Paenibacillus prosopidis
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6048 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A368VRC7|A0A368VRC7_9BACL Uncharacterized protein OS=Paenibacillus prosopidis OX=630520 GN=DFP97_11480 PE=4 SV=1
MM1 pKa = 7.24 NKK3 pKa = 9.48 IMMVLTLILLLSGCSAANNNVGNTPDD29 pKa = 3.47 NSNPSTNVGQGNQPDD44 pKa = 3.98 PSEE47 pKa = 4.28 PAPEE51 pKa = 4.06 EE52 pKa = 3.84 TDD54 pKa = 2.93 TSNKK58 pKa = 9.53 VEE60 pKa = 4.21 EE61 pKa = 4.5 EE62 pKa = 4.31 EE63 pKa = 4.28 EE64 pKa = 3.87 PAAATTEE71 pKa = 4.1 QEE73 pKa = 3.77 AAEE76 pKa = 4.06 AVILALKK83 pKa = 8.85 EE84 pKa = 3.91 ADD86 pKa = 3.5 MTALAAYY93 pKa = 8.64 IHH95 pKa = 7.23 PDD97 pKa = 2.94 KK98 pKa = 11.3 GLLFSPYY105 pKa = 10.82 AHH107 pKa = 7.53 IDD109 pKa = 3.32 TASAKK114 pKa = 9.48 VFPAAGLPALTDD126 pKa = 3.39 PTVYY130 pKa = 9.25 TWGSYY135 pKa = 10.87 DD136 pKa = 3.43 GTGDD140 pKa = 4.22 PIEE143 pKa = 4.72 LTFQQYY149 pKa = 9.66 FDD151 pKa = 3.72 KK152 pKa = 10.91 FVYY155 pKa = 10.51 DD156 pKa = 3.66 KK157 pKa = 11.56 DD158 pKa = 4.18 FIEE161 pKa = 5.18 PEE163 pKa = 4.24 TIGVDD168 pKa = 3.76 EE169 pKa = 5.05 IKK171 pKa = 11.18 GEE173 pKa = 4.21 GNTLVNIKK181 pKa = 10.5 DD182 pKa = 3.86 VFPNSYY188 pKa = 10.91 VMDD191 pKa = 4.0 YY192 pKa = 10.64 YY193 pKa = 11.29 FSGFDD198 pKa = 3.23 AQYY201 pKa = 11.55 NGMDD205 pKa = 3.72 WEE207 pKa = 4.3 SLILVLEE214 pKa = 4.38 EE215 pKa = 5.87 LDD217 pKa = 3.89 GAWYY221 pKa = 10.36 VSAIVHH227 pKa = 5.25 SQWTII232 pKa = 2.91
Molecular weight: 25.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.592
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.617
Grimsley 3.503
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.062
Thurlkill 3.63
EMBOSS 3.694
Sillero 3.91
Patrickios 0.807
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.812
Protein with the highest isoelectric point:
>tr|A0A368VMV4|A0A368VMV4_9BACL Uncharacterized protein OS=Paenibacillus prosopidis OX=630520 GN=DFP97_114113 PE=4 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 PTFKK6 pKa = 10.84 PNVSKK11 pKa = 10.73 RR12 pKa = 11.84 AKK14 pKa = 8.72 VHH16 pKa = 5.43 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 10.18 NGRR28 pKa = 11.84 KK29 pKa = 8.78 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 QRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.84 VLSAA44 pKa = 4.11
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.676
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.398
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6048
0
6048
1908774
26
2783
315.6
35.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.075 ± 0.04
0.754 ± 0.009
5.195 ± 0.025
6.642 ± 0.034
4.283 ± 0.024
7.326 ± 0.029
2.074 ± 0.015
6.974 ± 0.025
5.487 ± 0.027
9.812 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.804 ± 0.017
4.169 ± 0.025
3.978 ± 0.021
3.783 ± 0.02
4.876 ± 0.029
6.344 ± 0.027
5.49 ± 0.031
6.943 ± 0.026
1.377 ± 0.012
3.614 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here