Cycloclasticus sp. symbiont of Poecilosclerida sp. N

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Cycloclasticus; unclassified Cycloclasticus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1736 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X1QJF7|A0A1X1QJF7_9GAMM Uncharacterized protein OS=Cycloclasticus sp. symbiont of Poecilosclerida sp. N OX=1840472 GN=A6F72_01500 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.09LLKK5 pKa = 9.19ITAIASLLAASGAASAVEE23 pKa = 3.78ISGNVALTTDD33 pKa = 4.0YY34 pKa = 10.66VWRR37 pKa = 11.84GISQTDD43 pKa = 3.9GSPAIQGGFDD53 pKa = 3.04AGFGNGLYY61 pKa = 10.02TGIWASNVDD70 pKa = 4.05FGGDD74 pKa = 3.01EE75 pKa = 4.31SMEE78 pKa = 4.39LDD80 pKa = 4.91VYY82 pKa = 11.21AGWAGEE88 pKa = 4.08FKK90 pKa = 10.92SVGVDD95 pKa = 2.84IGVLHH100 pKa = 5.92YY101 pKa = 10.13AYY103 pKa = 7.2PTSAPDD109 pKa = 3.38TDD111 pKa = 3.92FTEE114 pKa = 5.55AYY116 pKa = 9.88AGLSYY121 pKa = 11.14GPASLTQYY129 pKa = 10.73FGFDD133 pKa = 3.66AGSDD137 pKa = 3.9DD138 pKa = 4.71IGDD141 pKa = 3.65VDD143 pKa = 4.56FGNYY147 pKa = 7.87TDD149 pKa = 5.18LGLDD153 pKa = 3.14LGEE156 pKa = 4.32YY157 pKa = 10.46NGITLAVHH165 pKa = 6.77AGHH168 pKa = 7.08YY169 pKa = 9.25DD170 pKa = 3.45RR171 pKa = 11.84KK172 pKa = 10.59SGSDD176 pKa = 4.56DD177 pKa = 3.26YY178 pKa = 11.54WDD180 pKa = 3.26WRR182 pKa = 11.84LAASTSLFGVDD193 pKa = 3.08WEE195 pKa = 4.57VAYY198 pKa = 11.2SDD200 pKa = 3.8VDD202 pKa = 3.47NANEE206 pKa = 5.11DD207 pKa = 4.03RR208 pKa = 11.84NDD210 pKa = 3.77DD211 pKa = 3.98PNLVLTVSKK220 pKa = 10.98SLL222 pKa = 3.56

Molecular weight: 23.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X1QMH6|A0A1X1QMH6_9GAMM Nitrate reductase catalytic subunit OS=Cycloclasticus sp. symbiont of Poecilosclerida sp. N OX=1840472 GN=A6F72_04800 PE=3 SV=1
MM1 pKa = 7.43ARR3 pKa = 11.84IAGINIPDD11 pKa = 3.76NKK13 pKa = 10.35HH14 pKa = 5.44IVISLTSIYY23 pKa = 10.69GVGPTRR29 pKa = 11.84SKK31 pKa = 10.95AICKK35 pKa = 9.59AAAIDD40 pKa = 4.0TATKK44 pKa = 10.15ICDD47 pKa = 3.51LSEE50 pKa = 4.49DD51 pKa = 3.54QLEE54 pKa = 4.29LVRR57 pKa = 11.84TEE59 pKa = 3.63VGKK62 pKa = 10.69YY63 pKa = 9.88IIEE66 pKa = 4.58GDD68 pKa = 3.49LRR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.4VSMNIKK78 pKa = 10.26RR79 pKa = 11.84LLDD82 pKa = 3.94LGCFRR87 pKa = 11.84GIRR90 pKa = 11.84HH91 pKa = 5.94RR92 pKa = 11.84RR93 pKa = 11.84SLPVRR98 pKa = 11.84GQRR101 pKa = 11.84TKK103 pKa = 10.45TNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.72GPRR113 pKa = 11.84KK114 pKa = 9.36PIRR117 pKa = 11.84KK118 pKa = 9.01

Molecular weight: 13.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1736

0

1736

513302

51

2124

295.7

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.399 ± 0.069

1.089 ± 0.023

5.628 ± 0.04

6.206 ± 0.059

4.068 ± 0.04

7.058 ± 0.057

2.187 ± 0.03

6.71 ± 0.044

6.317 ± 0.066

10.169 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.032

4.38 ± 0.051

3.759 ± 0.039

4.054 ± 0.044

4.672 ± 0.039

6.55 ± 0.05

5.356 ± 0.058

6.911 ± 0.051

1.105 ± 0.021

2.846 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski