Eubacterium sp. CAG:251

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1717 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6QJ07|R6QJ07_9FIRM UDP-N-acetylglucosamine 1-carboxyvinyltransferase OS=Eubacterium sp. CAG:251 OX=1262886 GN=murA PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 10.34KK3 pKa = 10.12IITIFLGVCISIFFVSCSTPYY24 pKa = 10.78YY25 pKa = 9.42NQNSDD30 pKa = 4.5LPNANNTKK38 pKa = 10.73DD39 pKa = 3.89FIHH42 pKa = 6.93YY43 pKa = 9.33EE44 pKa = 4.13VEE46 pKa = 3.65FDD48 pKa = 4.31IPNEE52 pKa = 3.84YY53 pKa = 8.65TAYY56 pKa = 9.5STDD59 pKa = 3.2NEE61 pKa = 5.1FYY63 pKa = 10.64TEE65 pKa = 5.83LIINDD70 pKa = 4.02NIDD73 pKa = 3.03VRR75 pKa = 11.84KK76 pKa = 9.82FKK78 pKa = 10.99EE79 pKa = 4.09EE80 pKa = 4.06NDD82 pKa = 3.44NTDD85 pKa = 3.14CDD87 pKa = 4.23VYY89 pKa = 10.5FTDD92 pKa = 6.37EE93 pKa = 4.92IIDD96 pKa = 3.83SNAEE100 pKa = 3.88VIYY103 pKa = 9.95TLVFEE108 pKa = 4.56FNCYY112 pKa = 10.32DD113 pKa = 3.77EE114 pKa = 4.85NDD116 pKa = 3.65NLIDD120 pKa = 3.92TIYY123 pKa = 9.46FTDD126 pKa = 3.55TLGDD130 pKa = 3.43KK131 pKa = 10.76QGIGGEE137 pKa = 4.3NVLSSTVNEE146 pKa = 4.79SVRR149 pKa = 11.84KK150 pKa = 8.93MKK152 pKa = 10.61FSKK155 pKa = 10.9AFLADD160 pKa = 3.69LNEE163 pKa = 4.09VQFYY167 pKa = 10.46NN168 pKa = 4.14

Molecular weight: 19.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6QRZ9|R6QRZ9_9FIRM Protein translocase subunit SecA OS=Eubacterium sp. CAG:251 OX=1262886 GN=secA PE=3 SV=1
MM1 pKa = 7.88AYY3 pKa = 10.15NRR5 pKa = 11.84AGGPRR10 pKa = 11.84KK11 pKa = 9.57GRR13 pKa = 11.84KK14 pKa = 8.43KK15 pKa = 9.76VCVFCVEE22 pKa = 4.21KK23 pKa = 10.85VDD25 pKa = 4.4EE26 pKa = 4.47IDD28 pKa = 3.79YY29 pKa = 11.26KK30 pKa = 11.3DD31 pKa = 3.38VTRR34 pKa = 11.84LKK36 pKa = 10.53RR37 pKa = 11.84FVSEE41 pKa = 3.99RR42 pKa = 11.84SKK44 pKa = 10.61ILPRR48 pKa = 11.84RR49 pKa = 11.84VTGTCAKK56 pKa = 9.65HH57 pKa = 5.38QRR59 pKa = 11.84EE60 pKa = 4.47LTTAIKK66 pKa = 10.25RR67 pKa = 11.84ARR69 pKa = 11.84HH70 pKa = 5.41LALLPYY76 pKa = 10.25TSDD79 pKa = 3.02

Molecular weight: 9.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1717

0

1717

555267

29

3485

323.4

36.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.179 ± 0.06

1.68 ± 0.025

6.222 ± 0.048

6.492 ± 0.065

4.488 ± 0.044

6.619 ± 0.057

1.452 ± 0.024

8.06 ± 0.054

8.562 ± 0.061

8.209 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.61 ± 0.029

5.761 ± 0.065

3.014 ± 0.033

2.532 ± 0.028

3.324 ± 0.045

6.469 ± 0.053

5.507 ± 0.052

6.846 ± 0.053

0.749 ± 0.021

4.224 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski