Panicum streak virus - Karino

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus; Panicum streak virus

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q84370|Q84370_9GEMI Replication-associated protein OS=Panicum streak virus - Karino OX=268779 GN=RepA PE=3 SV=1
MM1 pKa = 6.97STSLSITSDD10 pKa = 2.59GRR12 pKa = 11.84HH13 pKa = 4.58SVRR16 pKa = 11.84SFRR19 pKa = 11.84HH20 pKa = 5.12RR21 pKa = 11.84NANTFLTYY29 pKa = 10.26SKK31 pKa = 10.88CPLEE35 pKa = 3.93PEE37 pKa = 4.86FIGEE41 pKa = 3.97HH42 pKa = 6.35LFRR45 pKa = 11.84LTKK48 pKa = 10.5DD49 pKa = 3.58FEE51 pKa = 4.03PAYY54 pKa = 9.19ILVVRR59 pKa = 11.84EE60 pKa = 3.81THH62 pKa = 6.89QDD64 pKa = 3.85GTWHH68 pKa = 6.13CHH70 pKa = 6.66ALLQCIKK77 pKa = 10.37PVTTRR82 pKa = 11.84DD83 pKa = 3.08EE84 pKa = 4.6RR85 pKa = 11.84YY86 pKa = 9.54FDD88 pKa = 3.44IDD90 pKa = 3.67RR91 pKa = 11.84YY92 pKa = 10.35HH93 pKa = 7.33PNIQSAKK100 pKa = 8.17STDD103 pKa = 3.09KK104 pKa = 10.55VRR106 pKa = 11.84EE107 pKa = 4.1YY108 pKa = 10.68ILKK111 pKa = 10.31DD112 pKa = 3.71PKK114 pKa = 11.09DD115 pKa = 3.29KK116 pKa = 10.5WEE118 pKa = 4.22KK119 pKa = 8.82GTYY122 pKa = 9.25IPRR125 pKa = 11.84KK126 pKa = 9.51KK127 pKa = 10.78SFVPPGKK134 pKa = 10.11EE135 pKa = 3.6NSEE138 pKa = 4.16KK139 pKa = 10.64KK140 pKa = 9.44PSKK143 pKa = 10.63DD144 pKa = 3.13EE145 pKa = 3.95VMKK148 pKa = 10.81EE149 pKa = 3.71IMTHH153 pKa = 4.37ATSRR157 pKa = 11.84AEE159 pKa = 3.89YY160 pKa = 10.09LSLVQTSLPYY170 pKa = 10.68DD171 pKa = 3.35WATKK175 pKa = 10.42LSYY178 pKa = 10.7FEE180 pKa = 4.77YY181 pKa = 10.06SASRR185 pKa = 11.84LFPDD189 pKa = 3.14IAEE192 pKa = 4.97PYY194 pKa = 9.71SNPHH198 pKa = 6.94PATDD202 pKa = 4.42PDD204 pKa = 4.21LLCNEE209 pKa = 4.77TLQDD213 pKa = 3.25WLEE216 pKa = 3.89PNIYY220 pKa = 9.6QVSPKK225 pKa = 10.59AYY227 pKa = 8.25MLLEE231 pKa = 4.21PSCLSLEE238 pKa = 4.12QAKK241 pKa = 10.7ADD243 pKa = 4.11LEE245 pKa = 4.43WLSEE249 pKa = 4.2TTRR252 pKa = 11.84LFQEE256 pKa = 4.37QEE258 pKa = 4.35SEE260 pKa = 4.12ASTSSAQHH268 pKa = 5.76GQVKK272 pKa = 10.26HH273 pKa = 6.34PGPEE277 pKa = 4.01ASDD280 pKa = 3.42GTTTGRR286 pKa = 11.84ITSTGLHH293 pKa = 5.08TMKK296 pKa = 10.81KK297 pKa = 9.42LRR299 pKa = 11.84TMSWTTFPSSSVRR312 pKa = 11.84AGSSSSAVKK321 pKa = 9.1KK322 pKa = 6.46TTT324 pKa = 3.14

Molecular weight: 36.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q84370|Q84370_9GEMI Replication-associated protein OS=Panicum streak virus - Karino OX=268779 GN=RepA PE=3 SV=1
MM1 pKa = 7.63SGALKK6 pKa = 10.35RR7 pKa = 11.84KK8 pKa = 10.08RR9 pKa = 11.84SDD11 pKa = 2.87EE12 pKa = 4.38VAWSRR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.95PVKK22 pKa = 10.44KK23 pKa = 9.94QDD25 pKa = 3.19TGFPLPRR32 pKa = 11.84AGPSVRR38 pKa = 11.84RR39 pKa = 11.84GLPALQIQTLTAAGDD54 pKa = 3.66TMITVPSGGICSLIGTYY71 pKa = 10.73ARR73 pKa = 11.84GSDD76 pKa = 3.18EE77 pKa = 4.43GNRR80 pKa = 11.84HH81 pKa = 4.63TNEE84 pKa = 3.56TLTYY88 pKa = 10.08KK89 pKa = 10.59VALDD93 pKa = 3.36YY94 pKa = 11.55HH95 pKa = 6.49FVATAAACKK104 pKa = 9.83YY105 pKa = 9.96SSIGVGVMWLVYY117 pKa = 9.96DD118 pKa = 4.42AQPTGNSPEE127 pKa = 4.06VKK129 pKa = 10.2DD130 pKa = 4.13IFPHH134 pKa = 6.71SDD136 pKa = 2.91TLSAFPYY143 pKa = 7.04TWKK146 pKa = 10.5VGRR149 pKa = 11.84EE150 pKa = 3.83VCHH153 pKa = 6.21RR154 pKa = 11.84FVVKK158 pKa = 10.44RR159 pKa = 11.84RR160 pKa = 11.84WCFTMEE166 pKa = 3.97TNGRR170 pKa = 11.84IGSDD174 pKa = 3.35VPPANTAWPPCKK186 pKa = 9.69KK187 pKa = 10.32DD188 pKa = 3.04IYY190 pKa = 10.04FHH192 pKa = 6.77KK193 pKa = 10.19FCTGLGVKK201 pKa = 7.99TEE203 pKa = 4.15WKK205 pKa = 10.26NVTDD209 pKa = 4.33GKK211 pKa = 11.04VGAIKK216 pKa = 10.37KK217 pKa = 8.43GALYY221 pKa = 10.16IVIAPGNGLEE231 pKa = 4.27FTVHH235 pKa = 5.63GQCRR239 pKa = 11.84LYY241 pKa = 10.55FKK243 pKa = 10.94SVGNQQ248 pKa = 2.8

Molecular weight: 27.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

679

107

324

226.3

25.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.775 ± 0.817

2.062 ± 0.382

4.713 ± 0.385

5.449 ± 1.471

3.976 ± 0.517

6.48 ± 2.046

2.651 ± 0.876

3.976 ± 0.223

6.627 ± 1.345

7.658 ± 1.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.62 ± 0.213

2.209 ± 0.676

7.953 ± 2.242

2.798 ± 0.411

5.744 ± 0.345

9.131 ± 1.729

8.1 ± 1.356

6.48 ± 1.866

2.209 ± 0.25

3.387 ± 0.716
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski