Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Thermotogales; Fervidobacteriaceae; Fervidobacterium; Fervidobacterium nodosum

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1725 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7HNJ2|A7HNJ2_FERNB Transglutaminase domain protein OS=Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) OX=381764 GN=Fnod_1640 PE=4 SV=1
MM1 pKa = 7.25EE2 pKa = 4.96RR3 pKa = 11.84MEE5 pKa = 5.54RR6 pKa = 11.84ILFGKK11 pKa = 10.25FIGDD15 pKa = 3.36VGMMTILVKK24 pKa = 10.81AEE26 pKa = 4.07EE27 pKa = 4.79LDD29 pKa = 4.58NLPNWIWIDD38 pKa = 4.21CEE40 pKa = 4.08EE41 pKa = 4.81DD42 pKa = 3.58SQKK45 pKa = 10.7PDD47 pKa = 3.28YY48 pKa = 11.22VLFEE52 pKa = 4.54ILDD55 pKa = 3.84SVPEE59 pKa = 3.94VGEE62 pKa = 3.73IDD64 pKa = 3.5GNIYY68 pKa = 10.04YY69 pKa = 9.94IYY71 pKa = 11.18GEE73 pKa = 4.15FNEE76 pKa = 4.54EE77 pKa = 4.37KK78 pKa = 9.52YY79 pKa = 9.71QHH81 pKa = 6.92AEE83 pKa = 4.22PITTFQDD90 pKa = 3.43YY91 pKa = 11.09SIDD94 pKa = 3.7LDD96 pKa = 4.07FFQTIQDD103 pKa = 3.46EE104 pKa = 4.85CEE106 pKa = 3.93WEE108 pKa = 4.0IVHH111 pKa = 6.18YY112 pKa = 10.75LDD114 pKa = 4.34YY115 pKa = 11.52

Molecular weight: 13.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A7HLW6|A7HLW6_FERNB Uncharacterized protein OS=Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1) OX=381764 GN=Fnod_1050 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.38RR3 pKa = 11.84TYY5 pKa = 10.32QPSQRR10 pKa = 11.84KK11 pKa = 8.79RR12 pKa = 11.84KK13 pKa = 7.9RR14 pKa = 11.84THH16 pKa = 6.08GFLARR21 pKa = 11.84KK22 pKa = 6.68STPGGRR28 pKa = 11.84KK29 pKa = 7.51VLRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84TGRR39 pKa = 11.84WRR41 pKa = 11.84LTVV44 pKa = 3.17

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1725

0

1725

576878

38

1650

334.4

37.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.598 ± 0.057

0.635 ± 0.018

5.032 ± 0.043

7.848 ± 0.068

5.131 ± 0.047

6.397 ± 0.054

1.418 ± 0.026

9.226 ± 0.05

8.584 ± 0.061

9.383 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.025

5.089 ± 0.048

3.442 ± 0.034

2.459 ± 0.027

3.907 ± 0.038

6.026 ± 0.047

4.878 ± 0.039

7.432 ± 0.044

0.9 ± 0.024

4.328 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski