Drosophila A virus
Average proteome isoelectric point is 7.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C6FGJ6|C6FGJ6_9VIRU Capsid protein OS=Drosophila A virus OX=595895 PE=3 SV=1
MM1 pKa = 7.89 DD2 pKa = 5.63 ASNPIISSDD11 pKa = 3.13 RR12 pKa = 11.84 STVNQVYY19 pKa = 10.8 AKK21 pKa = 8.34 MVRR24 pKa = 11.84 RR25 pKa = 11.84 KK26 pKa = 9.91 RR27 pKa = 11.84 QDD29 pKa = 3.14 LAYY32 pKa = 9.99 LQEE35 pKa = 4.49 LVRR38 pKa = 11.84 TAHH41 pKa = 7.52 RR42 pKa = 11.84 EE43 pKa = 3.66 ILPQGEE49 pKa = 4.6 LPHH52 pKa = 6.55 EE53 pKa = 4.74 SEE55 pKa = 4.14 AKK57 pKa = 10.34 QYY59 pKa = 9.04 VAEE62 pKa = 4.26 YY63 pKa = 9.9 KK64 pKa = 10.69 ARR66 pKa = 11.84 LPVVDD71 pKa = 3.8 TKK73 pKa = 11.25 KK74 pKa = 10.75 VGEE77 pKa = 4.42 LPTEE81 pKa = 4.42 VFQVALPSIPVRR93 pKa = 11.84 GIMDD97 pKa = 4.02 AVGVPLHH104 pKa = 6.6 PPSINNRR111 pKa = 11.84 GGHH114 pKa = 4.3 THH116 pKa = 5.49 VTRR119 pKa = 11.84 QMGGVPHH126 pKa = 7.35 PPLGLIPIAQKK137 pKa = 10.91 YY138 pKa = 8.65 GVEE141 pKa = 4.08 ILDD144 pKa = 3.53 QKK146 pKa = 10.86 HH147 pKa = 6.03 VYY149 pKa = 8.85 TGGTWDD155 pKa = 3.84 GFLSRR160 pKa = 11.84 LAQQMGRR167 pKa = 11.84 TTVPLARR174 pKa = 11.84 TVSAGKK180 pKa = 10.43 KK181 pKa = 8.46 NASDD185 pKa = 3.42 RR186 pKa = 11.84 AIIKK190 pKa = 9.68 LLNKK194 pKa = 9.81 YY195 pKa = 7.53 MPRR198 pKa = 11.84 TGNKK202 pKa = 8.54 SWPGVHH208 pKa = 5.45 TTPEE212 pKa = 3.9 EE213 pKa = 4.79 GILDD217 pKa = 4.91 GIKK220 pKa = 8.85 ITAKK224 pKa = 10.21 SSAGAPYY231 pKa = 8.86 WRR233 pKa = 11.84 HH234 pKa = 5.72 KK235 pKa = 10.91 GEE237 pKa = 4.49 CLDD240 pKa = 4.83 HH241 pKa = 8.26 IIDD244 pKa = 3.7 TGLPVVLKK252 pKa = 10.22 HH253 pKa = 6.23 IKK255 pKa = 10.24 EE256 pKa = 4.25 GTLNQLWRR264 pKa = 11.84 EE265 pKa = 4.0 NPEE268 pKa = 3.83 MFLVEE273 pKa = 4.51 VKK275 pKa = 10.91 NKK277 pKa = 9.66 LDD279 pKa = 3.43 RR280 pKa = 11.84 YY281 pKa = 9.18 EE282 pKa = 3.95 VSKK285 pKa = 11.07 LKK287 pKa = 10.71 EE288 pKa = 3.48 KK289 pKa = 9.57 TRR291 pKa = 11.84 PYY293 pKa = 10.4 VCVPAHH299 pKa = 5.87 WALLFSCLTQGFQEE313 pKa = 4.45 GLQVFSNLDD322 pKa = 3.42 PSTEE326 pKa = 3.95 CSNAYY331 pKa = 9.84 GFSSIAGGLTRR342 pKa = 11.84 MVDD345 pKa = 2.61 WMYY348 pKa = 11.39 ACPKK352 pKa = 9.33 RR353 pKa = 11.84 RR354 pKa = 11.84 GRR356 pKa = 11.84 VVCYY360 pKa = 10.63 GDD362 pKa = 3.82 DD363 pKa = 3.4 ACITFWSQGVLYY375 pKa = 10.45 RR376 pKa = 11.84 VDD378 pKa = 3.88 PDD380 pKa = 4.19 FKK382 pKa = 11.51 QMDD385 pKa = 3.82 GSIDD389 pKa = 3.76 RR390 pKa = 11.84 EE391 pKa = 4.05 DD392 pKa = 3.42 ARR394 pKa = 11.84 ITIEE398 pKa = 3.99 WVLHH402 pKa = 5.56 HH403 pKa = 6.5 LRR405 pKa = 11.84 KK406 pKa = 10.16 DD407 pKa = 3.37 LGVEE411 pKa = 3.84 EE412 pKa = 5.43 TPAFWKK418 pKa = 9.32 TVAAVWLDD426 pKa = 3.59 MAIDD430 pKa = 3.33 PHH432 pKa = 7.58 FIVDD436 pKa = 4.2 GKK438 pKa = 8.74 TVYY441 pKa = 10.03 KK442 pKa = 10.36 KK443 pKa = 10.64 KK444 pKa = 10.35 NPHH447 pKa = 5.6 GLMTGVPGTTLFDD460 pKa = 3.7 TVKK463 pKa = 10.86 SVIVWNEE470 pKa = 3.52 MLDD473 pKa = 3.65 QASVGSIDD481 pKa = 4.79 LLNEE485 pKa = 3.99 AQVVEE490 pKa = 4.08 WMKK493 pKa = 10.86 RR494 pKa = 11.84 QGLVVKK500 pKa = 9.81 EE501 pKa = 4.52 GTWSPVALPARR512 pKa = 11.84 DD513 pKa = 3.81 TEE515 pKa = 4.8 GLITDD520 pKa = 4.16 HH521 pKa = 6.99 KK522 pKa = 10.45 FLGVQIMGVYY532 pKa = 9.12 HH533 pKa = 6.02 RR534 pKa = 11.84 HH535 pKa = 5.26 RR536 pKa = 11.84 VIHH539 pKa = 6.02 VPTMPEE545 pKa = 3.56 SDD547 pKa = 4.04 ALEE550 pKa = 4.0 MMLCQKK556 pKa = 10.76 DD557 pKa = 3.78 NPFEE561 pKa = 4.23 KK562 pKa = 10.44 AVSRR566 pKa = 11.84 TARR569 pKa = 11.84 QRR571 pKa = 11.84 TLYY574 pKa = 11.04 DD575 pKa = 3.18 RR576 pKa = 11.84 MRR578 pKa = 11.84 GLMITMGFSIPRR590 pKa = 11.84 IEE592 pKa = 4.24 EE593 pKa = 3.99 TIHH596 pKa = 6.74 AVVNTIPGEE605 pKa = 4.12 IIVMQTQEE613 pKa = 3.84 QTGTKK618 pKa = 9.06 PEE620 pKa = 5.12 HH621 pKa = 6.14 ITLQDD626 pKa = 3.06 FEE628 pKa = 5.25 YY629 pKa = 9.51 PDD631 pKa = 3.53 SSGFPSRR638 pKa = 11.84 DD639 pKa = 3.09 FCLDD643 pKa = 3.98 LYY645 pKa = 11.57 SDD647 pKa = 4.68 GEE649 pKa = 4.14 DD650 pKa = 5.53 DD651 pKa = 3.7 KK652 pKa = 11.97 AGWINLFPTLSGFLDD667 pKa = 3.51 EE668 pKa = 5.73 FKK670 pKa = 10.73 RR671 pKa = 11.84 EE672 pKa = 3.52 QRR674 pKa = 11.84 VAVRR678 pKa = 11.84 QINLTVQSNDD688 pKa = 3.06 YY689 pKa = 10.36 DD690 pKa = 3.79 VKK692 pKa = 10.93 EE693 pKa = 4.37 VVGCPPPPEE702 pKa = 5.2 ANLNDD707 pKa = 3.72 EE708 pKa = 4.59 YY709 pKa = 11.66 KK710 pKa = 10.86 VFEE713 pKa = 4.29 ALKK716 pKa = 8.54 PQQVQYY722 pKa = 10.91 SEE724 pKa = 4.42 PNPRR728 pKa = 11.84 PKK730 pKa = 10.13 VVRR733 pKa = 11.84 ITEE736 pKa = 4.18 SGDD739 pKa = 3.15 IPEE742 pKa = 4.64 KK743 pKa = 10.4 FLPNMAQAVVRR754 pKa = 11.84 WLTSVGGVSQVGTVADD770 pKa = 3.72 KK771 pKa = 11.3 VGASAYY777 pKa = 10.05 QIVVGAAKK785 pKa = 10.24 GGYY788 pKa = 7.52 FTTGDD793 pKa = 3.77 EE794 pKa = 4.32 LGDD797 pKa = 4.53 LISLYY802 pKa = 10.33 PLVTPFPTLQDD813 pKa = 3.6 SQRR816 pKa = 11.84 EE817 pKa = 4.08 EE818 pKa = 4.07 MEE820 pKa = 4.21 EE821 pKa = 3.77 KK822 pKa = 10.61 RR823 pKa = 11.84 NLIDD827 pKa = 3.03 KK828 pKa = 7.4 TTAARR833 pKa = 11.84 TSALRR838 pKa = 11.84 RR839 pKa = 11.84 EE840 pKa = 4.36 IVKK843 pKa = 7.83 TQPEE847 pKa = 5.14 LINLDD852 pKa = 3.48 VAGVSNLHH860 pKa = 6.56 PPPHH864 pKa = 7.45 DD865 pKa = 3.44 INTAEE870 pKa = 4.33 DD871 pKa = 3.06 AMAYY875 pKa = 7.49 VHH877 pKa = 6.8 AMASGRR883 pKa = 11.84 FSGFTKK889 pKa = 9.95 WISEE893 pKa = 4.04 VRR895 pKa = 11.84 PNAANPVGVRR905 pKa = 11.84 LYY907 pKa = 10.75 AHH909 pKa = 6.01 SHH911 pKa = 5.06 HH912 pKa = 6.31 TQDD915 pKa = 3.78 VKK917 pKa = 11.4 LLGEE921 pKa = 4.4 AWSSSAKK928 pKa = 9.71 LAKK931 pKa = 10.11 EE932 pKa = 4.08 YY933 pKa = 10.29 ICAAILEE940 pKa = 4.73 VNNIPYY946 pKa = 9.84 KK947 pKa = 10.26 KK948 pKa = 10.43 SSFATPNVVPPPPEE962 pKa = 4.24 SSWARR967 pKa = 11.84 QVEE970 pKa = 4.8 YY971 pKa = 9.16 ATAPKK976 pKa = 9.98 AVPQIVPVHH985 pKa = 5.34 EE986 pKa = 4.29 TLDD989 pKa = 3.7 LEE991 pKa = 4.55 LFHH994 pKa = 8.92 SIMQQFPGHH1003 pKa = 6.69 CPRR1006 pKa = 11.84 QVQMLIRR1013 pKa = 11.84 EE1014 pKa = 4.32 VSHH1017 pKa = 6.91 KK1018 pKa = 11.14 NPDD1021 pKa = 3.54 DD1022 pKa = 3.54 YY1023 pKa = 10.97 RR1024 pKa = 11.84 VRR1026 pKa = 11.84 IQDD1029 pKa = 3.64 LFSRR1033 pKa = 11.84 FPPPKK1038 pKa = 9.02 VASKK1042 pKa = 10.75 RR1043 pKa = 11.84 SLLTAEE1049 pKa = 3.93 QRR1051 pKa = 11.84 TRR1053 pKa = 11.84 LNKK1056 pKa = 9.03 KK1057 pKa = 7.47 TLDD1060 pKa = 3.06 RR1061 pKa = 11.84 KK1062 pKa = 9.65 KK1063 pKa = 10.33 RR1064 pKa = 11.84 KK1065 pKa = 9.75 KK1066 pKa = 9.79 IASKK1070 pKa = 10.73 LLPTLL1075 pKa = 3.87
Molecular weight: 120.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.02
IPC2_protein 7.468
IPC_protein 7.322
Toseland 7.234
ProMoST 7.907
Dawson 8.053
Bjellqvist 8.331
Wikipedia 7.995
Rodwell 8.083
Grimsley 7.366
Solomon 8.097
Lehninger 8.112
Nozaki 8.507
DTASelect 8.156
Thurlkill 8.229
EMBOSS 8.287
Sillero 8.492
Patrickios 4.571
IPC_peptide 8.097
IPC2_peptide 7.307
IPC2.peptide.svr19 7.419
Protein with the highest isoelectric point:
>tr|C6FGJ6|C6FGJ6_9VIRU Capsid protein OS=Drosophila A virus OX=595895 PE=3 SV=1
MM1 pKa = 7.09 ATIVTTTKK9 pKa = 10.49 RR10 pKa = 11.84 ISNNSGNNFSTRR22 pKa = 11.84 NRR24 pKa = 11.84 RR25 pKa = 11.84 TPKK28 pKa = 9.22 VTVQSVSSRR37 pKa = 11.84 QIVGKK42 pKa = 9.52 NCPMVQGPRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 GRR55 pKa = 11.84 NRR57 pKa = 11.84 PRR59 pKa = 11.84 KK60 pKa = 9.57 SGSQVAYY67 pKa = 10.43 SAMPKK72 pKa = 7.91 TTLSGVDD79 pKa = 3.34 IIGVIEE85 pKa = 4.17 VQPQRR90 pKa = 11.84 AVGASLFAQAICPTAMSDD108 pKa = 4.99 SRR110 pKa = 11.84 LQQQSKK116 pKa = 10.42 LYY118 pKa = 10.65 SRR120 pKa = 11.84 WRR122 pKa = 11.84 PKK124 pKa = 10.1 KK125 pKa = 10.44 LRR127 pKa = 11.84 VLVVGSGSANTFGSLAIGWSPDD149 pKa = 3.21 PANNISGNDD158 pKa = 3.59 TQNLNRR164 pKa = 11.84 VMACRR169 pKa = 11.84 PSSMNRR175 pKa = 11.84 MNQTTVLNIPVATTRR190 pKa = 11.84 KK191 pKa = 8.93 WYY193 pKa = 8.21 LTKK196 pKa = 10.91 GNPDD200 pKa = 3.67 DD201 pKa = 5.25 ANHH204 pKa = 6.19 GFINVVVASTTGGYY218 pKa = 8.17 TGSTTFTVTLEE229 pKa = 3.87 WVIEE233 pKa = 4.12 FEE235 pKa = 4.14 GAEE238 pKa = 4.12 LGAVSIDD245 pKa = 3.56 EE246 pKa = 6.03 DD247 pKa = 3.68 ITPDD251 pKa = 3.56 TGFQDD256 pKa = 5.11 LFTTSDD262 pKa = 3.64 GSWNSEE268 pKa = 3.85 YY269 pKa = 10.44 LTMKK273 pKa = 8.65 MHH275 pKa = 7.22 HH276 pKa = 6.95 GGQMVPFSAAHH287 pKa = 4.42 QAYY290 pKa = 9.92 VYY292 pKa = 10.43 TNAPGTNVTYY302 pKa = 10.7 YY303 pKa = 11.08 LEE305 pKa = 5.49 DD306 pKa = 3.31 KK307 pKa = 8.03 TTLKK311 pKa = 9.55 TANFFAVIQGYY322 pKa = 8.8 SIPGFVMFASYY333 pKa = 10.71 TDD335 pKa = 3.13 AKK337 pKa = 11.04 DD338 pKa = 3.58 YY339 pKa = 11.16 IRR341 pKa = 11.84 TGDD344 pKa = 3.12 TGKK347 pKa = 10.37 CLKK350 pKa = 10.4 YY351 pKa = 8.72 YY352 pKa = 8.93 TQGPVISPPIPKK364 pKa = 9.66 FRR366 pKa = 11.84 VADD369 pKa = 3.71 EE370 pKa = 4.24 AVQTVEE376 pKa = 4.01 TNLLRR381 pKa = 11.84 RR382 pKa = 11.84 IDD384 pKa = 3.63 QLTEE388 pKa = 3.26 QLRR391 pKa = 11.84 AVNAPVTRR399 pKa = 11.84 TVALSKK405 pKa = 10.09 DD406 pKa = 3.27 TAKK409 pKa = 10.34 IRR411 pKa = 11.84 TKK413 pKa = 10.96 LEE415 pKa = 3.76 GMPDD419 pKa = 3.54 GTTLSPFKK427 pKa = 10.79 VISEE431 pKa = 4.55 DD432 pKa = 3.28 PVAAQDD438 pKa = 3.73 FEE440 pKa = 4.65 IVV442 pKa = 3.15
Molecular weight: 48.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.878
IPC2_protein 8.946
IPC_protein 8.887
Toseland 9.487
ProMoST 9.311
Dawson 9.794
Bjellqvist 9.531
Wikipedia 9.984
Rodwell 10.014
Grimsley 9.867
Solomon 9.823
Lehninger 9.78
Nozaki 9.589
DTASelect 9.487
Thurlkill 9.619
EMBOSS 9.926
Sillero 9.721
Patrickios 4.991
IPC_peptide 9.809
IPC2_peptide 8.2
IPC2.peptide.svr19 7.875
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1517
442
1075
758.5
84.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.987 ± 0.243
1.055 ± 0.076
5.274 ± 0.265
5.274 ± 1.069
3.164 ± 0.231
6.592 ± 0.329
2.373 ± 0.745
5.208 ± 0.002
5.801 ± 0.533
7.383 ± 0.992
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.637 ± 0.04
3.955 ± 0.864
6.658 ± 0.509
4.548 ± 0.103
5.933 ± 0.089
6.328 ± 0.807
7.515 ± 1.698
8.833 ± 0.12
1.516 ± 0.195
2.966 ± 0.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here