Cadophora sp. DSE1049
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22755 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V1BVC4|A0A2V1BVC4_9HELO Protein kinase domain-containing protein OS=Cadophora sp. DSE1049 OX=1485229 GN=DL98DRAFT_656798 PE=4 SV=1
MM1 pKa = 7.02 QLKK4 pKa = 10.04 NLLPAALVAGAAAQSLTDD22 pKa = 3.86 VLAANNDD29 pKa = 3.99 TLSSLTSLISADD41 pKa = 3.7 PALLEE46 pKa = 4.26 ALGGASNITILAPSNDD62 pKa = 3.18 ALAAFLNSTAGAAAATDD79 pKa = 4.1 PGAVAALITYY89 pKa = 9.36 HH90 pKa = 6.01 VLNGTYY96 pKa = 9.42 PASAFTNTSQFIPTLLSNSSYY117 pKa = 11.66 SNVTGGQVVEE127 pKa = 3.85 ARR129 pKa = 11.84 LRR131 pKa = 11.84 GEE133 pKa = 3.88 TASIFTGLLSASNVSVANVNFTGGVVHH160 pKa = 7.04 VIDD163 pKa = 4.41 SVLTVPQSASNTAGALNLTSLAGALTSADD192 pKa = 3.28 LVEE195 pKa = 4.56 TVDD198 pKa = 3.83 GLSDD202 pKa = 3.41 VTIFAPSNEE211 pKa = 3.86 AFQAIGSALPNLSTEE226 pKa = 4.28 ALSSILTYY234 pKa = 10.5 HH235 pKa = 6.04 VVQGTVGYY243 pKa = 10.67 SSILTNTTLEE253 pKa = 4.44 TVNGEE258 pKa = 4.24 SVTISIVNGTVYY270 pKa = 10.8 VNSAMVTIPDD280 pKa = 3.45 ILIAGGVVHH289 pKa = 7.07 VIDD292 pKa = 5.5 SVLNPNNTSAAPPTAGATTTTPAFTGASSATDD324 pKa = 3.36 VPFTSGVSASTTVTSVPTRR343 pKa = 11.84 DD344 pKa = 3.61 PEE346 pKa = 4.2 ATSTSTAGAAPLQTGMIGLGALFGGAALVMGNLL379 pKa = 3.8
Molecular weight: 37.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.859
IPC_protein 3.834
Toseland 3.617
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.541
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.164
Thurlkill 3.681
EMBOSS 3.77
Sillero 3.948
Patrickios 0.947
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.846
Protein with the highest isoelectric point:
>tr|A0A2V1C141|A0A2V1C141_9HELO Uncharacterized protein OS=Cadophora sp. DSE1049 OX=1485229 GN=DL98DRAFT_590024 PE=4 SV=1
MM1 pKa = 7.25 QSHH4 pKa = 5.45 KK5 pKa = 8.84 TFRR8 pKa = 11.84 TKK10 pKa = 10.69 VKK12 pKa = 10.16 LARR15 pKa = 11.84 AQKK18 pKa = 9.1 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.15 WRR45 pKa = 11.84 KK46 pKa = 7.38 TRR48 pKa = 11.84 IGII51 pKa = 4.0
Molecular weight: 6.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22755
0
22755
9288616
49
7844
408.2
45.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.109 ± 0.014
1.396 ± 0.007
5.461 ± 0.014
6.094 ± 0.017
3.968 ± 0.01
6.976 ± 0.017
2.298 ± 0.007
5.334 ± 0.01
5.033 ± 0.018
8.962 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.236 ± 0.006
3.826 ± 0.01
5.743 ± 0.016
3.805 ± 0.011
5.735 ± 0.014
8.324 ± 0.017
6.102 ± 0.012
6.123 ± 0.011
1.588 ± 0.006
2.888 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here