Fusarium graminearum mycovirus China 9
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7DDJ4|E7DDJ4_9VIRU Uncharacterized protein OS=Fusarium graminearum mycovirus China 9 OX=941336 PE=4 SV=2
MM1 pKa = 7.07 TTTFWKK7 pKa = 8.96 STEE10 pKa = 4.26 STDD13 pKa = 4.18 SNSDD17 pKa = 3.34 LAEE20 pKa = 3.7 QRR22 pKa = 11.84 AVHH25 pKa = 4.73 STEE28 pKa = 3.93 YY29 pKa = 10.57 AVCTIHH35 pKa = 6.5 QAWGLPSTPYY45 pKa = 10.94 SLGGKK50 pKa = 8.04 PDD52 pKa = 2.97 QWEE55 pKa = 4.26 FTTKK59 pKa = 10.84 DD60 pKa = 3.39 EE61 pKa = 4.76 GSGPSKK67 pKa = 10.14 LTGNVVDD74 pKa = 4.85 GFGTLFSDD82 pKa = 4.75 RR83 pKa = 11.84 LFNLPSKK90 pKa = 10.91 RR91 pKa = 11.84 EE92 pKa = 3.83 VTFFLHH98 pKa = 6.7 SDD100 pKa = 3.29 TAIEE104 pKa = 3.8 MRR106 pKa = 11.84 IINSNTFDD114 pKa = 3.93 RR115 pKa = 11.84 LEE117 pKa = 4.11 NSCICVEE124 pKa = 3.99 VCIVSRR130 pKa = 11.84 ASPVQKK136 pKa = 10.07 HH137 pKa = 5.37 RR138 pKa = 11.84 PVVLHH143 pKa = 5.81 FWVYY147 pKa = 11.5 DD148 pKa = 3.74 EE149 pKa = 5.17 FSGGQVKK156 pKa = 10.07 IVLVEE161 pKa = 3.68 TAYY164 pKa = 10.65 DD165 pKa = 3.49 YY166 pKa = 11.78 SPFLRR171 pKa = 11.84 IDD173 pKa = 3.18 RR174 pKa = 11.84 SGIGAAIRR182 pKa = 11.84 SITSIHH188 pKa = 6.03 TLGQTGTLNGSNVFSVVPWDD208 pKa = 3.68 ANKK211 pKa = 10.35 LQSCSVKK218 pKa = 10.26 DD219 pKa = 3.56 LRR221 pKa = 11.84 RR222 pKa = 11.84 LFVSTSNGKK231 pKa = 6.08 TAKK234 pKa = 10.23 LKK236 pKa = 10.91 APLDD240 pKa = 3.56 IKK242 pKa = 10.76 QAAEE246 pKa = 4.21 YY247 pKa = 9.68 PLLIYY252 pKa = 10.75 LGTSNDD258 pKa = 3.6 HH259 pKa = 6.36 EE260 pKa = 4.53 NATRR264 pKa = 11.84 MVLLRR269 pKa = 11.84 RR270 pKa = 11.84 RR271 pKa = 11.84 KK272 pKa = 8.62 DD273 pKa = 3.52 TPDD276 pKa = 3.32 TLCEE280 pKa = 3.9 VLAQLLVRR288 pKa = 11.84 ARR290 pKa = 11.84 RR291 pKa = 11.84 CPDD294 pKa = 2.95 SQRR297 pKa = 11.84 CDD299 pKa = 3.65 DD300 pKa = 4.89 ANDD303 pKa = 3.86 PSAMLPSNVLVLATNGGKK321 pKa = 9.4 QVTATCVAVRR331 pKa = 11.84 VRR333 pKa = 11.84 ADD335 pKa = 2.88 GRR337 pKa = 11.84 YY338 pKa = 9.44 LYY340 pKa = 10.1 STMRR344 pKa = 11.84 RR345 pKa = 11.84 NCLPYY350 pKa = 8.93 TDD352 pKa = 3.84 QVKK355 pKa = 8.38 FTSIVPSAHH364 pKa = 5.58 NLIMEE369 pKa = 4.58 RR370 pKa = 11.84 SFAVDD375 pKa = 2.57 NWGSIMRR382 pKa = 11.84 VNTNNSPVVVHH393 pKa = 5.83 TAVTEE398 pKa = 4.38 VIRR401 pKa = 11.84 NAALGTTDD409 pKa = 2.85 TTVYY413 pKa = 9.98 VRR415 pKa = 11.84 HH416 pKa = 5.92 VLDD419 pKa = 4.41 AFGTICSLMDD429 pKa = 3.23 RR430 pKa = 11.84 TVGLSYY436 pKa = 9.91 EE437 pKa = 4.16 IPQDD441 pKa = 3.84 KK442 pKa = 10.41 NFCQIRR448 pKa = 11.84 WKK450 pKa = 10.7 HH451 pKa = 6.05 DD452 pKa = 3.42 ALSLKK457 pKa = 9.04 GTLFIASAVNPGTCLLGVLGSWQNWAGPGSGGGQIPQSQEE497 pKa = 2.91 YY498 pKa = 8.56 WYY500 pKa = 9.55 PIDD503 pKa = 3.69 GRR505 pKa = 11.84 GGKK508 pKa = 9.27 DD509 pKa = 2.95 CYY511 pKa = 11.17 GEE513 pKa = 5.11 LMYY516 pKa = 10.97 LDD518 pKa = 4.89 GGFSLLGRR526 pKa = 11.84 YY527 pKa = 9.1 LYY529 pKa = 10.62 QDD531 pKa = 4.34 VINSGQYY538 pKa = 10.99 LDD540 pKa = 3.93 LTSTLVPKK548 pKa = 10.65 ALRR551 pKa = 11.84 DD552 pKa = 3.82 DD553 pKa = 4.75 GLITDD558 pKa = 4.01 NLRR561 pKa = 11.84 VYY563 pKa = 10.37 VGPHH567 pKa = 6.27 EE568 pKa = 4.35 GDD570 pKa = 3.09 KK571 pKa = 11.34 VNFMLDD577 pKa = 2.86 IEE579 pKa = 4.56 YY580 pKa = 10.31 TDD582 pKa = 5.02 LPGGDD587 pKa = 3.99 SKK589 pKa = 10.32 TPTAIGYY596 pKa = 8.09 CAVQQGEE603 pKa = 4.55 SLSSGLSTMHH613 pKa = 6.57 ATTLDD618 pKa = 3.79 GYY620 pKa = 11.38 GLITDD625 pKa = 4.75 PKK627 pKa = 10.55 SSAKK631 pKa = 10.14 LDD633 pKa = 3.27 QTLQQRR639 pKa = 11.84 VGKK642 pKa = 9.58 HH643 pKa = 4.22 RR644 pKa = 11.84 QLSKK648 pKa = 10.78 RR649 pKa = 11.84 YY650 pKa = 9.14 ASSNLWEE657 pKa = 4.96 DD658 pKa = 3.58 LAVLQTLALSSNSVQLWAKK677 pKa = 10.3 GADD680 pKa = 3.25 NDD682 pKa = 4.81 AAFLAAATPVNSSSCPTWNSIKK704 pKa = 10.64 HH705 pKa = 5.44 EE706 pKa = 4.26 GAGLVANGTLLDD718 pKa = 4.16 IGGEE722 pKa = 4.14 LPKK725 pKa = 10.42 FAALAKK731 pKa = 8.3 RR732 pKa = 11.84 HH733 pKa = 5.65 KK734 pKa = 10.51 VSSEE738 pKa = 3.77 HH739 pKa = 6.0 NPCIEE744 pKa = 4.05 SALFAIEE751 pKa = 3.63 AGIWRR756 pKa = 11.84 KK757 pKa = 7.6 TPDD760 pKa = 3.52 LASVRR765 pKa = 11.84 LSTTSLAEE773 pKa = 4.03 GLADD777 pKa = 4.62 LLNRR781 pKa = 11.84 LYY783 pKa = 10.29 TQDD786 pKa = 3.71 VLIKK790 pKa = 10.62 GDD792 pKa = 5.35 DD793 pKa = 3.55 GAEE796 pKa = 3.82 WVEE799 pKa = 4.74 GITGTFDD806 pKa = 2.85 IHH808 pKa = 7.19 VISPDD813 pKa = 3.15 HH814 pKa = 6.86 PARR817 pKa = 11.84 EE818 pKa = 4.2 ALRR821 pKa = 11.84 IVTDD825 pKa = 3.73 RR826 pKa = 11.84 VIAASKK832 pKa = 10.65 DD833 pKa = 3.8 SNN835 pKa = 3.61
Molecular weight: 91.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.79
IPC2_protein 5.753
IPC_protein 5.817
Toseland 6.173
ProMoST 6.198
Dawson 6.109
Bjellqvist 6.122
Wikipedia 6.122
Rodwell 6.097
Grimsley 6.287
Solomon 6.109
Lehninger 6.109
Nozaki 6.389
DTASelect 6.576
Thurlkill 6.59
EMBOSS 6.547
Sillero 6.491
Patrickios 4.088
IPC_peptide 6.122
IPC2_peptide 6.465
IPC2.peptide.svr19 6.436
Protein with the highest isoelectric point:
>tr|E7DDJ2|E7DDJ2_9VIRU Putative coat protein OS=Fusarium graminearum mycovirus China 9 OX=941336 PE=4 SV=1
MM1 pKa = 7.6 SGQLNIRR8 pKa = 11.84 STAHH12 pKa = 7.2 LEE14 pKa = 4.16 QQAVAPDD21 pKa = 3.56 QYY23 pKa = 11.13 RR24 pKa = 11.84 AGAVAGLCSYY34 pKa = 10.38 SVPTPSITRR43 pKa = 11.84 RR44 pKa = 11.84 VHH46 pKa = 5.9 YY47 pKa = 9.83 NAGVSTVEE55 pKa = 4.21 SGAGSADD62 pKa = 3.31 KK63 pKa = 11.29 LEE65 pKa = 4.34 SFYY68 pKa = 11.14 HH69 pKa = 5.53 HH70 pKa = 7.5 RR71 pKa = 11.84 IPRR74 pKa = 11.84 DD75 pKa = 3.24 VALTSLRR82 pKa = 11.84 AGTIKK87 pKa = 10.52 HH88 pKa = 6.39 DD89 pKa = 3.7 VVTFDD94 pKa = 3.12 VSEE97 pKa = 4.6 RR98 pKa = 11.84 GPTQPLTNQIVNVTYY113 pKa = 9.56 KK114 pKa = 9.89 QQYY117 pKa = 8.21 NFIDD121 pKa = 3.72 VGVSAAIVRR130 pKa = 11.84 TEE132 pKa = 3.8 LQFFPVRR139 pKa = 11.84 PPVDD143 pKa = 3.4 NTKK146 pKa = 11.15 LNLLKK151 pKa = 10.77 NALTVHH157 pKa = 6.01 EE158 pKa = 4.5 VVKK161 pKa = 10.05 PLKK164 pKa = 10.39 LQLPKK169 pKa = 10.72 LVAQSHH175 pKa = 6.69 ASPGAQLYY183 pKa = 11.02 SDD185 pKa = 4.59 FEE187 pKa = 4.19 PLRR190 pKa = 11.84 RR191 pKa = 11.84 AIANQAINDD200 pKa = 4.06 DD201 pKa = 3.58 YY202 pKa = 11.95 GMRR205 pKa = 11.84 LSHH208 pKa = 6.31 MARR211 pKa = 11.84 IVLHH215 pKa = 6.51 SFTAQAAGLSHH226 pKa = 7.07 AASDD230 pKa = 4.0 VVSTVDD236 pKa = 3.14 VRR238 pKa = 11.84 TMFVAEE244 pKa = 4.98 TYY246 pKa = 10.84 QMSAAEE252 pKa = 4.0 LQQRR256 pKa = 11.84 AFVYY260 pKa = 10.69 VDD262 pKa = 3.74 DD263 pKa = 6.3 DD264 pKa = 3.51 ISEE267 pKa = 4.54 EE268 pKa = 4.17 YY269 pKa = 9.63 WAFLAMAMLGINTNVGNTRR288 pKa = 11.84 TVYY291 pKa = 10.51 SRR293 pKa = 11.84 LVSVSEE299 pKa = 4.07 LRR301 pKa = 11.84 GGQQLYY307 pKa = 10.02 YY308 pKa = 10.97 VMKK311 pKa = 10.56 SGEE314 pKa = 4.13 NTPANISQNACASVLNNPEE333 pKa = 4.03 KK334 pKa = 10.9 CLSFYY339 pKa = 10.91 NAYY342 pKa = 9.96 AQSLGLAQEE351 pKa = 4.25 ASSVLRR357 pKa = 11.84 QVAVMPFMFTKK368 pKa = 10.65 NATLPYY374 pKa = 9.63 KK375 pKa = 10.66 ARR377 pKa = 11.84 EE378 pKa = 4.03 NPKK381 pKa = 10.04 ADD383 pKa = 3.04 AYY385 pKa = 11.25 VYY387 pKa = 10.66 LLRR390 pKa = 11.84 EE391 pKa = 3.86 TAEE394 pKa = 5.24 DD395 pKa = 3.11 IRR397 pKa = 11.84 LMYY400 pKa = 10.73 NSVEE404 pKa = 4.18 TLVYY408 pKa = 9.06 QAPIIAGAVKK418 pKa = 10.42 AGVAAISMSYY428 pKa = 9.87 RR429 pKa = 11.84 VKK431 pKa = 11.03 NSLNVPSILAAIKK444 pKa = 10.13 EE445 pKa = 4.14 ILADD449 pKa = 3.9 PSSCRR454 pKa = 11.84 STMSAVLCSYY464 pKa = 11.28 GGITADD470 pKa = 5.46 LEE472 pKa = 4.11 WLLPFTEE479 pKa = 4.83 GVDD482 pKa = 3.26 IAMDD486 pKa = 3.69 ACVRR490 pKa = 11.84 AYY492 pKa = 10.33 RR493 pKa = 11.84 DD494 pKa = 4.09 CGWLITTYY502 pKa = 11.4 NDD504 pKa = 3.97 LSCLGAYY511 pKa = 9.91 APAFSTGVDD520 pKa = 3.2 MRR522 pKa = 11.84 NAQFGYY528 pKa = 10.67 DD529 pKa = 3.62 VASRR533 pKa = 11.84 PYY535 pKa = 10.53 LEE537 pKa = 4.79 SLIALSTQGLPWTFEE552 pKa = 4.07 NEE554 pKa = 4.35 SLDD557 pKa = 3.88 GPTLQRR563 pKa = 11.84 AARR566 pKa = 11.84 DD567 pKa = 3.86 VVHH570 pKa = 6.6 WEE572 pKa = 5.15 AVIQWDD578 pKa = 4.14 AYY580 pKa = 10.79 SPGEE584 pKa = 4.28 GVSVAHH590 pKa = 6.2 ITPTPVSPKK599 pKa = 7.73 QARR602 pKa = 11.84 PFEE605 pKa = 4.45 TEE607 pKa = 3.81 PNAHH611 pKa = 6.98 LSEE614 pKa = 4.5 RR615 pKa = 11.84 WQKK618 pKa = 10.53 SKK620 pKa = 10.22 RR621 pKa = 11.84 TLSGNGLKK629 pKa = 10.51 ALTLSPPSAAARR641 pKa = 11.84 SEE643 pKa = 4.03 ATKK646 pKa = 9.18 TASVAEE652 pKa = 4.23 TLRR655 pKa = 11.84 THH657 pKa = 5.38 TRR659 pKa = 11.84 SMSVKK664 pKa = 9.59 PNPAPGTKK672 pKa = 8.63 PHH674 pKa = 6.01 NMPWPVQAAASVPSGEE690 pKa = 4.39 VSDD693 pKa = 4.97 SEE695 pKa = 4.6 STTTRR700 pKa = 11.84 VTRR703 pKa = 11.84 ANSVASTDD711 pKa = 3.81 DD712 pKa = 3.34 TASAATVVQTAPPGVTSPPRR732 pKa = 11.84 GRR734 pKa = 11.84 SVTPIGTTVDD744 pKa = 3.93 LSRR747 pKa = 11.84 EE748 pKa = 4.19 PTPSLAEE755 pKa = 3.88 RR756 pKa = 11.84 LKK758 pKa = 11.19 SPFRR762 pKa = 11.84 HH763 pKa = 4.36 QRR765 pKa = 11.84 VRR767 pKa = 11.84 SRR769 pKa = 11.84 SVSSSRR775 pKa = 11.84 GRR777 pKa = 11.84 ATSDD781 pKa = 2.84 TNMGQMLDD789 pKa = 3.47 RR790 pKa = 11.84 GASEE794 pKa = 4.01 SSEE797 pKa = 3.45 IALIRR802 pKa = 11.84 KK803 pKa = 7.0 TKK805 pKa = 9.87 PKK807 pKa = 10.5 DD808 pKa = 3.37 VAEE811 pKa = 4.48 LGEE814 pKa = 4.87 HH815 pKa = 6.33 IVTSDD820 pKa = 3.17 GRR822 pKa = 11.84 IEE824 pKa = 6.35 LITQLAGKK832 pKa = 9.54 RR833 pKa = 11.84 DD834 pKa = 3.76 YY835 pKa = 11.65 SDD837 pKa = 3.29 VVRR840 pKa = 11.84 DD841 pKa = 3.95 LLPGGVGTPDD851 pKa = 2.95 VGDD854 pKa = 3.29 WTGTSKK860 pKa = 10.96 NGEE863 pKa = 3.91 QRR865 pKa = 11.84 NITTLMNVNKK875 pKa = 10.52
Molecular weight: 94.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.853
IPC2_protein 7.161
IPC_protein 7.146
Toseland 7.044
ProMoST 7.761
Dawson 7.775
Bjellqvist 7.98
Wikipedia 7.717
Rodwell 7.775
Grimsley 7.146
Solomon 7.834
Lehninger 7.849
Nozaki 8.126
DTASelect 7.922
Thurlkill 7.966
EMBOSS 8.024
Sillero 8.2
Patrickios 4.329
IPC_peptide 7.834
IPC2_peptide 7.293
IPC2.peptide.svr19 7.244
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
4556
835
1137
911.2
100.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.206 ± 0.559
2.019 ± 0.68
5.948 ± 0.366
5.114 ± 0.472
2.788 ± 0.212
6.255 ± 0.676
3.622 ± 1.152
3.512 ± 0.4
3.775 ± 0.342
8.275 ± 0.457
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.195 ± 0.33
3.929 ± 0.275
4.587 ± 0.434
4.368 ± 0.372
6.738 ± 0.383
8.033 ± 0.541
6.497 ± 0.421
7.309 ± 0.599
1.449 ± 0.146
3.38 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here