Enterococcus cecorum DSM 20682 = ATCC 43198

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus cecorum

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2361 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|S1QX95|S1QX95_9ENTE 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Enterococcus cecorum DSM 20682 = ATCC 43198 OX=1121864 GN=fabZ PE=3 SV=1
MM1 pKa = 7.05IVAYY5 pKa = 7.31EE6 pKa = 4.12TFAFVYY12 pKa = 10.37DD13 pKa = 3.92EE14 pKa = 4.82VMDD17 pKa = 3.83TSLYY21 pKa = 10.05QRR23 pKa = 11.84WLDD26 pKa = 3.43FSLRR30 pKa = 11.84FLGDD34 pKa = 2.98RR35 pKa = 11.84KK36 pKa = 10.46RR37 pKa = 11.84ILEE40 pKa = 4.24LACGTGALALDD51 pKa = 4.53FAKK54 pKa = 10.74SGFDD58 pKa = 3.31VTALDD63 pKa = 4.28LSEE66 pKa = 4.4EE67 pKa = 4.16MLMIASSRR75 pKa = 11.84AQEE78 pKa = 3.98ADD80 pKa = 2.85VDD82 pKa = 3.92IQFVEE87 pKa = 4.21GDD89 pKa = 3.91MLDD92 pKa = 3.48LTDD95 pKa = 3.72IDD97 pKa = 3.68TYY99 pKa = 11.29EE100 pKa = 4.18AVTCFSDD107 pKa = 3.93SICYY111 pKa = 9.44MEE113 pKa = 6.02DD114 pKa = 3.2EE115 pKa = 4.39QAVQQVFDD123 pKa = 3.7GVYY126 pKa = 10.3QILEE130 pKa = 4.16EE131 pKa = 4.18NGVFIFDD138 pKa = 3.44VHH140 pKa = 5.31STYY143 pKa = 10.77QIDD146 pKa = 4.25EE147 pKa = 4.25VFPDD151 pKa = 3.5YY152 pKa = 10.85SYY154 pKa = 11.22HH155 pKa = 5.76YY156 pKa = 8.56QTEE159 pKa = 4.21DD160 pKa = 4.2FAFLWDD166 pKa = 3.98SYY168 pKa = 11.74AVDD171 pKa = 3.78VPHH174 pKa = 7.51SIEE177 pKa = 4.04HH178 pKa = 6.42FLTFFVQNEE187 pKa = 4.03DD188 pKa = 3.52GLFEE192 pKa = 4.48RR193 pKa = 11.84RR194 pKa = 11.84DD195 pKa = 3.57EE196 pKa = 4.03IHH198 pKa = 7.27RR199 pKa = 11.84EE200 pKa = 3.33RR201 pKa = 11.84TYY203 pKa = 11.14PLNQYY208 pKa = 11.5LMMLEE213 pKa = 3.89NAGFAKK219 pKa = 10.55VEE221 pKa = 4.22AYY223 pKa = 10.67ADD225 pKa = 4.64FIDD228 pKa = 4.68SEE230 pKa = 4.61PNEE233 pKa = 4.01KK234 pKa = 9.92SRR236 pKa = 11.84RR237 pKa = 11.84WFFVCEE243 pKa = 3.94KK244 pKa = 10.79

Molecular weight:
28.59 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S1RPZ6|S1RPZ6_9ENTE Uncharacterized protein OS=Enterococcus cecorum DSM 20682 = ATCC 43198 OX=1121864 GN=OMO_01749 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.76VLAAA44 pKa = 4.31

Molecular weight:
5.34 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2361

0

2361

690344

20

1631

292.4

33.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.18 ± 0.059

0.781 ± 0.016

5.229 ± 0.039

7.023 ± 0.05

4.63 ± 0.04

6.137 ± 0.049

2.048 ± 0.022

7.543 ± 0.051

7.182 ± 0.044

10.013 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.711 ± 0.025

4.739 ± 0.038

3.34 ± 0.023

4.959 ± 0.053

3.829 ± 0.039

5.666 ± 0.038

5.448 ± 0.037

6.711 ± 0.04

0.861 ± 0.018

3.972 ± 0.034

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski