Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides thetaiotaomicron

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4782 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q89Z02|Q89Z02_BACTN Uncharacterized protein OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) OX=226186 GN=BT_4575 PE=4 SV=1
MM1 pKa = 7.58LKK3 pKa = 10.47FKK5 pKa = 10.66FGAWAVVLMLTAFSFSACDD24 pKa = 3.9DD25 pKa = 4.25NDD27 pKa = 4.11DD28 pKa = 3.73EE29 pKa = 5.27TYY31 pKa = 11.07NPPANITEE39 pKa = 4.32ALKK42 pKa = 10.5QLYY45 pKa = 9.8PNAQNVEE52 pKa = 3.91WEE54 pKa = 4.36MKK56 pKa = 9.77GDD58 pKa = 4.08YY59 pKa = 10.28YY60 pKa = 11.67VADD63 pKa = 3.66CWVTGDD69 pKa = 4.82EE70 pKa = 4.31LDD72 pKa = 3.44VWFDD76 pKa = 4.11ANANWVMTEE85 pKa = 4.13NEE87 pKa = 4.74LDD89 pKa = 4.42SIDD92 pKa = 3.83QLVPAVYY99 pKa = 9.68TGFRR103 pKa = 11.84NSNYY107 pKa = 9.88SSWVVTDD114 pKa = 3.72VFVLTYY120 pKa = 8.74PQHH123 pKa = 5.61PTEE126 pKa = 4.56SVIQVKK132 pKa = 10.11QGNLRR137 pKa = 11.84FALYY141 pKa = 10.07FSQEE145 pKa = 3.42GGLLHH150 pKa = 6.93EE151 pKa = 5.02RR152 pKa = 11.84DD153 pKa = 3.54ITNGDD158 pKa = 3.49DD159 pKa = 3.86TNWPVMEE166 pKa = 4.47

Molecular weight: 19.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q8A5S5|RS6_BACTN 30S ribosomal protein S6 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) OX=226186 GN=rpsF PE=3 SV=1
MM1 pKa = 7.62ARR3 pKa = 11.84NCRR6 pKa = 11.84NSSVSLTTYY15 pKa = 10.41SIVARR20 pKa = 11.84SVRR23 pKa = 11.84ITLNSSLKK31 pKa = 10.01IRR33 pKa = 11.84AAMNLDD39 pKa = 3.52LDD41 pKa = 4.15NRR43 pKa = 11.84PDD45 pKa = 3.61CSNMVRR51 pKa = 11.84NRR53 pKa = 11.84AWPASSRR60 pKa = 11.84LTVSRR65 pKa = 11.84RR66 pKa = 11.84MAGFGLGNLPPFPRR80 pKa = 11.84VRR82 pKa = 11.84GRR84 pKa = 11.84FFSLFIYY91 pKa = 9.07TFFMWYY97 pKa = 10.6NSTGWNCLRR106 pKa = 11.84QLPLPQFATSEE117 pKa = 4.1QIAAPLQGQAFFAAKK132 pKa = 10.16RR133 pKa = 11.84SGAAKK138 pKa = 8.85NTACYY143 pKa = 10.57VLANINLSSLEE154 pKa = 3.83RR155 pKa = 11.84KK156 pKa = 9.07EE157 pKa = 3.81RR158 pKa = 11.84FRR160 pKa = 11.84GKK162 pKa = 10.25RR163 pKa = 11.84GLVTDD168 pKa = 4.74GRR170 pKa = 11.84QTAHH174 pKa = 6.37SRR176 pKa = 11.84LDD178 pKa = 3.6GAKK181 pKa = 9.82LGSDD185 pKa = 4.01IISCISLTLATLCQMEE201 pKa = 4.62ATCAHH206 pKa = 6.11GWNRR210 pKa = 11.84RR211 pKa = 11.84KK212 pKa = 10.3NGRR215 pKa = 11.84PMMAEE220 pKa = 3.91SAVEE224 pKa = 5.52DD225 pKa = 3.95VPEE228 pKa = 3.93WDD230 pKa = 3.3AA231 pKa = 5.11

Molecular weight: 25.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4782

0

4782

1864427

32

2183

389.9

44.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.842 ± 0.032

1.22 ± 0.013

5.54 ± 0.023

6.516 ± 0.031

4.57 ± 0.021

6.78 ± 0.032

1.815 ± 0.014

6.889 ± 0.028

6.68 ± 0.025

9.016 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.717 ± 0.013

5.259 ± 0.029

3.784 ± 0.019

3.504 ± 0.018

4.546 ± 0.023

6.297 ± 0.026

5.709 ± 0.026

6.295 ± 0.026

1.365 ± 0.014

4.656 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski