Caprine arthritis encephalitis virus Ov496
Average proteome isoelectric point is 8.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0JIH3|C0JIH3_CAEV Rev protein OS=Caprine arthritis encephalitis virus Ov496 OX=621214 PE=4 SV=1
MM1 pKa = 8.17 DD2 pKa = 4.16 MGAKK6 pKa = 9.9 HH7 pKa = 5.43 MQRR10 pKa = 11.84 TGEE13 pKa = 4.25 GNWVEE18 pKa = 4.28 VKK20 pKa = 9.96 MEE22 pKa = 3.88 EE23 pKa = 4.24 QEE25 pKa = 4.47 RR26 pKa = 11.84 EE27 pKa = 4.21 EE28 pKa = 4.35 EE29 pKa = 4.21 PLLHH33 pKa = 6.36 TRR35 pKa = 11.84 QQGKK39 pKa = 9.67 YY40 pKa = 8.4 KK41 pKa = 9.98 PQVSEE46 pKa = 4.12 QILNGDD52 pKa = 3.76 TNTCLTYY59 pKa = 10.96 AWIFLWGIQLTLWILLLLQWPSMGCKK85 pKa = 10.18 AEE87 pKa = 4.88 DD88 pKa = 4.3 YY89 pKa = 8.67 ITLISDD95 pKa = 4.17 PYY97 pKa = 10.58 GFQPVRR103 pKa = 11.84 NVSGVPVTCVTKK115 pKa = 10.79 KK116 pKa = 9.06 FAKK119 pKa = 9.41 WGCQPIGAYY128 pKa = 8.56 PNPEE132 pKa = 3.21 IEE134 pKa = 4.19 YY135 pKa = 10.4 RR136 pKa = 11.84 NISSDD141 pKa = 2.93 ILKK144 pKa = 10.21 EE145 pKa = 4.27 VYY147 pKa = 10.14 QEE149 pKa = 3.69 DD150 pKa = 4.59 WPWNTYY156 pKa = 8.43 HH157 pKa = 6.52 WPLWQMEE164 pKa = 4.21 NVKK167 pKa = 9.81 QWLKK171 pKa = 11.05 EE172 pKa = 3.78 NEE174 pKa = 3.74 KK175 pKa = 10.78 DD176 pKa = 3.98 YY177 pKa = 11.22 KK178 pKa = 10.8 RR179 pKa = 11.84 KK180 pKa = 10.45 LNTTKK185 pKa = 10.93 EE186 pKa = 4.15 DD187 pKa = 3.84 LDD189 pKa = 3.73 EE190 pKa = 4.58 LLAGKK195 pKa = 9.81 IRR197 pKa = 11.84 GRR199 pKa = 11.84 FCVPYY204 pKa = 10.13 PFALLKK210 pKa = 9.28 CTEE213 pKa = 4.18 WCWYY217 pKa = 8.3 PAAIDD222 pKa = 4.31 DD223 pKa = 4.03 EE224 pKa = 4.76 SGRR227 pKa = 11.84 AKK229 pKa = 10.44 NIKK232 pKa = 9.63 INCTNARR239 pKa = 11.84 AVSCTEE245 pKa = 3.7 EE246 pKa = 4.04 MPLASIHH253 pKa = 5.62 RR254 pKa = 11.84 AYY256 pKa = 8.95 WGKK259 pKa = 10.3 RR260 pKa = 11.84 EE261 pKa = 3.98 RR262 pKa = 11.84 EE263 pKa = 3.99 SMQFMNIRR271 pKa = 11.84 ACQGSKK277 pKa = 10.19 LRR279 pKa = 11.84 CQRR282 pKa = 11.84 RR283 pKa = 11.84 PGGCMEE289 pKa = 5.78 GYY291 pKa = 9.02 PIPIGAEE298 pKa = 3.92 IIPEE302 pKa = 3.97 SMKK305 pKa = 10.57 YY306 pKa = 10.55 LRR308 pKa = 11.84 GKK310 pKa = 9.48 KK311 pKa = 8.91 SQYY314 pKa = 11.07 GGIKK318 pKa = 9.98 DD319 pKa = 3.82 RR320 pKa = 11.84 NGEE323 pKa = 4.28 LNLPLTVRR331 pKa = 11.84 VWVKK335 pKa = 10.19 LANNSAWINGAPPHH349 pKa = 5.93 WKK351 pKa = 10.21 DD352 pKa = 3.78 RR353 pKa = 11.84 IKK355 pKa = 10.81 GSKK358 pKa = 9.59 GINGTLWGEE367 pKa = 4.29 LKK369 pKa = 10.6 SIHH372 pKa = 6.56 HH373 pKa = 6.85 LGFAVSYY380 pKa = 10.31 DD381 pKa = 3.7 GKK383 pKa = 8.85 WCNFTKK389 pKa = 10.38 HH390 pKa = 5.64 IRR392 pKa = 11.84 IGQEE396 pKa = 3.45 VFPYY400 pKa = 9.92 HH401 pKa = 6.63 YY402 pKa = 9.95 KK403 pKa = 10.68 PSWNCSAAKK412 pKa = 6.77 WTQYY416 pKa = 7.78 PTWQVTRR423 pKa = 11.84 YY424 pKa = 9.86 LDD426 pKa = 3.71 MVEE429 pKa = 5.02 HH430 pKa = 5.5 MTGEE434 pKa = 4.43 CVQRR438 pKa = 11.84 PEE440 pKa = 3.89 RR441 pKa = 11.84 HH442 pKa = 6.27 NITVGNGTITGNCSVTNWDD461 pKa = 3.46 GCNCSRR467 pKa = 11.84 SGNHH471 pKa = 6.9 LYY473 pKa = 10.69 NSSSGGLLVIICRR486 pKa = 11.84 QNKK489 pKa = 8.54 TITGIMGTNINWTTMMGILKK509 pKa = 9.94 NCSGCGNATLDD520 pKa = 3.64 RR521 pKa = 11.84 TGTGALGNVKK531 pKa = 10.28 NEE533 pKa = 4.08 NCSLPHH539 pKa = 7.55 INEE542 pKa = 4.29 SNKK545 pKa = 7.61 WTCAPRR551 pKa = 11.84 WRR553 pKa = 11.84 GGMSDD558 pKa = 3.24 SLYY561 pKa = 10.52 IAGGKK566 pKa = 7.62 QFWTRR571 pKa = 11.84 VKK573 pKa = 10.49 AQYY576 pKa = 10.37 SCEE579 pKa = 4.29 SNLEE583 pKa = 4.05 EE584 pKa = 5.32 LDD586 pKa = 4.18 GMMHH590 pKa = 5.97 QQILLQKK597 pKa = 8.19 YY598 pKa = 6.7 QVIRR602 pKa = 11.84 VRR604 pKa = 11.84 AYY606 pKa = 7.72 TYY608 pKa = 11.42 GMIDD612 pKa = 3.54 MPSSHH617 pKa = 6.04 NQRR620 pKa = 11.84 STRR623 pKa = 11.84 RR624 pKa = 11.84 KK625 pKa = 9.06 RR626 pKa = 11.84 EE627 pKa = 3.6 LGHH630 pKa = 6.04 RR631 pKa = 11.84 RR632 pKa = 11.84 KK633 pKa = 10.09 KK634 pKa = 10.55 RR635 pKa = 11.84 GIGLVIMLVTMAIVAAAGASLGVANAIQQSYY666 pKa = 8.18 TKK668 pKa = 10.67 AAVQTLANATAAQQDD683 pKa = 4.19 VLEE686 pKa = 4.4 ATYY689 pKa = 11.78 AMVQHH694 pKa = 5.93 VAKK697 pKa = 10.35 GVRR700 pKa = 11.84 ILEE703 pKa = 4.04 ARR705 pKa = 11.84 VARR708 pKa = 11.84 VEE710 pKa = 4.57 AITDD714 pKa = 4.42 RR715 pKa = 11.84 IMLYY719 pKa = 10.18 QEE721 pKa = 4.25 LDD723 pKa = 3.1 CWHH726 pKa = 5.77 YY727 pKa = 10.7 HH728 pKa = 4.7 QYY730 pKa = 11.15 CVTSTRR736 pKa = 11.84 AEE738 pKa = 3.88 VAKK741 pKa = 10.3 YY742 pKa = 10.58 INWTRR747 pKa = 11.84 FKK749 pKa = 11.2 DD750 pKa = 3.51 NCTWQQWEE758 pKa = 4.66 RR759 pKa = 11.84 EE760 pKa = 4.31 LQGHH764 pKa = 6.37 DD765 pKa = 3.84 EE766 pKa = 4.32 NLTMLLRR773 pKa = 11.84 EE774 pKa = 4.5 SARR777 pKa = 11.84 MTQLAQEE784 pKa = 4.21 QARR787 pKa = 11.84 RR788 pKa = 11.84 IPDD791 pKa = 2.85 VWEE794 pKa = 4.19 ALQQVFDD801 pKa = 3.76 WSGWFSWLKK810 pKa = 9.6 YY811 pKa = 9.94 IPLIVIGILGCIIIRR826 pKa = 11.84 IMICVCQPLIQVYY839 pKa = 7.94 RR840 pKa = 11.84 TLSTPRR846 pKa = 11.84 YY847 pKa = 8.51 QKK849 pKa = 9.22 VTVIMEE855 pKa = 4.04 TRR857 pKa = 11.84 ADD859 pKa = 3.56 VGGEE863 pKa = 3.74 NAQDD867 pKa = 3.42 FGNGFEE873 pKa = 4.1 EE874 pKa = 5.02 LEE876 pKa = 4.36 ISRR879 pKa = 11.84 EE880 pKa = 4.03 PQKK883 pKa = 10.38 VTIKK887 pKa = 10.54 KK888 pKa = 8.57 AWSNAWEE895 pKa = 4.11 LWEE898 pKa = 3.92 TSPWEE903 pKa = 4.05 EE904 pKa = 3.25 RR905 pKa = 11.84 WKK907 pKa = 11.21 KK908 pKa = 10.56 NLLKK912 pKa = 10.42 PLILPLTMGIWINGLLGEE930 pKa = 4.06 RR931 pKa = 11.84 HH932 pKa = 5.71 KK933 pKa = 11.24 NKK935 pKa = 10.06 KK936 pKa = 9.39 EE937 pKa = 3.97 RR938 pKa = 11.84 VDD940 pKa = 3.57 CEE942 pKa = 3.84 TWEE945 pKa = 4.58 KK946 pKa = 11.38 GDD948 pKa = 3.53
Molecular weight: 109.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.836
IPC2_protein 8.039
IPC_protein 7.805
Toseland 7.424
ProMoST 8.434
Dawson 8.595
Bjellqvist 8.931
Wikipedia 8.521
Rodwell 8.639
Grimsley 7.439
Solomon 8.624
Lehninger 8.639
Nozaki 9.151
DTASelect 8.653
Thurlkill 8.726
EMBOSS 8.785
Sillero 9.033
Patrickios 4.304
IPC_peptide 8.624
IPC2_peptide 7.892
IPC2.peptide.svr19 8.08
Protein with the highest isoelectric point:
>tr|C0JIH2|C0JIH2_CAEV Env polyprotein OS=Caprine arthritis encephalitis virus Ov496 OX=621214 PE=4 SV=1
MM1 pKa = 7.63 NEE3 pKa = 3.87 QPPQGRR9 pKa = 11.84 EE10 pKa = 3.72 LHH12 pKa = 6.37 PEE14 pKa = 4.3 EE15 pKa = 4.35 IVMSIWEE22 pKa = 4.32 RR23 pKa = 11.84 EE24 pKa = 3.89 RR25 pKa = 11.84 EE26 pKa = 4.0 TWQWTSVRR34 pKa = 11.84 VPNEE38 pKa = 3.13 ILQRR42 pKa = 11.84 WLAMLKK48 pKa = 9.89 IGRR51 pKa = 11.84 NRR53 pKa = 11.84 GKK55 pKa = 10.4 VYY57 pKa = 11.03 KK58 pKa = 10.35 EE59 pKa = 3.52 MQKK62 pKa = 10.35 WMWRR66 pKa = 11.84 HH67 pKa = 5.34 PKK69 pKa = 10.42 APVIRR74 pKa = 11.84 PCGCRR79 pKa = 11.84 LCNPGWGTT87 pKa = 3.57
Molecular weight: 10.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.125
IPC2_protein 8.96
IPC_protein 9.077
Toseland 9.984
ProMoST 9.545
Dawson 10.101
Bjellqvist 9.809
Wikipedia 10.218
Rodwell 10.482
Grimsley 10.131
Solomon 10.189
Lehninger 10.175
Nozaki 10.116
DTASelect 9.736
Thurlkill 9.999
EMBOSS 10.365
Sillero 10.072
Patrickios 10.35
IPC_peptide 10.189
IPC2_peptide 8.858
IPC2.peptide.svr19 7.853
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2944
87
1100
490.7
56.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.74 ± 0.563
2.276 ± 0.584
3.668 ± 0.338
7.948 ± 0.538
2.106 ± 0.322
7.643 ± 0.438
2.276 ± 0.159
6.827 ± 1.275
8.016 ± 0.733
7.541 ± 0.286
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.057 ± 0.654
4.416 ± 0.616
4.688 ± 0.406
6.012 ± 0.589
6.42 ± 0.866
4.042 ± 0.315
5.265 ± 0.384
5.095 ± 0.25
3.906 ± 0.753
3.057 ± 0.421
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here