Streptococcus phage CHPC1151
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L2JXU3|A0A1L2JXU3_9CAUD Tape-measure protein OS=Streptococcus phage CHPC1151 OX=1913083 GN=CHPC1151_0018 PE=4 SV=1
MM1 pKa = 7.13 VNWVDD6 pKa = 4.58 ANGNDD11 pKa = 3.45 IPDD14 pKa = 4.08 GKK16 pKa = 10.99 SEE18 pKa = 4.08 DD19 pKa = 4.06 FKK21 pKa = 11.35 PGMFFSFAGDD31 pKa = 3.45 EE32 pKa = 4.32 VNITDD37 pKa = 3.77 TGEE40 pKa = 3.65 GGYY43 pKa = 10.61 YY44 pKa = 9.76 GGYY47 pKa = 8.0 YY48 pKa = 9.41 YY49 pKa = 10.76 RR50 pKa = 11.84 RR51 pKa = 11.84 FEE53 pKa = 4.06 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.841
IPC2_protein 3.999
IPC_protein 3.897
Toseland 3.694
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.846
Nozaki 4.037
DTASelect 4.266
Thurlkill 3.77
EMBOSS 3.872
Sillero 4.024
Patrickios 0.477
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.925
Protein with the highest isoelectric point:
>tr|A0A1L2JXT4|A0A1L2JXT4_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC1151 OX=1913083 GN=CHPC1151_0014 PE=4 SV=1
MM1 pKa = 6.97 GTTVSVKK8 pKa = 10.13 VDD10 pKa = 2.99 LHH12 pKa = 7.19 GLEE15 pKa = 4.88 KK16 pKa = 10.49 KK17 pKa = 10.47 CSPEE21 pKa = 3.35 AVKK24 pKa = 10.43 RR25 pKa = 11.84 GKK27 pKa = 10.24 VAMIGQMITDD37 pKa = 4.47 MEE39 pKa = 4.37 PFIPRR44 pKa = 11.84 RR45 pKa = 11.84 DD46 pKa = 3.71 GTLSASGSPFSDD58 pKa = 4.77 GIRR61 pKa = 11.84 YY62 pKa = 7.2 PGPYY66 pKa = 10.0 ARR68 pKa = 11.84 AQFYY72 pKa = 10.7 GSSYY76 pKa = 10.12 NKK78 pKa = 9.31 NRR80 pKa = 11.84 SFVFRR85 pKa = 11.84 NYY87 pKa = 7.13 TTPGTGKK94 pKa = 9.94 RR95 pKa = 11.84 WDD97 pKa = 3.53 MKK99 pKa = 11.21 ASAKK103 pKa = 10.21 YY104 pKa = 8.51 SKK106 pKa = 10.29 QWGEE110 pKa = 3.73 VALRR114 pKa = 11.84 AMGVKK119 pKa = 10.0
Molecular weight: 13.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.201
IPC2_protein 9.56
IPC_protein 9.663
Toseland 10.218
ProMoST 9.926
Dawson 10.394
Bjellqvist 10.043
Wikipedia 10.555
Rodwell 10.877
Grimsley 10.452
Solomon 10.423
Lehninger 10.394
Nozaki 10.204
DTASelect 10.043
Thurlkill 10.248
EMBOSS 10.613
Sillero 10.306
Patrickios 10.57
IPC_peptide 10.423
IPC2_peptide 8.682
IPC2.peptide.svr19 8.59
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
10202
37
1528
221.8
24.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.518 ± 1.218
0.657 ± 0.132
5.852 ± 0.55
7.371 ± 0.851
4.078 ± 0.267
6.185 ± 0.648
1.166 ± 0.169
7.342 ± 0.426
8.224 ± 0.573
7.626 ± 0.333
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.892 ± 0.333
5.499 ± 0.375
2.754 ± 0.247
4.597 ± 0.357
4.087 ± 0.549
6.861 ± 0.607
6.283 ± 0.376
5.969 ± 0.271
1.019 ± 0.158
4.019 ± 0.529
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here