Amino acid dipepetide frequency for Clostridium phage CDKM9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.169AlaAla: 2.169 ± 0.604
0.339AlaCys: 0.339 ± 0.179
2.711AlaAsp: 2.711 ± 0.519
4.473AlaGlu: 4.473 ± 0.525
2.507AlaPhe: 2.507 ± 0.52
2.846AlaGly: 2.846 ± 0.733
0.271AlaHis: 0.271 ± 0.13
4.744AlaIle: 4.744 ± 0.702
4.947AlaLys: 4.947 ± 0.533
5.693AlaLeu: 5.693 ± 0.775
1.762AlaMet: 1.762 ± 0.427
2.575AlaAsn: 2.575 ± 0.38
0.813AlaPro: 0.813 ± 0.232
1.491AlaGln: 1.491 ± 0.312
1.83AlaArg: 1.83 ± 0.381
4.405AlaSer: 4.405 ± 0.7
3.727AlaThr: 3.727 ± 0.606
2.914AlaVal: 2.914 ± 0.681
0.61AlaTrp: 0.61 ± 0.194
1.288AlaTyr: 1.288 ± 0.287
0.0AlaXaa: 0.0 ± 0.0
Cys
0.542CysAla: 0.542 ± 0.21
0.271CysCys: 0.271 ± 0.125
0.678CysAsp: 0.678 ± 0.211
0.813CysGlu: 0.813 ± 0.26
0.339CysPhe: 0.339 ± 0.192
0.678CysGly: 0.678 ± 0.225
0.136CysHis: 0.136 ± 0.102
1.423CysIle: 1.423 ± 0.281
1.355CysLys: 1.355 ± 0.374
0.542CysLeu: 0.542 ± 0.167
0.136CysMet: 0.136 ± 0.096
0.542CysAsn: 0.542 ± 0.187
0.271CysPro: 0.271 ± 0.13
0.339CysGln: 0.339 ± 0.162
0.407CysArg: 0.407 ± 0.171
0.407CysSer: 0.407 ± 0.137
0.474CysThr: 0.474 ± 0.173
0.474CysVal: 0.474 ± 0.186
0.136CysTrp: 0.136 ± 0.1
0.542CysTyr: 0.542 ± 0.171
0.0CysXaa: 0.0 ± 0.0
Asp
2.914AspAla: 2.914 ± 0.441
0.949AspCys: 0.949 ± 0.275
3.117AspAsp: 3.117 ± 0.608
6.031AspGlu: 6.031 ± 0.612
2.779AspPhe: 2.779 ± 0.49
3.388AspGly: 3.388 ± 0.481
0.068AspHis: 0.068 ± 0.061
6.235AspIle: 6.235 ± 0.687
6.574AspLys: 6.574 ± 0.785
3.456AspLeu: 3.456 ± 0.339
1.762AspMet: 1.762 ± 0.302
3.931AspAsn: 3.931 ± 0.557
0.678AspPro: 0.678 ± 0.248
0.474AspGln: 0.474 ± 0.192
2.101AspArg: 2.101 ± 0.41
3.388AspSer: 3.388 ± 0.394
2.846AspThr: 2.846 ± 0.455
2.982AspVal: 2.982 ± 0.462
0.474AspTrp: 0.474 ± 0.161
2.304AspTyr: 2.304 ± 0.425
0.0AspXaa: 0.0 ± 0.0
Glu
4.676GluAla: 4.676 ± 0.713
0.745GluCys: 0.745 ± 0.248
4.066GluAsp: 4.066 ± 0.526
7.726GluGlu: 7.726 ± 0.893
3.795GluPhe: 3.795 ± 0.535
4.473GluGly: 4.473 ± 0.547
0.745GluHis: 0.745 ± 0.226
7.522GluIle: 7.522 ± 0.964
8.81GluLys: 8.81 ± 0.932
9.962GluLeu: 9.962 ± 0.864
3.388GluMet: 3.388 ± 0.722
7.184GluAsn: 7.184 ± 0.956
1.423GluPro: 1.423 ± 0.349
2.575GluGln: 2.575 ± 0.374
3.253GluArg: 3.253 ± 0.48
3.66GluSer: 3.66 ± 0.493
4.405GluThr: 4.405 ± 0.627
5.557GluVal: 5.557 ± 0.815
0.745GluTrp: 0.745 ± 0.242
3.998GluTyr: 3.998 ± 0.67
0.0GluXaa: 0.0 ± 0.0
Phe
1.423PheAla: 1.423 ± 0.437
0.339PheCys: 0.339 ± 0.153
2.711PheAsp: 2.711 ± 0.354
3.66PheGlu: 3.66 ± 0.509
1.084PhePhe: 1.084 ± 0.256
2.575PheGly: 2.575 ± 0.384
0.474PheHis: 0.474 ± 0.188
4.202PheIle: 4.202 ± 0.539
3.998PheLys: 3.998 ± 0.482
3.253PheLeu: 3.253 ± 0.411
1.084PheMet: 1.084 ± 0.273
3.185PheAsn: 3.185 ± 0.529
1.017PhePro: 1.017 ± 0.325
0.813PheGln: 0.813 ± 0.236
1.559PheArg: 1.559 ± 0.363
2.846PheSer: 2.846 ± 0.416
2.304PheThr: 2.304 ± 0.379
2.643PheVal: 2.643 ± 0.6
0.136PheTrp: 0.136 ± 0.103
1.288PheTyr: 1.288 ± 0.32
0.0PheXaa: 0.0 ± 0.0
Gly
2.643GlyAla: 2.643 ± 0.61
0.745GlyCys: 0.745 ± 0.232
2.507GlyAsp: 2.507 ± 0.43
5.828GlyGlu: 5.828 ± 0.83
3.253GlyPhe: 3.253 ± 0.537
3.998GlyGly: 3.998 ± 0.931
0.949GlyHis: 0.949 ± 0.3
4.473GlyIle: 4.473 ± 0.834
5.354GlyLys: 5.354 ± 0.498
3.998GlyLeu: 3.998 ± 0.714
1.965GlyMet: 1.965 ± 0.323
3.592GlyAsn: 3.592 ± 0.444
0.542GlyPro: 0.542 ± 0.275
1.762GlyGln: 1.762 ± 0.429
2.101GlyArg: 2.101 ± 0.394
3.05GlySer: 3.05 ± 0.416
3.253GlyThr: 3.253 ± 0.673
4.202GlyVal: 4.202 ± 0.643
0.881GlyTrp: 0.881 ± 0.206
2.372GlyTyr: 2.372 ± 0.552
0.0GlyXaa: 0.0 ± 0.0
His
0.271HisAla: 0.271 ± 0.113
0.474HisCys: 0.474 ± 0.173
0.339HisAsp: 0.339 ± 0.145
0.745HisGlu: 0.745 ± 0.326
0.61HisPhe: 0.61 ± 0.221
0.339HisGly: 0.339 ± 0.151
0.068HisHis: 0.068 ± 0.085
0.542HisIle: 0.542 ± 0.197
1.084HisLys: 1.084 ± 0.339
1.017HisLeu: 1.017 ± 0.298
0.339HisMet: 0.339 ± 0.181
0.61HisAsn: 0.61 ± 0.305
0.474HisPro: 0.474 ± 0.211
0.271HisGln: 0.271 ± 0.151
0.474HisArg: 0.474 ± 0.183
0.542HisSer: 0.542 ± 0.166
0.678HisThr: 0.678 ± 0.217
0.339HisVal: 0.339 ± 0.137
0.068HisTrp: 0.068 ± 0.068
0.339HisTyr: 0.339 ± 0.148
0.0HisXaa: 0.0 ± 0.0
Ile
5.15IleAla: 5.15 ± 0.571
1.084IleCys: 1.084 ± 0.311
5.964IleAsp: 5.964 ± 0.846
7.455IleGlu: 7.455 ± 0.881
3.185IlePhe: 3.185 ± 0.472
4.541IleGly: 4.541 ± 0.523
0.61IleHis: 0.61 ± 0.16
5.828IleIle: 5.828 ± 0.754
10.369IleLys: 10.369 ± 0.972
7.455IleLeu: 7.455 ± 0.755
2.101IleMet: 2.101 ± 0.462
6.777IleAsn: 6.777 ± 0.616
2.101IlePro: 2.101 ± 0.33
2.44IleGln: 2.44 ± 0.448
3.456IleArg: 3.456 ± 0.569
6.506IleSer: 6.506 ± 1.201
4.269IleThr: 4.269 ± 0.535
5.015IleVal: 5.015 ± 0.697
0.745IleTrp: 0.745 ± 0.262
3.524IleTyr: 3.524 ± 0.591
0.0IleXaa: 0.0 ± 0.0
Lys
6.303LysAla: 6.303 ± 0.743
0.745LysCys: 0.745 ± 0.258
6.845LysAsp: 6.845 ± 0.712
11.995LysGlu: 11.995 ± 0.914
3.05LysPhe: 3.05 ± 0.4
6.37LysGly: 6.37 ± 0.586
1.152LysHis: 1.152 ± 0.337
9.555LysIle: 9.555 ± 0.807
10.979LysLys: 10.979 ± 1.342
8.674LysLeu: 8.674 ± 0.779
2.101LysMet: 2.101 ± 0.444
7.793LysAsn: 7.793 ± 0.848
2.033LysPro: 2.033 ± 0.481
3.795LysGln: 3.795 ± 0.539
4.134LysArg: 4.134 ± 0.586
6.777LysSer: 6.777 ± 0.76
4.744LysThr: 4.744 ± 0.43
6.98LysVal: 6.98 ± 0.605
1.084LysTrp: 1.084 ± 0.258
3.66LysTyr: 3.66 ± 0.596
0.0LysXaa: 0.0 ± 0.0
Leu
4.134LeuAla: 4.134 ± 0.533
0.881LeuCys: 0.881 ± 0.236
5.354LeuAsp: 5.354 ± 0.65
7.726LeuGlu: 7.726 ± 0.926
3.253LeuPhe: 3.253 ± 0.488
5.896LeuGly: 5.896 ± 0.745
1.017LeuHis: 1.017 ± 0.318
6.777LeuIle: 6.777 ± 0.721
11.046LeuLys: 11.046 ± 1.011
6.574LeuLeu: 6.574 ± 0.867
1.152LeuMet: 1.152 ± 0.224
5.557LeuAsn: 5.557 ± 0.632
1.762LeuPro: 1.762 ± 0.373
2.575LeuGln: 2.575 ± 0.407
3.66LeuArg: 3.66 ± 0.555
5.76LeuSer: 5.76 ± 0.685
4.879LeuThr: 4.879 ± 0.707
4.541LeuVal: 4.541 ± 0.547
0.61LeuTrp: 0.61 ± 0.175
2.914LeuTyr: 2.914 ± 0.461
0.0LeuXaa: 0.0 ± 0.0
Met
1.626MetAla: 1.626 ± 0.421
0.136MetCys: 0.136 ± 0.122
1.355MetAsp: 1.355 ± 0.363
1.559MetGlu: 1.559 ± 0.285
0.542MetPhe: 0.542 ± 0.165
1.017MetGly: 1.017 ± 0.286
0.136MetHis: 0.136 ± 0.09
1.626MetIle: 1.626 ± 0.361
3.05MetLys: 3.05 ± 0.404
2.372MetLeu: 2.372 ± 0.479
0.339MetMet: 0.339 ± 0.151
1.762MetAsn: 1.762 ± 0.349
0.61MetPro: 0.61 ± 0.142
0.542MetGln: 0.542 ± 0.172
0.881MetArg: 0.881 ± 0.23
1.491MetSer: 1.491 ± 0.354
1.355MetThr: 1.355 ± 0.289
1.288MetVal: 1.288 ± 0.241
0.339MetTrp: 0.339 ± 0.13
1.22MetTyr: 1.22 ± 0.249
0.0MetXaa: 0.0 ± 0.0
Asn
4.066AsnAla: 4.066 ± 0.666
0.542AsnCys: 0.542 ± 0.159
3.05AsnAsp: 3.05 ± 0.508
5.083AsnGlu: 5.083 ± 0.793
2.914AsnPhe: 2.914 ± 0.396
4.541AsnGly: 4.541 ± 0.641
0.474AsnHis: 0.474 ± 0.197
6.98AsnIle: 6.98 ± 0.755
7.726AsnLys: 7.726 ± 0.781
5.489AsnLeu: 5.489 ± 0.548
1.694AsnMet: 1.694 ± 0.323
4.405AsnAsn: 4.405 ± 0.569
2.033AsnPro: 2.033 ± 0.372
1.22AsnGln: 1.22 ± 0.387
3.185AsnArg: 3.185 ± 0.38
4.134AsnSer: 4.134 ± 0.576
3.117AsnThr: 3.117 ± 0.528
4.879AsnVal: 4.879 ± 0.488
0.745AsnTrp: 0.745 ± 0.244
1.559AsnTyr: 1.559 ± 0.335
0.0AsnXaa: 0.0 ± 0.0
Pro
0.813ProAla: 0.813 ± 0.246
0.339ProCys: 0.339 ± 0.146
0.949ProAsp: 0.949 ± 0.248
1.22ProGlu: 1.22 ± 0.314
0.881ProPhe: 0.881 ± 0.2
0.745ProGly: 0.745 ± 0.181
0.339ProHis: 0.339 ± 0.175
2.779ProIle: 2.779 ± 0.418
2.44ProLys: 2.44 ± 0.42
1.559ProLeu: 1.559 ± 0.419
0.203ProMet: 0.203 ± 0.116
1.288ProAsn: 1.288 ± 0.352
0.271ProPro: 0.271 ± 0.131
0.881ProGln: 0.881 ± 0.212
0.949ProArg: 0.949 ± 0.291
1.084ProSer: 1.084 ± 0.285
1.965ProThr: 1.965 ± 0.308
1.22ProVal: 1.22 ± 0.255
0.136ProTrp: 0.136 ± 0.091
0.813ProTyr: 0.813 ± 0.318
0.0ProXaa: 0.0 ± 0.0
Gln
1.762GlnAla: 1.762 ± 0.428
0.136GlnCys: 0.136 ± 0.11
1.423GlnAsp: 1.423 ± 0.393
2.711GlnGlu: 2.711 ± 0.44
0.949GlnPhe: 0.949 ± 0.209
1.694GlnGly: 1.694 ± 0.323
0.136GlnHis: 0.136 ± 0.102
2.779GlnIle: 2.779 ± 0.431
2.507GlnLys: 2.507 ± 0.6
2.575GlnLeu: 2.575 ± 0.396
0.542GlnMet: 0.542 ± 0.17
2.304GlnAsn: 2.304 ± 0.356
0.678GlnPro: 0.678 ± 0.276
1.152GlnGln: 1.152 ± 0.335
0.61GlnArg: 0.61 ± 0.245
1.626GlnSer: 1.626 ± 0.459
1.965GlnThr: 1.965 ± 0.303
1.22GlnVal: 1.22 ± 0.287
0.271GlnTrp: 0.271 ± 0.132
1.22GlnTyr: 1.22 ± 0.313
0.0GlnXaa: 0.0 ± 0.0
Arg
1.762ArgAla: 1.762 ± 0.398
0.542ArgCys: 0.542 ± 0.207
1.898ArgAsp: 1.898 ± 0.378
4.405ArgGlu: 4.405 ± 0.582
1.288ArgPhe: 1.288 ± 0.282
1.83ArgGly: 1.83 ± 0.397
0.407ArgHis: 0.407 ± 0.169
3.321ArgIle: 3.321 ± 0.469
4.066ArgLys: 4.066 ± 0.615
3.66ArgLeu: 3.66 ± 0.593
1.626ArgMet: 1.626 ± 0.328
1.762ArgAsn: 1.762 ± 0.366
0.881ArgPro: 0.881 ± 0.288
0.881ArgGln: 0.881 ± 0.186
1.152ArgArg: 1.152 ± 0.399
1.626ArgSer: 1.626 ± 0.374
1.83ArgThr: 1.83 ± 0.408
2.643ArgVal: 2.643 ± 0.363
0.474ArgTrp: 0.474 ± 0.159
1.355ArgTyr: 1.355 ± 0.254
0.0ArgXaa: 0.0 ± 0.0
Ser
3.524SerAla: 3.524 ± 0.548
0.339SerCys: 0.339 ± 0.142
3.456SerAsp: 3.456 ± 0.513
4.541SerGlu: 4.541 ± 0.493
3.321SerPhe: 3.321 ± 0.642
3.795SerGly: 3.795 ± 0.658
0.745SerHis: 0.745 ± 0.28
5.422SerIle: 5.422 ± 0.837
6.641SerLys: 6.641 ± 0.715
5.15SerLeu: 5.15 ± 0.52
1.152SerMet: 1.152 ± 0.284
4.812SerAsn: 4.812 ± 0.846
1.355SerPro: 1.355 ± 0.256
1.898SerGln: 1.898 ± 0.362
1.898SerArg: 1.898 ± 0.317
5.354SerSer: 5.354 ± 0.771
3.05SerThr: 3.05 ± 0.455
3.456SerVal: 3.456 ± 0.513
0.271SerTrp: 0.271 ± 0.132
2.575SerTyr: 2.575 ± 0.516
0.0SerXaa: 0.0 ± 0.0
Thr
3.456ThrAla: 3.456 ± 0.643
0.474ThrCys: 0.474 ± 0.24
2.711ThrAsp: 2.711 ± 0.466
3.388ThrGlu: 3.388 ± 0.527
2.304ThrPhe: 2.304 ± 0.389
2.711ThrGly: 2.711 ± 0.555
0.949ThrHis: 0.949 ± 0.239
5.083ThrIle: 5.083 ± 0.669
6.438ThrLys: 6.438 ± 0.656
5.083ThrLeu: 5.083 ± 0.491
0.678ThrMet: 0.678 ± 0.257
2.643ThrAsn: 2.643 ± 0.403
1.83ThrPro: 1.83 ± 0.394
2.033ThrGln: 2.033 ± 0.405
1.762ThrArg: 1.762 ± 0.322
4.202ThrSer: 4.202 ± 0.488
2.982ThrThr: 2.982 ± 0.508
3.321ThrVal: 3.321 ± 0.521
0.407ThrTrp: 0.407 ± 0.15
1.762ThrTyr: 1.762 ± 0.37
0.0ThrXaa: 0.0 ± 0.0
Val
2.914ValAla: 2.914 ± 0.497
0.61ValCys: 0.61 ± 0.216
4.473ValAsp: 4.473 ± 0.594
4.879ValGlu: 4.879 ± 0.557
2.507ValPhe: 2.507 ± 0.547
4.066ValGly: 4.066 ± 0.834
0.407ValHis: 0.407 ± 0.152
5.218ValIle: 5.218 ± 0.733
5.76ValLys: 5.76 ± 0.663
5.15ValLeu: 5.15 ± 0.546
0.678ValMet: 0.678 ± 0.194
4.202ValAsn: 4.202 ± 0.484
0.813ValPro: 0.813 ± 0.23
1.491ValGln: 1.491 ± 0.314
2.304ValArg: 2.304 ± 0.336
3.66ValSer: 3.66 ± 0.551
3.727ValThr: 3.727 ± 0.47
3.998ValVal: 3.998 ± 0.778
0.678ValTrp: 0.678 ± 0.265
2.236ValTyr: 2.236 ± 0.465
0.0ValXaa: 0.0 ± 0.0
Trp
0.542TrpAla: 0.542 ± 0.189
0.271TrpCys: 0.271 ± 0.131
0.745TrpAsp: 0.745 ± 0.204
0.949TrpGlu: 0.949 ± 0.2
0.339TrpPhe: 0.339 ± 0.136
0.407TrpGly: 0.407 ± 0.154
0.068TrpHis: 0.068 ± 0.069
0.813TrpIle: 0.813 ± 0.277
0.813TrpLys: 0.813 ± 0.174
0.813TrpLeu: 0.813 ± 0.271
0.068TrpMet: 0.068 ± 0.083
0.678TrpAsn: 0.678 ± 0.214
0.068TrpPro: 0.068 ± 0.056
0.339TrpGln: 0.339 ± 0.141
0.339TrpArg: 0.339 ± 0.147
0.339TrpSer: 0.339 ± 0.121
0.407TrpThr: 0.407 ± 0.168
0.678TrpVal: 0.678 ± 0.201
0.203TrpTrp: 0.203 ± 0.133
0.542TrpTyr: 0.542 ± 0.243
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.423TyrAla: 1.423 ± 0.355
0.542TyrCys: 0.542 ± 0.208
2.101TyrAsp: 2.101 ± 0.497
3.524TyrGlu: 3.524 ± 0.568
1.694TyrPhe: 1.694 ± 0.363
1.559TyrGly: 1.559 ± 0.396
0.474TyrHis: 0.474 ± 0.205
3.253TyrIle: 3.253 ± 0.478
4.879TyrLys: 4.879 ± 0.798
3.592TyrLeu: 3.592 ± 0.505
0.407TyrMet: 0.407 ± 0.163
2.236TyrAsn: 2.236 ± 0.394
1.22TyrPro: 1.22 ± 0.312
1.288TyrGln: 1.288 ± 0.278
1.355TyrArg: 1.355 ± 0.304
1.898TyrSer: 1.898 ± 0.405
2.236TyrThr: 2.236 ± 0.479
1.423TyrVal: 1.423 ± 0.309
0.407TyrTrp: 0.407 ± 0.184
1.83TyrTyr: 1.83 ± 0.406
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (14757 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski