Drosophila A virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 7.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C6FGJ6|C6FGJ6_9VIRU Capsid protein OS=Drosophila A virus OX=595895 PE=3 SV=1
MM1 pKa = 7.89DD2 pKa = 5.63ASNPIISSDD11 pKa = 3.13RR12 pKa = 11.84STVNQVYY19 pKa = 10.8AKK21 pKa = 8.34MVRR24 pKa = 11.84RR25 pKa = 11.84KK26 pKa = 9.91RR27 pKa = 11.84QDD29 pKa = 3.14LAYY32 pKa = 9.99LQEE35 pKa = 4.49LVRR38 pKa = 11.84TAHH41 pKa = 7.52RR42 pKa = 11.84EE43 pKa = 3.66ILPQGEE49 pKa = 4.6LPHH52 pKa = 6.55EE53 pKa = 4.74SEE55 pKa = 4.14AKK57 pKa = 10.34QYY59 pKa = 9.04VAEE62 pKa = 4.26YY63 pKa = 9.9KK64 pKa = 10.69ARR66 pKa = 11.84LPVVDD71 pKa = 3.8TKK73 pKa = 11.25KK74 pKa = 10.75VGEE77 pKa = 4.42LPTEE81 pKa = 4.42VFQVALPSIPVRR93 pKa = 11.84GIMDD97 pKa = 4.02AVGVPLHH104 pKa = 6.6PPSINNRR111 pKa = 11.84GGHH114 pKa = 4.3THH116 pKa = 5.49VTRR119 pKa = 11.84QMGGVPHH126 pKa = 7.35PPLGLIPIAQKK137 pKa = 10.91YY138 pKa = 8.65GVEE141 pKa = 4.08ILDD144 pKa = 3.53QKK146 pKa = 10.86HH147 pKa = 6.03VYY149 pKa = 8.85TGGTWDD155 pKa = 3.84GFLSRR160 pKa = 11.84LAQQMGRR167 pKa = 11.84TTVPLARR174 pKa = 11.84TVSAGKK180 pKa = 10.43KK181 pKa = 8.46NASDD185 pKa = 3.42RR186 pKa = 11.84AIIKK190 pKa = 9.68LLNKK194 pKa = 9.81YY195 pKa = 7.53MPRR198 pKa = 11.84TGNKK202 pKa = 8.54SWPGVHH208 pKa = 5.45TTPEE212 pKa = 3.9EE213 pKa = 4.79GILDD217 pKa = 4.91GIKK220 pKa = 8.85ITAKK224 pKa = 10.21SSAGAPYY231 pKa = 8.86WRR233 pKa = 11.84HH234 pKa = 5.72KK235 pKa = 10.91GEE237 pKa = 4.49CLDD240 pKa = 4.83HH241 pKa = 8.26IIDD244 pKa = 3.7TGLPVVLKK252 pKa = 10.22HH253 pKa = 6.23IKK255 pKa = 10.24EE256 pKa = 4.25GTLNQLWRR264 pKa = 11.84EE265 pKa = 4.0NPEE268 pKa = 3.83MFLVEE273 pKa = 4.51VKK275 pKa = 10.91NKK277 pKa = 9.66LDD279 pKa = 3.43RR280 pKa = 11.84YY281 pKa = 9.18EE282 pKa = 3.95VSKK285 pKa = 11.07LKK287 pKa = 10.71EE288 pKa = 3.48KK289 pKa = 9.57TRR291 pKa = 11.84PYY293 pKa = 10.4VCVPAHH299 pKa = 5.87WALLFSCLTQGFQEE313 pKa = 4.45GLQVFSNLDD322 pKa = 3.42PSTEE326 pKa = 3.95CSNAYY331 pKa = 9.84GFSSIAGGLTRR342 pKa = 11.84MVDD345 pKa = 2.61WMYY348 pKa = 11.39ACPKK352 pKa = 9.33RR353 pKa = 11.84RR354 pKa = 11.84GRR356 pKa = 11.84VVCYY360 pKa = 10.63GDD362 pKa = 3.82DD363 pKa = 3.4ACITFWSQGVLYY375 pKa = 10.45RR376 pKa = 11.84VDD378 pKa = 3.88PDD380 pKa = 4.19FKK382 pKa = 11.51QMDD385 pKa = 3.82GSIDD389 pKa = 3.76RR390 pKa = 11.84EE391 pKa = 4.05DD392 pKa = 3.42ARR394 pKa = 11.84ITIEE398 pKa = 3.99WVLHH402 pKa = 5.56HH403 pKa = 6.5LRR405 pKa = 11.84KK406 pKa = 10.16DD407 pKa = 3.37LGVEE411 pKa = 3.84EE412 pKa = 5.43TPAFWKK418 pKa = 9.32TVAAVWLDD426 pKa = 3.59MAIDD430 pKa = 3.33PHH432 pKa = 7.58FIVDD436 pKa = 4.2GKK438 pKa = 8.74TVYY441 pKa = 10.03KK442 pKa = 10.36KK443 pKa = 10.64KK444 pKa = 10.35NPHH447 pKa = 5.6GLMTGVPGTTLFDD460 pKa = 3.7TVKK463 pKa = 10.86SVIVWNEE470 pKa = 3.52MLDD473 pKa = 3.65QASVGSIDD481 pKa = 4.79LLNEE485 pKa = 3.99AQVVEE490 pKa = 4.08WMKK493 pKa = 10.86RR494 pKa = 11.84QGLVVKK500 pKa = 9.81EE501 pKa = 4.52GTWSPVALPARR512 pKa = 11.84DD513 pKa = 3.81TEE515 pKa = 4.8GLITDD520 pKa = 4.16HH521 pKa = 6.99KK522 pKa = 10.45FLGVQIMGVYY532 pKa = 9.12HH533 pKa = 6.02RR534 pKa = 11.84HH535 pKa = 5.26RR536 pKa = 11.84VIHH539 pKa = 6.02VPTMPEE545 pKa = 3.56SDD547 pKa = 4.04ALEE550 pKa = 4.0MMLCQKK556 pKa = 10.76DD557 pKa = 3.78NPFEE561 pKa = 4.23KK562 pKa = 10.44AVSRR566 pKa = 11.84TARR569 pKa = 11.84QRR571 pKa = 11.84TLYY574 pKa = 11.04DD575 pKa = 3.18RR576 pKa = 11.84MRR578 pKa = 11.84GLMITMGFSIPRR590 pKa = 11.84IEE592 pKa = 4.24EE593 pKa = 3.99TIHH596 pKa = 6.74AVVNTIPGEE605 pKa = 4.12IIVMQTQEE613 pKa = 3.84QTGTKK618 pKa = 9.06PEE620 pKa = 5.12HH621 pKa = 6.14ITLQDD626 pKa = 3.06FEE628 pKa = 5.25YY629 pKa = 9.51PDD631 pKa = 3.53SSGFPSRR638 pKa = 11.84DD639 pKa = 3.09FCLDD643 pKa = 3.98LYY645 pKa = 11.57SDD647 pKa = 4.68GEE649 pKa = 4.14DD650 pKa = 5.53DD651 pKa = 3.7KK652 pKa = 11.97AGWINLFPTLSGFLDD667 pKa = 3.51EE668 pKa = 5.73FKK670 pKa = 10.73RR671 pKa = 11.84EE672 pKa = 3.52QRR674 pKa = 11.84VAVRR678 pKa = 11.84QINLTVQSNDD688 pKa = 3.06YY689 pKa = 10.36DD690 pKa = 3.79VKK692 pKa = 10.93EE693 pKa = 4.37VVGCPPPPEE702 pKa = 5.2ANLNDD707 pKa = 3.72EE708 pKa = 4.59YY709 pKa = 11.66KK710 pKa = 10.86VFEE713 pKa = 4.29ALKK716 pKa = 8.54PQQVQYY722 pKa = 10.91SEE724 pKa = 4.42PNPRR728 pKa = 11.84PKK730 pKa = 10.13VVRR733 pKa = 11.84ITEE736 pKa = 4.18SGDD739 pKa = 3.15IPEE742 pKa = 4.64KK743 pKa = 10.4FLPNMAQAVVRR754 pKa = 11.84WLTSVGGVSQVGTVADD770 pKa = 3.72KK771 pKa = 11.3VGASAYY777 pKa = 10.05QIVVGAAKK785 pKa = 10.24GGYY788 pKa = 7.52FTTGDD793 pKa = 3.77EE794 pKa = 4.32LGDD797 pKa = 4.53LISLYY802 pKa = 10.33PLVTPFPTLQDD813 pKa = 3.6SQRR816 pKa = 11.84EE817 pKa = 4.08EE818 pKa = 4.07MEE820 pKa = 4.21EE821 pKa = 3.77KK822 pKa = 10.61RR823 pKa = 11.84NLIDD827 pKa = 3.03KK828 pKa = 7.4TTAARR833 pKa = 11.84TSALRR838 pKa = 11.84RR839 pKa = 11.84EE840 pKa = 4.36IVKK843 pKa = 7.83TQPEE847 pKa = 5.14LINLDD852 pKa = 3.48VAGVSNLHH860 pKa = 6.56PPPHH864 pKa = 7.45DD865 pKa = 3.44INTAEE870 pKa = 4.33DD871 pKa = 3.06AMAYY875 pKa = 7.49VHH877 pKa = 6.8AMASGRR883 pKa = 11.84FSGFTKK889 pKa = 9.95WISEE893 pKa = 4.04VRR895 pKa = 11.84PNAANPVGVRR905 pKa = 11.84LYY907 pKa = 10.75AHH909 pKa = 6.01SHH911 pKa = 5.06HH912 pKa = 6.31TQDD915 pKa = 3.78VKK917 pKa = 11.4LLGEE921 pKa = 4.4AWSSSAKK928 pKa = 9.71LAKK931 pKa = 10.11EE932 pKa = 4.08YY933 pKa = 10.29ICAAILEE940 pKa = 4.73VNNIPYY946 pKa = 9.84KK947 pKa = 10.26KK948 pKa = 10.43SSFATPNVVPPPPEE962 pKa = 4.24SSWARR967 pKa = 11.84QVEE970 pKa = 4.8YY971 pKa = 9.16ATAPKK976 pKa = 9.98AVPQIVPVHH985 pKa = 5.34EE986 pKa = 4.29TLDD989 pKa = 3.7LEE991 pKa = 4.55LFHH994 pKa = 8.92SIMQQFPGHH1003 pKa = 6.69CPRR1006 pKa = 11.84QVQMLIRR1013 pKa = 11.84EE1014 pKa = 4.32VSHH1017 pKa = 6.91KK1018 pKa = 11.14NPDD1021 pKa = 3.54DD1022 pKa = 3.54YY1023 pKa = 10.97RR1024 pKa = 11.84VRR1026 pKa = 11.84IQDD1029 pKa = 3.64LFSRR1033 pKa = 11.84FPPPKK1038 pKa = 9.02VASKK1042 pKa = 10.75RR1043 pKa = 11.84SLLTAEE1049 pKa = 3.93QRR1051 pKa = 11.84TRR1053 pKa = 11.84LNKK1056 pKa = 9.03KK1057 pKa = 7.47TLDD1060 pKa = 3.06RR1061 pKa = 11.84KK1062 pKa = 9.65KK1063 pKa = 10.33RR1064 pKa = 11.84KK1065 pKa = 9.75KK1066 pKa = 9.79IASKK1070 pKa = 10.73LLPTLL1075 pKa = 3.87

Molecular weight:
120.57 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|C6FGJ6|C6FGJ6_9VIRU Capsid protein OS=Drosophila A virus OX=595895 PE=3 SV=1
MM1 pKa = 7.09ATIVTTTKK9 pKa = 10.49RR10 pKa = 11.84ISNNSGNNFSTRR22 pKa = 11.84NRR24 pKa = 11.84RR25 pKa = 11.84TPKK28 pKa = 9.22VTVQSVSSRR37 pKa = 11.84QIVGKK42 pKa = 9.52NCPMVQGPRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84GRR55 pKa = 11.84NRR57 pKa = 11.84PRR59 pKa = 11.84KK60 pKa = 9.57SGSQVAYY67 pKa = 10.43SAMPKK72 pKa = 7.91TTLSGVDD79 pKa = 3.34IIGVIEE85 pKa = 4.17VQPQRR90 pKa = 11.84AVGASLFAQAICPTAMSDD108 pKa = 4.99SRR110 pKa = 11.84LQQQSKK116 pKa = 10.42LYY118 pKa = 10.65SRR120 pKa = 11.84WRR122 pKa = 11.84PKK124 pKa = 10.1KK125 pKa = 10.44LRR127 pKa = 11.84VLVVGSGSANTFGSLAIGWSPDD149 pKa = 3.21PANNISGNDD158 pKa = 3.59TQNLNRR164 pKa = 11.84VMACRR169 pKa = 11.84PSSMNRR175 pKa = 11.84MNQTTVLNIPVATTRR190 pKa = 11.84KK191 pKa = 8.93WYY193 pKa = 8.21LTKK196 pKa = 10.91GNPDD200 pKa = 3.67DD201 pKa = 5.25ANHH204 pKa = 6.19GFINVVVASTTGGYY218 pKa = 8.17TGSTTFTVTLEE229 pKa = 3.87WVIEE233 pKa = 4.12FEE235 pKa = 4.14GAEE238 pKa = 4.12LGAVSIDD245 pKa = 3.56EE246 pKa = 6.03DD247 pKa = 3.68ITPDD251 pKa = 3.56TGFQDD256 pKa = 5.11LFTTSDD262 pKa = 3.64GSWNSEE268 pKa = 3.85YY269 pKa = 10.44LTMKK273 pKa = 8.65MHH275 pKa = 7.22HH276 pKa = 6.95GGQMVPFSAAHH287 pKa = 4.42QAYY290 pKa = 9.92VYY292 pKa = 10.43TNAPGTNVTYY302 pKa = 10.7YY303 pKa = 11.08LEE305 pKa = 5.49DD306 pKa = 3.31KK307 pKa = 8.03TTLKK311 pKa = 9.55TANFFAVIQGYY322 pKa = 8.8SIPGFVMFASYY333 pKa = 10.71TDD335 pKa = 3.13AKK337 pKa = 11.04DD338 pKa = 3.58YY339 pKa = 11.16IRR341 pKa = 11.84TGDD344 pKa = 3.12TGKK347 pKa = 10.37CLKK350 pKa = 10.4YY351 pKa = 8.72YY352 pKa = 8.93TQGPVISPPIPKK364 pKa = 9.66FRR366 pKa = 11.84VADD369 pKa = 3.71EE370 pKa = 4.24AVQTVEE376 pKa = 4.01TNLLRR381 pKa = 11.84RR382 pKa = 11.84IDD384 pKa = 3.63QLTEE388 pKa = 3.26QLRR391 pKa = 11.84AVNAPVTRR399 pKa = 11.84TVALSKK405 pKa = 10.09DD406 pKa = 3.27TAKK409 pKa = 10.34IRR411 pKa = 11.84TKK413 pKa = 10.96LEE415 pKa = 3.76GMPDD419 pKa = 3.54GTTLSPFKK427 pKa = 10.79VISEE431 pKa = 4.55DD432 pKa = 3.28PVAAQDD438 pKa = 3.73FEE440 pKa = 4.65IVV442 pKa = 3.15

Molecular weight:
48.39 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1517

442

1075

758.5

84.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.987 ± 0.243

1.055 ± 0.076

5.274 ± 0.265

5.274 ± 1.069

3.164 ± 0.231

6.592 ± 0.329

2.373 ± 0.745

5.208 ± 0.002

5.801 ± 0.533

7.383 ± 0.992

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.04

3.955 ± 0.864

6.658 ± 0.509

4.548 ± 0.103

5.933 ± 0.089

6.328 ± 0.807

7.515 ± 1.698

8.833 ± 0.12

1.516 ± 0.195

2.966 ± 0.102

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski