Apis mellifera associated microvirus 49

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UU17|A0A3S8UU17_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 49 OX=2494779 PE=4 SV=1
MM1 pKa = 7.28SEE3 pKa = 4.38KK4 pKa = 10.21IRR6 pKa = 11.84VEE8 pKa = 3.68VRR10 pKa = 11.84PEE12 pKa = 3.65VGQVDD17 pKa = 4.91LEE19 pKa = 4.39DD20 pKa = 4.39LLAEE24 pKa = 4.41AGKK27 pKa = 10.67AEE29 pKa = 4.08WSDD32 pKa = 3.9DD33 pKa = 3.73PGTGKK38 pKa = 10.41AVLLRR43 pKa = 11.84DD44 pKa = 3.6EE45 pKa = 5.17RR46 pKa = 11.84GNPAYY51 pKa = 9.45EE52 pKa = 3.97VHH54 pKa = 6.54NPLPHH59 pKa = 6.74APPIGFQPTPPIEE72 pKa = 3.85EE73 pKa = 5.11LIRR76 pKa = 11.84DD77 pKa = 3.74RR78 pKa = 11.84VRR80 pKa = 11.84AEE82 pKa = 3.78FARR85 pKa = 11.84LRR87 pKa = 11.84DD88 pKa = 3.45EE89 pKa = 5.74DD90 pKa = 4.75EE91 pKa = 4.13IDD93 pKa = 3.59SAEE96 pKa = 4.1EE97 pKa = 3.65VDD99 pKa = 5.76DD100 pKa = 5.04FDD102 pKa = 6.16VPDD105 pKa = 4.47EE106 pKa = 4.63LPLASVYY113 pKa = 10.56EE114 pKa = 4.26VVQMEE119 pKa = 4.47DD120 pKa = 3.03TAPRR124 pKa = 11.84PPAAEE129 pKa = 3.69LSMEE133 pKa = 4.04EE134 pKa = 4.19RR135 pKa = 11.84ARR137 pKa = 11.84MHH139 pKa = 6.46VDD141 pKa = 3.92FMEE144 pKa = 4.25LAEE147 pKa = 4.35RR148 pKa = 11.84EE149 pKa = 3.99RR150 pKa = 11.84LNRR153 pKa = 11.84KK154 pKa = 7.96RR155 pKa = 11.84AKK157 pKa = 9.28EE158 pKa = 3.55AHH160 pKa = 6.17LAKK163 pKa = 10.24QRR165 pKa = 11.84EE166 pKa = 4.35AVKK169 pKa = 10.38AAEE172 pKa = 4.44EE173 pKa = 4.06DD174 pKa = 3.73VALYY178 pKa = 8.73GAPPAEE184 pKa = 4.39PQSS187 pKa = 3.66

Molecular weight: 21.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTZ3|A0A3S8UTZ3_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 49 OX=2494779 PE=3 SV=1
MM1 pKa = 7.33IVLPRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84EE9 pKa = 3.99RR10 pKa = 11.84IRR12 pKa = 11.84LARR15 pKa = 11.84LAPAVSMGRR24 pKa = 11.84PVSPAGRR31 pKa = 11.84PFFQILRR38 pKa = 11.84PKK40 pKa = 9.04VTLTLIEE47 pKa = 4.16DD48 pKa = 3.6RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84FHH53 pKa = 7.14PLGKK57 pKa = 9.88AAPAAVISRR66 pKa = 11.84RR67 pKa = 11.84DD68 pKa = 3.07QRR70 pKa = 11.84RR71 pKa = 11.84IVEE74 pKa = 4.18KK75 pKa = 10.58VKK77 pKa = 10.86DD78 pKa = 3.68VSRR81 pKa = 11.84YY82 pKa = 9.5AGPLAMPVARR92 pKa = 11.84LGFAVPSKK100 pKa = 10.58VAVCVRR106 pKa = 11.84RR107 pKa = 11.84KK108 pKa = 7.7QRR110 pKa = 11.84RR111 pKa = 11.84EE112 pKa = 3.68AIFAFGRR119 pKa = 11.84AGKK122 pKa = 9.76GAAAKK127 pKa = 9.91RR128 pKa = 11.84RR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84SEE133 pKa = 3.82FTNISCC139 pKa = 5.38

Molecular weight: 15.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1377

139

493

275.4

30.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.111 ± 1.328

1.162 ± 0.703

5.156 ± 0.644

5.81 ± 1.449

3.486 ± 0.714

7.407 ± 1.375

1.888 ± 0.382

4.575 ± 0.292

3.849 ± 0.92

8.134 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.358

3.195 ± 0.534

6.536 ± 0.625

3.776 ± 0.709

9.296 ± 1.808

6.609 ± 1.335

5.229 ± 1.246

6.173 ± 0.701

1.525 ± 0.352

2.832 ± 0.539
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski