Striped jack nervous necrosis virus (SjNNV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Magsaviricetes; Nodamuvirales; Nodaviridae; Betanodavirus

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9QAZ8|RDRP_SJNNV RNA-directed RNA polymerase OS=Striped jack nervous necrosis virus OX=35297 PE=3 SV=1
MM1 pKa = 7.29EE2 pKa = 4.59QVQQAIDD9 pKa = 3.29QHH11 pKa = 7.44LVEE14 pKa = 5.16LEE16 pKa = 4.05QLFKK20 pKa = 10.76MLMDD24 pKa = 3.29VRR26 pKa = 11.84VALGGVTVVQVNEE39 pKa = 3.64MRR41 pKa = 11.84TFVISASAAAQRR53 pKa = 11.84LRR55 pKa = 11.84ALARR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 7.53PAPLAVAAEE70 pKa = 4.09PMEE73 pKa = 4.49TEE75 pKa = 3.92

Molecular weight: 8.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9QAZ7|B_SJNNV Protein B OS=Striped jack nervous necrosis virus OX=35297 PE=4 SV=1
MM1 pKa = 7.0VRR3 pKa = 11.84KK4 pKa = 9.73GDD6 pKa = 3.65KK7 pKa = 10.35KK8 pKa = 10.41LAKK11 pKa = 10.21PPTTKK16 pKa = 10.51AANSQPRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84ATQRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84SGRR34 pKa = 11.84ADD36 pKa = 3.27APLAKK41 pKa = 10.21ASTITGFGRR50 pKa = 11.84ATNDD54 pKa = 2.97VHH56 pKa = 8.04ISGMSRR62 pKa = 11.84IAQAVVPAGTGTDD75 pKa = 3.25GKK77 pKa = 10.48IVVDD81 pKa = 3.61STIVPEE87 pKa = 3.99LLPRR91 pKa = 11.84LGHH94 pKa = 6.87AARR97 pKa = 11.84IFQRR101 pKa = 11.84YY102 pKa = 8.09AVEE105 pKa = 3.9TLEE108 pKa = 5.81FEE110 pKa = 4.34IQPMCPANTGGGYY123 pKa = 9.72VAGFLPDD130 pKa = 3.64PTDD133 pKa = 3.5NDD135 pKa = 3.63HH136 pKa = 7.11TFDD139 pKa = 4.08ALQATRR145 pKa = 11.84GAVVAKK151 pKa = 8.41WWEE154 pKa = 4.1SRR156 pKa = 11.84TVRR159 pKa = 11.84PQYY162 pKa = 10.64TRR164 pKa = 11.84TLLWTSTGKK173 pKa = 8.32EE174 pKa = 3.75QRR176 pKa = 11.84LTSPGRR182 pKa = 11.84LVLLCVGSNTDD193 pKa = 3.35VVNVSVMCRR202 pKa = 11.84WSVRR206 pKa = 11.84LSVPSLEE213 pKa = 4.4TPEE216 pKa = 4.81DD217 pKa = 3.34TTAPITTQAPLHH229 pKa = 6.35NDD231 pKa = 3.79SINNGYY237 pKa = 8.18TGFRR241 pKa = 11.84SILLGATQLDD251 pKa = 4.44LAPANAVFVTDD262 pKa = 3.98KK263 pKa = 10.63PLPIDD268 pKa = 3.6YY269 pKa = 10.36NLGVGDD275 pKa = 3.89VDD277 pKa = 4.33RR278 pKa = 11.84AVYY281 pKa = 8.48WHH283 pKa = 6.63LRR285 pKa = 11.84KK286 pKa = 9.72KK287 pKa = 10.78AGDD290 pKa = 3.66TQVPAGYY297 pKa = 9.9FDD299 pKa = 3.22WGLWDD304 pKa = 4.95DD305 pKa = 4.07FNKK308 pKa = 9.77TFTVGAPYY316 pKa = 10.82YY317 pKa = 10.5SDD319 pKa = 3.28QQPRR323 pKa = 11.84QILLPAGTLFTRR335 pKa = 11.84VDD337 pKa = 3.75SEE339 pKa = 4.19NN340 pKa = 3.16

Molecular weight: 37.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1398

75

983

466.0

52.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.013 ± 1.017

1.216 ± 0.251

6.652 ± 0.524

4.578 ± 0.832

3.076 ± 0.07

6.438 ± 0.652

2.289 ± 0.406

3.934 ± 0.28

3.934 ± 0.371

8.155 ± 0.353

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.717 ± 0.626

3.505 ± 0.276

5.651 ± 0.548

4.649 ± 0.592

8.369 ± 0.322

6.652 ± 0.911

7.368 ± 1.027

7.582 ± 0.912

1.431 ± 0.264

3.791 ± 0.672
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski