Catenaria anguillulae PL171
Average proteome isoelectric point is 7.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12599 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y2HUA0|A0A1Y2HUA0_9FUNG Uncharacterized protein OS=Catenaria anguillulae PL171 OX=765915 GN=BCR44DRAFT_41493 PE=4 SV=1
MM1 pKa = 7.15 SAAAAYY7 pKa = 9.95 YY8 pKa = 8.25 PLPCHH13 pKa = 6.04 STDD16 pKa = 3.26 ITAQTQAIDD25 pKa = 3.45 QSQVANAGDD34 pKa = 3.63 NSVIIQIQQAVQAQKK49 pKa = 11.47 ADD51 pKa = 3.74 VASTAAAGGRR61 pKa = 11.84 HH62 pKa = 5.42 MSDD65 pKa = 3.51 TYY67 pKa = 11.25 DD68 pKa = 3.36 QQDD71 pKa = 3.42 SVAQSQDD78 pKa = 3.08 LNQVQFANSGDD89 pKa = 3.6 NSVVIQNQNAVQSQFGNLDD108 pKa = 3.27 SSDD111 pKa = 3.23 GHH113 pKa = 6.38 GWATGNSIDD122 pKa = 4.92 QAQDD126 pKa = 3.12 LNQFQFANASDD137 pKa = 3.55 NSINQNAVQNQQANVGGGRR156 pKa = 11.84 HH157 pKa = 4.88 MHH159 pKa = 7.17 DD160 pKa = 4.12 ANTVAQAQDD169 pKa = 3.55 LNQLQFADD177 pKa = 4.31 AGDD180 pKa = 3.66 NSAIIQNQDD189 pKa = 2.69 APQSQFANIHH199 pKa = 6.3 DD200 pKa = 4.37 FGGHH204 pKa = 6.39 GYY206 pKa = 10.49 GHH208 pKa = 6.93 TGYY211 pKa = 8.96 PDD213 pKa = 3.69 SMITQAQDD221 pKa = 3.08 LTQLQFANAGDD232 pKa = 3.72 NAIIVQNQDD241 pKa = 2.57 AFQSQFANLFGGRR254 pKa = 11.84 HH255 pKa = 5.0 TSDD258 pKa = 3.8 ADD260 pKa = 4.38 VIAQAQNLDD269 pKa = 3.27 QFQFANAGDD278 pKa = 4.03 HH279 pKa = 6.85 SAVLQNQDD287 pKa = 3.13 ALQSQFALTNVDD299 pKa = 3.14 PHH301 pKa = 7.8 GYY303 pKa = 9.25 AASGGAGHH311 pKa = 7.57 AGGSDD316 pKa = 2.84 WLATFDD322 pKa = 4.42 FTHH325 pKa = 6.04 QVQDD329 pKa = 3.76 LNQVQFASAAGGDD342 pKa = 3.8 GGASAMHH349 pKa = 6.87 ASVMPDD355 pKa = 2.86 VTEE358 pKa = 4.21 PLVTMSLFF366 pKa = 3.55
Molecular weight: 38.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.821
IPC_protein 3.884
Toseland 3.63
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.923
Rodwell 3.706
Grimsley 3.528
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.393
Thurlkill 3.706
EMBOSS 3.923
Sillero 4.012
Patrickios 0.973
IPC_peptide 3.897
IPC2_peptide 3.986
IPC2.peptide.svr19 3.881
Protein with the highest isoelectric point:
>tr|A0A1Y2I6M2|A0A1Y2I6M2_9FUNG RRM domain-containing protein OS=Catenaria anguillulae PL171 OX=765915 GN=BCR44DRAFT_122977 PE=4 SV=1
MM1 pKa = 7.48 RR2 pKa = 11.84 RR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 9.74 RR7 pKa = 11.84 PRR9 pKa = 11.84 QQSRR13 pKa = 11.84 AMRR16 pKa = 11.84 RR17 pKa = 11.84 QPKK20 pKa = 10.01 LLRR23 pKa = 11.84 QPSRR27 pKa = 11.84 ATRR30 pKa = 11.84 NPPRR34 pKa = 11.84 LRR36 pKa = 11.84 RR37 pKa = 11.84 QPNLVMRR44 pKa = 11.84 NQRR47 pKa = 11.84 RR48 pKa = 11.84 LLRR51 pKa = 11.84 QPSRR55 pKa = 11.84 AMRR58 pKa = 11.84 NQPRR62 pKa = 11.84 RR63 pKa = 11.84 LHH65 pKa = 5.82 QPSQEE70 pKa = 4.25 TTSNQPRR77 pKa = 11.84 LPHH80 pKa = 5.41 QPSRR84 pKa = 11.84 VTMQHH89 pKa = 6.06 PSKK92 pKa = 10.51 RR93 pKa = 11.84 PPQPKK98 pKa = 8.78 PAKK101 pKa = 6.61 TTSPP105 pKa = 3.49
Molecular weight: 12.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.661
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.369
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.246
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12590
9
12599
4929253
49
5022
391.2
42.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.969 ± 0.029
1.443 ± 0.011
5.267 ± 0.018
4.509 ± 0.023
3.252 ± 0.015
6.533 ± 0.021
2.959 ± 0.013
3.908 ± 0.016
3.869 ± 0.022
9.305 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.376 ± 0.01
3.033 ± 0.012
6.816 ± 0.03
4.079 ± 0.017
6.211 ± 0.023
8.717 ± 0.032
6.163 ± 0.016
7.087 ± 0.019
1.352 ± 0.009
2.154 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here