Olive viral satellite RNA
Average proteome isoelectric point is 9.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V5J7G1|V5J7G1_9VIRU Coat protein OS=Olive viral satellite RNA OX=1250316 PE=4 SV=1
MM1 pKa = 7.66 ARR3 pKa = 11.84 GRR5 pKa = 11.84 RR6 pKa = 11.84 SNRR9 pKa = 11.84 RR10 pKa = 11.84 TRR12 pKa = 11.84 RR13 pKa = 11.84 AAPRR17 pKa = 11.84 RR18 pKa = 11.84 NPAAITDD25 pKa = 3.99 MYY27 pKa = 10.84 FCTVPSASTQALNRR41 pKa = 11.84 DD42 pKa = 3.62 TFPILKK48 pKa = 10.54 SMGDD52 pKa = 3.19 RR53 pKa = 11.84 PFRR56 pKa = 11.84 VAGISIEE63 pKa = 4.14 YY64 pKa = 10.27 SSPLPCLVQFRR75 pKa = 11.84 CYY77 pKa = 10.02 GPSMTDD83 pKa = 3.36 NINSSGVLMAGPQPRR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 NLRR103 pKa = 11.84 SRR105 pKa = 11.84 GSWFPGNTAGSTTLAAIDD123 pKa = 3.86 VLRR126 pKa = 11.84 SDD128 pKa = 3.83 TVRR131 pKa = 11.84 AQTDD135 pKa = 3.14 VRR137 pKa = 11.84 ATFVVRR143 pKa = 11.84 IRR145 pKa = 11.84 FFVAPPEE152 pKa = 4.24 LQSRR156 pKa = 4.14
Molecular weight: 17.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.133
IPC2_protein 10.145
IPC_protein 11.579
Toseland 11.711
ProMoST 12.208
Dawson 11.711
Bjellqvist 11.711
Wikipedia 12.179
Rodwell 11.257
Grimsley 11.754
Solomon 12.208
Lehninger 12.106
Nozaki 11.711
DTASelect 11.711
Thurlkill 11.711
EMBOSS 12.208
Sillero 11.711
Patrickios 10.994
IPC_peptide 12.208
IPC2_peptide 11.199
IPC2.peptide.svr19 9.578
Protein with the highest isoelectric point:
>tr|V5J7G1|V5J7G1_9VIRU Coat protein OS=Olive viral satellite RNA OX=1250316 PE=4 SV=1
MM1 pKa = 7.66 ARR3 pKa = 11.84 GRR5 pKa = 11.84 RR6 pKa = 11.84 SNRR9 pKa = 11.84 RR10 pKa = 11.84 TRR12 pKa = 11.84 RR13 pKa = 11.84 AAPRR17 pKa = 11.84 RR18 pKa = 11.84 NPAAITDD25 pKa = 3.99 MYY27 pKa = 10.84 FCTVPSASTQALNRR41 pKa = 11.84 DD42 pKa = 3.62 TFPILKK48 pKa = 10.54 SMGDD52 pKa = 3.19 RR53 pKa = 11.84 PFRR56 pKa = 11.84 VAGISIEE63 pKa = 4.14 YY64 pKa = 10.27 SSPLPCLVQFRR75 pKa = 11.84 CYY77 pKa = 10.02 GPSMTDD83 pKa = 3.36 NINSSGVLMAGPQPRR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 NLRR103 pKa = 11.84 SRR105 pKa = 11.84 GSWFPGNTAGSTTLAAIDD123 pKa = 3.86 VLRR126 pKa = 11.84 SDD128 pKa = 3.83 TVRR131 pKa = 11.84 AQTDD135 pKa = 3.14 VRR137 pKa = 11.84 ATFVVRR143 pKa = 11.84 IRR145 pKa = 11.84 FFVAPPEE152 pKa = 4.24 LQSRR156 pKa = 4.14
Molecular weight: 17.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.133
IPC2_protein 10.145
IPC_protein 11.579
Toseland 11.711
ProMoST 12.208
Dawson 11.711
Bjellqvist 11.711
Wikipedia 12.179
Rodwell 11.257
Grimsley 11.754
Solomon 12.208
Lehninger 12.106
Nozaki 11.711
DTASelect 11.711
Thurlkill 11.711
EMBOSS 12.208
Sillero 11.711
Patrickios 10.994
IPC_peptide 12.208
IPC2_peptide 11.199
IPC2.peptide.svr19 9.578
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
156
156
156
156.0
17.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.615 ± 0.0
1.923 ± 0.0
4.487 ± 0.0
1.282 ± 0.0
5.128 ± 0.0
5.769 ± 0.0
0.0 ± 0.0
4.487 ± 0.0
0.641 ± 0.0
5.769 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.205 ± 0.0
4.487 ± 0.0
8.333 ± 0.0
3.205 ± 0.0
15.385 ± 0.0
9.615 ± 0.0
7.692 ± 0.0
6.41 ± 0.0
0.641 ± 0.0
1.923 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here