Tomato ringspot virus (isolate raspberry) (ToRSV)
Average proteome isoelectric point is 8.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P29150|POL1_TORVR RNA1 polyprotein OS=Tomato ringspot virus (isolate raspberry) OX=12281 PE=1 SV=2
MM1 pKa = 7.82 SSICFAGGNHH11 pKa = 6.35 ARR13 pKa = 11.84 LPSKK17 pKa = 9.97 AAYY20 pKa = 9.38 YY21 pKa = 10.03 RR22 pKa = 11.84 AISDD26 pKa = 3.72 RR27 pKa = 11.84 EE28 pKa = 3.68 LDD30 pKa = 3.46 RR31 pKa = 11.84 EE32 pKa = 4.33 GRR34 pKa = 11.84 FPCGCLAQYY43 pKa = 8.04 TVQAPPPAKK52 pKa = 8.89 TQEE55 pKa = 4.26 KK56 pKa = 10.24 AVGRR60 pKa = 11.84 SADD63 pKa = 3.68 LQKK66 pKa = 11.61 GNVAPLKK73 pKa = 10.21 KK74 pKa = 9.92 QRR76 pKa = 11.84 CDD78 pKa = 3.23 VVVAVSGPPPLEE90 pKa = 3.6 LVYY93 pKa = 9.68 PARR96 pKa = 11.84 VGQHH100 pKa = 6.76 RR101 pKa = 11.84 LDD103 pKa = 3.84 QPSKK107 pKa = 11.05 GPLAVPSAKK116 pKa = 8.7 QTSTAMEE123 pKa = 4.13 VVLSAEE129 pKa = 4.31 EE130 pKa = 3.91 AAITAPWLLRR140 pKa = 11.84 PCKK143 pKa = 10.49 GEE145 pKa = 4.26 APPPPPLTQRR155 pKa = 11.84 QQFAALKK162 pKa = 10.2 KK163 pKa = 8.76 RR164 pKa = 11.84 LAVKK168 pKa = 9.04 GQQIIRR174 pKa = 11.84 EE175 pKa = 4.33 HH176 pKa = 5.49 IRR178 pKa = 11.84 ARR180 pKa = 11.84 KK181 pKa = 6.82 AAKK184 pKa = 9.45 YY185 pKa = 10.06 AAIAKK190 pKa = 9.09 AKK192 pKa = 9.96 KK193 pKa = 9.51 AAALAAVKK201 pKa = 9.78 AAQEE205 pKa = 4.14 APRR208 pKa = 11.84 LAAQKK213 pKa = 10.6 AAISKK218 pKa = 9.86 ILRR221 pKa = 11.84 DD222 pKa = 3.53 RR223 pKa = 11.84 DD224 pKa = 3.75 VAALPPPPPPSAARR238 pKa = 11.84 LAAEE242 pKa = 4.84 AEE244 pKa = 4.33 LASKK248 pKa = 10.95 AEE250 pKa = 3.98 SLRR253 pKa = 11.84 RR254 pKa = 11.84 LKK256 pKa = 10.82 AFKK259 pKa = 9.52 TFSRR263 pKa = 11.84 VRR265 pKa = 11.84 PALNTSFPPPPPPPPARR282 pKa = 11.84 SSEE285 pKa = 3.86 LLAAFEE291 pKa = 4.31 AAMNRR296 pKa = 11.84 SQPVQGGFSLPTRR309 pKa = 11.84 KK310 pKa = 9.55 GVYY313 pKa = 8.8 VAPTVQGVVRR323 pKa = 11.84 AGLRR327 pKa = 11.84 AQKK330 pKa = 10.79 GFLNAVSTGIVAGARR345 pKa = 11.84 ILKK348 pKa = 10.23 SKK350 pKa = 8.46 SQNWFRR356 pKa = 11.84 RR357 pKa = 11.84 SMGIAHH363 pKa = 7.34 DD364 pKa = 4.13 YY365 pKa = 11.5 VEE367 pKa = 4.8 GCMASTVLGCAGPVVQRR384 pKa = 11.84 QEE386 pKa = 3.86 ACSVVAAPPIVEE398 pKa = 4.14 PVLWVPPLSEE408 pKa = 4.15 YY409 pKa = 11.55 ANDD412 pKa = 4.3 FPKK415 pKa = 10.05 LTCSTFTEE423 pKa = 4.19 WQRR426 pKa = 11.84 PRR428 pKa = 11.84 KK429 pKa = 9.64 QSIAISNLFRR439 pKa = 11.84 KK440 pKa = 10.4 LIDD443 pKa = 3.44 RR444 pKa = 11.84 ALLVSGVSLIASVLLFEE461 pKa = 4.46 IAEE464 pKa = 4.2 NFAVRR469 pKa = 11.84 QAVCPVEE476 pKa = 4.3 MPSCATSVSEE486 pKa = 4.49 KK487 pKa = 10.92 SLVSLDD493 pKa = 3.39 EE494 pKa = 4.49 GNFYY498 pKa = 10.45 LRR500 pKa = 11.84 KK501 pKa = 9.21 YY502 pKa = 10.06 LSPPPYY508 pKa = 9.57 PFGRR512 pKa = 11.84 EE513 pKa = 3.49 SFYY516 pKa = 10.92 FQARR520 pKa = 11.84 PRR522 pKa = 11.84 FIGPMPSMVRR532 pKa = 11.84 AVPQIVQQPTMTEE545 pKa = 3.8 EE546 pKa = 4.57 LEE548 pKa = 4.3 FEE550 pKa = 4.77 VPSSWSSPLPLFANFKK566 pKa = 9.56 VNRR569 pKa = 11.84 GACFLQVLPQRR580 pKa = 11.84 VVLPDD585 pKa = 3.11 EE586 pKa = 5.1 CMDD589 pKa = 4.17 LLSLFEE595 pKa = 4.7 DD596 pKa = 3.99 QLPEE600 pKa = 4.36 GPLPSFSWSSPLPLFANFKK619 pKa = 9.56 VNRR622 pKa = 11.84 GACFLQVLPQRR633 pKa = 11.84 VVLPDD638 pKa = 3.11 EE639 pKa = 5.1 CMDD642 pKa = 4.17 LLSLFEE648 pKa = 4.7 DD649 pKa = 3.99 QLPEE653 pKa = 4.36 GPLPSFSWSSPLPLFASFKK672 pKa = 9.84 VNRR675 pKa = 11.84 GACFLQVLPARR686 pKa = 11.84 KK687 pKa = 9.05 VVSDD691 pKa = 3.58 EE692 pKa = 4.31 FMDD695 pKa = 3.57 VLPFLFSPLVSHH707 pKa = 6.55 QEE709 pKa = 4.07 EE710 pKa = 4.42 EE711 pKa = 4.7 PEE713 pKa = 4.01 MVPAVLEE720 pKa = 4.39 AADD723 pKa = 3.81 SVGDD727 pKa = 3.4 ITEE730 pKa = 4.29 AFFDD734 pKa = 4.24 DD735 pKa = 4.93 LEE737 pKa = 5.02 CEE739 pKa = 4.29 SFYY742 pKa = 11.33 DD743 pKa = 3.71 SYY745 pKa = 11.79 SDD747 pKa = 3.7 EE748 pKa = 5.24 EE749 pKa = 3.94 EE750 pKa = 4.76 AEE752 pKa = 4.02 WAEE755 pKa = 4.21 VPRR758 pKa = 11.84 CKK760 pKa = 10.21 TMSEE764 pKa = 4.21 LCASLTLAGDD774 pKa = 3.67 AEE776 pKa = 4.74 GLRR779 pKa = 11.84 KK780 pKa = 9.71 SHH782 pKa = 6.2 GVFLKK787 pKa = 10.86 RR788 pKa = 11.84 LVTYY792 pKa = 8.82 LQSFEE797 pKa = 4.3 EE798 pKa = 4.29 PLYY801 pKa = 10.68 SSRR804 pKa = 11.84 AFYY807 pKa = 10.32 SVKK810 pKa = 10.09 VKK812 pKa = 10.11 PVYY815 pKa = 9.94 RR816 pKa = 11.84 PKK818 pKa = 10.67 KK819 pKa = 9.36 FEE821 pKa = 3.65 GHH823 pKa = 6.63 IDD825 pKa = 3.81 CTCLDD830 pKa = 3.65 GNMGEE835 pKa = 4.35 WEE837 pKa = 3.89 WRR839 pKa = 11.84 EE840 pKa = 3.97 SVDD843 pKa = 5.1 AMWRR847 pKa = 11.84 CPGRR851 pKa = 11.84 LLNTKK856 pKa = 8.79 RR857 pKa = 11.84 TFTRR861 pKa = 11.84 DD862 pKa = 2.52 DD863 pKa = 3.59 WEE865 pKa = 4.06 RR866 pKa = 11.84 VQYY869 pKa = 11.0 LRR871 pKa = 11.84 IGFNEE876 pKa = 3.16 GRR878 pKa = 11.84 YY879 pKa = 8.22 RR880 pKa = 11.84 RR881 pKa = 11.84 NWRR884 pKa = 11.84 VLNLEE889 pKa = 3.99 EE890 pKa = 4.6 MDD892 pKa = 4.51 LSLHH896 pKa = 6.84 EE897 pKa = 4.22 YY898 pKa = 10.29 PEE900 pKa = 4.36 ISSAPVQSSLFSRR913 pKa = 11.84 VVDD916 pKa = 4.81 RR917 pKa = 11.84 GATLASSIPFVTRR930 pKa = 11.84 SNCQSSLGTPGLNVHH945 pKa = 6.87 TIHH948 pKa = 6.77 QEE950 pKa = 3.67 APTTLRR956 pKa = 11.84 APPFTGARR964 pKa = 11.84 NVMGSSDD971 pKa = 3.82 AGANAAPYY979 pKa = 9.94 RR980 pKa = 11.84 SEE982 pKa = 3.8 ARR984 pKa = 11.84 KK985 pKa = 9.18 RR986 pKa = 11.84 WLSRR990 pKa = 11.84 KK991 pKa = 9.53 QEE993 pKa = 4.19 DD994 pKa = 3.79 SQEE997 pKa = 4.15 DD998 pKa = 3.8 NIKK1001 pKa = 10.47 RR1002 pKa = 11.84 YY1003 pKa = 9.7 ADD1005 pKa = 3.03 KK1006 pKa = 10.99 HH1007 pKa = 5.95 GISFEE1012 pKa = 4.03 EE1013 pKa = 3.83 ARR1015 pKa = 11.84 AVYY1018 pKa = 9.31 KK1019 pKa = 10.6 APKK1022 pKa = 9.66 EE1023 pKa = 3.98 GVPTQRR1029 pKa = 11.84 SILPDD1034 pKa = 3.15 VRR1036 pKa = 11.84 DD1037 pKa = 3.83 AYY1039 pKa = 10.18 SARR1042 pKa = 11.84 SAGARR1047 pKa = 11.84 VRR1049 pKa = 11.84 SLFGGSPTTRR1059 pKa = 11.84 AQRR1062 pKa = 11.84 TEE1064 pKa = 4.28 DD1065 pKa = 3.67 FVLTSPSAGDD1075 pKa = 3.26 ASSFSFYY1082 pKa = 10.59 FNPVSEE1088 pKa = 4.43 QEE1090 pKa = 3.95 MAEE1093 pKa = 4.01 QEE1095 pKa = 4.19 RR1096 pKa = 11.84 GGNTMLSLDD1105 pKa = 3.72 AVEE1108 pKa = 4.96 VVIDD1112 pKa = 3.97 PVGMPGDD1119 pKa = 4.09 DD1120 pKa = 3.57 TDD1122 pKa = 4.0 LTVMVLWCQNSDD1134 pKa = 3.59 DD1135 pKa = 3.8 QRR1137 pKa = 11.84 ALIGAMSTFVGNGLARR1153 pKa = 11.84 AVFYY1157 pKa = 10.56 PGLKK1161 pKa = 10.09 LLYY1164 pKa = 9.7 ANCRR1168 pKa = 11.84 VRR1170 pKa = 11.84 DD1171 pKa = 3.75 GRR1173 pKa = 11.84 VLKK1176 pKa = 10.85 VIVSSTNSTLTHH1188 pKa = 7.02 GLPQAQVSIGTLRR1201 pKa = 11.84 QHH1203 pKa = 7.27 LGPGHH1208 pKa = 7.21 DD1209 pKa = 3.46 RR1210 pKa = 11.84 TISGALYY1217 pKa = 10.43 ASQQQGFNIRR1227 pKa = 11.84 ATEE1230 pKa = 3.74 QGGAVTFAPQGGHH1243 pKa = 5.5 VEE1245 pKa = 5.43 GIPSANVQMGAGEE1258 pKa = 4.51 HH1259 pKa = 6.96 LIQAGPMQWRR1269 pKa = 11.84 LQRR1272 pKa = 11.84 SQSSRR1277 pKa = 11.84 FVVSGHH1283 pKa = 5.03 SRR1285 pKa = 11.84 TRR1287 pKa = 11.84 GSSLFTGSVDD1297 pKa = 3.09 RR1298 pKa = 11.84 TQQGTGAFEE1307 pKa = 4.95 DD1308 pKa = 4.32 PGFLPPRR1315 pKa = 11.84 NSSVQGGSWQEE1326 pKa = 3.91 GTEE1329 pKa = 3.68 AAYY1332 pKa = 10.41 LGKK1335 pKa = 8.75 VTCAKK1340 pKa = 9.91 DD1341 pKa = 3.55 AKK1343 pKa = 10.72 GGTLLHH1349 pKa = 6.25 TLDD1352 pKa = 4.74 IIKK1355 pKa = 10.08 EE1356 pKa = 4.3 CKK1358 pKa = 9.45 SQNLLRR1364 pKa = 11.84 YY1365 pKa = 9.8 KK1366 pKa = 9.72 EE1367 pKa = 3.99 WQRR1370 pKa = 11.84 QGFLHH1375 pKa = 6.3 GKK1377 pKa = 9.37 LRR1379 pKa = 11.84 LRR1381 pKa = 11.84 CFIPTNIFCGHH1392 pKa = 6.07 SMMCSLDD1399 pKa = 3.09 AFGRR1403 pKa = 11.84 YY1404 pKa = 9.18 DD1405 pKa = 4.0 SNVLGASFPVKK1416 pKa = 10.17 LASLLPTEE1424 pKa = 4.99 VISLADD1430 pKa = 3.79 GPVVTWTFDD1439 pKa = 2.64 IGRR1442 pKa = 11.84 LCGHH1446 pKa = 6.7 GLYY1449 pKa = 10.46 YY1450 pKa = 10.52 SEE1452 pKa = 4.61 GAYY1455 pKa = 10.07 ARR1457 pKa = 11.84 PKK1459 pKa = 10.22 IYY1461 pKa = 10.49 FLVLSDD1467 pKa = 3.78 NDD1469 pKa = 3.68 VPAEE1473 pKa = 4.12 ADD1475 pKa = 3.31 WQFTYY1480 pKa = 11.06 QLLFEE1485 pKa = 5.03 DD1486 pKa = 4.35 HH1487 pKa = 6.51 TFSNSFGAVPFITLPHH1503 pKa = 6.42 IFNRR1507 pKa = 11.84 LDD1509 pKa = 2.74 IGYY1512 pKa = 8.64 WRR1514 pKa = 11.84 GPTEE1518 pKa = 4.14 IDD1520 pKa = 3.51 LTSTPAPNAYY1530 pKa = 9.83 RR1531 pKa = 11.84 LLFGLSTVISGNMSTLNANQALLRR1555 pKa = 11.84 FFQGSNGTLHH1565 pKa = 6.84 GRR1567 pKa = 11.84 IKK1569 pKa = 10.84 KK1570 pKa = 10.02 IGTALTTCSLLLSLRR1585 pKa = 11.84 HH1586 pKa = 6.18 KK1587 pKa = 10.56 DD1588 pKa = 3.21 ASLTLEE1594 pKa = 3.93 TAYY1597 pKa = 10.26 QRR1599 pKa = 11.84 PHH1601 pKa = 6.76 YY1602 pKa = 10.14 ILADD1606 pKa = 3.69 GQGAFSLPISTPHH1619 pKa = 6.84 AATSFLEE1626 pKa = 4.2 DD1627 pKa = 3.47 MLRR1630 pKa = 11.84 LEE1632 pKa = 4.76 IFAIAGPFSPKK1643 pKa = 10.17 DD1644 pKa = 3.51 NKK1646 pKa = 10.77 AKK1648 pKa = 10.16 YY1649 pKa = 10.39 QFMCYY1654 pKa = 9.18 FDD1656 pKa = 5.62 HH1657 pKa = 7.27 IEE1659 pKa = 4.0 LVEE1662 pKa = 4.19 GVPRR1666 pKa = 11.84 TIAGEE1671 pKa = 4.07 QQFNWCSFRR1680 pKa = 11.84 NFKK1683 pKa = 10.23 IDD1685 pKa = 3.13 DD1686 pKa = 3.86 WKK1688 pKa = 11.32 FEE1690 pKa = 4.09 WPARR1694 pKa = 11.84 LPDD1697 pKa = 3.62 ILDD1700 pKa = 4.21 DD1701 pKa = 4.13 KK1702 pKa = 11.73 SEE1704 pKa = 4.07 VLLRR1708 pKa = 11.84 QHH1710 pKa = 6.82 PLSLLISSTGFFTGRR1725 pKa = 11.84 AIFVFQWGLNTTAGNMKK1742 pKa = 10.23 GSFSARR1748 pKa = 11.84 LAFGKK1753 pKa = 10.19 GVEE1756 pKa = 4.6 EE1757 pKa = 4.35 IEE1759 pKa = 4.37 QTSTVQPLVGACEE1772 pKa = 3.59 ARR1774 pKa = 11.84 IPVEE1778 pKa = 3.89 FKK1780 pKa = 10.24 TYY1782 pKa = 9.73 TGYY1785 pKa = 7.24 TTSGPPGSMEE1795 pKa = 3.68 PYY1797 pKa = 9.31 IYY1799 pKa = 10.89 VRR1801 pKa = 11.84 LTQAKK1806 pKa = 9.63 LVDD1809 pKa = 3.75 RR1810 pKa = 11.84 LSVNVILQEE1819 pKa = 4.1 GFSFYY1824 pKa = 11.01 GPSVKK1829 pKa = 10.09 HH1830 pKa = 5.62 FKK1832 pKa = 10.83 KK1833 pKa = 10.7 EE1834 pKa = 3.76 VGTPSATLGTNNPVGRR1850 pKa = 11.84 PPEE1853 pKa = 4.2 NVDD1856 pKa = 2.93 TGGPGGQYY1864 pKa = 10.68 AAALQAAQQAGKK1876 pKa = 10.31 NPFGRR1881 pKa = 11.84 GG1882 pKa = 3.08
Molecular weight: 206.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.387
IPC2_protein 7.775
IPC_protein 7.541
Toseland 7.146
ProMoST 8.185
Dawson 8.287
Bjellqvist 8.712
Wikipedia 8.214
Rodwell 8.331
Grimsley 7.19
Solomon 8.317
Lehninger 8.331
Nozaki 8.916
DTASelect 8.404
Thurlkill 8.463
EMBOSS 8.492
Sillero 8.799
Patrickios 4.457
IPC_peptide 8.317
IPC2_peptide 7.658
IPC2.peptide.svr19 7.804
Protein with the highest isoelectric point:
>sp|P25247|POL2_TORVR RNA2 polyprotein OS=Tomato ringspot virus (isolate raspberry) OX=12281 PE=1 SV=1
MM1 pKa = 7.73 PCLSAYY7 pKa = 11.18 LLMLSSWEE15 pKa = 4.17 SSCLRR20 pKa = 11.84 LSQRR24 pKa = 11.84 LRR26 pKa = 11.84 ASEE29 pKa = 4.26 RR30 pKa = 11.84 YY31 pKa = 8.79 GAAFAPASEE40 pKa = 4.6 DD41 pKa = 4.26 PITLRR46 pKa = 11.84 APVKK50 pKa = 9.69 GGARR54 pKa = 11.84 NVVGASWCIVCTFKK68 pKa = 10.98 PGVPRR73 pKa = 11.84 EE74 pKa = 3.9 DD75 pKa = 3.23 WQLEE79 pKa = 4.15 RR80 pKa = 11.84 VTKK83 pKa = 10.71 GILLAKK89 pKa = 9.35 VAPLSTTLEE98 pKa = 3.99 KK99 pKa = 10.97 RR100 pKa = 11.84 EE101 pKa = 4.24 DD102 pKa = 3.49 CTGADD107 pKa = 3.87 EE108 pKa = 4.89 ISGYY112 pKa = 7.84 SCKK115 pKa = 10.01 EE116 pKa = 3.45 RR117 pKa = 11.84 SISSRR122 pKa = 11.84 FKK124 pKa = 8.27 TRR126 pKa = 11.84 QFRR129 pKa = 11.84 RR130 pKa = 11.84 YY131 pKa = 9.45 LPSLKK136 pKa = 10.14 PMRR139 pKa = 11.84 KK140 pKa = 8.45 YY141 pKa = 9.43 CTRR144 pKa = 11.84 SQSSRR149 pKa = 11.84 VNVRR153 pKa = 11.84 FVLSKK158 pKa = 10.84 RR159 pKa = 11.84 PGQRR163 pKa = 11.84 HH164 pKa = 4.88 IASTLSRR171 pKa = 11.84 HH172 pKa = 4.75 SHH174 pKa = 5.54 SPILPSRR181 pKa = 11.84 QVQSICPSNFLGRR194 pKa = 3.77
Molecular weight: 21.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.358
IPC2_protein 9.575
IPC_protein 10.277
Toseland 10.57
ProMoST 10.379
Dawson 10.687
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.906
Grimsley 10.73
Solomon 10.774
Lehninger 10.745
Nozaki 10.599
DTASelect 10.379
Thurlkill 10.584
EMBOSS 10.965
Sillero 10.613
Patrickios 10.613
IPC_peptide 10.774
IPC2_peptide 9.692
IPC2.peptide.svr19 8.568
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
4474
194
2197
1118.5
123.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.857 ± 2.225
2.079 ± 0.529
4.291 ± 0.758
5.342 ± 0.295
4.649 ± 0.393
7.242 ± 1.0
1.833 ± 0.384
4.113 ± 0.554
4.94 ± 0.519
9.723 ± 0.126
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.168 ± 0.263
3.084 ± 0.33
6.102 ± 0.947
4.113 ± 0.601
6.705 ± 0.903
8.136 ± 0.672
4.381 ± 0.298
6.996 ± 0.799
1.252 ± 0.083
2.995 ± 0.543
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here