Clonorchis sinensis (Chinese liver fluke)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda; Digenea; Opisthorchiida; Opisthorchiata; Opisthorchiidae; Clonorchis

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3R7EPN6|A0A3R7EPN6_CLOSI Alpha-ketoglutarate-dependent dioxygenase alkB-like protein 7 OS=Clonorchis sinensis OX=79923 GN=ALKBH7 PE=4 SV=1
MM1 pKa = 7.54NSNDD5 pKa = 3.93LTDD8 pKa = 3.81HH9 pKa = 6.38QIDD12 pKa = 3.86MLQHH16 pKa = 6.3NDD18 pKa = 2.88VNQFDD23 pKa = 4.21SSDD26 pKa = 3.85PNSVGSNAPMVSSVWLEE43 pKa = 3.6QGDD46 pKa = 4.18YY47 pKa = 11.14FFDD50 pKa = 4.88DD51 pKa = 4.1EE52 pKa = 5.14FSVPFDD58 pKa = 3.91TNLQDD63 pKa = 5.9DD64 pKa = 4.84LVQNEE69 pKa = 4.32LNMNGMTVSLGPTVPPSRR87 pKa = 11.84TNLQKK92 pKa = 10.66LRR94 pKa = 11.84PTFVYY99 pKa = 10.03TLLGYY104 pKa = 10.27FLFACICRR112 pKa = 11.84TACEE116 pKa = 4.24KK117 pKa = 10.88SQSLDD122 pKa = 3.38CC123 pKa = 5.63

Molecular weight: 13.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A419PQ31|A0A419PQ31_CLOSI Neuronal acetylcholine receptor subunit alpha-4 (Fragment) OS=Clonorchis sinensis OX=79923 GN=Chrna4 PE=4 SV=1
MM1 pKa = 7.5LPLMMMMMMMLMMMVMMMMRR21 pKa = 11.84MMMMMMMRR29 pKa = 11.84MMMMMMMKK37 pKa = 9.62MMVMMMMRR45 pKa = 11.84MMMMMMMMKK54 pKa = 9.54MMMMMMMLMMMVMMMMRR71 pKa = 11.84MMMMMMMRR79 pKa = 11.84MMMMMMMKK87 pKa = 9.54MMMMMMMLMMMVMMMMVMMVMMMRR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84MTMMMMMMTMMMMMMVMMVMMMRR137 pKa = 11.84RR138 pKa = 11.84RR139 pKa = 11.84RR140 pKa = 11.84MTMMMMMMVMMMMVMMMMMMVMMVMMMRR168 pKa = 11.84RR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 11.84MTMMMMMMTMMTMMMVVACC190 pKa = 4.7

Molecular weight: 24.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14505

0

14505

6939493

45

9462

478.4

53.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.734 ± 0.018

2.25 ± 0.011

5.007 ± 0.022

5.647 ± 0.026

3.809 ± 0.016

5.588 ± 0.023

2.798 ± 0.01

4.609 ± 0.021

4.417 ± 0.016

9.909 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.046 ± 0.013

4.04 ± 0.012

5.971 ± 0.025

4.31 ± 0.014

6.809 ± 0.017

9.383 ± 0.031

6.412 ± 0.016

6.373 ± 0.016

1.274 ± 0.011

2.599 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski