Capybara microvirus Cap3_SP_320
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5M5|A0A4P8W5M5_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_320 OX=2585428 PE=4 SV=1
MM1 pKa = 7.63 PSSSRR6 pKa = 11.84 SPSVEE11 pKa = 3.53 LVRR14 pKa = 11.84 PFVFSSRR21 pKa = 11.84 PLDD24 pKa = 4.21 DD25 pKa = 5.16 SDD27 pKa = 4.57 FFCSSVYY34 pKa = 10.77 DD35 pKa = 4.36 RR36 pKa = 11.84 IVDD39 pKa = 3.61 DD40 pKa = 3.91 TDD42 pKa = 4.11 FVSSSSEE49 pKa = 3.47 NRR51 pKa = 11.84 VQFLSQSLGAGSGPGLYY68 pKa = 10.4 DD69 pKa = 3.69 FSKK72 pKa = 10.89 PSDD75 pKa = 3.5 FDD77 pKa = 3.87 LEE79 pKa = 4.16 NFEE82 pKa = 5.84 RR83 pKa = 11.84 IDD85 pKa = 3.55 KK86 pKa = 10.4 GLSSMVLALRR96 pKa = 11.84 DD97 pKa = 3.84 GKK99 pKa = 9.25 LTPDD103 pKa = 5.06 DD104 pKa = 3.87 ISTLRR109 pKa = 11.84 TKK111 pKa = 11.14 LMDD114 pKa = 3.68 AVEE117 pKa = 4.31 HH118 pKa = 6.36 SKK120 pKa = 10.9 SEE122 pKa = 4.09 HH123 pKa = 5.07 EE124 pKa = 4.17 RR125 pKa = 11.84 ALAEE129 pKa = 3.91 QALEE133 pKa = 4.07 EE134 pKa = 4.34 VVAARR139 pKa = 11.84 QSHH142 pKa = 6.39 LDD144 pKa = 3.17 KK145 pKa = 10.9 STGFEE150 pKa = 4.07 PPDD153 pKa = 3.41 VSKK156 pKa = 11.34 SS157 pKa = 3.37
Molecular weight: 17.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.682
IPC2_protein 4.558
IPC_protein 4.495
Toseland 4.329
ProMoST 4.635
Dawson 4.469
Bjellqvist 4.622
Wikipedia 4.38
Rodwell 4.342
Grimsley 4.24
Solomon 4.469
Lehninger 4.418
Nozaki 4.584
DTASelect 4.8
Thurlkill 4.342
EMBOSS 4.393
Sillero 4.622
Patrickios 4.228
IPC_peptide 4.469
IPC2_peptide 4.609
IPC2.peptide.svr19 4.568
Protein with the highest isoelectric point:
>tr|A0A4P8W548|A0A4P8W548_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_320 OX=2585428 PE=4 SV=1
MM1 pKa = 7.27 FLILFHH7 pKa = 6.56 FEE9 pKa = 4.52 KK10 pKa = 10.79 KK11 pKa = 10.69 LLDD14 pKa = 3.22 IFLFLLYY21 pKa = 10.51 YY22 pKa = 11.01 LFMPLIFGIISSLIQTRR39 pKa = 11.84 EE40 pKa = 3.65 SNMRR44 pKa = 11.84 GGSTAPVLASGVSDD58 pKa = 3.19 ILKK61 pKa = 10.66 SLGGTKK67 pKa = 10.12
Molecular weight: 7.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.958
IPC2_protein 8.726
IPC_protein 8.873
Toseland 9.428
ProMoST 9.443
Dawson 9.648
Bjellqvist 9.297
Wikipedia 9.823
Rodwell 9.94
Grimsley 9.721
Solomon 9.823
Lehninger 9.794
Nozaki 9.326
DTASelect 9.326
Thurlkill 9.472
EMBOSS 9.809
Sillero 9.56
Patrickios 7.629
IPC_peptide 9.809
IPC2_peptide 7.922
IPC2.peptide.svr19 7.798
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
1833
67
668
203.7
23.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.183 ± 0.863
1.964 ± 0.51
7.583 ± 0.729
4.364 ± 0.408
7.038 ± 1.049
5.401 ± 0.774
2.073 ± 0.428
5.074 ± 0.729
4.364 ± 0.345
9.22 ± 0.686
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.8 ± 0.366
4.201 ± 0.729
4.474 ± 0.583
2.837 ± 0.693
6.438 ± 0.722
11.511 ± 0.725
3.873 ± 0.54
6.383 ± 0.95
0.818 ± 0.338
5.401 ± 0.683
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here