Streptococcus phage Javan629

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AN19|A0A4D6AN19_9CAUD Uncharacterized protein OS=Streptococcus phage Javan629 OX=2548288 GN=Javan629_0014 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 9.33ITWTGQPTNKK12 pKa = 9.54VVTYY16 pKa = 10.24KK17 pKa = 10.32IEE19 pKa = 4.33ADD21 pKa = 3.51SYY23 pKa = 11.72KK24 pKa = 10.67EE25 pKa = 3.73ILEE28 pKa = 3.99QMIDD32 pKa = 3.35RR33 pKa = 11.84DD34 pKa = 4.06LADD37 pKa = 5.24GYY39 pKa = 10.32WNPDD43 pKa = 2.68SQTFHH48 pKa = 6.71EE49 pKa = 4.47LAYY52 pKa = 9.32YY53 pKa = 10.35SPIISEE59 pKa = 5.89LEE61 pKa = 3.84DD62 pKa = 3.79EE63 pKa = 4.81YY64 pKa = 12.02NNLDD68 pKa = 4.79DD69 pKa = 6.3SDD71 pKa = 6.19DD72 pKa = 3.54EE73 pKa = 4.65DD74 pKa = 4.35AFEE77 pKa = 5.95KK78 pKa = 10.65FDD80 pKa = 3.71EE81 pKa = 4.47KK82 pKa = 11.05LGNVEE87 pKa = 3.75WNEE90 pKa = 3.87ILNSLSDD97 pKa = 3.64NEE99 pKa = 4.17MEE101 pKa = 4.8QVIRR105 pKa = 11.84GCNSQAYY112 pKa = 7.86YY113 pKa = 10.76QEE115 pKa = 4.88FEE117 pKa = 4.16VSEE120 pKa = 4.21

Molecular weight: 14.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AN48|A0A4D6AN48_9CAUD AAA_23 domain-containing protein OS=Streptococcus phage Javan629 OX=2548288 GN=Javan629_0044 PE=4 SV=1
MM1 pKa = 7.25IRR3 pKa = 11.84VPQKK7 pKa = 9.24DD8 pKa = 3.61TSPVFSKK15 pKa = 11.08NSDD18 pKa = 3.44YY19 pKa = 11.33LKK21 pKa = 10.59QKK23 pKa = 9.73EE24 pKa = 4.31AEE26 pKa = 4.24KK27 pKa = 10.73KK28 pKa = 10.66LKK30 pKa = 9.72FLKK33 pKa = 10.48GKK35 pKa = 10.45RR36 pKa = 11.84GG37 pKa = 3.31

Molecular weight: 4.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11962

37

1378

234.5

26.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.73 ± 0.447

0.485 ± 0.111

6.529 ± 0.322

7.449 ± 0.513

3.921 ± 0.339

6.362 ± 0.422

1.187 ± 0.104

6.964 ± 0.314

8.636 ± 0.477

8.351 ± 0.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.166

5.618 ± 0.219

2.826 ± 0.204

4.205 ± 0.311

3.687 ± 0.328

6.746 ± 0.465

6.83 ± 0.52

6.186 ± 0.277

1.304 ± 0.122

3.586 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski