Mycobacterium phage SimranZ1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4GSV7|A0A2H4GSV7_9CAUD Uncharacterized protein OS=Mycobacterium phage SimranZ1 OX=1933771 GN=SimranZ1_44 PE=4 SV=1
MM1 pKa = 7.9GIPNATHH8 pKa = 7.02KK9 pKa = 10.66AASDD13 pKa = 4.4AIAALGDD20 pKa = 4.06WISVHH25 pKa = 5.89TGAAGTTGANEE36 pKa = 4.04ATGGGYY42 pKa = 10.41ARR44 pKa = 11.84EE45 pKa = 4.16QTSWTSGSTGTNTGDD60 pKa = 3.21EE61 pKa = 4.44VEE63 pKa = 4.37ISVAAGTYY71 pKa = 9.77VEE73 pKa = 4.2GGIWSASSSGTFVGSEE89 pKa = 3.96AFDD92 pKa = 4.61DD93 pKa = 4.53GDD95 pKa = 4.18VEE97 pKa = 4.59VSGSGASISVTPRR110 pKa = 11.84IVAA113 pKa = 3.74

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4GSZ0|A0A2H4GSZ0_9CAUD Uncharacterized protein OS=Mycobacterium phage SimranZ1 OX=1933771 GN=SimranZ1_29 PE=4 SV=1
MM1 pKa = 7.29NKK3 pKa = 9.07IHH5 pKa = 6.77IASHH9 pKa = 6.72GPAGWNATILFTAGTVYY26 pKa = 10.11TVADD30 pKa = 3.84DD31 pKa = 3.14QGRR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 6.83LIDD39 pKa = 3.19TSRR42 pKa = 11.84VTVRR46 pKa = 11.84RR47 pKa = 11.84LSS49 pKa = 3.31

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

17861

28

1175

173.4

18.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.811 ± 0.559

1.17 ± 0.18

6.539 ± 0.224

5.817 ± 0.321

2.788 ± 0.213

8.807 ± 0.645

2.223 ± 0.184

4.261 ± 0.183

3.354 ± 0.198

7.693 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.12

3.314 ± 0.182

6.008 ± 0.187

3.449 ± 0.218

6.679 ± 0.356

6.192 ± 0.279

6.668 ± 0.274

7.127 ± 0.264

2.228 ± 0.143

2.648 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski