candidate division MSBL1 archaeon SCGC-AAA259I14

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 774 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133UU88|A0A133UU88_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259I14 OX=1698268 GN=AKJ38_00535 PE=4 SV=1
MM1 pKa = 7.51TEE3 pKa = 4.19VICAVPGCEE12 pKa = 4.75NNLTDD17 pKa = 3.76EE18 pKa = 4.07QRR20 pKa = 11.84EE21 pKa = 4.12NKK23 pKa = 9.94IPVCSEE29 pKa = 3.67CEE31 pKa = 3.71AANMHH36 pKa = 5.76VCEE39 pKa = 4.51SCNKK43 pKa = 9.86RR44 pKa = 11.84LSTEE48 pKa = 3.91QIQNGGSLCSEE59 pKa = 4.61CEE61 pKa = 4.15GNPSGLEE68 pKa = 3.81EE69 pKa = 4.35SMEE72 pKa = 4.4EE73 pKa = 4.01YY74 pKa = 10.79GEE76 pKa = 4.15EE77 pKa = 4.37YY78 pKa = 10.64IMGTDD83 pKa = 3.43TEE85 pKa = 4.67EE86 pKa = 3.79EE87 pKa = 4.45GFMVGEE93 pKa = 4.32SKK95 pKa = 10.67EE96 pKa = 5.11DD97 pKa = 3.44EE98 pKa = 4.35DD99 pKa = 5.95FMVV102 pKa = 3.97

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133URH1|A0A133URH1_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA259I14 OX=1698268 GN=AKJ38_02515 PE=4 SV=1
MM1 pKa = 7.84LDD3 pKa = 4.52LIRR6 pKa = 11.84LDD8 pKa = 3.49RR9 pKa = 11.84CSSKK13 pKa = 10.62LSKK16 pKa = 10.57LSYY19 pKa = 10.48FSTSLSIPRR28 pKa = 11.84TSPLSLTPINRR39 pKa = 11.84VPPSVFKK46 pKa = 11.06NAVIVFNTSFLRR58 pKa = 11.84LFSVYY63 pKa = 10.16ISLL66 pKa = 4.79

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

774

0

774

179292

38

1181

231.6

26.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.873 ± 0.096

1.075 ± 0.042

5.824 ± 0.075

10.258 ± 0.134

3.922 ± 0.076

7.193 ± 0.088

1.64 ± 0.037

7.185 ± 0.075

7.99 ± 0.112

9.158 ± 0.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.179 ± 0.033

3.849 ± 0.061

4.04 ± 0.054

2.253 ± 0.054

5.746 ± 0.092

6.231 ± 0.069

4.649 ± 0.055

6.748 ± 0.072

1.163 ± 0.041

3.024 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski