Tomato ringspot virus (isolate raspberry) (ToRSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Picornavirales; Secoviridae; Comovirinae; Nepovirus; Tomato ringspot virus

Average proteome isoelectric point is 8.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|P29150|POL1_TORVR RNA1 polyprotein OS=Tomato ringspot virus (isolate raspberry) OX=12281 PE=1 SV=2
MM1 pKa = 7.82SSICFAGGNHH11 pKa = 6.35ARR13 pKa = 11.84LPSKK17 pKa = 9.97AAYY20 pKa = 9.38YY21 pKa = 10.03RR22 pKa = 11.84AISDD26 pKa = 3.72RR27 pKa = 11.84EE28 pKa = 3.68LDD30 pKa = 3.46RR31 pKa = 11.84EE32 pKa = 4.33GRR34 pKa = 11.84FPCGCLAQYY43 pKa = 8.04TVQAPPPAKK52 pKa = 8.89TQEE55 pKa = 4.26KK56 pKa = 10.24AVGRR60 pKa = 11.84SADD63 pKa = 3.68LQKK66 pKa = 11.61GNVAPLKK73 pKa = 10.21KK74 pKa = 9.92QRR76 pKa = 11.84CDD78 pKa = 3.23VVVAVSGPPPLEE90 pKa = 3.6LVYY93 pKa = 9.68PARR96 pKa = 11.84VGQHH100 pKa = 6.76RR101 pKa = 11.84LDD103 pKa = 3.84QPSKK107 pKa = 11.05GPLAVPSAKK116 pKa = 8.7QTSTAMEE123 pKa = 4.13VVLSAEE129 pKa = 4.31EE130 pKa = 3.91AAITAPWLLRR140 pKa = 11.84PCKK143 pKa = 10.49GEE145 pKa = 4.26APPPPPLTQRR155 pKa = 11.84QQFAALKK162 pKa = 10.2KK163 pKa = 8.76RR164 pKa = 11.84LAVKK168 pKa = 9.04GQQIIRR174 pKa = 11.84EE175 pKa = 4.33HH176 pKa = 5.49IRR178 pKa = 11.84ARR180 pKa = 11.84KK181 pKa = 6.82AAKK184 pKa = 9.45YY185 pKa = 10.06AAIAKK190 pKa = 9.09AKK192 pKa = 9.96KK193 pKa = 9.51AAALAAVKK201 pKa = 9.78AAQEE205 pKa = 4.14APRR208 pKa = 11.84LAAQKK213 pKa = 10.6AAISKK218 pKa = 9.86ILRR221 pKa = 11.84DD222 pKa = 3.53RR223 pKa = 11.84DD224 pKa = 3.75VAALPPPPPPSAARR238 pKa = 11.84LAAEE242 pKa = 4.84AEE244 pKa = 4.33LASKK248 pKa = 10.95AEE250 pKa = 3.98SLRR253 pKa = 11.84RR254 pKa = 11.84LKK256 pKa = 10.82AFKK259 pKa = 9.52TFSRR263 pKa = 11.84VRR265 pKa = 11.84PALNTSFPPPPPPPPARR282 pKa = 11.84SSEE285 pKa = 3.86LLAAFEE291 pKa = 4.31AAMNRR296 pKa = 11.84SQPVQGGFSLPTRR309 pKa = 11.84KK310 pKa = 9.55GVYY313 pKa = 8.8VAPTVQGVVRR323 pKa = 11.84AGLRR327 pKa = 11.84AQKK330 pKa = 10.79GFLNAVSTGIVAGARR345 pKa = 11.84ILKK348 pKa = 10.23SKK350 pKa = 8.46SQNWFRR356 pKa = 11.84RR357 pKa = 11.84SMGIAHH363 pKa = 7.34DD364 pKa = 4.13YY365 pKa = 11.5VEE367 pKa = 4.8GCMASTVLGCAGPVVQRR384 pKa = 11.84QEE386 pKa = 3.86ACSVVAAPPIVEE398 pKa = 4.14PVLWVPPLSEE408 pKa = 4.15YY409 pKa = 11.55ANDD412 pKa = 4.3FPKK415 pKa = 10.05LTCSTFTEE423 pKa = 4.19WQRR426 pKa = 11.84PRR428 pKa = 11.84KK429 pKa = 9.64QSIAISNLFRR439 pKa = 11.84KK440 pKa = 10.4LIDD443 pKa = 3.44RR444 pKa = 11.84ALLVSGVSLIASVLLFEE461 pKa = 4.46IAEE464 pKa = 4.2NFAVRR469 pKa = 11.84QAVCPVEE476 pKa = 4.3MPSCATSVSEE486 pKa = 4.49KK487 pKa = 10.92SLVSLDD493 pKa = 3.39EE494 pKa = 4.49GNFYY498 pKa = 10.45LRR500 pKa = 11.84KK501 pKa = 9.21YY502 pKa = 10.06LSPPPYY508 pKa = 9.57PFGRR512 pKa = 11.84EE513 pKa = 3.49SFYY516 pKa = 10.92FQARR520 pKa = 11.84PRR522 pKa = 11.84FIGPMPSMVRR532 pKa = 11.84AVPQIVQQPTMTEE545 pKa = 3.8EE546 pKa = 4.57LEE548 pKa = 4.3FEE550 pKa = 4.77VPSSWSSPLPLFANFKK566 pKa = 9.56VNRR569 pKa = 11.84GACFLQVLPQRR580 pKa = 11.84VVLPDD585 pKa = 3.11EE586 pKa = 5.1CMDD589 pKa = 4.17LLSLFEE595 pKa = 4.7DD596 pKa = 3.99QLPEE600 pKa = 4.36GPLPSFSWSSPLPLFANFKK619 pKa = 9.56VNRR622 pKa = 11.84GACFLQVLPQRR633 pKa = 11.84VVLPDD638 pKa = 3.11EE639 pKa = 5.1CMDD642 pKa = 4.17LLSLFEE648 pKa = 4.7DD649 pKa = 3.99QLPEE653 pKa = 4.36GPLPSFSWSSPLPLFASFKK672 pKa = 9.84VNRR675 pKa = 11.84GACFLQVLPARR686 pKa = 11.84KK687 pKa = 9.05VVSDD691 pKa = 3.58EE692 pKa = 4.31FMDD695 pKa = 3.57VLPFLFSPLVSHH707 pKa = 6.55QEE709 pKa = 4.07EE710 pKa = 4.42EE711 pKa = 4.7PEE713 pKa = 4.01MVPAVLEE720 pKa = 4.39AADD723 pKa = 3.81SVGDD727 pKa = 3.4ITEE730 pKa = 4.29AFFDD734 pKa = 4.24DD735 pKa = 4.93LEE737 pKa = 5.02CEE739 pKa = 4.29SFYY742 pKa = 11.33DD743 pKa = 3.71SYY745 pKa = 11.79SDD747 pKa = 3.7EE748 pKa = 5.24EE749 pKa = 3.94EE750 pKa = 4.76AEE752 pKa = 4.02WAEE755 pKa = 4.21VPRR758 pKa = 11.84CKK760 pKa = 10.21TMSEE764 pKa = 4.21LCASLTLAGDD774 pKa = 3.67AEE776 pKa = 4.74GLRR779 pKa = 11.84KK780 pKa = 9.71SHH782 pKa = 6.2GVFLKK787 pKa = 10.86RR788 pKa = 11.84LVTYY792 pKa = 8.82LQSFEE797 pKa = 4.3EE798 pKa = 4.29PLYY801 pKa = 10.68SSRR804 pKa = 11.84AFYY807 pKa = 10.32SVKK810 pKa = 10.09VKK812 pKa = 10.11PVYY815 pKa = 9.94RR816 pKa = 11.84PKK818 pKa = 10.67KK819 pKa = 9.36FEE821 pKa = 3.65GHH823 pKa = 6.63IDD825 pKa = 3.81CTCLDD830 pKa = 3.65GNMGEE835 pKa = 4.35WEE837 pKa = 3.89WRR839 pKa = 11.84EE840 pKa = 3.97SVDD843 pKa = 5.1AMWRR847 pKa = 11.84CPGRR851 pKa = 11.84LLNTKK856 pKa = 8.79RR857 pKa = 11.84TFTRR861 pKa = 11.84DD862 pKa = 2.52DD863 pKa = 3.59WEE865 pKa = 4.06RR866 pKa = 11.84VQYY869 pKa = 11.0LRR871 pKa = 11.84IGFNEE876 pKa = 3.16GRR878 pKa = 11.84YY879 pKa = 8.22RR880 pKa = 11.84RR881 pKa = 11.84NWRR884 pKa = 11.84VLNLEE889 pKa = 3.99EE890 pKa = 4.6MDD892 pKa = 4.51LSLHH896 pKa = 6.84EE897 pKa = 4.22YY898 pKa = 10.29PEE900 pKa = 4.36ISSAPVQSSLFSRR913 pKa = 11.84VVDD916 pKa = 4.81RR917 pKa = 11.84GATLASSIPFVTRR930 pKa = 11.84SNCQSSLGTPGLNVHH945 pKa = 6.87TIHH948 pKa = 6.77QEE950 pKa = 3.67APTTLRR956 pKa = 11.84APPFTGARR964 pKa = 11.84NVMGSSDD971 pKa = 3.82AGANAAPYY979 pKa = 9.94RR980 pKa = 11.84SEE982 pKa = 3.8ARR984 pKa = 11.84KK985 pKa = 9.18RR986 pKa = 11.84WLSRR990 pKa = 11.84KK991 pKa = 9.53QEE993 pKa = 4.19DD994 pKa = 3.79SQEE997 pKa = 4.15DD998 pKa = 3.8NIKK1001 pKa = 10.47RR1002 pKa = 11.84YY1003 pKa = 9.7ADD1005 pKa = 3.03KK1006 pKa = 10.99HH1007 pKa = 5.95GISFEE1012 pKa = 4.03EE1013 pKa = 3.83ARR1015 pKa = 11.84AVYY1018 pKa = 9.31KK1019 pKa = 10.6APKK1022 pKa = 9.66EE1023 pKa = 3.98GVPTQRR1029 pKa = 11.84SILPDD1034 pKa = 3.15VRR1036 pKa = 11.84DD1037 pKa = 3.83AYY1039 pKa = 10.18SARR1042 pKa = 11.84SAGARR1047 pKa = 11.84VRR1049 pKa = 11.84SLFGGSPTTRR1059 pKa = 11.84AQRR1062 pKa = 11.84TEE1064 pKa = 4.28DD1065 pKa = 3.67FVLTSPSAGDD1075 pKa = 3.26ASSFSFYY1082 pKa = 10.59FNPVSEE1088 pKa = 4.43QEE1090 pKa = 3.95MAEE1093 pKa = 4.01QEE1095 pKa = 4.19RR1096 pKa = 11.84GGNTMLSLDD1105 pKa = 3.72AVEE1108 pKa = 4.96VVIDD1112 pKa = 3.97PVGMPGDD1119 pKa = 4.09DD1120 pKa = 3.57TDD1122 pKa = 4.0LTVMVLWCQNSDD1134 pKa = 3.59DD1135 pKa = 3.8QRR1137 pKa = 11.84ALIGAMSTFVGNGLARR1153 pKa = 11.84AVFYY1157 pKa = 10.56PGLKK1161 pKa = 10.09LLYY1164 pKa = 9.7ANCRR1168 pKa = 11.84VRR1170 pKa = 11.84DD1171 pKa = 3.75GRR1173 pKa = 11.84VLKK1176 pKa = 10.85VIVSSTNSTLTHH1188 pKa = 7.02GLPQAQVSIGTLRR1201 pKa = 11.84QHH1203 pKa = 7.27LGPGHH1208 pKa = 7.21DD1209 pKa = 3.46RR1210 pKa = 11.84TISGALYY1217 pKa = 10.43ASQQQGFNIRR1227 pKa = 11.84ATEE1230 pKa = 3.74QGGAVTFAPQGGHH1243 pKa = 5.5VEE1245 pKa = 5.43GIPSANVQMGAGEE1258 pKa = 4.51HH1259 pKa = 6.96LIQAGPMQWRR1269 pKa = 11.84LQRR1272 pKa = 11.84SQSSRR1277 pKa = 11.84FVVSGHH1283 pKa = 5.03SRR1285 pKa = 11.84TRR1287 pKa = 11.84GSSLFTGSVDD1297 pKa = 3.09RR1298 pKa = 11.84TQQGTGAFEE1307 pKa = 4.95DD1308 pKa = 4.32PGFLPPRR1315 pKa = 11.84NSSVQGGSWQEE1326 pKa = 3.91GTEE1329 pKa = 3.68AAYY1332 pKa = 10.41LGKK1335 pKa = 8.75VTCAKK1340 pKa = 9.91DD1341 pKa = 3.55AKK1343 pKa = 10.72GGTLLHH1349 pKa = 6.25TLDD1352 pKa = 4.74IIKK1355 pKa = 10.08EE1356 pKa = 4.3CKK1358 pKa = 9.45SQNLLRR1364 pKa = 11.84YY1365 pKa = 9.8KK1366 pKa = 9.72EE1367 pKa = 3.99WQRR1370 pKa = 11.84QGFLHH1375 pKa = 6.3GKK1377 pKa = 9.37LRR1379 pKa = 11.84LRR1381 pKa = 11.84CFIPTNIFCGHH1392 pKa = 6.07SMMCSLDD1399 pKa = 3.09AFGRR1403 pKa = 11.84YY1404 pKa = 9.18DD1405 pKa = 4.0SNVLGASFPVKK1416 pKa = 10.17LASLLPTEE1424 pKa = 4.99VISLADD1430 pKa = 3.79GPVVTWTFDD1439 pKa = 2.64IGRR1442 pKa = 11.84LCGHH1446 pKa = 6.7GLYY1449 pKa = 10.46YY1450 pKa = 10.52SEE1452 pKa = 4.61GAYY1455 pKa = 10.07ARR1457 pKa = 11.84PKK1459 pKa = 10.22IYY1461 pKa = 10.49FLVLSDD1467 pKa = 3.78NDD1469 pKa = 3.68VPAEE1473 pKa = 4.12ADD1475 pKa = 3.31WQFTYY1480 pKa = 11.06QLLFEE1485 pKa = 5.03DD1486 pKa = 4.35HH1487 pKa = 6.51TFSNSFGAVPFITLPHH1503 pKa = 6.42IFNRR1507 pKa = 11.84LDD1509 pKa = 2.74IGYY1512 pKa = 8.64WRR1514 pKa = 11.84GPTEE1518 pKa = 4.14IDD1520 pKa = 3.51LTSTPAPNAYY1530 pKa = 9.83RR1531 pKa = 11.84LLFGLSTVISGNMSTLNANQALLRR1555 pKa = 11.84FFQGSNGTLHH1565 pKa = 6.84GRR1567 pKa = 11.84IKK1569 pKa = 10.84KK1570 pKa = 10.02IGTALTTCSLLLSLRR1585 pKa = 11.84HH1586 pKa = 6.18KK1587 pKa = 10.56DD1588 pKa = 3.21ASLTLEE1594 pKa = 3.93TAYY1597 pKa = 10.26QRR1599 pKa = 11.84PHH1601 pKa = 6.76YY1602 pKa = 10.14ILADD1606 pKa = 3.69GQGAFSLPISTPHH1619 pKa = 6.84AATSFLEE1626 pKa = 4.2DD1627 pKa = 3.47MLRR1630 pKa = 11.84LEE1632 pKa = 4.76IFAIAGPFSPKK1643 pKa = 10.17DD1644 pKa = 3.51NKK1646 pKa = 10.77AKK1648 pKa = 10.16YY1649 pKa = 10.39QFMCYY1654 pKa = 9.18FDD1656 pKa = 5.62HH1657 pKa = 7.27IEE1659 pKa = 4.0LVEE1662 pKa = 4.19GVPRR1666 pKa = 11.84TIAGEE1671 pKa = 4.07QQFNWCSFRR1680 pKa = 11.84NFKK1683 pKa = 10.23IDD1685 pKa = 3.13DD1686 pKa = 3.86WKK1688 pKa = 11.32FEE1690 pKa = 4.09WPARR1694 pKa = 11.84LPDD1697 pKa = 3.62ILDD1700 pKa = 4.21DD1701 pKa = 4.13KK1702 pKa = 11.73SEE1704 pKa = 4.07VLLRR1708 pKa = 11.84QHH1710 pKa = 6.82PLSLLISSTGFFTGRR1725 pKa = 11.84AIFVFQWGLNTTAGNMKK1742 pKa = 10.23GSFSARR1748 pKa = 11.84LAFGKK1753 pKa = 10.19GVEE1756 pKa = 4.6EE1757 pKa = 4.35IEE1759 pKa = 4.37QTSTVQPLVGACEE1772 pKa = 3.59ARR1774 pKa = 11.84IPVEE1778 pKa = 3.89FKK1780 pKa = 10.24TYY1782 pKa = 9.73TGYY1785 pKa = 7.24TTSGPPGSMEE1795 pKa = 3.68PYY1797 pKa = 9.31IYY1799 pKa = 10.89VRR1801 pKa = 11.84LTQAKK1806 pKa = 9.63LVDD1809 pKa = 3.75RR1810 pKa = 11.84LSVNVILQEE1819 pKa = 4.1GFSFYY1824 pKa = 11.01GPSVKK1829 pKa = 10.09HH1830 pKa = 5.62FKK1832 pKa = 10.83KK1833 pKa = 10.7EE1834 pKa = 3.76VGTPSATLGTNNPVGRR1850 pKa = 11.84PPEE1853 pKa = 4.2NVDD1856 pKa = 2.93TGGPGGQYY1864 pKa = 10.68AAALQAAQQAGKK1876 pKa = 10.31NPFGRR1881 pKa = 11.84GG1882 pKa = 3.08

Molecular weight:
206.8 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|P25247|POL2_TORVR RNA2 polyprotein OS=Tomato ringspot virus (isolate raspberry) OX=12281 PE=1 SV=1
MM1 pKa = 7.73PCLSAYY7 pKa = 11.18LLMLSSWEE15 pKa = 4.17SSCLRR20 pKa = 11.84LSQRR24 pKa = 11.84LRR26 pKa = 11.84ASEE29 pKa = 4.26RR30 pKa = 11.84YY31 pKa = 8.79GAAFAPASEE40 pKa = 4.6DD41 pKa = 4.26PITLRR46 pKa = 11.84APVKK50 pKa = 9.69GGARR54 pKa = 11.84NVVGASWCIVCTFKK68 pKa = 10.98PGVPRR73 pKa = 11.84EE74 pKa = 3.9DD75 pKa = 3.23WQLEE79 pKa = 4.15RR80 pKa = 11.84VTKK83 pKa = 10.71GILLAKK89 pKa = 9.35VAPLSTTLEE98 pKa = 3.99KK99 pKa = 10.97RR100 pKa = 11.84EE101 pKa = 4.24DD102 pKa = 3.49CTGADD107 pKa = 3.87EE108 pKa = 4.89ISGYY112 pKa = 7.84SCKK115 pKa = 10.01EE116 pKa = 3.45RR117 pKa = 11.84SISSRR122 pKa = 11.84FKK124 pKa = 8.27TRR126 pKa = 11.84QFRR129 pKa = 11.84RR130 pKa = 11.84YY131 pKa = 9.45LPSLKK136 pKa = 10.14PMRR139 pKa = 11.84KK140 pKa = 8.45YY141 pKa = 9.43CTRR144 pKa = 11.84SQSSRR149 pKa = 11.84VNVRR153 pKa = 11.84FVLSKK158 pKa = 10.84RR159 pKa = 11.84PGQRR163 pKa = 11.84HH164 pKa = 4.88IASTLSRR171 pKa = 11.84HH172 pKa = 4.75SHH174 pKa = 5.54SPILPSRR181 pKa = 11.84QVQSICPSNFLGRR194 pKa = 3.77

Molecular weight:
21.81 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

4474

194

2197

1118.5

123.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.857 ± 2.225

2.079 ± 0.529

4.291 ± 0.758

5.342 ± 0.295

4.649 ± 0.393

7.242 ± 1.0

1.833 ± 0.384

4.113 ± 0.554

4.94 ± 0.519

9.723 ± 0.126

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.168 ± 0.263

3.084 ± 0.33

6.102 ± 0.947

4.113 ± 0.601

6.705 ± 0.903

8.136 ± 0.672

4.381 ± 0.298

6.996 ± 0.799

1.252 ± 0.083

2.995 ± 0.543

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski