Small anellovirus 2
Average proteome isoelectric point is 8.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q537Y2|Q537Y2_9VIRU ORF1 OS=Small anellovirus 2 OX=289367 PE=4 SV=1
MM1 pKa = 7.59 LCAKK5 pKa = 10.44 DD6 pKa = 4.83 YY7 pKa = 7.44 FTQTDD12 pKa = 4.46 YY13 pKa = 10.31 NTEE16 pKa = 4.14 TKK18 pKa = 9.72 NQLWMSMIADD28 pKa = 3.67 SHH30 pKa = 7.28 DD31 pKa = 3.4 IHH33 pKa = 7.2 CKK35 pKa = 8.8 CHH37 pKa = 5.84 TPFAHH42 pKa = 7.16 LLASIFPPGHH52 pKa = 6.35 SDD54 pKa = 4.08 RR55 pKa = 11.84 EE56 pKa = 4.19 LTINQILTRR65 pKa = 11.84 DD66 pKa = 3.91 FKK68 pKa = 10.65 EE69 pKa = 4.0 LRR71 pKa = 11.84 CLSGGGGEE79 pKa = 4.14 KK80 pKa = 10.87 GFGKK84 pKa = 10.63 EE85 pKa = 3.64 EE86 pKa = 4.0 THH88 pKa = 6.11 TSRR91 pKa = 11.84 EE92 pKa = 4.06 RR93 pKa = 11.84 ITEE96 pKa = 3.92 KK97 pKa = 10.57 EE98 pKa = 3.72 NLYY101 pKa = 10.98 VEE103 pKa = 5.23 DD104 pKa = 3.76 EE105 pKa = 4.19 EE106 pKa = 4.6 LRR108 pKa = 11.84 EE109 pKa = 4.22 FIAAAEE115 pKa = 4.23 DD116 pKa = 3.56 AAGTRR121 pKa = 3.96
Molecular weight: 13.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.063
IPC2_protein 5.118
IPC_protein 5.041
Toseland 5.054
ProMoST 5.194
Dawson 5.054
Bjellqvist 5.169
Wikipedia 4.927
Rodwell 4.991
Grimsley 4.991
Solomon 5.054
Lehninger 5.016
Nozaki 5.194
DTASelect 5.321
Thurlkill 5.067
EMBOSS 5.003
Sillero 5.27
Patrickios 3.923
IPC_peptide 5.067
IPC2_peptide 5.283
IPC2.peptide.svr19 5.197
Protein with the highest isoelectric point:
>tr|Q537X9|Q537X9_9VIRU ORF4 OS=Small anellovirus 2 OX=289367 PE=4 SV=1
MM1 pKa = 7.65 SPGALQRR8 pKa = 11.84 KK9 pKa = 6.97 YY10 pKa = 10.89 LPRR13 pKa = 11.84 NAARR17 pKa = 11.84 GKK19 pKa = 9.54 PPAAHH24 pKa = 6.81 PPAAATAARR33 pKa = 11.84 HH34 pKa = 5.41 QIQPPQINILHH45 pKa = 6.21 EE46 pKa = 4.25 RR47 pKa = 11.84 TTKK50 pKa = 10.01 HH51 pKa = 5.34 VKK53 pKa = 8.34 TSHH56 pKa = 6.66 RR57 pKa = 11.84 NVTLTRR63 pKa = 11.84 FAPGFEE69 pKa = 4.26 QEE71 pKa = 4.31 TEE73 pKa = 3.76 RR74 pKa = 11.84 DD75 pKa = 3.04 LAIAFKK81 pKa = 10.85 RR82 pKa = 11.84 PMRR85 pKa = 11.84 TFKK88 pKa = 10.71 EE89 pKa = 4.1 DD90 pKa = 3.27 TPFYY94 pKa = 9.81 PWLPPEE100 pKa = 4.05 PTVNFHH106 pKa = 7.3 LNFKK110 pKa = 10.21 LKK112 pKa = 10.18 PATSQQ117 pKa = 2.99
Molecular weight: 13.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.794
IPC_protein 10.599
Toseland 10.994
ProMoST 10.687
Dawson 11.038
Bjellqvist 10.745
Wikipedia 11.257
Rodwell 11.316
Grimsley 11.067
Solomon 11.213
Lehninger 11.169
Nozaki 10.95
DTASelect 10.745
Thurlkill 10.965
EMBOSS 11.389
Sillero 10.979
Patrickios 11.067
IPC_peptide 11.213
IPC2_peptide 9.458
IPC2.peptide.svr19 8.664
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1088
117
502
217.6
25.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.963 ± 1.219
1.746 ± 0.372
3.585 ± 0.813
4.412 ± 1.394
4.044 ± 0.67
4.136 ± 0.436
2.941 ± 0.624
5.974 ± 0.662
8.824 ± 1.34
8.456 ± 0.873
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.114 ± 0.515
5.331 ± 0.684
6.618 ± 1.106
7.077 ± 0.991
5.974 ± 2.556
6.801 ± 2.399
7.813 ± 0.66
3.125 ± 0.768
1.746 ± 0.568
4.32 ± 1.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here