Blacklegged tick phlebovirus 3
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A172MHV7|A0A172MHV7_9VIRU Nucleocapsid protein OS=Blacklegged tick phlebovirus 3 OX=1844920 PE=4 SV=1
MM1 pKa = 6.05 MTQVKK6 pKa = 8.97 TEE8 pKa = 3.93 EE9 pKa = 4.51 GLTKK13 pKa = 10.52 ALSATMEE20 pKa = 4.21 KK21 pKa = 10.84 AEE23 pKa = 4.73 AAIIDD28 pKa = 4.17 TNFVSARR35 pKa = 11.84 TYY37 pKa = 8.84 LTYY40 pKa = 10.93 VQDD43 pKa = 4.4 CHH45 pKa = 7.25 DD46 pKa = 3.49 IWVKK50 pKa = 10.48 RR51 pKa = 11.84 GNPSVPDD58 pKa = 3.48 INVWLANTRR67 pKa = 11.84 ILIPEE72 pKa = 4.12 EE73 pKa = 3.95 TSSDD77 pKa = 3.62 EE78 pKa = 3.98 EE79 pKa = 4.13 GAEE82 pKa = 4.18 VVEE85 pKa = 4.78 VPPAPARR92 pKa = 11.84 VATPKK97 pKa = 10.28 RR98 pKa = 11.84 KK99 pKa = 10.16 AMDD102 pKa = 3.53 QDD104 pKa = 3.76 QEE106 pKa = 4.33 AGPSGVPPQKK116 pKa = 10.18 RR117 pKa = 11.84 AATLEE122 pKa = 3.9 EE123 pKa = 4.13 VLRR126 pKa = 11.84 VLEE129 pKa = 4.56 IAPEE133 pKa = 4.22 TPPGPDD139 pKa = 2.73 EE140 pKa = 4.55 VNWRR144 pKa = 11.84 QALDD148 pKa = 3.63 TLDD151 pKa = 3.75 MEE153 pKa = 5.2 GKK155 pKa = 9.91 NIHH158 pKa = 5.67 VLQEE162 pKa = 4.03 LVQLFQFRR170 pKa = 11.84 GFNAGKK176 pKa = 9.4 LAKK179 pKa = 9.89 HH180 pKa = 5.94 IMGKK184 pKa = 8.69 EE185 pKa = 3.48 PCGYY189 pKa = 9.43 FKK191 pKa = 10.9 KK192 pKa = 10.81 VVGGVATPDD201 pKa = 2.93 KK202 pKa = 11.12 TMDD205 pKa = 3.37 ILAMVIIGMSRR216 pKa = 11.84 GSKK219 pKa = 9.36 IEE221 pKa = 4.11 KK222 pKa = 8.97 IRR224 pKa = 11.84 KK225 pKa = 8.65 GLSEE229 pKa = 5.19 DD230 pKa = 3.21 GLTAVNSLITAYY242 pKa = 10.29 GVVSTTRR249 pKa = 11.84 EE250 pKa = 4.07 SLGVTFPRR258 pKa = 11.84 VVAVFPSVAMDD269 pKa = 3.73 IAAQLTMGPVALMTMNSRR287 pKa = 11.84 SPGYY291 pKa = 8.87 PRR293 pKa = 11.84 CLMCSGLPSLIPTTTAEE310 pKa = 4.17 YY311 pKa = 11.28 AEE313 pKa = 4.53 VVLNAYY319 pKa = 10.11 LLYY322 pKa = 10.17 QLEE325 pKa = 4.37 VSLILNPDD333 pKa = 3.7 FKK335 pKa = 10.86 KK336 pKa = 10.56 QSRR339 pKa = 11.84 EE340 pKa = 3.77 EE341 pKa = 3.79 QKK343 pKa = 9.7 RR344 pKa = 11.84 TVEE347 pKa = 4.08 GFARR351 pKa = 11.84 AAMQSSYY358 pKa = 10.89 CSEE361 pKa = 4.07 EE362 pKa = 3.54 QRR364 pKa = 11.84 IRR366 pKa = 11.84 QMLKK370 pKa = 9.15 HH371 pKa = 6.19 EE372 pKa = 4.29 LVQVEE377 pKa = 4.64 VTGLRR382 pKa = 11.84 APDD385 pKa = 3.76 TNLGRR390 pKa = 11.84 ALLNAAAEE398 pKa = 4.2 YY399 pKa = 10.3 RR400 pKa = 11.84 RR401 pKa = 11.84 YY402 pKa = 10.49
Molecular weight: 44.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.557
IPC2_protein 5.677
IPC_protein 5.639
Toseland 5.753
ProMoST 5.855
Dawson 5.728
Bjellqvist 5.804
Wikipedia 5.639
Rodwell 5.69
Grimsley 5.766
Solomon 5.728
Lehninger 5.703
Nozaki 5.906
DTASelect 6.046
Thurlkill 5.919
EMBOSS 5.855
Sillero 6.008
Patrickios 4.38
IPC_peptide 5.741
IPC2_peptide 5.982
IPC2.peptide.svr19 6.123
Protein with the highest isoelectric point:
>tr|A0A172MHV7|A0A172MHV7_9VIRU Nucleocapsid protein OS=Blacklegged tick phlebovirus 3 OX=1844920 PE=4 SV=1
MM1 pKa = 7.79 EE2 pKa = 4.89 ILEE5 pKa = 4.16 RR6 pKa = 11.84 KK7 pKa = 9.29 ALNKK11 pKa = 9.57 NPNMTHH17 pKa = 7.09 ASRR20 pKa = 11.84 LEE22 pKa = 3.86 ALTEE26 pKa = 4.0 VLLSEE31 pKa = 4.8 EE32 pKa = 4.38 KK33 pKa = 10.62 FLTPGLHH40 pKa = 6.36 SPLLEE45 pKa = 4.1 VFEE48 pKa = 4.36 SEE50 pKa = 3.98 RR51 pKa = 11.84 WEE53 pKa = 4.17 AQPKK57 pKa = 8.46 VQVSVEE63 pKa = 4.06 GSHH66 pKa = 7.26 DD67 pKa = 3.72 LRR69 pKa = 11.84 LEE71 pKa = 4.3 FPDD74 pKa = 3.6 LEE76 pKa = 4.24 EE77 pKa = 4.06 WRR79 pKa = 11.84 GSSRR83 pKa = 11.84 MSAMSVVNQVWPQVKK98 pKa = 9.2 MSNVRR103 pKa = 11.84 HH104 pKa = 7.11 DD105 pKa = 3.62 YY106 pKa = 9.64 TAGLFSRR113 pKa = 11.84 NLDD116 pKa = 3.51 RR117 pKa = 11.84 PLKK120 pKa = 10.64 SFFKK124 pKa = 10.31 PLHH127 pKa = 6.16 DD128 pKa = 5.42 HH129 pKa = 7.17 NDD131 pKa = 3.53 DD132 pKa = 3.69 LTPDD136 pKa = 4.2 VIQEE140 pKa = 3.97 RR141 pKa = 11.84 NGIILVHH148 pKa = 6.3 EE149 pKa = 4.92 FATTSIPDD157 pKa = 3.48 INRR160 pKa = 11.84 MKK162 pKa = 10.98 EE163 pKa = 3.94 MFHH166 pKa = 6.05 MKK168 pKa = 9.99 HH169 pKa = 5.9 LRR171 pKa = 11.84 YY172 pKa = 10.21 DD173 pKa = 3.52 SALSARR179 pKa = 11.84 AEE181 pKa = 4.31 KK182 pKa = 10.37 EE183 pKa = 3.82 GKK185 pKa = 7.83 TVIFSVTVVGPRR197 pKa = 11.84 LVVSSLPLTQEE208 pKa = 4.58 DD209 pKa = 4.17 VTEE212 pKa = 4.01 LCIRR216 pKa = 11.84 LLIGRR221 pKa = 11.84 QIEE224 pKa = 4.1 FSLFSQRR231 pKa = 11.84 VIAHH235 pKa = 6.33 PAEE238 pKa = 4.49 KK239 pKa = 10.32 EE240 pKa = 3.82 EE241 pKa = 4.22 SDD243 pKa = 3.97 RR244 pKa = 11.84 IQAARR249 pKa = 11.84 VAFSQIGHH257 pKa = 6.05 DD258 pKa = 3.65 WEE260 pKa = 4.4 RR261 pKa = 11.84 LNSEE265 pKa = 4.73 FPSCSQEE272 pKa = 3.77 VYY274 pKa = 9.79 EE275 pKa = 4.8 ASKK278 pKa = 10.71 EE279 pKa = 4.16 KK280 pKa = 10.29 PDD282 pKa = 3.64 LKK284 pKa = 11.09 YY285 pKa = 9.84 ITEE288 pKa = 4.05 QLQAASIKK296 pKa = 10.36 ARR298 pKa = 11.84 QKK300 pKa = 10.17 LRR302 pKa = 11.84 KK303 pKa = 8.52 EE304 pKa = 3.89 HH305 pKa = 6.46 FLGEE309 pKa = 4.16 EE310 pKa = 4.25 HH311 pKa = 7.16 PSSISDD317 pKa = 3.41 RR318 pKa = 11.84 LKK320 pKa = 11.0 LNQEE324 pKa = 3.78 EE325 pKa = 4.7 CVAEE329 pKa = 3.91 INKK332 pKa = 9.58 YY333 pKa = 10.77 LEE335 pKa = 4.56 LNEE338 pKa = 4.42 PPEE341 pKa = 4.09 PQLIYY346 pKa = 9.11 NTKK349 pKa = 10.37 SVVQIPWWIPEE360 pKa = 4.15 RR361 pKa = 11.84 GTGSNSLSIEE371 pKa = 3.71 RR372 pKa = 11.84 SPGNEE377 pKa = 4.01 CLPDD381 pKa = 4.09 IGEE384 pKa = 4.36 SKK386 pKa = 10.94 EE387 pKa = 3.96 DD388 pKa = 3.47 TFLCWRR394 pKa = 11.84 SAWDD398 pKa = 3.46 YY399 pKa = 9.16 ATQHH403 pKa = 6.67 PEE405 pKa = 3.48 HH406 pKa = 6.14 MTEE409 pKa = 3.66 EE410 pKa = 4.51 TIDD413 pKa = 3.76 VEE415 pKa = 4.26 IARR418 pKa = 11.84 AMTEE422 pKa = 4.04 TPEE425 pKa = 4.14 EE426 pKa = 3.98 EE427 pKa = 4.32 VEE429 pKa = 4.91 LSEE432 pKa = 4.54 EE433 pKa = 4.26 QQKK436 pKa = 10.07 HH437 pKa = 3.89 AAAVKK442 pKa = 9.62 LQTAGVLEE450 pKa = 4.26 KK451 pKa = 10.11 TPMGMRR457 pKa = 11.84 SKK459 pKa = 10.56 YY460 pKa = 10.11 RR461 pKa = 11.84 RR462 pKa = 11.84 VQYY465 pKa = 8.93 EE466 pKa = 3.98 VPYY469 pKa = 7.45 WTSVEE474 pKa = 3.9 LAKK477 pKa = 10.66 RR478 pKa = 11.84 GIEE481 pKa = 4.05 GKK483 pKa = 9.95 KK484 pKa = 10.18 RR485 pKa = 11.84 RR486 pKa = 11.84 DD487 pKa = 3.46 EE488 pKa = 4.57 PEE490 pKa = 4.0 VKK492 pKa = 10.03 AHH494 pKa = 5.71 KK495 pKa = 9.87 QRR497 pKa = 11.84 SKK499 pKa = 11.22 RR500 pKa = 11.84 PMSYY504 pKa = 8.39 KK505 pKa = 10.31 TEE507 pKa = 4.02 VADD510 pKa = 5.26 LDD512 pKa = 4.02 KK513 pKa = 11.43 LLDD516 pKa = 3.56 RR517 pKa = 11.84 AQRR520 pKa = 11.84 IFTNTYY526 pKa = 7.55 SFKK529 pKa = 10.11 GASYY533 pKa = 10.24 DD534 pKa = 3.46 EE535 pKa = 5.12 HH536 pKa = 6.66 MLPDD540 pKa = 4.14 LAGYY544 pKa = 10.11 AYY546 pKa = 9.74 NLHH549 pKa = 5.86 GRR551 pKa = 11.84 GVGEE555 pKa = 3.85 PWKK558 pKa = 10.76 KK559 pKa = 9.93 FLKK562 pKa = 10.75 SFLGCHH568 pKa = 5.91 LGKK571 pKa = 9.9 WSSFVSDD578 pKa = 4.74 LATEE582 pKa = 4.58 LAISMKK588 pKa = 7.93 QHH590 pKa = 6.17 CKK592 pKa = 9.86 SGQMLLKK599 pKa = 10.26 KK600 pKa = 10.11 LRR602 pKa = 11.84 HH603 pKa = 5.64 FDD605 pKa = 3.45 VYY607 pKa = 11.52 VLIKK611 pKa = 8.43 PTNSSGSIFYY621 pKa = 11.04 SLLTFKK627 pKa = 10.87 AGLSVDD633 pKa = 3.93 PLSGGTVFKK642 pKa = 10.94 QDD644 pKa = 3.3 FEE646 pKa = 5.44 NNVVRR651 pKa = 11.84 WTEE654 pKa = 3.97 FNSVSASKK662 pKa = 10.85 LMNLVKK668 pKa = 10.5 CKK670 pKa = 10.05 STMFTMLAYY679 pKa = 9.4 WMEE682 pKa = 4.09 FFGLKK687 pKa = 9.71 FWEE690 pKa = 4.69 HH691 pKa = 6.26 EE692 pKa = 4.25 LNEE695 pKa = 5.24 DD696 pKa = 3.42 SDD698 pKa = 3.73 QMRR701 pKa = 11.84 EE702 pKa = 3.75 VWRR705 pKa = 11.84 MVAVCLMVSLEE716 pKa = 4.43 DD717 pKa = 3.36 KK718 pKa = 11.08 AKK720 pKa = 10.15 AEE722 pKa = 4.34 EE723 pKa = 4.76 IITVSRR729 pKa = 11.84 FVYY732 pKa = 10.04 MEE734 pKa = 4.04 GLVAQPALPKK744 pKa = 9.09 PHH746 pKa = 6.99 KK747 pKa = 10.51 VLGKK751 pKa = 10.47 LPVALRR757 pKa = 11.84 SRR759 pKa = 11.84 LQVWLVGKK767 pKa = 10.39 VLDD770 pKa = 3.72 AMKK773 pKa = 10.3 RR774 pKa = 11.84 ISLRR778 pKa = 11.84 PFDD781 pKa = 5.32 LNSEE785 pKa = 4.12 DD786 pKa = 6.17 RR787 pKa = 11.84 RR788 pKa = 11.84 PKK790 pKa = 9.19 WNFMFNLFTGRR801 pKa = 11.84 VIEE804 pKa = 4.81 EE805 pKa = 3.94 PMQMVSMFYY814 pKa = 9.97 IGYY817 pKa = 9.64 LKK819 pKa = 10.94 NKK821 pKa = 10.06 DD822 pKa = 3.73 EE823 pKa = 4.46 SPQGNSSGALYY834 pKa = 10.48 DD835 pKa = 3.54 KK836 pKa = 11.24 VMEE839 pKa = 4.23 YY840 pKa = 10.76 EE841 pKa = 4.06 SRR843 pKa = 11.84 KK844 pKa = 9.68 PSSNKK849 pKa = 9.69 NLGLGDD855 pKa = 4.37 PLLDD859 pKa = 3.28 DD860 pKa = 4.14 VKK862 pKa = 10.75 FHH864 pKa = 6.26 EE865 pKa = 5.14 FSRR868 pKa = 11.84 SFLSYY873 pKa = 10.81 CVDD876 pKa = 3.59 LSKK879 pKa = 11.47 AKK881 pKa = 9.83 LRR883 pKa = 11.84 KK884 pKa = 8.28 MWGDD888 pKa = 3.42 NVQEE892 pKa = 5.05 LIMDD896 pKa = 5.99 DD897 pKa = 3.17 IMEE900 pKa = 4.45 SLGGYY905 pKa = 6.66 TLEE908 pKa = 4.52 EE909 pKa = 4.75 LGTLKK914 pKa = 10.92 ASATYY919 pKa = 10.05 DD920 pKa = 3.17 QTMYY924 pKa = 10.86 KK925 pKa = 10.18 YY926 pKa = 10.0 DD927 pKa = 3.25 AKK929 pKa = 10.05 KK930 pKa = 8.19 TYY932 pKa = 9.66 RR933 pKa = 11.84 RR934 pKa = 11.84 KK935 pKa = 10.28 KK936 pKa = 9.6 VVEE939 pKa = 4.6 FTAKK943 pKa = 9.94 KK944 pKa = 10.1 AQDD947 pKa = 3.52 FTHH950 pKa = 5.01 VHH952 pKa = 6.39 EE953 pKa = 4.58 IVKK956 pKa = 10.39 EE957 pKa = 3.97 SLEE960 pKa = 4.33 AIEE963 pKa = 5.44 EE964 pKa = 4.16 NGCLHH969 pKa = 7.13 IDD971 pKa = 4.03 LFKK974 pKa = 10.73 KK975 pKa = 9.66 AQHH978 pKa = 5.97 GGLRR982 pKa = 11.84 EE983 pKa = 4.06 IYY985 pKa = 10.43 VLGPNEE991 pKa = 4.25 RR992 pKa = 11.84 VVQLCLEE999 pKa = 4.91 LIARR1003 pKa = 11.84 AICRR1007 pKa = 11.84 RR1008 pKa = 11.84 FPSEE1012 pKa = 4.11 TMMTPANKK1020 pKa = 9.65 FKK1022 pKa = 10.98 LPQQHH1027 pKa = 6.08 NKK1029 pKa = 8.75 NARR1032 pKa = 11.84 KK1033 pKa = 9.54 NCGNNFTTTSTSDD1046 pKa = 4.51 DD1047 pKa = 3.38 ASKK1050 pKa = 10.31 WNQGHH1055 pKa = 5.31 YY1056 pKa = 9.62 VSKK1059 pKa = 10.07 FAMMLCRR1066 pKa = 11.84 FTDD1069 pKa = 4.99 PILHH1073 pKa = 6.16 PFIMRR1078 pKa = 11.84 ACALFTKK1085 pKa = 10.25 KK1086 pKa = 10.39 VIKK1089 pKa = 10.24 IDD1091 pKa = 3.92 DD1092 pKa = 3.82 QLLKK1096 pKa = 11.07 AFVKK1100 pKa = 10.28 HH1101 pKa = 6.79 DD1102 pKa = 3.8 EE1103 pKa = 5.29 DD1104 pKa = 4.21 IFSSPHH1110 pKa = 4.25 VRR1112 pKa = 11.84 KK1113 pKa = 8.51 MHH1115 pKa = 6.38 AAFKK1119 pKa = 10.55 GALGDD1124 pKa = 3.69 AEE1126 pKa = 4.33 YY1127 pKa = 11.38 SHH1129 pKa = 6.97 VEE1131 pKa = 3.64 PGMTFLKK1138 pKa = 9.92 TSTGMLQGILHH1149 pKa = 6.01 YY1150 pKa = 10.62 SSSLLHH1156 pKa = 6.6 TILQEE1161 pKa = 3.86 LMKK1164 pKa = 10.7 DD1165 pKa = 3.8 VIEE1168 pKa = 4.66 GRR1170 pKa = 11.84 LKK1172 pKa = 10.73 RR1173 pKa = 11.84 HH1174 pKa = 5.17 LTLLKK1179 pKa = 10.41 KK1180 pKa = 10.64 SSLKK1184 pKa = 10.59 PYY1186 pKa = 7.67 VTVMQSSDD1194 pKa = 3.7 DD1195 pKa = 3.85 SSIMISFPIDD1205 pKa = 3.08 KK1206 pKa = 10.22 DD1207 pKa = 3.92 RR1208 pKa = 11.84 PEE1210 pKa = 4.45 LTCQGYY1216 pKa = 9.35 ILAWEE1221 pKa = 4.31 SFQMKK1226 pKa = 10.02 KK1227 pKa = 10.75 DD1228 pKa = 3.58 LGLLLGIYY1236 pKa = 9.74 PSEE1239 pKa = 4.32 KK1240 pKa = 9.74 CTTNTPWVTEE1250 pKa = 4.27 FNSEE1254 pKa = 4.19 FFFMSDD1260 pKa = 3.98 LIRR1263 pKa = 11.84 PLFRR1267 pKa = 11.84 WVAAVNTLSEE1277 pKa = 4.45 HH1278 pKa = 5.13 EE1279 pKa = 4.34 TLAGRR1284 pKa = 11.84 QEE1286 pKa = 4.23 EE1287 pKa = 4.43 MSSNLSNVLGGGGTTSLTASCQLAQMMLHH1316 pKa = 5.11 YY1317 pKa = 10.19 QILGSGVSQIFSRR1330 pKa = 11.84 YY1331 pKa = 8.33 VSVLQQDD1338 pKa = 3.65 PSVGFFLLDD1347 pKa = 3.41 HH1348 pKa = 7.04 PFMAGLCGFKK1358 pKa = 10.74 FNLFKK1363 pKa = 10.76 AVKK1366 pKa = 8.39 CTKK1369 pKa = 10.57 LGDD1372 pKa = 3.19 KK1373 pKa = 10.0 YY1374 pKa = 11.02 RR1375 pKa = 11.84 RR1376 pKa = 11.84 VLVTQEE1382 pKa = 4.2 EE1383 pKa = 4.82 VSSDD1387 pKa = 3.05 RR1388 pKa = 11.84 KK1389 pKa = 10.41 AAGLTGKK1396 pKa = 8.73 TMVTTKK1402 pKa = 10.45 AGSIVDD1408 pKa = 3.73 STIVSMSTRR1417 pKa = 11.84 RR1418 pKa = 11.84 RR1419 pKa = 11.84 WQKK1422 pKa = 10.3 LVSGMEE1428 pKa = 4.32 FPADD1432 pKa = 2.77 WRR1434 pKa = 11.84 EE1435 pKa = 3.96 QIKK1438 pKa = 10.38 EE1439 pKa = 4.1 NQEE1442 pKa = 3.67 VLYY1445 pKa = 10.23 EE1446 pKa = 4.07 KK1447 pKa = 10.82 ARR1449 pKa = 11.84 TPEE1452 pKa = 3.86 EE1453 pKa = 4.25 LKK1455 pKa = 11.1 LKK1457 pKa = 10.31 LAAFMHH1463 pKa = 6.18 SPGVVSSLGHH1473 pKa = 5.67 SGAVVRR1479 pKa = 11.84 VMASSAYY1486 pKa = 9.01 CLSHH1490 pKa = 7.03 PVVQDD1495 pKa = 3.11 RR1496 pKa = 11.84 TDD1498 pKa = 3.65 WYY1500 pKa = 11.09 CSLEE1504 pKa = 4.18 TKK1506 pKa = 7.03 TTKK1509 pKa = 10.17 TSLFSLACEE1518 pKa = 4.01 EE1519 pKa = 4.45 SLTEE1523 pKa = 4.8 GGAEE1527 pKa = 4.29 PISDD1531 pKa = 4.06 EE1532 pKa = 3.96 QLLALFPQKK1541 pKa = 10.79 EE1542 pKa = 4.17 DD1543 pKa = 3.79 FEE1545 pKa = 4.86 DD1546 pKa = 4.16 LEE1548 pKa = 4.47 RR1549 pKa = 11.84 LGEE1552 pKa = 4.18 TFSIISGSPVTRR1564 pKa = 11.84 NRR1566 pKa = 11.84 PRR1568 pKa = 11.84 VTTRR1572 pKa = 11.84 VTITGSNAGSTFSLLDD1588 pKa = 3.0 IVRR1591 pKa = 11.84 FMWFGTSHH1599 pKa = 4.8 VHH1601 pKa = 6.86 ASPGHH1606 pKa = 5.78 LQNLWEE1612 pKa = 4.06 EE1613 pKa = 4.47 SKK1615 pKa = 10.96 RR1616 pKa = 11.84 RR1617 pKa = 11.84 IPWLRR1622 pKa = 11.84 DD1623 pKa = 3.38 TPGEE1627 pKa = 4.05 TLEE1630 pKa = 4.69 ASPFVDD1636 pKa = 3.68 PQSLHH1641 pKa = 6.13 NFIAGDD1647 pKa = 3.68 PVKK1650 pKa = 10.83 GRR1652 pKa = 11.84 VVVMSGVPVKK1662 pKa = 10.66 RR1663 pKa = 11.84 SFGVSNLYY1671 pKa = 10.29 TMVSEE1676 pKa = 4.47 NFAPQYY1682 pKa = 10.85 RR1683 pKa = 11.84 LANSVDD1689 pKa = 3.29 VEE1691 pKa = 4.11 ARR1693 pKa = 11.84 GRR1695 pKa = 11.84 TEE1697 pKa = 4.06 GFIDD1701 pKa = 4.07 LRR1703 pKa = 11.84 HH1704 pKa = 5.25 TLGLISQGFFHH1715 pKa = 7.59 DD1716 pKa = 3.86 AVKK1719 pKa = 10.86 VDD1721 pKa = 3.66 LTKK1724 pKa = 10.62 QVLKK1728 pKa = 10.83 ANVRR1732 pKa = 11.84 IEE1734 pKa = 4.06 VDD1736 pKa = 2.89 TGAARR1741 pKa = 11.84 SRR1743 pKa = 11.84 RR1744 pKa = 11.84 NCLAVIQDD1752 pKa = 3.86 FAVHH1756 pKa = 6.35 EE1757 pKa = 4.93 DD1758 pKa = 3.26 IEE1760 pKa = 4.57 RR1761 pKa = 11.84 TKK1763 pKa = 11.19 TMIFSHH1769 pKa = 6.72 KK1770 pKa = 9.97 LGSLGGYY1777 pKa = 7.06 SVRR1780 pKa = 11.84 QRR1782 pKa = 11.84 LVRR1785 pKa = 11.84 PEE1787 pKa = 3.76 EE1788 pKa = 4.08 VQEE1791 pKa = 4.56 GEE1793 pKa = 3.68 QSGYY1797 pKa = 10.2 FGRR1800 pKa = 11.84 GVWRR1804 pKa = 11.84 GFYY1807 pKa = 10.52 DD1808 pKa = 3.55 STGLEE1813 pKa = 3.71 IHH1815 pKa = 7.25 LDD1817 pKa = 3.67 RR1818 pKa = 11.84 PRR1820 pKa = 11.84 TDD1822 pKa = 3.48 PLSHH1826 pKa = 5.88 ITAVVVSGTHH1836 pKa = 6.72 DD1837 pKa = 4.0 LNNVIRR1843 pKa = 11.84 FLKK1846 pKa = 10.38 EE1847 pKa = 3.05 WGKK1850 pKa = 9.97 EE1851 pKa = 3.78 NKK1853 pKa = 9.56 VNNDD1857 pKa = 2.95 VHH1859 pKa = 7.29 CRR1861 pKa = 11.84 GPHH1864 pKa = 4.25 VPKK1867 pKa = 10.32 GRR1869 pKa = 11.84 NVARR1873 pKa = 11.84 IHH1875 pKa = 6.41 GFSFCLGEE1883 pKa = 4.05 RR1884 pKa = 11.84 GAPVLVNEE1892 pKa = 4.61 RR1893 pKa = 11.84 VAPYY1897 pKa = 10.14 LHH1899 pKa = 6.98 SEE1901 pKa = 4.13 EE1902 pKa = 4.12 FAKK1905 pKa = 11.08 VEE1907 pKa = 4.09 MTVSGGTIRR1916 pKa = 11.84 LVATPRR1922 pKa = 11.84 YY1923 pKa = 8.6 QGARR1927 pKa = 11.84 SGTITLLSYY1936 pKa = 9.29 TSRR1939 pKa = 11.84 ASDD1942 pKa = 3.06 IDD1944 pKa = 3.6 VMRR1947 pKa = 11.84 RR1948 pKa = 11.84 GVATKK1953 pKa = 10.57 LGLKK1957 pKa = 10.13 SRR1959 pKa = 11.84 GLNSYY1964 pKa = 8.59 VRR1966 pKa = 11.84 AWLDD1970 pKa = 3.94 CIPLSSQQFEE1980 pKa = 4.52 SLIQRR1985 pKa = 11.84 LKK1987 pKa = 10.54 RR1988 pKa = 11.84 EE1989 pKa = 4.18 RR1990 pKa = 11.84 GTGAVPKK1997 pKa = 8.8 PHH1999 pKa = 6.39 WEE2001 pKa = 4.09 GRR2003 pKa = 11.84 KK2004 pKa = 9.73 DD2005 pKa = 5.0 GYY2007 pKa = 11.37 VDD2009 pKa = 3.35 WPKK2012 pKa = 10.93 LEE2014 pKa = 3.89 SRR2016 pKa = 11.84 LRR2018 pKa = 11.84 EE2019 pKa = 3.88 LGEE2022 pKa = 3.56 IQLRR2026 pKa = 11.84 YY2027 pKa = 10.29 SGILRR2032 pKa = 11.84 MSHH2035 pKa = 6.31 APEE2038 pKa = 4.21 GQEE2041 pKa = 4.6 TEE2043 pKa = 3.98 NLDD2046 pKa = 3.16 ISEE2049 pKa = 4.78 EE2050 pKa = 3.99 FDD2052 pKa = 3.16 PSYY2055 pKa = 11.3 FDD2057 pKa = 6.42 DD2058 pKa = 5.62 FFTDD2062 pKa = 4.66 DD2063 pKa = 3.26 ATADD2067 pKa = 3.98 LGVMLDD2073 pKa = 3.67 VLQVTTPVEE2082 pKa = 3.68 EE2083 pKa = 5.16 DD2084 pKa = 3.71 GNVMEE2089 pKa = 5.14 EE2090 pKa = 5.98 DD2091 pKa = 3.32 NMFWNVGDD2099 pKa = 4.1 EE2100 pKa = 4.03 EE2101 pKa = 4.87 MEE2103 pKa = 4.16 FGMDD2107 pKa = 5.57 LIRR2110 pKa = 11.84 QLEE2113 pKa = 4.12 FQEE2116 pKa = 3.82 ARR2118 pKa = 11.84 EE2119 pKa = 4.5 EE2120 pKa = 3.79 IRR2122 pKa = 11.84 ITHH2125 pKa = 7.4 PYY2127 pKa = 9.93 LKK2129 pKa = 10.49 VAVEE2133 pKa = 4.14 QIVGALGGTEE2143 pKa = 3.73 RR2144 pKa = 11.84 AIRR2147 pKa = 11.84 AIMTRR2152 pKa = 11.84 SVPASVKK2159 pKa = 10.73 DD2160 pKa = 3.79 SVVDD2164 pKa = 3.68 ALVVIFNWWGQHH2176 pKa = 4.4 VDD2178 pKa = 3.79 RR2179 pKa = 11.84 PLHH2182 pKa = 5.71 SAGSDD2187 pKa = 3.39 DD2188 pKa = 4.23 EE2189 pKa = 5.71 EE2190 pKa = 4.83 EE2191 pKa = 4.43 MGDD2194 pKa = 5.39 DD2195 pKa = 5.06 VDD2197 pKa = 5.8 DD2198 pKa = 4.16 LL2199 pKa = 4.56
Molecular weight: 249.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.086
IPC2_protein 5.995
IPC_protein 6.071
Toseland 6.465
ProMoST 6.453
Dawson 6.364
Bjellqvist 6.376
Wikipedia 6.389
Rodwell 6.364
Grimsley 6.62
Solomon 6.364
Lehninger 6.364
Nozaki 6.649
DTASelect 6.825
Thurlkill 6.854
EMBOSS 6.81
Sillero 6.751
Patrickios 4.469
IPC_peptide 6.389
IPC2_peptide 6.664
IPC2.peptide.svr19 6.694
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
2601
402
2199
1300.5
146.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.305 ± 1.507
1.269 ± 0.011
5.075 ± 0.486
8.189 ± 0.009
4.191 ± 0.866
6.151 ± 0.03
2.653 ± 0.736
4.306 ± 0.187
6.113 ± 0.395
9.65 ± 0.308
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.306 ± 0.299
3.191 ± 0.019
4.537 ± 0.747
3.729 ± 0.222
6.19 ± 0.098
7.805 ± 1.035
5.613 ± 0.6
7.689 ± 0.562
1.499 ± 0.334
2.537 ± 0.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here