Actinoplanes regularis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group;

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8959 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239F9Z2|A0A239F9Z2_9ACTN Hydrogenase maturation factor HypA OS=Actinoplanes regularis OX=52697 GN=hypA PE=3 SV=1
MM1 pKa = 8.19DD2 pKa = 6.73DD3 pKa = 5.58DD4 pKa = 5.43DD5 pKa = 4.61LHH7 pKa = 6.59PLAVLGWRR15 pKa = 11.84DD16 pKa = 3.19PTGYY20 pKa = 10.22LVEE23 pKa = 4.54PGKK26 pKa = 10.04PEE28 pKa = 3.68EE29 pKa = 4.71SITNGFLNPLDD40 pKa = 4.22LFNAVSPTAWLNSAIEE56 pKa = 4.16ALTGVDD62 pKa = 3.8FLGWCTEE69 pKa = 3.87WLSGDD74 pKa = 3.32WEE76 pKa = 4.82GVWRR80 pKa = 11.84FGDD83 pKa = 3.42AMGNLAEE90 pKa = 4.78CMQQIGVDD98 pKa = 3.98LQAGFSQVDD107 pKa = 3.98GTWDD111 pKa = 3.51GNASDD116 pKa = 3.39SAYY119 pKa = 10.51AYY121 pKa = 9.96FSNLATRR128 pKa = 11.84TSGQQVAIFPTEE140 pKa = 3.93SAYY143 pKa = 10.72HH144 pKa = 6.07KK145 pKa = 10.03AATGAWQVSNQLGNIAQALADD166 pKa = 3.85NAILAGLVVGASGALMGTGVGAVAGGAGLGAVGLIILDD204 pKa = 3.6MLKK207 pKa = 10.68LINNASLIINTAGTAILGIIGEE229 pKa = 4.46AMSLAYY235 pKa = 10.18QGGDD239 pKa = 3.39LTSLPLPNVPYY250 pKa = 10.41VSPEE254 pKa = 3.38II255 pKa = 4.23

Molecular weight: 26.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A238W4L8|A0A238W4L8_9ACTN Lysophospholipase L1 OS=Actinoplanes regularis OX=52697 GN=SAMN06264365_102165 PE=4 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8959

0

8959

2964689

24

4264

330.9

35.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.691 ± 0.037

0.746 ± 0.007

5.955 ± 0.018

5.158 ± 0.026

2.808 ± 0.013

9.189 ± 0.026

2.104 ± 0.012

3.683 ± 0.018

1.924 ± 0.019

10.464 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.698 ± 0.01

2.025 ± 0.017

6.043 ± 0.027

2.804 ± 0.017

7.97 ± 0.031

5.136 ± 0.023

6.301 ± 0.029

8.565 ± 0.02

1.61 ± 0.011

2.127 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski