Brucella phage EF4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Perisivirus; unclassified Perisivirus

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6C0X1C1|A0A6C0X1C1_9CAUD Uncharacterized protein OS=Brucella phage EF4 OX=2706778 PE=4 SV=1
MM1 pKa = 7.53ASEE4 pKa = 5.34LEE6 pKa = 4.12PCPFCSKK13 pKa = 9.42TMMLRR18 pKa = 11.84SALWPSEE25 pKa = 3.46GDD27 pKa = 3.02ADD29 pKa = 5.13AIIHH33 pKa = 7.05ADD35 pKa = 3.55PTDD38 pKa = 3.84CPMLGFSDD46 pKa = 5.0GSADD50 pKa = 3.73GSIIEE55 pKa = 4.64KK56 pKa = 9.65WNCSLSNEE64 pKa = 4.61DD65 pKa = 5.2DD66 pKa = 4.07FPSDD70 pKa = 4.06GSCVRR75 pKa = 11.84CGSVPRR81 pKa = 11.84NANGLCNTCLDD92 pKa = 3.84EE93 pKa = 4.7DD94 pKa = 4.11AEE96 pKa = 4.64RR97 pKa = 11.84IEE99 pKa = 4.33NTRR102 pKa = 11.84PTPRR106 pKa = 11.84YY107 pKa = 10.04SSIAGCC113 pKa = 4.07

Molecular weight: 12.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6C0X1D1|A0A6C0X1D1_9CAUD Uncharacterized protein OS=Brucella phage EF4 OX=2706778 PE=4 SV=1
MM1 pKa = 7.98VDD3 pKa = 3.59IRR5 pKa = 11.84PKK7 pKa = 10.53GLPPAILPLRR17 pKa = 11.84TGDD20 pKa = 4.89AVIIDD25 pKa = 3.66QGADD29 pKa = 3.12GVRR32 pKa = 11.84QTNPPRR38 pKa = 11.84MAIDD42 pKa = 3.36MRR44 pKa = 11.84EE45 pKa = 3.96KK46 pKa = 10.14RR47 pKa = 11.84WIVILKK53 pKa = 7.84YY54 pKa = 10.01HH55 pKa = 6.18SKK57 pKa = 10.8RR58 pKa = 11.84FF59 pKa = 3.55

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

11143

39

774

154.8

17.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.261 ± 0.437

1.238 ± 0.165

5.878 ± 0.283

6.811 ± 0.459

3.195 ± 0.175

7.817 ± 0.456

1.777 ± 0.15

5.645 ± 0.268

5.609 ± 0.443

7.691 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.944 ± 0.151

4.353 ± 0.336

4.963 ± 0.3

4.308 ± 0.414

5.761 ± 0.249

6.74 ± 0.241

5.079 ± 0.254

6.111 ± 0.223

1.624 ± 0.192

3.195 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski