKa = 3.98 EE358 pKa = 5.11 GIPTLPEE365 pKa = 4.11 GSSYY369 pKa = 10.96 TFSTTNIDD377 pKa = 3.28 MAQPVYY383 pKa = 11.08 SKK385 pKa = 11.2 GFNPTQNYY393 pKa = 9.6 IPGKK397 pKa = 8.57 FTNLLQMSVTPTLMANQIGSEE418 pKa = 4.33 GEE420 pKa = 4.29 SKK422 pKa = 10.31 QVGYY426 pKa = 8.97 FMCTNQVPTLPLFVSDD442 pKa = 3.85 VTLAAPKK449 pKa = 9.89 MKK451 pKa = 8.46 TTLVSAISQYY461 pKa = 8.07 FTQYY465 pKa = 10.08 RR466 pKa = 11.84 GSIVMDD472 pKa = 3.73 LVFTGTAMCKK482 pKa = 9.93 GKK484 pKa = 10.25 FVICYY489 pKa = 7.03 TPPGAAQPQTRR500 pKa = 11.84 EE501 pKa = 3.6 KK502 pKa = 11.22 AMQGTYY508 pKa = 10.71 AVWDD512 pKa = 4.01 LGLNSSFKK520 pKa = 10.04 FTIPFISVSDD530 pKa = 3.86 YY531 pKa = 10.81 RR532 pKa = 11.84 WVDD535 pKa = 3.46 GAPSTSISIDD545 pKa = 2.58 GWFTIWQLTPITYY558 pKa = 7.92 PANNPNISCVLVFASAGTDD577 pKa = 3.5 FSYY580 pKa = 11.3 RR581 pKa = 11.84 NLTDD585 pKa = 2.84 IPMRR589 pKa = 11.84 SQGTEE594 pKa = 3.66 VGEE597 pKa = 4.63 SGDD600 pKa = 3.89 TGNSNATEE608 pKa = 4.23 NIIGTSISTEE618 pKa = 4.04 VSHH621 pKa = 8.0 SEE623 pKa = 3.09 IDD625 pKa = 4.17 FIFSRR630 pKa = 11.84 YY631 pKa = 9.3 FNVDD635 pKa = 3.04 TTRR638 pKa = 11.84 VTTYY642 pKa = 9.6 TGNDD646 pKa = 4.29 GITSITLSIYY656 pKa = 10.96 DD657 pKa = 4.1 FLQKK661 pKa = 9.2 PTAQALKK668 pKa = 10.24 RR669 pKa = 11.84 FLQIVTYY676 pKa = 9.55 FKK678 pKa = 11.23 SDD680 pKa = 3.55 LEE682 pKa = 4.25 IAITPLNTISTKK694 pKa = 10.15 MFVKK698 pKa = 9.91 WLPSGSVVDD707 pKa = 5.53 LSRR710 pKa = 11.84 EE711 pKa = 4.0 TLNGINTPRR720 pKa = 11.84 FIKK723 pKa = 9.4 NTSGAPLQIFNTDD736 pKa = 2.73 ITNVCTFRR744 pKa = 11.84 VPYY747 pKa = 9.68 TSVLGAIPITYY758 pKa = 9.85 NGYY761 pKa = 11.23 GDD763 pKa = 4.79 FSQTAYY769 pKa = 10.94 NIAPGADD776 pKa = 3.14 FGTLLFANTGSVGMQIMLSVRR797 pKa = 11.84 FVNMQCWIPRR807 pKa = 11.84 TLAPKK812 pKa = 10.29 ISVTNTDD819 pKa = 3.56 SNIVAYY825 pKa = 10.31 RR826 pKa = 11.84 SALTEE831 pKa = 4.13 EE832 pKa = 4.93 IVFQGPIDD840 pKa = 4.05 YY841 pKa = 7.53 PTGQQCSCLNFFLLQEE857 pKa = 4.27 NLEE860 pKa = 4.15 EE861 pKa = 4.37 AMEE864 pKa = 4.82 EE865 pKa = 3.98 ISVLCSCNPSTKK877 pKa = 10.61 NIINKK882 pKa = 9.61 EE883 pKa = 4.09 SLEE886 pKa = 3.81 AMYY889 pKa = 10.41 QVLSHH894 pKa = 6.14 MSCYY898 pKa = 9.58 VHH900 pKa = 5.86 YY901 pKa = 8.72 TWEE904 pKa = 4.4 DD905 pKa = 3.29 VGFDD909 pKa = 3.69 QLPSTSYY916 pKa = 10.43 SGCYY920 pKa = 9.35 CDD922 pKa = 6.08 RR923 pKa = 11.84 DD924 pKa = 3.84 DD925 pKa = 5.01 FNNVLEE931 pKa = 4.79 TIGHH935 pKa = 6.47 NIWDD939 pKa = 4.23 LKK941 pKa = 10.98 SKK943 pKa = 10.76 GVDD946 pKa = 3.34 TQILEE951 pKa = 4.29 VYY953 pKa = 10.26 RR954 pKa = 11.84 MYY956 pKa = 10.77 ISDD959 pKa = 4.16 VEE961 pKa = 4.84 CEE963 pKa = 4.13 NHH965 pKa = 7.35 WIRR968 pKa = 11.84 DD969 pKa = 3.53 LAADD973 pKa = 4.2 GDD975 pKa = 4.51 VEE977 pKa = 4.52 QNPGPASYY985 pKa = 11.59 NLFFSNTRR993 pKa = 11.84 FLVTKK998 pKa = 10.49 RR999 pKa = 11.84 MRR1001 pKa = 11.84 KK1002 pKa = 7.55 SRR1004 pKa = 11.84 KK1005 pKa = 8.72 EE1006 pKa = 3.28 IRR1008 pKa = 11.84 QQGFIDD1014 pKa = 3.92 WLTDD1018 pKa = 3.54 GAVTSVKK1025 pKa = 10.45 DD1026 pKa = 3.31 AAQNLTNVIQSTNKK1040 pKa = 9.13 IMNRR1044 pKa = 11.84 AFSCRR1049 pKa = 11.84 TIIKK1053 pKa = 10.08 IITDD1057 pKa = 4.54 FLTSGLILYY1066 pKa = 7.93 TCDD1069 pKa = 3.81 FNPTIAAVLAIKK1081 pKa = 10.33 HH1082 pKa = 5.65 GLDD1085 pKa = 3.25 MLVEE1089 pKa = 4.27 GSVFVLITEE1098 pKa = 4.15 QLKK1101 pKa = 10.87 KK1102 pKa = 10.64 LFKK1105 pKa = 10.02 TDD1107 pKa = 3.45 PPTVDD1112 pKa = 3.03 GGEE1115 pKa = 4.13 IEE1117 pKa = 4.25 QQGVLRR1123 pKa = 11.84 DD1124 pKa = 3.8 FNTVVNAAKK1133 pKa = 10.39 GIEE1136 pKa = 4.16 WFLKK1140 pKa = 9.63 TIQNLYY1146 pKa = 10.7 DD1147 pKa = 3.76 WLKK1150 pKa = 9.98 SWCQKK1155 pKa = 9.93 EE1156 pKa = 4.32 EE1157 pKa = 4.11 EE1158 pKa = 4.62 SCHH1161 pKa = 5.14 GRR1163 pKa = 11.84 YY1164 pKa = 10.16 AKK1166 pKa = 10.35 MMDD1169 pKa = 3.42 TLGVEE1174 pKa = 4.26 LQVADD1179 pKa = 3.77 NLISRR1184 pKa = 11.84 IHH1186 pKa = 6.75 EE1187 pKa = 4.2 STKK1190 pKa = 10.37 EE1191 pKa = 3.89 NLRR1194 pKa = 11.84 TTIGKK1199 pKa = 8.42 LTEE1202 pKa = 3.84 YY1203 pKa = 10.46 SIIAMEE1209 pKa = 4.14 VEE1211 pKa = 4.35 KK1212 pKa = 9.61 TQVASIIEE1220 pKa = 4.1 KK1221 pKa = 10.22 RR1222 pKa = 11.84 LTPIRR1227 pKa = 11.84 NYY1229 pKa = 10.57 YY1230 pKa = 9.02 DD1231 pKa = 3.16 QLYY1234 pKa = 10.76 GSGTIRR1240 pKa = 11.84 AEE1242 pKa = 3.97 PPVVLLRR1249 pKa = 11.84 GKK1251 pKa = 8.89 PGCGKK1256 pKa = 10.28 SVVSMLMSQAISKK1269 pKa = 10.03 IVTGSQSVYY1278 pKa = 10.2 TFPTSSTHH1286 pKa = 5.13 MDD1288 pKa = 3.76 GYY1290 pKa = 8.92 KK1291 pKa = 9.36 QQYY1294 pKa = 8.76 VMVMDD1299 pKa = 5.96 DD1300 pKa = 4.66 LGQNPDD1306 pKa = 3.63 GEE1308 pKa = 4.42 DD1309 pKa = 3.39 FKK1311 pKa = 11.24 IFCQLVSTAKK1321 pKa = 10.11 FVVPMASLMEE1331 pKa = 4.92 KK1332 pKa = 9.27 GTEE1335 pKa = 4.0 FNTGVIIATTNLPEE1349 pKa = 4.56 FKK1351 pKa = 9.78 PVTIVEE1357 pKa = 4.3 PGAVDD1362 pKa = 5.37 RR1363 pKa = 11.84 RR1364 pKa = 11.84 ITWKK1368 pKa = 8.56 FTPEE1372 pKa = 3.24 IRR1374 pKa = 11.84 KK1375 pKa = 9.22 EE1376 pKa = 3.73 FLKK1379 pKa = 10.8 DD1380 pKa = 3.15 GKK1382 pKa = 11.15 LNLEE1386 pKa = 4.39 LSLNDD1391 pKa = 4.79 SGNTPPKK1398 pKa = 10.61 GFTRR1402 pKa = 11.84 DD1403 pKa = 3.4 CPLINGKK1410 pKa = 9.35 ALTLNTPSGAKK1421 pKa = 10.08 YY1422 pKa = 10.39 SVAEE1426 pKa = 4.15 AVDD1429 pKa = 5.13 LIIGTLKK1436 pKa = 10.6 HH1437 pKa = 6.19 KK1438 pKa = 11.15 SNVAMDD1444 pKa = 4.09 LSGLVEE1450 pKa = 4.21 QGVLDD1455 pKa = 5.36 DD1456 pKa = 4.86 MKK1458 pKa = 11.21 LDD1460 pKa = 4.06 DD1461 pKa = 4.7 AAQEE1465 pKa = 4.3 EE1466 pKa = 4.55 ILKK1469 pKa = 10.37 ILMQNNNIISRR1480 pKa = 11.84 EE1481 pKa = 3.75 ASKK1484 pKa = 10.93 QIFTRR1489 pKa = 11.84 ADD1491 pKa = 3.51 HH1492 pKa = 6.89 IIEE1495 pKa = 4.28 IEE1497 pKa = 4.22 NKK1499 pKa = 7.39 NTQPIRR1505 pKa = 11.84 EE1506 pKa = 4.15 TIAWILAASGTFFTLILLYY1525 pKa = 9.71 RR1526 pKa = 11.84 TFFCSVKK1533 pKa = 10.18 QGQGPYY1539 pKa = 9.56 DD1540 pKa = 3.57 GKK1542 pKa = 10.37 IAKK1545 pKa = 8.95 PRR1547 pKa = 11.84 QFKK1550 pKa = 10.46 EE1551 pKa = 3.79 ILAQAPDD1558 pKa = 3.86 LEE1560 pKa = 4.61 LSVLRR1565 pKa = 11.84 NCVPLDD1571 pKa = 3.27 VDD1573 pKa = 4.01 IPNKK1577 pKa = 9.51 PKK1579 pKa = 10.28 MMPFTALGLFEE1590 pKa = 5.16 LTFATNRR1597 pKa = 11.84 HH1598 pKa = 5.97 AIEE1601 pKa = 4.34 NCTSFEE1607 pKa = 4.37 IQGHH1611 pKa = 4.65 TYY1613 pKa = 10.12 KK1614 pKa = 10.34 IEE1616 pKa = 4.3 DD1617 pKa = 3.6 VDD1619 pKa = 3.93 VKK1621 pKa = 10.51 MVSTQEE1627 pKa = 3.97 GKK1629 pKa = 8.78 TDD1631 pKa = 3.83 LAIVTLKK1638 pKa = 10.66 KK1639 pKa = 9.59 GTRR1642 pKa = 11.84 FRR1644 pKa = 11.84 NIMKK1648 pKa = 9.91 HH1649 pKa = 5.95 LLDD1652 pKa = 5.44 EE1653 pKa = 4.54 IVEE1656 pKa = 4.42 PTGHH1660 pKa = 5.76 VVGIVNSSLFPRR1672 pKa = 11.84 TLFKK1676 pKa = 11.1 GKK1678 pKa = 10.25 ALRR1681 pKa = 11.84 TARR1684 pKa = 11.84 KK1685 pKa = 7.79 ITASGKK1691 pKa = 8.68 QMYY1694 pKa = 10.34 NVFSYY1699 pKa = 10.83 DD1700 pKa = 3.22 CPTYY1704 pKa = 10.66 GGYY1707 pKa = 10.41 CGAPIIGQVGNEE1719 pKa = 3.86 KK1720 pKa = 10.39 KK1721 pKa = 10.47 ILGIHH1726 pKa = 6.32 CAGDD1730 pKa = 3.82 GTTGWATVITKK1741 pKa = 10.62 NIVKK1745 pKa = 10.22 KK1746 pKa = 10.61 IEE1748 pKa = 4.0 EE1749 pKa = 4.17 QGLKK1753 pKa = 10.52 VPIGEE1758 pKa = 4.55 ANPVCHH1764 pKa = 7.0 VMRR1767 pKa = 11.84 KK1768 pKa = 9.51 SKK1770 pKa = 10.01 ICPSGFSYY1778 pKa = 8.53 PTDD1781 pKa = 3.51 VEE1783 pKa = 4.19 PAILTQKK1790 pKa = 10.68 DD1791 pKa = 3.26 PRR1793 pKa = 11.84 LDD1795 pKa = 4.02 DD1796 pKa = 4.91 GVVLDD1801 pKa = 5.03 DD1802 pKa = 5.99 KK1803 pKa = 11.19 IFEE1806 pKa = 4.12 KK1807 pKa = 10.54 HH1808 pKa = 4.76 QNNMEE1813 pKa = 4.15 TLPPVFEE1820 pKa = 4.77 VAAKK1824 pKa = 9.57 MYY1826 pKa = 9.7 AKK1828 pKa = 9.95 QVFSIVGKK1836 pKa = 10.51 DD1837 pKa = 3.03 NGEE1840 pKa = 3.91 ITTTEE1845 pKa = 4.52 AINGYY1850 pKa = 8.14 KK1851 pKa = 9.2 TAEE1854 pKa = 4.18 KK1855 pKa = 9.37 MDD1857 pKa = 4.37 LSTSPGYY1864 pKa = 9.94 PYY1866 pKa = 11.45 VNMGLRR1872 pKa = 11.84 RR1873 pKa = 11.84 EE1874 pKa = 4.03 NMLDD1878 pKa = 3.67 CADD1881 pKa = 3.67 GVYY1884 pKa = 9.59 TPKK1887 pKa = 10.93 DD1888 pKa = 3.24 WFNKK1892 pKa = 10.12 NIIAVEE1898 pKa = 4.19 KK1899 pKa = 10.23 DD1900 pKa = 3.12 PKK1902 pKa = 10.71 DD1903 pKa = 3.28 ATFATFLKK1911 pKa = 10.92 DD1912 pKa = 3.44 EE1913 pKa = 4.74 LRR1915 pKa = 11.84 PIAKK1919 pKa = 9.78 ARR1921 pKa = 11.84 SGKK1924 pKa = 7.54 TRR1926 pKa = 11.84 IVDD1929 pKa = 3.83 ASPFCHH1935 pKa = 6.92 AIVGRR1940 pKa = 11.84 KK1941 pKa = 9.02 LLLRR1945 pKa = 11.84 FTEE1948 pKa = 4.41 KK1949 pKa = 10.77 FMVNNGTSVGSAIGTDD1965 pKa = 4.11 PDD1967 pKa = 4.22 CDD1969 pKa = 2.97 WTRR1972 pKa = 11.84 FYY1974 pKa = 11.37 HH1975 pKa = 5.93 EE1976 pKa = 5.13 LSNEE1980 pKa = 3.99 YY1981 pKa = 10.75 VFDD1984 pKa = 4.44 LDD1986 pKa = 3.9 YY1987 pKa = 11.55 SQFDD1991 pKa = 3.98 STHH1994 pKa = 5.48 PTAMFDD2000 pKa = 3.77 LVQKK2004 pKa = 10.8 HH2005 pKa = 5.9 FFNTEE2010 pKa = 3.19 NGFDD2014 pKa = 3.73 EE2015 pKa = 4.56 KK2016 pKa = 10.41 TGKK2019 pKa = 10.41 YY2020 pKa = 9.86 LDD2022 pKa = 3.83 SLSVSKK2028 pKa = 10.5 HH2029 pKa = 3.94 VYY2031 pKa = 9.88 GRR2033 pKa = 11.84 EE2034 pKa = 3.62 KK2035 pKa = 10.59 FLTIGGLPSGCSCTSMLNTVFNNIIIRR2062 pKa = 11.84 AAILSCYY2069 pKa = 10.14 EE2070 pKa = 3.86 GVDD2073 pKa = 3.06 WSDD2076 pKa = 3.79 FKK2078 pKa = 11.15 MLAYY2082 pKa = 10.3 GDD2084 pKa = 4.18 DD2085 pKa = 3.6 VVYY2088 pKa = 10.74 SSRR2091 pKa = 11.84 DD2092 pKa = 3.33 PILPGKK2098 pKa = 8.6 IANWLHH2104 pKa = 6.1 QNTTYY2109 pKa = 11.66 KK2110 pKa = 9.74 LTPANKK2116 pKa = 8.56 TNVFPTEE2123 pKa = 4.1 SKK2125 pKa = 10.61 IEE2127 pKa = 3.94 DD2128 pKa = 3.26 VTFLKK2133 pKa = 10.6 RR2134 pKa = 11.84 KK2135 pKa = 9.02 FVTDD2139 pKa = 4.06 GVLVRR2144 pKa = 11.84 PVISRR2149 pKa = 11.84 TNIEE2153 pKa = 5.34 NMLAWKK2159 pKa = 10.47 RR2160 pKa = 11.84 NGEE2163 pKa = 4.11 FGDD2166 pKa = 4.13 KK2167 pKa = 9.82 IRR2169 pKa = 11.84 SVAGLAFHH2177 pKa = 7.21 LGEE2180 pKa = 5.32 DD2181 pKa = 3.99 EE2182 pKa = 4.42 YY2183 pKa = 11.97 NDD2185 pKa = 2.87 IFLEE2189 pKa = 4.15 IEE2191 pKa = 3.89 NDD2193 pKa = 3.59 SQYY2196 pKa = 11.72 SKK2198 pKa = 11.03 YY2199 pKa = 9.82 IPHH2202 pKa = 6.06 YY2203 pKa = 10.21 HH2204 pKa = 6.89 LLDD2207 pKa = 3.99 FVWKK2211 pKa = 9.01 QKK2213 pKa = 10.84 NGIYY2217 pKa = 9.91 II2218 pKa = 3.98
Molecular weight: 247.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.328
IPC2_protein 5.397
IPC_protein 5.41
Toseland 5.55
ProMoST 5.652
Dawson 5.537
Bjellqvist 5.614
Wikipedia 5.474
Rodwell 5.474
Grimsley 5.575
Solomon 5.537
Lehninger 5.512
Nozaki 5.728
DTASelect 5.906
Thurlkill 5.728
EMBOSS 5.69
Sillero 5.804
Patrickios 3.846
IPC_peptide 5.55
IPC2_peptide 5.804
IPC2.peptide.svr19 5.782
Protein with the highest isoelectric point:
>tr|A0A0A7EQ87|A0A0A7EQ87_9PICO Capsid protein VP0 OS=Tortoise picornavirus OX=1578826 PE=4 SV=1
MM1 pKa = 6.93 EE2 pKa = 4.11 TTMEE6 pKa = 3.94 ISFDD10 pKa = 3.65 ASCPSSVCIFCLCQPCKK27 pKa = 10.37 CHH29 pKa = 7.53 LIHH32 pKa = 7.31 NEE34 pKa = 3.85 IYY36 pKa = 9.17 TICYY40 pKa = 7.65 FTDD43 pKa = 3.62 EE44 pKa = 4.63 EE45 pKa = 4.29 KK46 pKa = 10.81 KK47 pKa = 10.22 EE48 pKa = 3.92 YY49 pKa = 10.16 QIQGQGQSNARR60 pKa = 11.84 PNQQSNYY67 pKa = 8.42 GTITNNYY74 pKa = 9.05 YY75 pKa = 10.4 EE76 pKa = 4.21 NTYY79 pKa = 9.91 IASADD84 pKa = 3.45 MSTQANGNEE93 pKa = 3.93 GDD95 pKa = 3.82 IPDD98 pKa = 4.09 VPGIWSTLNGVVDD111 pKa = 4.29 TASAMAPLLLDD122 pKa = 3.33 QDD124 pKa = 4.32 TEE126 pKa = 4.32 EE127 pKa = 4.36 TTNLSDD133 pKa = 3.52 RR134 pKa = 11.84 VKK136 pKa = 10.38 TSIHH140 pKa = 6.35 GNTSVGTQSSVGTVIGYY157 pKa = 9.74 KK158 pKa = 9.69 KK159 pKa = 10.71 EE160 pKa = 3.92 KK161 pKa = 8.9 DD162 pKa = 3.45 TNPISSCADD171 pKa = 3.18 APTVASFAMEE181 pKa = 3.85 RR182 pKa = 11.84 AFTQTMGTWSKK193 pKa = 10.18 TNDD196 pKa = 2.98 VYY198 pKa = 11.34 QYY200 pKa = 11.09 IHH202 pKa = 6.74 ICLPSGIDD210 pKa = 3.43 DD211 pKa = 4.1 GGVFSGVLNRR221 pKa = 11.84 HH222 pKa = 5.46 YY223 pKa = 11.22 LMKK226 pKa = 10.35 CGYY229 pKa = 9.12 KK230 pKa = 10.25 VQVQMNASQFHH241 pKa = 6.28 SGSLGVFLVPEE252 pKa = 4.42 FTPQNTFEE260 pKa = 4.5 AKK262 pKa = 8.79 STTFKK267 pKa = 10.95 SLPRR271 pKa = 11.84 DD272 pKa = 3.43 KK273 pKa = 10.97 YY274 pKa = 10.32 IPEE277 pKa = 3.76 QMFVYY282 pKa = 9.31 PHH284 pKa = 6.59 QILNCRR290 pKa = 11.84 TNTSVDD296 pKa = 3.03 IQVPYY301 pKa = 11.02 CNFVPSSFNEE311 pKa = 3.7 IHH313 pKa = 5.67 NTWTLVVMVLTPLDD327 pKa = 4.11 YY328 pKa = 10.78 STGAATDD335 pKa = 3.49 IAITTSITPLDD346 pKa = 3.51 VLYY349 pKa = 11.25 NGLRR353 pKa = 11.84 HH354 pKa = 5.93 LKK356 pKa = 10.49 EE357 pKa = 3.98 EE358 pKa = 5.11 GIPTLPEE365 pKa = 4.11 GSSYY369 pKa = 10.96 TFSTTNIDD377 pKa = 3.28 MAQPVYY383 pKa = 11.08 SKK385 pKa = 11.2 GFNPTQNYY393 pKa = 9.6 IPGKK397 pKa = 8.57 FTNLLQMSVTPTLMANQIGSEE418 pKa = 4.33 GEE420 pKa = 4.29 SKK422 pKa = 10.31 QVGYY426 pKa = 8.97 FMCTNQVPTLPLFVSDD442 pKa = 3.85 VTLAAPKK449 pKa = 9.89 MKK451 pKa = 8.46 TTLVSAISQYY461 pKa = 8.07 FTQYY465 pKa = 10.08 RR466 pKa = 11.84 GSIVMDD472 pKa = 3.73 LVFTGTAMCKK482 pKa = 9.93 GKK484 pKa = 10.25 FVICYY489 pKa = 7.03 TPPGAAQPQTRR500 pKa = 11.84 EE501 pKa = 3.6 KK502 pKa = 11.22 AMQGTYY508 pKa = 10.71 AVWDD512 pKa = 4.01 LGLNSSFKK520 pKa = 10.04 FTIPFISVSDD530 pKa = 3.86 YY531 pKa = 10.81 RR532 pKa = 11.84 WVDD535 pKa = 3.46 GAPSTSISIDD545 pKa = 2.58 GWFTIWQLTPITYY558 pKa = 7.92 PANNPNISCVLVFASAGTDD577 pKa = 3.5 FSYY580 pKa = 11.3 RR581 pKa = 11.84 NLTDD585 pKa = 2.84 IPMRR589 pKa = 11.84 SQGTEE594 pKa = 3.66 VGEE597 pKa = 4.63 SGDD600 pKa = 3.89 TGNSNATEE608 pKa = 4.23 NIIGTSISTEE618 pKa = 4.04 VSHH621 pKa = 8.0 SEE623 pKa = 3.09 IDD625 pKa = 4.17 FIFSRR630 pKa = 11.84 YY631 pKa = 9.3 FNVDD635 pKa = 3.04 TTRR638 pKa = 11.84 VTTYY642 pKa = 9.6 TGNDD646 pKa = 4.29 GITSITLSIYY656 pKa = 10.96 DD657 pKa = 4.1 FLQKK661 pKa = 9.2 PTAQALKK668 pKa = 10.24 RR669 pKa = 11.84 FLQIVTYY676 pKa = 9.55 FKK678 pKa = 11.23 SDD680 pKa = 3.55 LEE682 pKa = 4.25 IAITPLNTISTKK694 pKa = 10.15 MFVKK698 pKa = 9.91 WLPSGSVVDD707 pKa = 5.53 LSRR710 pKa = 11.84 EE711 pKa = 4.0 TLNGINTPRR720 pKa = 11.84 FIKK723 pKa = 9.4 NTSGAPLQIFNTDD736 pKa = 2.73 ITNVCTFRR744 pKa = 11.84 VPYY747 pKa = 9.68 TSVLGAIPITYY758 pKa = 9.85 NGYY761 pKa = 11.23 GDD763 pKa = 4.79 FSQTAYY769 pKa = 10.94 NIAPGADD776 pKa = 3.14 FGTLLFANTGSVGMQIMLSVRR797 pKa = 11.84 FVNMQCWIPRR807 pKa = 11.84 TLAPKK812 pKa = 10.29 ISVTNTDD819 pKa = 3.56 SNIVAYY825 pKa = 10.31 RR826 pKa = 11.84 SALTEE831 pKa = 4.13 EE832 pKa = 4.93 IVFQGPIDD840 pKa = 4.05 YY841 pKa = 7.53 PTGQQCSCLNFFLLQEE857 pKa = 4.27 NLEE860 pKa = 4.15 EE861 pKa = 4.37 AMEE864 pKa = 4.82 EE865 pKa = 3.98 ISVLCSCNPSTKK877 pKa = 10.61 NIINKK882 pKa = 9.61 EE883 pKa = 4.09 SLEE886 pKa = 3.81 AMYY889 pKa = 10.41 QVLSHH894 pKa = 6.14 MSCYY898 pKa = 9.58 VHH900 pKa = 5.86 YY901 pKa = 8.72 TWEE904 pKa = 4.4 DD905 pKa = 3.29 VGFDD909 pKa = 3.69 QLPSTSYY916 pKa = 10.43 SGCYY920 pKa = 9.35 CDD922 pKa = 6.08 RR923 pKa = 11.84 DD924 pKa = 3.84 DD925 pKa = 5.01 FNNVLEE931 pKa = 4.79 TIGHH935 pKa = 6.47 NIWDD939 pKa = 4.23 LKK941 pKa = 10.98 SKK943 pKa = 10.76 GVDD946 pKa = 3.34 TQILEE951 pKa = 4.29 VYY953 pKa = 10.26 RR954 pKa = 11.84 MYY956 pKa = 10.77 ISDD959 pKa = 4.16 VEE961 pKa = 4.84 CEE963 pKa = 4.13 NHH965 pKa = 7.35 WIRR968 pKa = 11.84 DD969 pKa = 3.53 LAADD973 pKa = 4.2 GDD975 pKa = 4.51 VEE977 pKa = 4.52 QNPGPASYY985 pKa = 11.59 NLFFSNTRR993 pKa = 11.84 FLVTKK998 pKa = 10.49 RR999 pKa = 11.84 MRR1001 pKa = 11.84 KK1002 pKa = 7.55 SRR1004 pKa = 11.84 KK1005 pKa = 8.72 EE1006 pKa = 3.28 IRR1008 pKa = 11.84 QQGFIDD1014 pKa = 3.92 WLTDD1018 pKa = 3.54 GAVTSVKK1025 pKa = 10.45 DD1026 pKa = 3.31 AAQNLTNVIQSTNKK1040 pKa = 9.13 IMNRR1044 pKa = 11.84 AFSCRR1049 pKa = 11.84 TIIKK1053 pKa = 10.08 IITDD1057 pKa = 4.54 FLTSGLILYY1066 pKa = 7.93 TCDD1069 pKa = 3.81 FNPTIAAVLAIKK1081 pKa = 10.33 HH1082 pKa = 5.65 GLDD1085 pKa = 3.25 MLVEE1089 pKa = 4.27 GSVFVLITEE1098 pKa = 4.15 QLKK1101 pKa = 10.87 KK1102 pKa = 10.64 LFKK1105 pKa = 10.02 TDD1107 pKa = 3.45 PPTVDD1112 pKa = 3.03 GGEE1115 pKa = 4.13 IEE1117 pKa = 4.25 QQGVLRR1123 pKa = 11.84 DD1124 pKa = 3.8 FNTVVNAAKK1133 pKa = 10.39 GIEE1136 pKa = 4.16 WFLKK1140 pKa = 9.63 TIQNLYY1146 pKa = 10.7 DD1147 pKa = 3.76 WLKK1150 pKa = 9.98 SWCQKK1155 pKa = 9.93 EE1156 pKa = 4.32 EE1157 pKa = 4.11 EE1158 pKa = 4.62 SCHH1161 pKa = 5.14 GRR1163 pKa = 11.84 YY1164 pKa = 10.16 AKK1166 pKa = 10.35 MMDD1169 pKa = 3.42 TLGVEE1174 pKa = 4.26 LQVADD1179 pKa = 3.77 NLISRR1184 pKa = 11.84 IHH1186 pKa = 6.75 EE1187 pKa = 4.2 STKK1190 pKa = 10.37 EE1191 pKa = 3.89 NLRR1194 pKa = 11.84 TTIGKK1199 pKa = 8.42 LTEE1202 pKa = 3.84 YY1203 pKa = 10.46 SIIAMEE1209 pKa = 4.14 VEE1211 pKa = 4.35 KK1212 pKa = 9.61 TQVASIIEE1220 pKa = 4.1 KK1221 pKa = 10.22 RR1222 pKa = 11.84 LTPIRR1227 pKa = 11.84 NYY1229 pKa = 10.57 YY1230 pKa = 9.02 DD1231 pKa = 3.16 QLYY1234 pKa = 10.76 GSGTIRR1240 pKa = 11.84 AEE1242 pKa = 3.97 PPVVLLRR1249 pKa = 11.84 GKK1251 pKa = 8.89 PGCGKK1256 pKa = 10.28 SVVSMLMSQAISKK1269 pKa = 10.03 IVTGSQSVYY1278 pKa = 10.2 TFPTSSTHH1286 pKa = 5.13 MDD1288 pKa = 3.76 GYY1290 pKa = 8.92 KK1291 pKa = 9.36 QQYY1294 pKa = 8.76 VMVMDD1299 pKa = 5.96 DD1300 pKa = 4.66 LGQNPDD1306 pKa = 3.63 GEE1308 pKa = 4.42 DD1309 pKa = 3.39 FKK1311 pKa = 11.24 IFCQLVSTAKK1321 pKa = 10.11 FVVPMASLMEE1331 pKa = 4.92 KK1332 pKa = 9.27 GTEE1335 pKa = 4.0 FNTGVIIATTNLPEE1349 pKa = 4.56 FKK1351 pKa = 9.78 PVTIVEE1357 pKa = 4.3 PGAVDD1362 pKa = 5.37 RR1363 pKa = 11.84 RR1364 pKa = 11.84 ITWKK1368 pKa = 8.56 FTPEE1372 pKa = 3.24 IRR1374 pKa = 11.84 KK1375 pKa = 9.22 EE1376 pKa = 3.73 FLKK1379 pKa = 10.8 DD1380 pKa = 3.15 GKK1382 pKa = 11.15 LNLEE1386 pKa = 4.39 LSLNDD1391 pKa = 4.79 SGNTPPKK1398 pKa = 10.61 GFTRR1402 pKa = 11.84 DD1403 pKa = 3.4 CPLINGKK1410 pKa = 9.35 ALTLNTPSGAKK1421 pKa = 10.08 YY1422 pKa = 10.39 SVAEE1426 pKa = 4.15 AVDD1429 pKa = 5.13 LIIGTLKK1436 pKa = 10.6 HH1437 pKa = 6.19 KK1438 pKa = 11.15 SNVAMDD1444 pKa = 4.09 LSGLVEE1450 pKa = 4.21 QGVLDD1455 pKa = 5.36 DD1456 pKa = 4.86 MKK1458 pKa = 11.21 LDD1460 pKa = 4.06 DD1461 pKa = 4.7 AAQEE1465 pKa = 4.3 EE1466 pKa = 4.55 ILKK1469 pKa = 10.37 ILMQNNNIISRR1480 pKa = 11.84 EE1481 pKa = 3.75 ASKK1484 pKa = 10.93 QIFTRR1489 pKa = 11.84 ADD1491 pKa = 3.51 HH1492 pKa = 6.89 IIEE1495 pKa = 4.28 IEE1497 pKa = 4.22 NKK1499 pKa = 7.39 NTQPIRR1505 pKa = 11.84 EE1506 pKa = 4.15 TIAWILAASGTFFTLILLYY1525 pKa = 9.71 RR1526 pKa = 11.84 TFFCSVKK1533 pKa = 10.18 QGQGPYY1539 pKa = 9.56 DD1540 pKa = 3.57 GKK1542 pKa = 10.37 IAKK1545 pKa = 8.95 PRR1547 pKa = 11.84 QFKK1550 pKa = 10.46 EE1551 pKa = 3.79 ILAQAPDD1558 pKa = 3.86 LEE1560 pKa = 4.61 LSVLRR1565 pKa = 11.84 NCVPLDD1571 pKa = 3.27 VDD1573 pKa = 4.01 IPNKK1577 pKa = 9.51 PKK1579 pKa = 10.28 MMPFTALGLFEE1590 pKa = 5.16 LTFATNRR1597 pKa = 11.84 HH1598 pKa = 5.97 AIEE1601 pKa = 4.34 NCTSFEE1607 pKa = 4.37 IQGHH1611 pKa = 4.65 TYY1613 pKa = 10.12 KK1614 pKa = 10.34 IEE1616 pKa = 4.3 DD1617 pKa = 3.6 VDD1619 pKa = 3.93 VKK1621 pKa = 10.51 MVSTQEE1627 pKa = 3.97 GKK1629 pKa = 8.78 TDD1631 pKa = 3.83 LAIVTLKK1638 pKa = 10.66 KK1639 pKa = 9.59 GTRR1642 pKa = 11.84 FRR1644 pKa = 11.84 NIMKK1648 pKa = 9.91 HH1649 pKa = 5.95 LLDD1652 pKa = 5.44 EE1653 pKa = 4.54 IVEE1656 pKa = 4.42 PTGHH1660 pKa = 5.76 VVGIVNSSLFPRR1672 pKa = 11.84 TLFKK1676 pKa = 11.1 GKK1678 pKa = 10.25 ALRR1681 pKa = 11.84 TARR1684 pKa = 11.84 KK1685 pKa = 7.79 ITASGKK1691 pKa = 8.68 QMYY1694 pKa = 10.34 NVFSYY1699 pKa = 10.83 DD1700 pKa = 3.22 CPTYY1704 pKa = 10.66 GGYY1707 pKa = 10.41 CGAPIIGQVGNEE1719 pKa = 3.86 KK1720 pKa = 10.39 KK1721 pKa = 10.47 ILGIHH1726 pKa = 6.32 CAGDD1730 pKa = 3.82 GTTGWATVITKK1741 pKa = 10.62 NIVKK1745 pKa = 10.22 KK1746 pKa = 10.61 IEE1748 pKa = 4.0 EE1749 pKa = 4.17 QGLKK1753 pKa = 10.52 VPIGEE1758 pKa = 4.55 ANPVCHH1764 pKa = 7.0 VMRR1767 pKa = 11.84 KK1768 pKa = 9.51 SKK1770 pKa = 10.01 ICPSGFSYY1778 pKa = 8.53 PTDD1781 pKa = 3.51 VEE1783 pKa = 4.19 PAILTQKK1790 pKa = 10.68 DD1791 pKa = 3.26 PRR1793 pKa = 11.84 LDD1795 pKa = 4.02 DD1796 pKa = 4.91 GVVLDD1801 pKa = 5.03 DD1802 pKa = 5.99 KK1803 pKa = 11.19 IFEE1806 pKa = 4.12 KK1807 pKa = 10.54 HH1808 pKa = 4.76 QNNMEE1813 pKa = 4.15 TLPPVFEE1820 pKa = 4.77 VAAKK1824 pKa = 9.57 MYY1826 pKa = 9.7 AKK1828 pKa = 9.95 QVFSIVGKK1836 pKa = 10.51 DD1837 pKa = 3.03 NGEE1840 pKa = 3.91 ITTTEE1845 pKa = 4.52 AINGYY1850 pKa = 8.14 KK1851 pKa = 9.2 TAEE1854 pKa = 4.18 KK1855 pKa = 9.37 MDD1857 pKa = 4.37 LSTSPGYY1864 pKa = 9.94 PYY1866 pKa = 11.45 VNMGLRR1872 pKa = 11.84 RR1873 pKa = 11.84 EE1874 pKa = 4.03 NMLDD1878 pKa = 3.67 CADD1881 pKa = 3.67 GVYY1884 pKa = 9.59 TPKK1887 pKa = 10.93 DD1888 pKa = 3.24 WFNKK1892 pKa = 10.12 NIIAVEE1898 pKa = 4.19 KK1899 pKa = 10.23 DD1900 pKa = 3.12 PKK1902 pKa = 10.71 DD1903 pKa = 3.28 ATFATFLKK1911 pKa = 10.92 DD1912 pKa = 3.44 EE1913 pKa = 4.74 LRR1915 pKa = 11.84 PIAKK1919 pKa = 9.78 ARR1921 pKa = 11.84 SGKK1924 pKa = 7.54 TRR1926 pKa = 11.84 IVDD1929 pKa = 3.83 ASPFCHH1935 pKa = 6.92 AIVGRR1940 pKa = 11.84 KK1941 pKa = 9.02 LLLRR1945 pKa = 11.84 FTEE1948 pKa = 4.41 KK1949 pKa = 10.77 FMVNNGTSVGSAIGTDD1965 pKa = 4.11 PDD1967 pKa = 4.22 CDD1969 pKa = 2.97 WTRR1972 pKa = 11.84 FYY1974 pKa = 11.37 HH1975 pKa = 5.93 EE1976 pKa = 5.13 LSNEE1980 pKa = 3.99 YY1981 pKa = 10.75 VFDD1984 pKa = 4.44 LDD1986 pKa = 3.9 YY1987 pKa = 11.55 SQFDD1991 pKa = 3.98 STHH1994 pKa = 5.48 PTAMFDD2000 pKa = 3.77 LVQKK2004 pKa = 10.8 HH2005 pKa = 5.9 FFNTEE2010 pKa = 3.19 NGFDD2014 pKa = 3.73 EE2015 pKa = 4.56 KK2016 pKa = 10.41 TGKK2019 pKa = 10.41 YY2020 pKa = 9.86 LDD2022 pKa = 3.83 SLSVSKK2028 pKa = 10.5 HH2029 pKa = 3.94 VYY2031 pKa = 9.88 GRR2033 pKa = 11.84 EE2034 pKa = 3.62 KK2035 pKa = 10.59 FLTIGGLPSGCSCTSMLNTVFNNIIIRR2062 pKa = 11.84 AAILSCYY2069 pKa = 10.14 EE2070 pKa = 3.86 GVDD2073 pKa = 3.06 WSDD2076 pKa = 3.79 FKK2078 pKa = 11.15 MLAYY2082 pKa = 10.3 GDD2084 pKa = 4.18 DD2085 pKa = 3.6 VVYY2088 pKa = 10.74 SSRR2091 pKa = 11.84 DD2092 pKa = 3.33 PILPGKK2098 pKa = 8.6 IANWLHH2104 pKa = 6.1 QNTTYY2109 pKa = 11.66 KK2110 pKa = 9.74 LTPANKK2116 pKa = 8.56 TNVFPTEE2123 pKa = 4.1 SKK2125 pKa = 10.61 IEE2127 pKa = 3.94 DD2128 pKa = 3.26 VTFLKK2133 pKa = 10.6 RR2134 pKa = 11.84 KK2135 pKa = 9.02 FVTDD2139 pKa = 4.06 GVLVRR2144 pKa = 11.84 PVISRR2149 pKa = 11.84 TNIEE2153 pKa = 5.34 NMLAWKK2159 pKa = 10.47 RR2160 pKa = 11.84 NGEE2163 pKa = 4.11 FGDD2166 pKa = 4.13 KK2167 pKa = 9.82 IRR2169 pKa = 11.84 SVAGLAFHH2177 pKa = 7.21 LGEE2180 pKa = 5.32 DD2181 pKa = 3.99 EE2182 pKa = 4.42 YY2183 pKa = 11.97 NDD2185 pKa = 2.87 IFLEE2189 pKa = 4.15 IEE2191 pKa = 3.89 NDD2193 pKa = 3.59 SQYY2196 pKa = 11.72 SKK2198 pKa = 11.03 YY2199 pKa = 9.82 IPHH2202 pKa = 6.06 YY2203 pKa = 10.21 HH2204 pKa = 6.89 LLDD2207 pKa = 3.99 FVWKK2211 pKa = 9.01 QKK2213 pKa = 10.84 NGIYY2217 pKa = 9.91 II2218 pKa = 3.98
Molecular weight: 247.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.328
IPC2_protein 5.397
IPC_protein 5.41
Toseland 5.55
ProMoST 5.652
Dawson 5.537
Bjellqvist 5.614
Wikipedia 5.474
Rodwell 5.474
Grimsley 5.575
Solomon 5.537
Lehninger 5.512
Nozaki 5.728
DTASelect 5.906
Thurlkill 5.728
EMBOSS 5.69
Sillero 5.804
Patrickios 3.846
IPC_peptide 5.55
IPC2_peptide 5.804
IPC2.peptide.svr19 5.782
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
2218
2218
2218
2218.0
247.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.365 ± 0.0
2.119 ± 0.0
5.681 ± 0.0
5.185 ± 0.0
4.869 ± 0.0
6.537 ± 0.0
1.623 ± 0.0
7.439 ± 0.0
5.951 ± 0.0
7.394 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.66 ± 0.0
5.591 ± 0.0
4.734 ± 0.0
3.922 ± 0.0
3.291 ± 0.0
7.078 ± 0.0
8.882 ± 0.0
6.763 ± 0.0
1.127 ± 0.0
3.787 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here