Enterococcus cecorum DSM 20682 = ATCC 43198
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2361 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S1QX95|S1QX95_9ENTE 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Enterococcus cecorum DSM 20682 = ATCC 43198 OX=1121864 GN=fabZ PE=3 SV=1
MM1 pKa = 7.05 IVAYY5 pKa = 7.31 EE6 pKa = 4.12 TFAFVYY12 pKa = 10.37 DD13 pKa = 3.92 EE14 pKa = 4.82 VMDD17 pKa = 3.83 TSLYY21 pKa = 10.05 QRR23 pKa = 11.84 WLDD26 pKa = 3.43 FSLRR30 pKa = 11.84 FLGDD34 pKa = 2.98 RR35 pKa = 11.84 KK36 pKa = 10.46 RR37 pKa = 11.84 ILEE40 pKa = 4.24 LACGTGALALDD51 pKa = 4.53 FAKK54 pKa = 10.74 SGFDD58 pKa = 3.31 VTALDD63 pKa = 4.28 LSEE66 pKa = 4.4 EE67 pKa = 4.16 MLMIASSRR75 pKa = 11.84 AQEE78 pKa = 3.98 ADD80 pKa = 2.85 VDD82 pKa = 3.92 IQFVEE87 pKa = 4.21 GDD89 pKa = 3.91 MLDD92 pKa = 3.48 LTDD95 pKa = 3.72 IDD97 pKa = 3.68 TYY99 pKa = 11.29 EE100 pKa = 4.18 AVTCFSDD107 pKa = 3.93 SICYY111 pKa = 9.44 MEE113 pKa = 6.02 DD114 pKa = 3.2 EE115 pKa = 4.39 QAVQQVFDD123 pKa = 3.7 GVYY126 pKa = 10.3 QILEE130 pKa = 4.16 EE131 pKa = 4.18 NGVFIFDD138 pKa = 3.44 VHH140 pKa = 5.31 STYY143 pKa = 10.77 QIDD146 pKa = 4.25 EE147 pKa = 4.25 VFPDD151 pKa = 3.5 YY152 pKa = 10.85 SYY154 pKa = 11.22 HH155 pKa = 5.76 YY156 pKa = 8.56 QTEE159 pKa = 4.21 DD160 pKa = 4.2 FAFLWDD166 pKa = 3.98 SYY168 pKa = 11.74 AVDD171 pKa = 3.78 VPHH174 pKa = 7.51 SIEE177 pKa = 4.04 HH178 pKa = 6.42 FLTFFVQNEE187 pKa = 4.03 DD188 pKa = 3.52 GLFEE192 pKa = 4.48 RR193 pKa = 11.84 RR194 pKa = 11.84 DD195 pKa = 3.57 EE196 pKa = 4.03 IHH198 pKa = 7.27 RR199 pKa = 11.84 EE200 pKa = 3.33 RR201 pKa = 11.84 TYY203 pKa = 11.14 PLNQYY208 pKa = 11.5 LMMLEE213 pKa = 3.89 NAGFAKK219 pKa = 10.55 VEE221 pKa = 4.22 AYY223 pKa = 10.67 ADD225 pKa = 4.64 FIDD228 pKa = 4.68 SEE230 pKa = 4.61 PNEE233 pKa = 4.01 KK234 pKa = 9.92 SRR236 pKa = 11.84 RR237 pKa = 11.84 WFFVCEE243 pKa = 3.94 KK244 pKa = 10.79
Molecular weight: 28.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.986
IPC_protein 3.973
Toseland 3.77
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.859
Rodwell 3.808
Grimsley 3.681
Solomon 3.948
Lehninger 3.897
Nozaki 4.062
DTASelect 4.266
Thurlkill 3.808
EMBOSS 3.872
Sillero 4.088
Patrickios 1.316
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 3.98
Protein with the highest isoelectric point:
>tr|S1RPZ6|S1RPZ6_9ENTE Uncharacterized protein OS=Enterococcus cecorum DSM 20682 = ATCC 43198 OX=1121864 GN=OMO_01749 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 9.16 RR10 pKa = 11.84 KK11 pKa = 9.12 RR12 pKa = 11.84 QKK14 pKa = 9.38 VHH16 pKa = 5.8 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 9.46 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.8 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLAAA44 pKa = 4.31
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2361
0
2361
690344
20
1631
292.4
33.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.18 ± 0.059
0.781 ± 0.016
5.229 ± 0.039
7.023 ± 0.05
4.63 ± 0.04
6.137 ± 0.049
2.048 ± 0.022
7.543 ± 0.051
7.182 ± 0.044
10.013 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.711 ± 0.025
4.739 ± 0.038
3.34 ± 0.023
4.959 ± 0.053
3.829 ± 0.039
5.666 ± 0.038
5.448 ± 0.037
6.711 ± 0.04
0.861 ± 0.018
3.972 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here