Paenisporosarcina sp. HGH0030

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Paenisporosarcina; unclassified Paenisporosarcina

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3445 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S2XHY7|S2XHY7_9BACL Uncharacterized protein OS=Paenisporosarcina sp. HGH0030 OX=1078085 GN=HMPREF1210_01722 PE=4 SV=1
MM1 pKa = 7.52DD2 pKa = 4.18QKK4 pKa = 10.15TMTDD8 pKa = 3.68YY9 pKa = 10.93IIQKK13 pKa = 8.03YY14 pKa = 8.77QEE16 pKa = 4.92DD17 pKa = 4.4EE18 pKa = 3.99HH19 pKa = 9.38VMIQLFVQWCQNYY32 pKa = 10.26EE33 pKa = 3.89IDD35 pKa = 5.31PLSLYY40 pKa = 10.12QKK42 pKa = 10.65AYY44 pKa = 6.26PTQGANEE51 pKa = 4.13TLKK54 pKa = 11.09SIIEE58 pKa = 4.12NNEE61 pKa = 4.3PIDD64 pKa = 4.47LDD66 pKa = 3.59IDD68 pKa = 3.74SGTLIEE74 pKa = 4.24ILQMFGNDD82 pKa = 3.28DD83 pKa = 3.85LAFMVSEE90 pKa = 4.46EE91 pKa = 4.06ANKK94 pKa = 10.48LL95 pKa = 3.56

Molecular weight: 11.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S2YGT6|S2YGT6_9BACL Uncharacterized protein OS=Paenisporosarcina sp. HGH0030 OX=1078085 GN=HMPREF1210_02205 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.18NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3445

0

3445

977063

29

2844

283.6

31.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.125 ± 0.043

0.619 ± 0.012

5.238 ± 0.036

7.336 ± 0.049

4.624 ± 0.033

6.796 ± 0.043

2.117 ± 0.02

7.567 ± 0.046

6.594 ± 0.039

9.577 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.91 ± 0.021

4.362 ± 0.036

3.655 ± 0.027

3.827 ± 0.029

3.941 ± 0.036

6.224 ± 0.032

5.778 ± 0.037

7.25 ± 0.039

1.054 ± 0.016

3.407 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski