Fictibacillus arsenicus
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3937 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B1Z0G5|A0A1B1Z0G5_9BACI Uncharacterized protein OS=Fictibacillus arsenicus OX=255247 GN=ABE41_002835 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 10.39 FCVTNHH8 pKa = 5.51 EE9 pKa = 4.46 LFVGEE14 pKa = 4.18 INIIGVGLSSVVLVGDD30 pKa = 4.39 ADD32 pKa = 4.5 CINLGSAFDD41 pKa = 4.18 TPPEE45 pKa = 4.07 SLVLGTSLVPLL56 pKa = 4.36
Molecular weight: 5.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.83
IPC2_protein 4.101
IPC_protein 3.846
Toseland 3.681
ProMoST 4.037
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.617
Solomon 3.795
Lehninger 3.745
Nozaki 4.012
DTASelect 4.126
Thurlkill 3.783
EMBOSS 3.821
Sillero 3.973
Patrickios 1.914
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.868
Protein with the highest isoelectric point:
>tr|A0A1B1YZ43|A0A1B1YZ43_9BACI DNA polymerase III subunit gamma/tau OS=Fictibacillus arsenicus OX=255247 GN=dnaX PE=3 SV=1
MM1 pKa = 7.6 KK2 pKa = 8.74 PTFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.22 KK14 pKa = 8.69 VHH16 pKa = 5.45 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 8.82 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3937
0
3937
1122737
26
1518
285.2
31.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.077 ± 0.04
0.704 ± 0.011
5.015 ± 0.03
7.584 ± 0.051
4.633 ± 0.038
7.0 ± 0.044
2.093 ± 0.018
7.526 ± 0.039
7.193 ± 0.04
9.765 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.819 ± 0.019
4.35 ± 0.025
3.714 ± 0.023
3.547 ± 0.025
3.879 ± 0.027
6.136 ± 0.031
5.382 ± 0.026
6.98 ± 0.029
1.089 ± 0.016
3.513 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here