Amino acid dipepetide frequency for Firmicutes bacterium CAG:129

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.791AlaAla: 13.791 ± 0.261	1.58AlaCys: 1.58 ± 0.061	6.27AlaAsp: 6.27 ± 0.134	7.884AlaGlu: 7.884 ± 0.154	3.688AlaPhe: 3.688 ± 0.101	7.701AlaGly: 7.701 ± 0.149	1.869AlaHis: 1.869 ± 0.069	4.998AlaIle: 4.998 ± 0.119	4.62AlaLys: 4.62 ± 0.105	11.868AlaLeu: 11.868 ± 0.203	3.193AlaMet: 3.193 ± 0.088	2.594AlaAsn: 2.594 ± 0.084	3.579AlaPro: 3.579 ± 0.099	3.653AlaGln: 3.653 ± 0.099	5.408AlaArg: 5.408 ± 0.138	4.75AlaSer: 4.75 ± 0.093	3.825AlaThr: 3.825 ± 0.094	9.097AlaVal: 9.097 ± 0.156	0.88AlaTrp: 0.88 ± 0.049	3.102AlaTyr: 3.102 ± 0.087	0.004AlaXaa: 0.004 ± 0.003
Cys	1.999CysAla: 1.999 ± 0.073	0.353CysCys: 0.353 ± 0.03	0.977CysAsp: 0.977 ± 0.046	1.111CysGlu: 1.111 ± 0.05	0.651CysPhe: 0.651 ± 0.04	1.896CysGly: 1.896 ± 0.069	0.351CysHis: 0.351 ± 0.027	0.966CysIle: 0.966 ± 0.041	0.572CysLys: 0.572 ± 0.044	1.338CysLeu: 1.338 ± 0.053	0.392CysMet: 0.392 ± 0.031	0.494CysAsn: 0.494 ± 0.033	0.84CysPro: 0.84 ± 0.045	0.306CysGln: 0.306 ± 0.027	1.125CysArg: 1.125 ± 0.052	0.828CysSer: 0.828 ± 0.036	0.869CysThr: 0.869 ± 0.041	1.2CysVal: 1.2 ± 0.054	0.153CysTrp: 0.153 ± 0.019	0.543CysTyr: 0.543 ± 0.035	0.004CysXaa: 0.004 ± 0.003
Asp	6.47AspAla: 6.47 ± 0.135	0.909AspCys: 0.909 ± 0.044	2.835AspAsp: 2.835 ± 0.079	4.566AspGlu: 4.566 ± 0.106	2.331AspPhe: 2.331 ± 0.072	5.485AspGly: 5.485 ± 0.113	1.051AspHis: 1.051 ± 0.052	3.275AspIle: 3.275 ± 0.093	2.54AspLys: 2.54 ± 0.073	5.14AspLeu: 5.14 ± 0.103	1.621AspMet: 1.621 ± 0.058	1.489AspAsn: 1.489 ± 0.057	2.342AspPro: 2.342 ± 0.066	1.045AspGln: 1.045 ± 0.043	3.147AspArg: 3.147 ± 0.088	2.389AspSer: 2.389 ± 0.07	2.992AspThr: 2.992 ± 0.078	3.727AspVal: 3.727 ± 0.09	0.628AspTrp: 0.628 ± 0.045	2.236AspTyr: 2.236 ± 0.072	0.002AspXaa: 0.002 ± 0.002
Glu	6.044GluAla: 6.044 ± 0.111	0.894GluCys: 0.894 ± 0.046	3.568GluAsp: 3.568 ± 0.092	5.377GluGlu: 5.377 ± 0.136	2.096GluPhe: 2.096 ± 0.063	4.593GluGly: 4.593 ± 0.104	1.483GluHis: 1.483 ± 0.074	3.794GluIle: 3.794 ± 0.089	4.644GluLys: 4.644 ± 0.108	7.713GluLeu: 7.713 ± 0.133	2.059GluMet: 2.059 ± 0.069	3.112GluAsn: 3.112 ± 0.094	2.373GluPro: 2.373 ± 0.079	2.856GluGln: 2.856 ± 0.08	5.059GluArg: 5.059 ± 0.108	3.063GluSer: 3.063 ± 0.077	3.998GluThr: 3.998 ± 0.099	4.283GluVal: 4.283 ± 0.103	0.572GluTrp: 0.572 ± 0.039	2.098GluTyr: 2.098 ± 0.076	0.002GluXaa: 0.002 ± 0.002
Phe	3.915PheAla: 3.915 ± 0.098	0.822PheCys: 0.822 ± 0.045	2.559PheAsp: 2.559 ± 0.068	2.115PheGlu: 2.115 ± 0.069	1.592PhePhe: 1.592 ± 0.068	3.281PheGly: 3.281 ± 0.091	0.7PheHis: 0.7 ± 0.043	1.923PheIle: 1.923 ± 0.069	1.216PheLys: 1.216 ± 0.054	3.758PheLeu: 3.758 ± 0.097	0.871PheMet: 0.871 ± 0.047	1.086PheAsn: 1.086 ± 0.049	1.417PhePro: 1.417 ± 0.051	0.865PheGln: 0.865 ± 0.041	2.024PheArg: 2.024 ± 0.061	2.41PheSer: 2.41 ± 0.082	2.449PheThr: 2.449 ± 0.069	2.534PheVal: 2.534 ± 0.079	0.378PheTrp: 0.378 ± 0.029	1.41PheTyr: 1.41 ± 0.058	0.002PheXaa: 0.002 ± 0.002
Gly	7.692GlyAla: 7.692 ± 0.143	1.369GlyCys: 1.369 ± 0.062	4.045GlyAsp: 4.045 ± 0.097	5.598GlyGlu: 5.598 ± 0.117	3.164GlyPhe: 3.164 ± 0.084	6.505GlyGly: 6.505 ± 0.144	1.563GlyHis: 1.563 ± 0.061	4.907GlyIle: 4.907 ± 0.115	4.671GlyLys: 4.671 ± 0.108	6.997GlyLeu: 6.997 ± 0.149	2.6GlyMet: 2.6 ± 0.077	2.303GlyAsn: 2.303 ± 0.075	1.621GlyPro: 1.621 ± 0.053	2.208GlyGln: 2.208 ± 0.069	4.33GlyArg: 4.33 ± 0.122	4.405GlySer: 4.405 ± 0.098	5.057GlyThr: 5.057 ± 0.114	6.596GlyVal: 6.596 ± 0.138	0.884GlyTrp: 0.884 ± 0.043	3.06GlyTyr: 3.06 ± 0.085	0.004GlyXaa: 0.004 ± 0.003
His	1.747HisAla: 1.747 ± 0.063	0.423HisCys: 0.423 ± 0.032	1.074HisAsp: 1.074 ± 0.055	1.361HisGlu: 1.361 ± 0.054	0.752HisPhe: 0.752 ± 0.044	1.735HisGly: 1.735 ± 0.069	0.473HisHis: 0.473 ± 0.042	1.303HisIle: 1.303 ± 0.059	0.727HisLys: 0.727 ± 0.037	1.708HisLeu: 1.708 ± 0.056	0.454HisMet: 0.454 ± 0.03	0.589HisAsn: 0.589 ± 0.035	1.037HisPro: 1.037 ± 0.048	0.475HisGln: 0.475 ± 0.033	1.082HisArg: 1.082 ± 0.052	0.909HisSer: 0.909 ± 0.04	1.109HisThr: 1.109 ± 0.048	1.227HisVal: 1.227 ± 0.058	0.207HisTrp: 0.207 ± 0.018	0.7HisTyr: 0.7 ± 0.038	0.002HisXaa: 0.002 ± 0.002
Ile	5.824IleAla: 5.824 ± 0.111	0.923IleCys: 0.923 ± 0.043	3.347IleAsp: 3.347 ± 0.091	3.469IleGlu: 3.469 ± 0.075	1.962IlePhe: 1.962 ± 0.076	4.374IleGly: 4.374 ± 0.12	0.979IleHis: 0.979 ± 0.046	2.769IleIle: 2.769 ± 0.091	2.123IleLys: 2.123 ± 0.084	5.27IleLeu: 5.27 ± 0.13	1.315IleMet: 1.315 ± 0.05	1.65IleAsn: 1.65 ± 0.07	2.658IlePro: 2.658 ± 0.082	1.251IleGln: 1.251 ± 0.055	3.174IleArg: 3.174 ± 0.089	3.075IleSer: 3.075 ± 0.084	3.205IleThr: 3.205 ± 0.093	4.256IleVal: 4.256 ± 0.106	0.425IleTrp: 0.425 ± 0.033	1.664IleTyr: 1.664 ± 0.067	0.002IleXaa: 0.002 ± 0.002
Lys	4.459LysAla: 4.459 ± 0.115	0.636LysCys: 0.636 ± 0.039	2.267LysAsp: 2.267 ± 0.07	3.18LysGlu: 3.18 ± 0.099	1.239LysPhe: 1.239 ± 0.05	3.16LysGly: 3.16 ± 0.091	0.824LysHis: 0.824 ± 0.043	2.424LysIle: 2.424 ± 0.091	3.473LysLys: 3.473 ± 0.094	4.888LysLeu: 4.888 ± 0.116	1.528LysMet: 1.528 ± 0.058	1.949LysAsn: 1.949 ± 0.056	1.929LysPro: 1.929 ± 0.069	1.466LysGln: 1.466 ± 0.056	2.842LysArg: 2.842 ± 0.081	2.373LysSer: 2.373 ± 0.06	2.912LysThr: 2.912 ± 0.089	3.209LysVal: 3.209 ± 0.087	0.444LysTrp: 0.444 ± 0.031	1.625LysTyr: 1.625 ± 0.065	0.0LysXaa: 0.0 ± 0.0
Leu	10.646LeuAla: 10.646 ± 0.164	2.193LeuCys: 2.193 ± 0.073	5.64LeuAsp: 5.64 ± 0.112	5.836LeuGlu: 5.836 ± 0.113	3.878LeuPhe: 3.878 ± 0.105	7.546LeuGly: 7.546 ± 0.148	2.084LeuHis: 2.084 ± 0.067	4.9LeuIle: 4.9 ± 0.114	4.19LeuLys: 4.19 ± 0.106	11.306LeuLeu: 11.306 ± 0.231	2.474LeuMet: 2.474 ± 0.075	2.811LeuAsn: 2.811 ± 0.073	4.702LeuPro: 4.702 ± 0.085	2.548LeuGln: 2.548 ± 0.077	7.133LeuArg: 7.133 ± 0.162	6.563LeuSer: 6.563 ± 0.139	6.528LeuThr: 6.528 ± 0.125	6.468LeuVal: 6.468 ± 0.134	0.958LeuTrp: 0.958 ± 0.043	3.331LeuTyr: 3.331 ± 0.086	0.002LeuXaa: 0.002 ± 0.002
Met	2.978MetAla: 2.978 ± 0.079	0.353MetCys: 0.353 ± 0.026	1.735MetAsp: 1.735 ± 0.059	1.999MetGlu: 1.999 ± 0.066	0.847MetPhe: 0.847 ± 0.044	2.088MetGly: 2.088 ± 0.079	0.508MetHis: 0.508 ± 0.034	1.307MetIle: 1.307 ± 0.058	1.656MetLys: 1.656 ± 0.053	2.846MetLeu: 2.846 ± 0.087	0.789MetMet: 0.789 ± 0.046	1.064MetAsn: 1.064 ± 0.052	1.206MetPro: 1.206 ± 0.051	0.958MetGln: 0.958 ± 0.045	1.569MetArg: 1.569 ± 0.059	1.555MetSer: 1.555 ± 0.053	1.929MetThr: 1.929 ± 0.065	1.89MetVal: 1.89 ± 0.067	0.19MetTrp: 0.19 ± 0.022	0.63MetTyr: 0.63 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	3.335AsnAla: 3.335 ± 0.09	0.489AsnCys: 0.489 ± 0.03	1.759AsnAsp: 1.759 ± 0.058	1.991AsnGlu: 1.991 ± 0.064	1.125AsnPhe: 1.125 ± 0.045	3.091AsnGly: 3.091 ± 0.097	0.564AsnHis: 0.564 ± 0.032	1.863AsnIle: 1.863 ± 0.064	1.297AsnLys: 1.297 ± 0.05	2.683AsnLeu: 2.683 ± 0.075	0.898AsnMet: 0.898 ± 0.041	0.929AsnAsn: 0.929 ± 0.05	1.574AsnPro: 1.574 ± 0.051	0.733AsnGln: 0.733 ± 0.038	1.712AsnArg: 1.712 ± 0.071	1.351AsnSer: 1.351 ± 0.059	1.821AsnThr: 1.821 ± 0.066	2.34AsnVal: 2.34 ± 0.074	0.279AsnTrp: 0.279 ± 0.025	1.105AsnTyr: 1.105 ± 0.05	0.0AsnXaa: 0.0 ± 0.0
Pro	4.275ProAla: 4.275 ± 0.109	0.632ProCys: 0.632 ± 0.035	2.643ProAsp: 2.643 ± 0.072	3.55ProGlu: 3.55 ± 0.085	1.466ProPhe: 1.466 ± 0.059	2.625ProGly: 2.625 ± 0.08	0.776ProHis: 0.776 ± 0.046	2.057ProIle: 2.057 ± 0.07	1.733ProLys: 1.733 ± 0.067	3.663ProLeu: 3.663 ± 0.089	1.057ProMet: 1.057 ± 0.044	1.227ProAsn: 1.227 ± 0.048	1.233ProPro: 1.233 ± 0.049	1.336ProGln: 1.336 ± 0.046	1.923ProArg: 1.923 ± 0.062	1.91ProSer: 1.91 ± 0.063	2.104ProThr: 2.104 ± 0.075	3.081ProVal: 3.081 ± 0.082	0.376ProTrp: 0.376 ± 0.034	1.466ProTyr: 1.466 ± 0.059	0.0ProXaa: 0.0 ± 0.0
Gln	2.439GlnAla: 2.439 ± 0.08	0.386GlnCys: 0.386 ± 0.028	1.303GlnAsp: 1.303 ± 0.054	1.999GlnGlu: 1.999 ± 0.067	0.975GlnPhe: 0.975 ± 0.046	1.916GlnGly: 1.916 ± 0.063	0.525GlnHis: 0.525 ± 0.035	1.704GlnIle: 1.704 ± 0.06	1.714GlnLys: 1.714 ± 0.06	2.996GlnLeu: 2.996 ± 0.085	0.962GlnMet: 0.962 ± 0.043	1.08GlnAsn: 1.08 ± 0.047	1.051GlnPro: 1.051 ± 0.048	1.123GlnGln: 1.123 ± 0.053	1.935GlnArg: 1.935 ± 0.077	1.675GlnSer: 1.675 ± 0.056	1.714GlnThr: 1.714 ± 0.066	1.898GlnVal: 1.898 ± 0.069	0.293GlnTrp: 0.293 ± 0.027	1.09GlnTyr: 1.09 ± 0.056	0.002GlnXaa: 0.002 ± 0.002
Arg	6.098ArgAla: 6.098 ± 0.149	1.018ArgCys: 1.018 ± 0.052	3.147ArgAsp: 3.147 ± 0.085	5.21ArgGlu: 5.21 ± 0.126	2.476ArgPhe: 2.476 ± 0.067	4.438ArgGly: 4.438 ± 0.11	1.206ArgHis: 1.206 ± 0.057	3.3ArgIle: 3.3 ± 0.092	2.65ArgLys: 2.65 ± 0.08	5.751ArgLeu: 5.751 ± 0.126	1.753ArgMet: 1.753 ± 0.063	1.627ArgAsn: 1.627 ± 0.063	1.989ArgPro: 1.989 ± 0.063	1.887ArgGln: 1.887 ± 0.06	4.733ArgArg: 4.733 ± 0.116	3.213ArgSer: 3.213 ± 0.088	3.063ArgThr: 3.063 ± 0.077	4.2ArgVal: 4.2 ± 0.107	0.556ArgTrp: 0.556 ± 0.036	2.115ArgTyr: 2.115 ± 0.079	0.004ArgXaa: 0.004 ± 0.003
Ser	5.793SerAla: 5.793 ± 0.117	0.909SerCys: 0.909 ± 0.044	2.914SerAsp: 2.914 ± 0.088	3.207SerGlu: 3.207 ± 0.08	2.344SerPhe: 2.344 ± 0.071	5.092SerGly: 5.092 ± 0.093	0.935SerHis: 0.935 ± 0.042	2.875SerIle: 2.875 ± 0.093	2.046SerLys: 2.046 ± 0.071	5.202SerLeu: 5.202 ± 0.11	1.495SerMet: 1.495 ± 0.054	1.464SerAsn: 1.464 ± 0.054	1.997SerPro: 1.997 ± 0.071	1.307SerGln: 1.307 ± 0.049	3.073SerArg: 3.073 ± 0.08	2.705SerSer: 2.705 ± 0.093	2.563SerThr: 2.563 ± 0.081	4.32SerVal: 4.32 ± 0.084	0.489SerTrp: 0.489 ± 0.031	1.987SerTyr: 1.987 ± 0.071	0.0SerXaa: 0.0 ± 0.0
Thr	6.23ThrAla: 6.23 ± 0.119	0.655ThrCys: 0.655 ± 0.039	3.246ThrAsp: 3.246 ± 0.079	3.484ThrGlu: 3.484 ± 0.097	2.03ThrPhe: 2.03 ± 0.073	4.907ThrGly: 4.907 ± 0.118	1.018ThrHis: 1.018 ± 0.044	2.992ThrIle: 2.992 ± 0.088	2.042ThrLys: 2.042 ± 0.062	6.071ThrLeu: 6.071 ± 0.131	1.559ThrMet: 1.559 ± 0.049	1.576ThrAsn: 1.576 ± 0.062	2.819ThrPro: 2.819 ± 0.078	1.603ThrGln: 1.603 ± 0.058	2.674ThrArg: 2.674 ± 0.08	2.658ThrSer: 2.658 ± 0.081	2.864ThrThr: 2.864 ± 0.082	5.693ThrVal: 5.693 ± 0.134	0.491ThrTrp: 0.491 ± 0.034	1.807ThrTyr: 1.807 ± 0.057	0.002ThrXaa: 0.002 ± 0.002
Val	6.369ValAla: 6.369 ± 0.128	1.596ValCys: 1.596 ± 0.062	4.079ValAsp: 4.079 ± 0.098	4.814ValGlu: 4.814 ± 0.109	2.837ValPhe: 2.837 ± 0.08	5.598ValGly: 5.598 ± 0.144	1.293ValHis: 1.293 ± 0.054	4.143ValIle: 4.143 ± 0.086	3.327ValLys: 3.327 ± 0.084	7.874ValLeu: 7.874 ± 0.133	1.985ValMet: 1.985 ± 0.057	2.391ValAsn: 2.391 ± 0.073	3.137ValPro: 3.137 ± 0.083	1.857ValGln: 1.857 ± 0.069	4.551ValArg: 4.551 ± 0.105	4.591ValSer: 4.591 ± 0.112	4.977ValThr: 4.977 ± 0.133	5.406ValVal: 5.406 ± 0.148	0.694ValTrp: 0.694 ± 0.042	2.633ValTyr: 2.633 ± 0.075	0.0ValXaa: 0.0 ± 0.0
Trp	0.843TrpAla: 0.843 ± 0.05	0.231TrpCys: 0.231 ± 0.024	0.522TrpAsp: 0.522 ± 0.033	0.679TrpGlu: 0.679 ± 0.039	0.405TrpPhe: 0.405 ± 0.031	0.667TrpGly: 0.667 ± 0.043	0.207TrpHis: 0.207 ± 0.019	0.39TrpIle: 0.39 ± 0.033	0.368TrpLys: 0.368 ± 0.028	1.101TrpLeu: 1.101 ± 0.054	0.32TrpMet: 0.32 ± 0.027	0.372TrpAsn: 0.372 ± 0.029	0.235TrpPro: 0.235 ± 0.02	0.355TrpGln: 0.355 ± 0.03	0.667TrpArg: 0.667 ± 0.042	0.527TrpSer: 0.527 ± 0.032	0.427TrpThr: 0.427 ± 0.032	0.568TrpVal: 0.568 ± 0.032	0.116TrpTrp: 0.116 ± 0.017	0.372TrpTyr: 0.372 ± 0.032	0.002TrpXaa: 0.002 ± 0.002
Tyr	3.395TyrAla: 3.395 ± 0.078	0.597TyrCys: 0.597 ± 0.039	2.29TyrAsp: 2.29 ± 0.068	2.249TyrGlu: 2.249 ± 0.071	1.47TyrPhe: 1.47 ± 0.068	2.881TyrGly: 2.881 ± 0.081	0.684TyrHis: 0.684 ± 0.037	1.828TyrIle: 1.828 ± 0.065	1.218TyrLys: 1.218 ± 0.051	3.558TyrLeu: 3.558 ± 0.087	0.766TyrMet: 0.766 ± 0.037	1.134TyrAsn: 1.134 ± 0.053	1.392TyrPro: 1.392 ± 0.059	0.948TyrGln: 0.948 ± 0.044	2.267TyrArg: 2.267 ± 0.076	1.768TyrSer: 1.768 ± 0.062	2.013TyrThr: 2.013 ± 0.079	2.156TyrVal: 2.156 ± 0.067	0.361TyrTrp: 0.361 ± 0.029	1.39TyrTyr: 1.39 ± 0.063	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.01XaaAla: 0.01 ± 0.005	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.004XaaLeu: 0.004 ± 0.003	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.004XaaPro: 0.004 ± 0.003	0.0XaaGln: 0.0 ± 0.0	0.004XaaArg: 0.004 ± 0.003	0.002XaaSer: 0.002 ± 0.002	0.004XaaThr: 0.004 ± 0.003	0.002XaaVal: 0.002 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.047XaaXaa: 0.047 ± 0.015

Statistics based on 1635 proteins (484243 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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