Capybara microvirus Cap3_SP_332
Average proteome isoelectric point is 5.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5N2|A0A4P8W5N2_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_332 OX=2585430 PE=4 SV=1
MM1 pKa = 6.8 ITKK4 pKa = 10.41 NSIKK8 pKa = 10.61 LSILNFLKK16 pKa = 10.8 DD17 pKa = 3.59 NPNFNRR23 pKa = 11.84 INTLIDD29 pKa = 3.51 YY30 pKa = 10.21 LKK32 pKa = 10.32 FDD34 pKa = 4.8 PDD36 pKa = 3.87 SFVHH40 pKa = 6.32 SFEE43 pKa = 5.27 DD44 pKa = 3.06 NGVFVDD50 pKa = 5.67 LKK52 pKa = 9.75 FTPTSYY58 pKa = 11.46 NDD60 pKa = 3.53 YY61 pKa = 10.39 LISALSFADD70 pKa = 3.11 QVIFHH75 pKa = 6.28 SFKK78 pKa = 11.14 DD79 pKa = 3.59 VIEE82 pKa = 4.17 VVFFFEE88 pKa = 4.22 PTNDD92 pKa = 3.08 FKK94 pKa = 11.5 FIVYY98 pKa = 9.84 GIFDD102 pKa = 5.49 DD103 pKa = 6.18 IIPTGNTWADD113 pKa = 3.08 MTFYY117 pKa = 10.69 FEE119 pKa = 4.98 SCIEE123 pKa = 3.79 PDD125 pKa = 3.95 YY126 pKa = 10.27 YY127 pKa = 10.28 ISMYY131 pKa = 10.67 EE132 pKa = 3.91 EE133 pKa = 4.42 YY134 pKa = 10.39 EE135 pKa = 3.98
Molecular weight: 15.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 4.012
IPC_protein 3.986
Toseland 3.77
ProMoST 4.101
Dawson 3.973
Bjellqvist 4.177
Wikipedia 3.91
Rodwell 3.808
Grimsley 3.681
Solomon 3.961
Lehninger 3.923
Nozaki 4.088
DTASelect 4.329
Thurlkill 3.821
EMBOSS 3.923
Sillero 4.101
Patrickios 1.036
IPC_peptide 3.961
IPC2_peptide 4.075
IPC2.peptide.svr19 4.008
Protein with the highest isoelectric point:
>tr|A0A4V1FVS2|A0A4V1FVS2_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_332 OX=2585430 PE=4 SV=1
MM1 pKa = 7.16 CTSPLLIKK9 pKa = 10.74 NPNCLLGLHH18 pKa = 7.09 DD19 pKa = 5.66 GLAKK23 pKa = 10.05 SLKK26 pKa = 10.57 GITNTIKK33 pKa = 10.51 HH34 pKa = 5.76 PQSGFKK40 pKa = 10.3 DD41 pKa = 3.67 YY42 pKa = 10.9 LSQYY46 pKa = 10.49 IYY48 pKa = 10.56 VPCGKK53 pKa = 10.14 CPEE56 pKa = 4.52 CLNSKK61 pKa = 10.42 QNDD64 pKa = 3.38 IIQRR68 pKa = 11.84 VSFEE72 pKa = 3.91 QLTSYY77 pKa = 11.1 EE78 pKa = 4.4 FFCTLTVKK86 pKa = 10.61 PGFLDD91 pKa = 3.67 SAVLPDD97 pKa = 3.55 SSHH100 pKa = 6.6 YY101 pKa = 11.05 YY102 pKa = 9.32 YY103 pKa = 11.18 LDD105 pKa = 3.2 WSKK108 pKa = 11.73 LDD110 pKa = 3.99 LFFKK114 pKa = 10.78 RR115 pKa = 11.84 LRR117 pKa = 11.84 NKK119 pKa = 10.56 NIFDD123 pKa = 4.09 GPFKK127 pKa = 10.82 YY128 pKa = 10.39 LAVQEE133 pKa = 5.04 FGHH136 pKa = 5.64 EE137 pKa = 3.8 THH139 pKa = 6.98 RR140 pKa = 11.84 PHH142 pKa = 6.41 FHH144 pKa = 5.99 VLFFIPRR151 pKa = 11.84 LGKK154 pKa = 10.36 DD155 pKa = 2.89 ILWPYY160 pKa = 10.39 RR161 pKa = 11.84 CVDD164 pKa = 3.56 EE165 pKa = 5.73 NFDD168 pKa = 3.91 ILSNHH173 pKa = 5.76 QIKK176 pKa = 10.62 SRR178 pKa = 11.84 ILDD181 pKa = 3.0 EE182 pKa = 4.16 WYY184 pKa = 9.78 INKK187 pKa = 9.68 GSKK190 pKa = 10.06 RR191 pKa = 11.84 NPQPEE196 pKa = 4.28 PLTEE200 pKa = 4.16 FFTYY204 pKa = 10.1 CGRR207 pKa = 11.84 RR208 pKa = 11.84 NFDD211 pKa = 3.34 FSFVDD216 pKa = 3.72 PKK218 pKa = 10.66 TSQDD222 pKa = 3.57 LAVDD226 pKa = 4.87 FYY228 pKa = 11.62 ASKK231 pKa = 11.01 YY232 pKa = 9.8 ALKK235 pKa = 10.47 PDD237 pKa = 3.14 DD238 pKa = 4.13 WLNKK242 pKa = 8.97 KK243 pKa = 10.35 LSYY246 pKa = 10.35 LLYY249 pKa = 9.61 MSYY252 pKa = 11.0 NFYY255 pKa = 9.59 STKK258 pKa = 10.75 DD259 pKa = 3.33 NPYY262 pKa = 8.16 YY263 pKa = 10.48 LAYY266 pKa = 9.75 TRR268 pKa = 11.84 MRR270 pKa = 11.84 PRR272 pKa = 11.84 IYY274 pKa = 9.66 MSHH277 pKa = 6.3 QLGCSDD283 pKa = 3.09 STRR286 pKa = 11.84 RR287 pKa = 11.84 YY288 pKa = 9.68 IEE290 pKa = 5.1 RR291 pKa = 11.84 EE292 pKa = 3.82 TLDD295 pKa = 4.57 NICLNYY301 pKa = 9.39 PYY303 pKa = 10.28 PVIHH307 pKa = 6.62 SPADD311 pKa = 3.15 GRR313 pKa = 11.84 TYY315 pKa = 10.6 PLSAYY320 pKa = 9.22 FRR322 pKa = 11.84 KK323 pKa = 10.15 KK324 pKa = 10.0 YY325 pKa = 10.69 LPARR329 pKa = 11.84 WYY331 pKa = 10.51 DD332 pKa = 4.19 DD333 pKa = 3.06 MLLYY337 pKa = 9.84 YY338 pKa = 10.15 QRR340 pKa = 11.84 NNMPLATFTHH350 pKa = 6.54 NEE352 pKa = 3.88 DD353 pKa = 2.96 KK354 pKa = 10.98 HH355 pKa = 5.84 RR356 pKa = 11.84 QNAINFDD363 pKa = 4.07 KK364 pKa = 10.67 KK365 pKa = 10.34 CKK367 pKa = 10.1 KK368 pKa = 9.93 ILEE371 pKa = 4.11 HH372 pKa = 7.19 HH373 pKa = 6.48 ILL375 pKa = 3.76
Molecular weight: 44.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.183
IPC2_protein 8.2
IPC_protein 8.053
Toseland 8.009
ProMoST 8.551
Dawson 8.785
Bjellqvist 8.946
Wikipedia 8.829
Rodwell 8.843
Grimsley 8.083
Solomon 8.858
Lehninger 8.829
Nozaki 9.019
DTASelect 8.756
Thurlkill 8.799
EMBOSS 8.931
Sillero 9.048
Patrickios 3.719
IPC_peptide 8.843
IPC2_peptide 7.849
IPC2.peptide.svr19 7.97
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1834
71
640
262.0
30.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.344 ± 1.353
1.527 ± 0.519
7.361 ± 0.39
3.98 ± 0.523
6.543 ± 0.873
4.526 ± 0.735
1.854 ± 0.524
6.052 ± 0.917
5.616 ± 0.564
8.342 ± 0.596
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.617 ± 0.35
7.306 ± 0.418
4.253 ± 0.665
3.762 ± 1.135
4.144 ± 0.473
8.724 ± 0.604
5.125 ± 0.571
5.507 ± 1.194
1.2 ± 0.161
6.216 ± 0.813
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here