Streptomyces phage Bmoc

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Samistivirus; unclassified Samistivirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 214 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3SYU4|A0A6M3SYU4_9CAUD MazG-like nucleotide pyrophosphohydrolase OS=Streptomyces phage Bmoc OX=2725629 GN=80 PE=4 SV=1
MM1 pKa = 7.65CMDD4 pKa = 4.95CGAPMGEE11 pKa = 3.86IHH13 pKa = 6.78TGKK16 pKa = 10.29CNPDD20 pKa = 3.1NRR22 pKa = 11.84EE23 pKa = 3.61GYY25 pKa = 9.77AWMVLFEE32 pKa = 4.36DD33 pKa = 4.05TFLEE37 pKa = 4.67FEE39 pKa = 4.53PAPDD43 pKa = 3.32AHH45 pKa = 7.74LDD47 pKa = 3.85DD48 pKa = 4.97MGDD51 pKa = 3.13

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3T0Q3|A0A6M3T0Q3_9CAUD Uncharacterized protein OS=Streptomyces phage Bmoc OX=2725629 GN=270 PE=4 SV=1
MM1 pKa = 7.07GTPTRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84SKK10 pKa = 10.62EE11 pKa = 3.85KK12 pKa = 11.07NPDD15 pKa = 3.28SWWQGAYY22 pKa = 10.37AGIPHH27 pKa = 7.52IIASYY32 pKa = 9.76EE33 pKa = 3.87FEE35 pKa = 4.93LNGDD39 pKa = 4.09SITPKK44 pKa = 10.92ALIKK48 pKa = 10.51FKK50 pKa = 9.55NTRR53 pKa = 11.84GSYY56 pKa = 10.27KK57 pKa = 9.91FRR59 pKa = 11.84CVATNTRR66 pKa = 11.84TGKK69 pKa = 7.83TWIDD73 pKa = 4.4CINADD78 pKa = 3.44TGEE81 pKa = 4.06WKK83 pKa = 10.52SFYY86 pKa = 10.48VEE88 pKa = 4.05KK89 pKa = 10.94LKK91 pKa = 11.33GLVKK95 pKa = 10.08PRR97 pKa = 11.84RR98 pKa = 11.84PRR100 pKa = 11.84RR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84VAA106 pKa = 3.2

Molecular weight: 12.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

214

0

214

34790

32

2110

162.6

18.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.132 ± 0.416

1.058 ± 0.118

6.522 ± 0.203

7.203 ± 0.361

3.8 ± 0.135

7.45 ± 0.229

1.906 ± 0.132

5.306 ± 0.152

6.447 ± 0.312

7.261 ± 0.192

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.831 ± 0.101

4.507 ± 0.136

3.728 ± 0.157

3.162 ± 0.198

5.812 ± 0.194

5.979 ± 0.254

5.999 ± 0.394

7.215 ± 0.243

1.909 ± 0.106

3.774 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski