Pacific flying fox faeces associated circular DNA virus-1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTT7|A0A140CTT7_9VIRU Replication-associated protein OS=Pacific flying fox faeces associated circular DNA virus-1 OX=1796004 PE=4 SV=1
MM1 pKa = 7.21QLSRR5 pKa = 11.84WCFTNFNIPQDD16 pKa = 3.8LAAGCDD22 pKa = 3.65APLTLPSEE30 pKa = 4.25RR31 pKa = 11.84LKK33 pKa = 10.4YY34 pKa = 10.56ACYY37 pKa = 10.12QIEE40 pKa = 4.22KK41 pKa = 10.5CPDD44 pKa = 3.02TGRR47 pKa = 11.84LHH49 pKa = 6.12AQGYY53 pKa = 7.54LHH55 pKa = 7.26FKK57 pKa = 10.65RR58 pKa = 11.84SIKK61 pKa = 10.74LGGLKK66 pKa = 10.12KK67 pKa = 10.87LPGFEE72 pKa = 4.06AAHH75 pKa = 6.14FEE77 pKa = 4.21GAKK80 pKa = 10.31ADD82 pKa = 3.75LQANRR87 pKa = 11.84NYY89 pKa = 8.56CTKK92 pKa = 10.13EE93 pKa = 3.92DD94 pKa = 3.78SRR96 pKa = 11.84VAGPFEE102 pKa = 4.49IGSTEE107 pKa = 3.84GLGPGARR114 pKa = 11.84SDD116 pKa = 3.6LLAVKK121 pKa = 10.19RR122 pKa = 11.84RR123 pKa = 11.84LDD125 pKa = 3.51EE126 pKa = 4.57GVDD129 pKa = 3.73EE130 pKa = 5.1VGLADD135 pKa = 3.69EE136 pKa = 6.27FFGEE140 pKa = 4.61WVKK143 pKa = 10.83HH144 pKa = 4.28RR145 pKa = 11.84ASFNVYY151 pKa = 10.02RR152 pKa = 11.84GLKK155 pKa = 9.36VPARR159 pKa = 11.84GKK161 pKa = 9.0DD162 pKa = 3.41TQTPLYY168 pKa = 9.25VCVGASGLGKK178 pKa = 9.03STFVYY183 pKa = 10.64DD184 pKa = 3.69EE185 pKa = 4.4TEE187 pKa = 3.81GTVFQKK193 pKa = 11.27ALTAKK198 pKa = 8.91WWDD201 pKa = 3.86GFNGTQPVLFDD212 pKa = 3.93DD213 pKa = 4.35FRR215 pKa = 11.84GAFQFTEE222 pKa = 4.31LLRR225 pKa = 11.84LANIGAYY232 pKa = 7.81QVEE235 pKa = 4.69VKK237 pKa = 10.65GGSIQFNPKK246 pKa = 9.69EE247 pKa = 3.61IWITSNLRR255 pKa = 11.84PDD257 pKa = 2.87QWYY260 pKa = 10.02NPSTIKK266 pKa = 10.75DD267 pKa = 3.34ITPLTRR273 pKa = 11.84RR274 pKa = 11.84ISKK277 pKa = 9.8IYY279 pKa = 10.14HH280 pKa = 4.57FTAFKK285 pKa = 10.49EE286 pKa = 3.93MRR288 pKa = 11.84LFQSTEE294 pKa = 3.87NEE296 pKa = 4.58SAWDD300 pKa = 3.39QYY302 pKa = 11.53SRR304 pKa = 11.84SEE306 pKa = 4.14EE307 pKa = 3.84FSTLNFNGRR316 pKa = 11.84HH317 pKa = 5.43GEE319 pKa = 4.01ATSDD323 pKa = 4.01LEE325 pKa = 4.74LVYY328 pKa = 11.07

Molecular weight: 37.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTT7|A0A140CTT7_9VIRU Replication-associated protein OS=Pacific flying fox faeces associated circular DNA virus-1 OX=1796004 PE=4 SV=1
MM1 pKa = 7.42PWRR4 pKa = 11.84RR5 pKa = 11.84SWKK8 pKa = 9.47RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84STYY14 pKa = 9.7RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84TTRR20 pKa = 11.84RR21 pKa = 11.84YY22 pKa = 9.13GRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84PWRR29 pKa = 11.84RR30 pKa = 11.84TRR32 pKa = 11.84TRR34 pKa = 11.84SMMSRR39 pKa = 11.84SRR41 pKa = 11.84NLRR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84GTRR50 pKa = 11.84VFTRR54 pKa = 11.84TDD56 pKa = 2.57KK57 pKa = 10.94HH58 pKa = 6.71VYY60 pKa = 7.78STVYY64 pKa = 9.0TQGNSYY70 pKa = 8.56TAGSFAPKK78 pKa = 10.16LADD81 pKa = 3.37APGYY85 pKa = 9.58EE86 pKa = 4.45NLYY89 pKa = 10.19QLYY92 pKa = 9.78DD93 pKa = 3.12QARR96 pKa = 11.84IVKK99 pKa = 9.02FSIRR103 pKa = 11.84IIPSLKK109 pKa = 8.99SQVMVPTQQLPGEE122 pKa = 4.29YY123 pKa = 9.84SSIVQQVYY131 pKa = 9.85VYY133 pKa = 11.07NATNWEE139 pKa = 4.2GTPNDD144 pKa = 3.76PNVFLEE150 pKa = 4.07QSGCRR155 pKa = 11.84SFSPYY160 pKa = 9.03KK161 pKa = 10.22TILARR166 pKa = 11.84RR167 pKa = 11.84AARR170 pKa = 11.84TLEE173 pKa = 3.83KK174 pKa = 10.42STFQNSEE181 pKa = 3.96YY182 pKa = 10.1DD183 pKa = 3.52TLEE186 pKa = 3.98QNHH189 pKa = 5.38VRR191 pKa = 11.84MHH193 pKa = 6.1PWIDD197 pKa = 2.88VDD199 pKa = 4.0YY200 pKa = 11.53GGGALNCLPIVVGFPPNQNSSPSVSQSWKK229 pKa = 9.45VEE231 pKa = 3.6ITFTHH236 pKa = 4.55QWKK239 pKa = 10.63SKK241 pKa = 9.97LL242 pKa = 3.64

Molecular weight: 28.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

570

242

328

285.0

32.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.14 ± 1.283

1.404 ± 0.369

4.386 ± 0.954

5.439 ± 1.362

5.263 ± 1.25

7.193 ± 1.427

1.754 ± 0.065

3.684 ± 0.022

5.263 ± 0.722

7.544 ± 1.387

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.228 ± 0.535

4.386 ± 0.366

5.088 ± 0.709

4.912 ± 0.557

9.123 ± 2.619

7.544 ± 1.516

7.193 ± 0.684

5.439 ± 0.749

2.456 ± 0.279

4.561 ± 0.782
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski