Bacteroides fluxus YIT 12057
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3920 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F3PQ80|F3PQ80_9BACE OmpR/PhoB-type domain-containing protein OS=Bacteroides fluxus YIT 12057 OX=763034 GN=HMPREF9446_00872 PE=4 SV=1
MM1 pKa = 7.96 KK2 pKa = 9.31 ITTEE6 pKa = 3.66 MARR9 pKa = 11.84 YY10 pKa = 7.8 IWSIIAANPSLSMSWGVDD28 pKa = 2.69 TDD30 pKa = 4.32 SFVCSDD36 pKa = 4.12 DD37 pKa = 3.84 ALEE40 pKa = 3.87 FHH42 pKa = 6.81 IQGFLHH48 pKa = 6.65 IGNVRR53 pKa = 11.84 VTYY56 pKa = 10.44 IEE58 pKa = 4.4 GVDD61 pKa = 3.7 LFQVSLYY68 pKa = 10.63 DD69 pKa = 3.64 EE70 pKa = 4.95 EE71 pKa = 5.2 GNEE74 pKa = 4.24 VKK76 pKa = 10.63 RR77 pKa = 11.84 IDD79 pKa = 5.6 DD80 pKa = 3.88 VYY82 pKa = 11.25 LDD84 pKa = 3.97 NLAEE88 pKa = 4.45 VIDD91 pKa = 4.15 QNVEE95 pKa = 3.7 NYY97 pKa = 10.21 LVEE100 pKa = 5.04 DD101 pKa = 3.93 YY102 pKa = 10.55 NAKK105 pKa = 9.24 VMNYY109 pKa = 9.84 ILGLL113 pKa = 3.77
Molecular weight: 12.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.973
IPC_protein 3.923
Toseland 3.732
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.834
Rodwell 3.757
Grimsley 3.643
Solomon 3.897
Lehninger 3.846
Nozaki 4.024
DTASelect 4.228
Thurlkill 3.783
EMBOSS 3.846
Sillero 4.05
Patrickios 0.947
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.912
Protein with the highest isoelectric point:
>tr|F3PRE3|F3PRE3_9BACE Replicative DNA helicase OS=Bacteroides fluxus YIT 12057 OX=763034 GN=HMPREF9446_01293 PE=3 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.56 GNSHH6 pKa = 6.64 SNARR10 pKa = 11.84 PKK12 pKa = 10.32 RR13 pKa = 11.84 ANKK16 pKa = 9.25 TKK18 pKa = 8.67 TTGKK22 pKa = 10.37 RR23 pKa = 11.84 IGKK26 pKa = 9.06 SKK28 pKa = 10.31 PSKK31 pKa = 10.93 ANNQLNRR38 pKa = 11.84 RR39 pKa = 11.84 VKK41 pKa = 10.41
Molecular weight: 4.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 10.935
IPC_protein 12.442
Toseland 12.632
ProMoST 13.1
Dawson 12.632
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.618
Grimsley 12.676
Solomon 13.115
Lehninger 13.013
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 12.34
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.973
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3920
0
3920
1321397
35
2040
337.1
38.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.267 ± 0.04
1.302 ± 0.015
5.416 ± 0.027
6.6 ± 0.036
4.584 ± 0.026
6.956 ± 0.035
1.913 ± 0.019
6.706 ± 0.038
6.55 ± 0.037
9.212 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.814 ± 0.018
4.964 ± 0.037
3.772 ± 0.022
3.407 ± 0.019
4.844 ± 0.031
5.995 ± 0.034
5.479 ± 0.028
6.523 ± 0.03
1.263 ± 0.015
4.432 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here