Elm carlavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus; unclassified Carlavirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R7Z6N6|A0A2R7Z6N6_9VIRU 7 kDa protein OS=Elm carlavirus OX=2015551 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.54NSVLDD6 pKa = 3.77VLCEE10 pKa = 4.13FGFSRR15 pKa = 11.84ISISSEE21 pKa = 3.58RR22 pKa = 11.84PLVVHH27 pKa = 6.5CVPGAGKK34 pKa = 8.41STAIRR39 pKa = 11.84SILRR43 pKa = 11.84SCEE46 pKa = 3.47NSVAYY51 pKa = 9.19TFGVCDD57 pKa = 3.4PAQIEE62 pKa = 4.68GCRR65 pKa = 11.84IRR67 pKa = 11.84SASEE71 pKa = 3.17WHH73 pKa = 6.63EE74 pKa = 4.43PNSGTIVIVDD84 pKa = 4.73EE85 pKa = 4.2YY86 pKa = 11.22TEE88 pKa = 4.77GDD90 pKa = 3.98FKK92 pKa = 11.22SLKK95 pKa = 7.52PTVVFGDD102 pKa = 3.99PCQAPVSAITLKK114 pKa = 10.61PHH116 pKa = 7.19FICKK120 pKa = 6.83STKK123 pKa = 9.57RR124 pKa = 11.84FGKK127 pKa = 7.35EE128 pKa = 3.33TCNLLRR134 pKa = 11.84SLGYY138 pKa = 10.11EE139 pKa = 3.81INSEE143 pKa = 4.01KK144 pKa = 10.9GDD146 pKa = 3.96TLVHH150 pKa = 5.81EE151 pKa = 5.33PIFEE155 pKa = 4.42GEE157 pKa = 4.09LEE159 pKa = 4.18GVIVAFEE166 pKa = 4.2SDD168 pKa = 4.0VKK170 pKa = 11.03HH171 pKa = 6.34LLAEE175 pKa = 4.89HH176 pKa = 6.07YY177 pKa = 10.85CEE179 pKa = 5.41FLDD182 pKa = 3.6ICEE185 pKa = 4.15IRR187 pKa = 11.84GKK189 pKa = 8.06TFEE192 pKa = 4.16VVTFVTSTEE201 pKa = 4.08GKK203 pKa = 8.91PVKK206 pKa = 10.36NLEE209 pKa = 4.37KK210 pKa = 10.58YY211 pKa = 7.39YY212 pKa = 10.71QCLTRR217 pKa = 11.84HH218 pKa = 5.03SSKK221 pKa = 10.99LKK223 pKa = 10.28ILSPDD228 pKa = 2.96ASFASSS234 pKa = 2.9

Molecular weight: 25.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R7Z6N1|A0A2R7Z6N1_9VIRU Helicase OS=Elm carlavirus OX=2015551 GN=replicase PE=3 SV=1
MM1 pKa = 7.65PLSPPADD8 pKa = 3.42KK9 pKa = 10.74TSVYY13 pKa = 9.54FASAIGLGIALSLFTLTRR31 pKa = 11.84STLPHH36 pKa = 6.64VGDD39 pKa = 4.96NIHH42 pKa = 6.4SLPHH46 pKa = 5.54GGRR49 pKa = 11.84YY50 pKa = 8.92RR51 pKa = 11.84DD52 pKa = 3.63GTKK55 pKa = 10.42SVDD58 pKa = 3.28YY59 pKa = 10.53CGPAKK64 pKa = 10.33RR65 pKa = 11.84YY66 pKa = 8.42PSSNLLTSGSSSTAVLFIIVLVGLIWLSDD95 pKa = 3.36KK96 pKa = 10.7FGKK99 pKa = 10.39RR100 pKa = 11.84GVCNNCNRR108 pKa = 11.84SHH110 pKa = 5.62QCC112 pKa = 3.38

Molecular weight: 11.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2638

71

1915

527.6

59.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.255 ± 0.294

2.881 ± 0.37

5.648 ± 0.449

6.823 ± 0.982

5.951 ± 0.892

6.899 ± 0.578

2.578 ± 0.232

5.345 ± 0.501

7.771 ± 0.649

9.098 ± 0.851

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.414

4.094 ± 0.425

3.791 ± 0.578

2.35 ± 0.484

4.966 ± 0.296

7.24 ± 1.426

5.383 ± 0.635

6.52 ± 0.464

1.061 ± 0.197

2.881 ± 0.591
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski