Olive latent virus 2 (isolate Italy) (OLV-2)
Average proteome isoelectric point is 7.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q83945|Q83945_OLV2I Uncharacterized protein orf1 OS=Olive latent virus 2 (isolate Italy) OX=650489 GN=orf1 PE=4 SV=1
MM1 pKa = 7.61 AGVLYY6 pKa = 10.65 DD7 pKa = 3.77 EE8 pKa = 5.59 EE9 pKa = 4.8 YY10 pKa = 11.18 VPQEE14 pKa = 4.01 PDD16 pKa = 3.27 YY17 pKa = 11.09 LYY19 pKa = 10.92 CPYY22 pKa = 9.92 EE23 pKa = 4.12 WEE25 pKa = 5.04 GEE27 pKa = 4.17 DD28 pKa = 4.85 PTQCDD33 pKa = 3.34 ANVPSVSYY41 pKa = 10.55 LVEE44 pKa = 3.97 AVKK47 pKa = 10.74 KK48 pKa = 10.71 SFTEE52 pKa = 4.54 GNHH55 pKa = 6.77 DD56 pKa = 3.69 MLISCEE62 pKa = 4.23 SYY64 pKa = 10.44 HH65 pKa = 7.13 AEE67 pKa = 4.16 CCEE70 pKa = 3.92 GPLMNKK76 pKa = 9.87 GLTLSTYY83 pKa = 9.81 FMSYY87 pKa = 10.38 PGISLPAFGGGVIHH101 pKa = 5.6 VTSARR106 pKa = 11.84 VCMQAFYY113 pKa = 11.03 AVTPGRR119 pKa = 11.84 CSCSCGTQFIYY130 pKa = 10.22 RR131 pKa = 11.84 AKK133 pKa = 10.47 GGTGLTFDD141 pKa = 3.98 ATNTISSAFKK151 pKa = 10.99 FEE153 pKa = 4.47 DD154 pKa = 4.42 GKK156 pKa = 11.28 VKK158 pKa = 10.15 WLTEE162 pKa = 4.02 DD163 pKa = 3.92 LGLALLAGCFLKK175 pKa = 10.77 YY176 pKa = 10.77 VPIPHH181 pKa = 6.94 EE182 pKa = 3.88 IEE184 pKa = 3.62 IKK186 pKa = 10.1 IEE188 pKa = 4.08 RR189 pKa = 11.84 KK190 pKa = 9.67 VEE192 pKa = 3.85 PVSALDD198 pKa = 3.39 FWEE201 pKa = 4.52 PEE203 pKa = 3.91 GLISDD208 pKa = 3.61 VHH210 pKa = 5.88 FVHH213 pKa = 6.32 PQEE216 pKa = 4.4 RR217 pKa = 11.84 ALAPEE222 pKa = 4.29 PPMDD226 pKa = 3.53 VEE228 pKa = 4.35 MVRR231 pKa = 11.84 LVRR234 pKa = 11.84 TEE236 pKa = 3.9 LFEE239 pKa = 4.57 HH240 pKa = 6.78 FNPTLLLNARR250 pKa = 11.84 SDD252 pKa = 3.8 FSGEE256 pKa = 3.8 KK257 pKa = 10.06 LKK259 pKa = 10.86 EE260 pKa = 4.02 VEE262 pKa = 4.13 LPRR265 pKa = 11.84 AQSEE269 pKa = 4.23 PLVPRR274 pKa = 11.84 VCLPRR279 pKa = 11.84 GDD281 pKa = 4.97 DD282 pKa = 3.52 ALIDD286 pKa = 3.36 QFFIDD291 pKa = 4.5 KK292 pKa = 10.79 APEE295 pKa = 3.95 HH296 pKa = 6.53 FNRR299 pKa = 11.84 EE300 pKa = 3.88 PATDD304 pKa = 3.27 QWQVEE309 pKa = 4.42 SDD311 pKa = 3.91 DD312 pKa = 3.86 FTVDD316 pKa = 3.78 FPTQVRR322 pKa = 11.84 LRR324 pKa = 11.84 EE325 pKa = 3.85 RR326 pKa = 11.84 DD327 pKa = 3.42 EE328 pKa = 4.03 DD329 pKa = 3.55 WFRR332 pKa = 11.84 RR333 pKa = 11.84 KK334 pKa = 9.55 PDD336 pKa = 3.57 FAVSSIRR343 pKa = 11.84 TGLEE347 pKa = 3.46 MPRR350 pKa = 11.84 IATKK354 pKa = 10.78 KK355 pKa = 8.46 EE356 pKa = 3.36 MLMAFSKK363 pKa = 10.83 RR364 pKa = 11.84 NVGAPQMVEE373 pKa = 4.18 VPQEE377 pKa = 3.68 IHH379 pKa = 6.05 VKK381 pKa = 10.19 RR382 pKa = 11.84 LADD385 pKa = 3.5 RR386 pKa = 11.84 FFRR389 pKa = 11.84 TFLKK393 pKa = 10.69 EE394 pKa = 4.07 GGWDD398 pKa = 3.57 KK399 pKa = 11.8 GDD401 pKa = 3.03 GHH403 pKa = 6.72 YY404 pKa = 10.32 WDD406 pKa = 5.82 DD407 pKa = 3.55 FFHH410 pKa = 6.95 RR411 pKa = 11.84 KK412 pKa = 7.49 GRR414 pKa = 11.84 KK415 pKa = 8.71 VFSLEE420 pKa = 3.58 NCYY423 pKa = 10.9 DD424 pKa = 4.15 DD425 pKa = 5.14 PLSSYY430 pKa = 11.43 LVMLKK435 pKa = 10.42 SAGKK439 pKa = 10.22 FSLDD443 pKa = 2.97 LNFPVRR449 pKa = 11.84 SVPQTITYY457 pKa = 8.75 HH458 pKa = 5.57 EE459 pKa = 4.19 KK460 pKa = 9.41 WVTEE464 pKa = 4.02 VFSPMFLQVMARR476 pKa = 11.84 FFSRR480 pKa = 11.84 LQDD483 pKa = 3.43 WVVVPAGPMADD494 pKa = 4.91 FSRR497 pKa = 11.84 VWPSFEE503 pKa = 4.27 GKK505 pKa = 10.17 CLTEE509 pKa = 4.77 IDD511 pKa = 3.93 LSKK514 pKa = 10.64 FDD516 pKa = 3.92 KK517 pKa = 11.2 SQGKK521 pKa = 8.89 LLHH524 pKa = 6.67 DD525 pKa = 3.74 VQRR528 pKa = 11.84 EE529 pKa = 3.63 IFLRR533 pKa = 11.84 LGFPPIWCDD542 pKa = 2.18 WWFKK546 pKa = 9.99 FHH548 pKa = 7.01 EE549 pKa = 4.35 SSYY552 pKa = 11.54 LNDD555 pKa = 3.73 KK556 pKa = 10.64 NLGIAFSVDD565 pKa = 3.14 YY566 pKa = 10.6 QRR568 pKa = 11.84 RR569 pKa = 11.84 TGNTATYY576 pKa = 9.93 FGNTLVTMIMMAEE589 pKa = 4.2 VYY591 pKa = 10.08 EE592 pKa = 4.4 LDD594 pKa = 3.64 SLPFCGLFSGDD605 pKa = 4.24 DD606 pKa = 3.63 NLIVTDD612 pKa = 4.35 RR613 pKa = 11.84 PLEE616 pKa = 4.15 GRR618 pKa = 11.84 VSLFPNMFNMEE629 pKa = 4.64 AKK631 pKa = 10.31 VMRR634 pKa = 11.84 PGQNYY639 pKa = 7.12 MCSKK643 pKa = 10.01 YY644 pKa = 10.74 LCTVDD649 pKa = 4.98 GRR651 pKa = 11.84 VTPVPDD657 pKa = 3.86 PFKK660 pKa = 10.82 LLKK663 pKa = 10.22 KK664 pKa = 10.48 AGASCPLEE672 pKa = 4.36 HH673 pKa = 7.35 LDD675 pKa = 5.16 DD676 pKa = 4.88 FYY678 pKa = 11.78 EE679 pKa = 4.66 SFLDD683 pKa = 3.54 YY684 pKa = 10.71 TKK686 pKa = 11.2 YY687 pKa = 10.53 LVRR690 pKa = 11.84 DD691 pKa = 3.78 EE692 pKa = 4.33 VRR694 pKa = 11.84 TLIPRR699 pKa = 11.84 LMAHH703 pKa = 7.71 RR704 pKa = 11.84 YY705 pKa = 6.78 QVSMDD710 pKa = 3.62 EE711 pKa = 4.65 AGVALDD717 pKa = 5.63 QIMSWRR723 pKa = 11.84 KK724 pKa = 9.8 SKK726 pKa = 8.67 TQFFKK731 pKa = 10.63 HH732 pKa = 4.67 FQYY735 pKa = 10.72 RR736 pKa = 11.84 KK737 pKa = 8.84 PIKK740 pKa = 10.32 DD741 pKa = 3.13 SLEE744 pKa = 3.96 AVKK747 pKa = 10.75 SDD749 pKa = 3.4 FLSAFGKK756 pKa = 9.59 VDD758 pKa = 3.09 DD759 pKa = 5.69 RR760 pKa = 11.84 IRR762 pKa = 11.84 VWRR765 pKa = 11.84 GRR767 pKa = 11.84 RR768 pKa = 11.84 TNHH771 pKa = 6.02 KK772 pKa = 10.54 KK773 pKa = 10.23 EE774 pKa = 3.63 VDD776 pKa = 3.42 ALKK779 pKa = 10.94 VPLVSFRR786 pKa = 11.84 EE787 pKa = 4.17
Molecular weight: 90.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.465
IPC2_protein 5.474
IPC_protein 5.486
Toseland 5.703
ProMoST 5.703
Dawson 5.639
Bjellqvist 5.703
Wikipedia 5.588
Rodwell 5.588
Grimsley 5.766
Solomon 5.639
Lehninger 5.614
Nozaki 5.842
DTASelect 6.02
Thurlkill 5.919
EMBOSS 5.881
Sillero 5.931
Patrickios 4.126
IPC_peptide 5.652
IPC2_peptide 5.931
IPC2.peptide.svr19 5.93
Protein with the highest isoelectric point:
>tr|Q83946|Q83946_OLV2I Capsid protein OS=Olive latent virus 2 (isolate Italy) OX=650489 GN=orf2 PE=4 SV=1
MM1 pKa = 7.57 SSGDD5 pKa = 3.45 EE6 pKa = 4.17 TVPKK10 pKa = 10.38 LKK12 pKa = 10.17 QSKK15 pKa = 10.26 LSLDD19 pKa = 3.17 RR20 pKa = 11.84 HH21 pKa = 5.45 KK22 pKa = 11.16 ASWSYY27 pKa = 10.74 SVPWQRR33 pKa = 11.84 GRR35 pKa = 11.84 RR36 pKa = 11.84 FRR38 pKa = 11.84 VQTSHH43 pKa = 6.9 VGAEE47 pKa = 4.04 SQYY50 pKa = 9.33 PLLVATSSLLLSGAA64 pKa = 3.95
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.258
IPC2_protein 9.663
IPC_protein 10.116
Toseland 10.496
ProMoST 10.116
Dawson 10.613
Bjellqvist 10.277
Wikipedia 10.789
Rodwell 11.008
Grimsley 10.672
Solomon 10.687
Lehninger 10.657
Nozaki 10.452
DTASelect 10.277
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.526
Patrickios 10.833
IPC_peptide 10.687
IPC2_peptide 8.902
IPC2.peptide.svr19 8.747
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2453
64
908
408.8
46.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.194 ± 1.143
1.508 ± 0.598
5.503 ± 0.787
6.319 ± 0.622
5.544 ± 0.713
6.36 ± 1.016
2.731 ± 0.281
3.384 ± 0.319
5.3 ± 1.477
8.806 ± 0.535
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.609 ± 0.436
2.65 ± 0.183
5.096 ± 0.64
3.506 ± 0.378
6.767 ± 0.975
8.194 ± 1.102
4.77 ± 0.282
7.949 ± 0.876
1.631 ± 0.315
3.18 ± 0.376
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here