Desulfuromusa kysingii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Desulfuromonadaceae; Desulfuromusa

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3339 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3Y870|A0A1H3Y870_9DELT Tautomerase OS=Desulfuromusa kysingii OX=37625 GN=SAMN05660420_01110 PE=3 SV=1
MM1 pKa = 7.42KK2 pKa = 9.9RR3 pKa = 11.84QIIATFILLILLVGTISTNVWAKK26 pKa = 9.5TVTLSWDD33 pKa = 3.35ASPSNVSGYY42 pKa = 10.19KK43 pKa = 9.49IYY45 pKa = 11.05YY46 pKa = 7.52GTEE49 pKa = 3.92STGSWNGSGATEE61 pKa = 4.31GDD63 pKa = 3.75SPLLVGDD70 pKa = 3.47VLTYY74 pKa = 10.6VIHH77 pKa = 7.54DD78 pKa = 4.61LPDD81 pKa = 4.62DD82 pKa = 3.77MDD84 pKa = 4.45HH85 pKa = 6.39YY86 pKa = 11.1FAVTAYY92 pKa = 10.29DD93 pKa = 3.94DD94 pKa = 3.97SDD96 pKa = 4.12NEE98 pKa = 4.36STYY101 pKa = 11.61SNIVHH106 pKa = 6.2SPVISHH112 pKa = 6.73SNTPPVLAPIGDD124 pKa = 3.83YY125 pKa = 10.77SISEE129 pKa = 4.24GATLHH134 pKa = 6.48FNLTATDD141 pKa = 3.87ADD143 pKa = 4.45GDD145 pKa = 4.28PLTYY149 pKa = 9.56TASNLPNGAGIDD161 pKa = 3.52AATGDD166 pKa = 4.47FSWTPTFDD174 pKa = 2.78QSQIYY179 pKa = 10.11AVTFSVSDD187 pKa = 3.89GEE189 pKa = 4.67SVDD192 pKa = 3.75TEE194 pKa = 4.56TINITVADD202 pKa = 3.98VALNRR207 pKa = 11.84APEE210 pKa = 4.1LSAIGNKK217 pKa = 9.44AVSEE221 pKa = 4.5GEE223 pKa = 4.04TQYY226 pKa = 11.22IPVSATDD233 pKa = 3.7PDD235 pKa = 4.15GDD237 pKa = 3.74NLVYY241 pKa = 10.71SVTNLPSGATFDD253 pKa = 3.38AANRR257 pKa = 11.84IFTWPTNFDD266 pKa = 3.09QAGIYY271 pKa = 10.53SLTFYY276 pKa = 11.24VSDD279 pKa = 3.61GTLTDD284 pKa = 3.91SEE286 pKa = 4.67QISITINNVNRR297 pKa = 11.84PPVLNSVGTQTVGEE311 pKa = 4.3GEE313 pKa = 4.01RR314 pKa = 11.84LSFTVSGVDD323 pKa = 3.34SDD325 pKa = 5.38GDD327 pKa = 3.84ALDD330 pKa = 4.68YY331 pKa = 10.97SAQNLPSGATFDD343 pKa = 3.71PLQRR347 pKa = 11.84LFSWTPSFEE356 pKa = 4.38ASTNTRR362 pKa = 11.84VYY364 pKa = 9.94PVTFTVSDD372 pKa = 4.38GSAEE376 pKa = 4.08DD377 pKa = 4.12SEE379 pKa = 5.15TITLNVTNVNRR390 pKa = 11.84APVLDD395 pKa = 4.91AIGTQVITEE404 pKa = 4.2GDD406 pKa = 3.95SVNLVVNASDD416 pKa = 4.29PDD418 pKa = 4.21NNPLTYY424 pKa = 10.22SASGLPAGAVFTAEE438 pKa = 3.83TFSFNWIPGNEE449 pKa = 4.1QSGSYY454 pKa = 7.2QVKK457 pKa = 9.24FVVSDD462 pKa = 4.02GSLSDD467 pKa = 3.89SEE469 pKa = 4.77TVTFTVNNGNEE480 pKa = 4.13PPVFSAIGAQSVEE493 pKa = 4.45EE494 pKa = 3.99NSPLSFVVVASDD506 pKa = 4.0SNGDD510 pKa = 3.72SLSYY514 pKa = 10.47SVQGLPDD521 pKa = 3.47GAVFDD526 pKa = 4.06EE527 pKa = 4.39SQRR530 pKa = 11.84RR531 pKa = 11.84FSWTPDD537 pKa = 3.12YY538 pKa = 10.29TQAGSFTVVFKK549 pKa = 11.24ASDD552 pKa = 3.67GTLSDD557 pKa = 3.98AEE559 pKa = 4.44TVEE562 pKa = 4.13ITVTNNNRR570 pKa = 11.84SPVVSGNPNGSAMATARR587 pKa = 11.84YY588 pKa = 9.89SFTPVASDD596 pKa = 3.9PDD598 pKa = 4.13GDD600 pKa = 4.08PLIFSITNKK609 pKa = 10.0PSWADD614 pKa = 3.27FSTATGEE621 pKa = 4.47LSGTPTAAQVGTSSDD636 pKa = 3.07ILISVSDD643 pKa = 4.49SISTMSLSPFSIEE656 pKa = 3.38VLAYY660 pKa = 9.54VQQDD664 pKa = 3.14SDD666 pKa = 3.67GDD668 pKa = 4.31GILDD672 pKa = 4.16HH673 pKa = 7.35LDD675 pKa = 3.84AFPDD679 pKa = 4.91DD680 pKa = 3.73NSEE683 pKa = 3.97WLDD686 pKa = 3.47TDD688 pKa = 4.14GDD690 pKa = 4.19QIGNNSDD697 pKa = 4.81LDD699 pKa = 4.15DD700 pKa = 5.84DD701 pKa = 4.61NDD703 pKa = 4.64GIADD707 pKa = 3.69VRR709 pKa = 11.84DD710 pKa = 3.94GFPLDD715 pKa = 3.65STQSGWVISATAGTGGYY732 pKa = 7.55LTPEE736 pKa = 4.45GDD738 pKa = 3.47TAVLYY743 pKa = 10.74GGSQGYY749 pKa = 8.73QLTPMSGYY757 pKa = 10.15YY758 pKa = 9.63IHH760 pKa = 7.82DD761 pKa = 4.14LLVDD765 pKa = 3.72NVSVGTVDD773 pKa = 3.5RR774 pKa = 11.84YY775 pKa = 10.07EE776 pKa = 4.1FEE778 pKa = 5.16NIGAHH783 pKa = 4.8HH784 pKa = 7.71TITAVFATIPTGLSYY799 pKa = 11.4NPISSGLIGVEE810 pKa = 3.99RR811 pKa = 11.84VDD813 pKa = 5.85GGDD816 pKa = 3.6DD817 pKa = 4.16SNNLVDD823 pKa = 5.83NKK825 pKa = 10.25PKK827 pKa = 10.71QNLDD831 pKa = 3.35YY832 pKa = 10.71QFKK835 pKa = 10.33VVLRR839 pKa = 11.84DD840 pKa = 3.68SVPADD845 pKa = 3.27QRR847 pKa = 11.84RR848 pKa = 11.84VFLILDD854 pKa = 3.33QYY856 pKa = 10.31KK857 pKa = 9.85YY858 pKa = 11.23EE859 pKa = 4.13MEE861 pKa = 4.37FDD863 pKa = 3.82SGLLSSGADD872 pKa = 3.35YY873 pKa = 11.5VLTARR878 pKa = 11.84LGPAFSHH885 pKa = 6.18SFHH888 pKa = 6.96FSAEE892 pKa = 4.13DD893 pKa = 3.23SSGNHH898 pKa = 4.67IWRR901 pKa = 11.84YY902 pKa = 7.08PASGDD907 pKa = 3.36LPGPVVSLLNGKK919 pKa = 9.0NVVGLAAKK927 pKa = 10.04INAYY931 pKa = 10.5ALTATEE937 pKa = 4.98AFNDD941 pKa = 3.64SQIYY945 pKa = 9.22RR946 pKa = 11.84WVPQSEE952 pKa = 4.19ISGHH956 pKa = 5.89FEE958 pKa = 3.99LVDD961 pKa = 3.32IGAPIATGEE970 pKa = 4.42GYY972 pKa = 9.98VLKK975 pKa = 9.54NTRR978 pKa = 11.84QSSLPNFSLYY988 pKa = 11.16GDD990 pKa = 3.33ISEE993 pKa = 4.69ATHH996 pKa = 6.47EE997 pKa = 4.46FQVHH1001 pKa = 5.9SGWNLISNPYY1011 pKa = 9.02GGNVSLADD1019 pKa = 3.27IEE1021 pKa = 4.59IRR1023 pKa = 11.84LGDD1026 pKa = 4.37ADD1028 pKa = 4.02PVPWLTAVADD1038 pKa = 3.87NLVVDD1043 pKa = 4.98VIYY1046 pKa = 10.71SYY1048 pKa = 11.5LGADD1052 pKa = 3.12WDD1054 pKa = 4.13NGNEE1058 pKa = 3.99FASAAGSKK1066 pKa = 10.17SAILVPWVGYY1076 pKa = 9.03WIYY1079 pKa = 11.41VNPTDD1084 pKa = 4.91QEE1086 pKa = 3.88ASLIILKK1093 pKa = 9.53PLQDD1097 pKa = 3.35

Molecular weight: 116.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H4AKL5|A0A1H4AKL5_9DELT ATP synthase subunit delta OS=Desulfuromusa kysingii OX=37625 GN=atpH PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPSRR10 pKa = 11.84IKK12 pKa = 10.54RR13 pKa = 11.84KK14 pKa = 8.04RR15 pKa = 11.84THH17 pKa = 6.09GFRR20 pKa = 11.84KK21 pKa = 9.94KK22 pKa = 9.27MQTKK26 pKa = 10.13SGRR29 pKa = 11.84NTINNRR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 8.88SLTVSIPQKK50 pKa = 10.79

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3339

0

3339

1111176

15

5045

332.8

36.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.416 ± 0.047

1.183 ± 0.017

5.489 ± 0.05

6.237 ± 0.041

4.17 ± 0.035

7.392 ± 0.088

2.063 ± 0.023

6.751 ± 0.032

5.115 ± 0.044

11.004 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.024

3.784 ± 0.032

4.1 ± 0.031

4.461 ± 0.043

4.991 ± 0.041

6.268 ± 0.041

5.32 ± 0.036

6.879 ± 0.037

1.009 ± 0.016

2.898 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski