Amino acid dipepetide frequency for Polaribacter phage P12002S

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.55AlaAla: 1.55 ± 0.739
0.472AlaCys: 0.472 ± 0.212
3.033AlaAsp: 3.033 ± 0.451
3.302AlaGlu: 3.302 ± 0.416
2.291AlaPhe: 2.291 ± 0.378
1.685AlaGly: 1.685 ± 0.412
0.539AlaHis: 0.539 ± 0.216
3.639AlaIle: 3.639 ± 0.601
5.392AlaLys: 5.392 ± 0.879
4.246AlaLeu: 4.246 ± 0.866
1.28AlaMet: 1.28 ± 0.408
3.168AlaAsn: 3.168 ± 0.445
0.607AlaPro: 0.607 ± 0.167
0.944AlaGln: 0.944 ± 0.269
1.887AlaArg: 1.887 ± 0.413
3.37AlaSer: 3.37 ± 0.552
3.168AlaThr: 3.168 ± 0.686
2.359AlaVal: 2.359 ± 0.458
0.27AlaTrp: 0.27 ± 0.149
1.82AlaTyr: 1.82 ± 0.379
0.0AlaXaa: 0.0 ± 0.0
Cys
0.27CysAla: 0.27 ± 0.152
0.135CysCys: 0.135 ± 0.095
1.011CysAsp: 1.011 ± 0.262
0.674CysGlu: 0.674 ± 0.231
0.472CysPhe: 0.472 ± 0.176
1.146CysGly: 1.146 ± 0.356
0.067CysHis: 0.067 ± 0.069
0.674CysIle: 0.674 ± 0.212
0.876CysLys: 0.876 ± 0.31
0.876CysLeu: 0.876 ± 0.3
0.0CysMet: 0.0 ± 0.0
0.809CysAsn: 0.809 ± 0.197
0.067CysPro: 0.067 ± 0.07
0.135CysGln: 0.135 ± 0.086
0.27CysArg: 0.27 ± 0.143
0.944CysSer: 0.944 ± 0.273
0.674CysThr: 0.674 ± 0.198
0.472CysVal: 0.472 ± 0.175
0.202CysTrp: 0.202 ± 0.107
0.27CysTyr: 0.27 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
3.37AspAla: 3.37 ± 0.82
0.607AspCys: 0.607 ± 0.215
2.898AspAsp: 2.898 ± 0.386
3.909AspGlu: 3.909 ± 0.494
3.437AspPhe: 3.437 ± 0.495
3.707AspGly: 3.707 ± 0.544
0.472AspHis: 0.472 ± 0.182
5.931AspIle: 5.931 ± 0.526
6.874AspLys: 6.874 ± 0.607
6.739AspLeu: 6.739 ± 0.541
1.415AspMet: 1.415 ± 0.299
4.313AspAsn: 4.313 ± 0.569
1.146AspPro: 1.146 ± 0.292
1.146AspGln: 1.146 ± 0.211
1.954AspArg: 1.954 ± 0.336
3.168AspSer: 3.168 ± 0.421
2.831AspThr: 2.831 ± 0.475
3.774AspVal: 3.774 ± 0.462
0.809AspTrp: 0.809 ± 0.293
2.831AspTyr: 2.831 ± 0.414
0.0AspXaa: 0.0 ± 0.0
Glu
2.898GluAla: 2.898 ± 0.505
0.472GluCys: 0.472 ± 0.182
3.168GluAsp: 3.168 ± 0.501
5.661GluGlu: 5.661 ± 0.687
3.841GluPhe: 3.841 ± 0.446
3.235GluGly: 3.235 ± 0.426
0.809GluHis: 0.809 ± 0.281
5.998GluIle: 5.998 ± 0.588
7.548GluLys: 7.548 ± 0.81
9.435GluLeu: 9.435 ± 0.971
1.617GluMet: 1.617 ± 0.295
5.863GluAsn: 5.863 ± 0.655
1.887GluPro: 1.887 ± 0.359
3.505GluGln: 3.505 ± 0.495
3.437GluArg: 3.437 ± 0.426
4.246GluSer: 4.246 ± 0.624
4.111GluThr: 4.111 ± 0.556
4.785GluVal: 4.785 ± 0.536
1.078GluTrp: 1.078 ± 0.241
3.841GluTyr: 3.841 ± 0.531
0.0GluXaa: 0.0 ± 0.0
Phe
1.752PheAla: 1.752 ± 0.379
0.472PheCys: 0.472 ± 0.196
3.437PheAsp: 3.437 ± 0.442
3.639PheGlu: 3.639 ± 0.494
1.415PhePhe: 1.415 ± 0.349
2.763PheGly: 2.763 ± 0.41
0.404PheHis: 0.404 ± 0.172
3.168PheIle: 3.168 ± 0.431
4.515PheLys: 4.515 ± 0.635
3.909PheLeu: 3.909 ± 0.526
1.483PheMet: 1.483 ± 0.368
3.841PheAsn: 3.841 ± 0.468
0.404PhePro: 0.404 ± 0.16
1.011PheGln: 1.011 ± 0.26
1.685PheArg: 1.685 ± 0.321
3.235PheSer: 3.235 ± 0.43
2.965PheThr: 2.965 ± 0.556
2.224PheVal: 2.224 ± 0.425
0.539PheTrp: 0.539 ± 0.221
1.415PheTyr: 1.415 ± 0.311
0.0PheXaa: 0.0 ± 0.0
Gly
2.426GlyAla: 2.426 ± 0.39
0.741GlyCys: 0.741 ± 0.224
2.831GlyAsp: 2.831 ± 0.497
3.437GlyGlu: 3.437 ± 0.486
2.494GlyPhe: 2.494 ± 0.457
3.639GlyGly: 3.639 ± 0.68
0.607GlyHis: 0.607 ± 0.228
4.044GlyIle: 4.044 ± 0.41
5.189GlyLys: 5.189 ± 0.439
5.055GlyLeu: 5.055 ± 0.504
1.011GlyMet: 1.011 ± 0.278
4.381GlyAsn: 4.381 ± 0.775
0.0GlyPro: 0.0 ± 0.0
1.011GlyGln: 1.011 ± 0.22
1.752GlyArg: 1.752 ± 0.308
3.976GlySer: 3.976 ± 0.532
3.37GlyThr: 3.37 ± 0.524
4.381GlyVal: 4.381 ± 0.569
0.809GlyTrp: 0.809 ± 0.214
3.1GlyTyr: 3.1 ± 0.7
0.0GlyXaa: 0.0 ± 0.0
His
0.404HisAla: 0.404 ± 0.152
0.0HisCys: 0.0 ± 0.0
0.607HisAsp: 0.607 ± 0.206
1.146HisGlu: 1.146 ± 0.295
0.539HisPhe: 0.539 ± 0.192
0.674HisGly: 0.674 ± 0.243
0.067HisHis: 0.067 ± 0.07
1.011HisIle: 1.011 ± 0.262
1.752HisLys: 1.752 ± 0.343
0.944HisLeu: 0.944 ± 0.247
0.0HisMet: 0.0 ± 0.0
0.741HisAsn: 0.741 ± 0.231
0.607HisPro: 0.607 ± 0.243
0.472HisGln: 0.472 ± 0.25
0.404HisArg: 0.404 ± 0.153
1.078HisSer: 1.078 ± 0.295
0.674HisThr: 0.674 ± 0.232
0.944HisVal: 0.944 ± 0.257
0.067HisTrp: 0.067 ± 0.069
0.472HisTyr: 0.472 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
3.909IleAla: 3.909 ± 0.608
0.607IleCys: 0.607 ± 0.245
5.998IleAsp: 5.998 ± 0.515
6.335IleGlu: 6.335 ± 0.667
2.561IlePhe: 2.561 ± 0.361
4.111IleGly: 4.111 ± 0.585
1.146IleHis: 1.146 ± 0.274
4.65IleIle: 4.65 ± 0.662
10.379IleLys: 10.379 ± 0.921
6.133IleLeu: 6.133 ± 0.668
0.944IleMet: 0.944 ± 0.251
5.998IleAsn: 5.998 ± 0.792
1.954IlePro: 1.954 ± 0.371
2.157IleGln: 2.157 ± 0.448
2.696IleArg: 2.696 ± 0.402
6.335IleSer: 6.335 ± 0.678
5.324IleThr: 5.324 ± 0.765
3.707IleVal: 3.707 ± 0.445
1.213IleTrp: 1.213 ± 0.285
2.224IleTyr: 2.224 ± 0.419
0.0IleXaa: 0.0 ± 0.0
Lys
5.055LysAla: 5.055 ± 0.767
1.078LysCys: 1.078 ± 0.35
7.279LysAsp: 7.279 ± 1.047
11.794LysGlu: 11.794 ± 1.413
3.437LysPhe: 3.437 ± 0.558
6.2LysGly: 6.2 ± 0.707
1.55LysHis: 1.55 ± 0.372
7.616LysIle: 7.616 ± 0.752
11.929LysLys: 11.929 ± 1.425
7.885LysLeu: 7.885 ± 0.649
2.494LysMet: 2.494 ± 0.471
7.481LysAsn: 7.481 ± 1.074
3.437LysPro: 3.437 ± 0.588
3.909LysGln: 3.909 ± 0.646
3.033LysArg: 3.033 ± 0.423
5.257LysSer: 5.257 ± 0.515
7.481LysThr: 7.481 ± 0.609
5.998LysVal: 5.998 ± 0.588
0.809LysTrp: 0.809 ± 0.251
6.537LysTyr: 6.537 ± 0.873
0.0LysXaa: 0.0 ± 0.0
Leu
4.515LeuAla: 4.515 ± 0.649
0.809LeuCys: 0.809 ± 0.246
5.392LeuAsp: 5.392 ± 0.558
6.739LeuGlu: 6.739 ± 0.706
3.302LeuPhe: 3.302 ± 0.418
5.324LeuGly: 5.324 ± 0.607
1.415LeuHis: 1.415 ± 0.297
7.885LeuIle: 7.885 ± 0.78
10.716LeuLys: 10.716 ± 1.286
5.661LeuLeu: 5.661 ± 0.722
2.089LeuMet: 2.089 ± 0.357
6.2LeuAsn: 6.2 ± 0.712
2.561LeuPro: 2.561 ± 0.341
2.561LeuGln: 2.561 ± 0.313
3.302LeuArg: 3.302 ± 0.495
5.661LeuSer: 5.661 ± 0.685
5.122LeuThr: 5.122 ± 0.657
4.111LeuVal: 4.111 ± 0.477
0.944LeuTrp: 0.944 ± 0.291
3.235LeuTyr: 3.235 ± 0.536
0.0LeuXaa: 0.0 ± 0.0
Met
1.415MetAla: 1.415 ± 0.316
0.135MetCys: 0.135 ± 0.085
0.741MetAsp: 0.741 ± 0.249
1.28MetGlu: 1.28 ± 0.291
0.741MetPhe: 0.741 ± 0.225
1.011MetGly: 1.011 ± 0.389
0.27MetHis: 0.27 ± 0.109
1.752MetIle: 1.752 ± 0.347
3.302MetLys: 3.302 ± 0.505
1.55MetLeu: 1.55 ± 0.368
0.472MetMet: 0.472 ± 0.19
1.617MetAsn: 1.617 ± 0.333
0.472MetPro: 0.472 ± 0.166
1.078MetGln: 1.078 ± 0.292
0.674MetArg: 0.674 ± 0.193
1.213MetSer: 1.213 ± 0.275
1.415MetThr: 1.415 ± 0.295
0.809MetVal: 0.809 ± 0.216
0.135MetTrp: 0.135 ± 0.086
0.741MetTyr: 0.741 ± 0.254
0.0MetXaa: 0.0 ± 0.0
Asn
3.302AsnAla: 3.302 ± 0.374
0.944AsnCys: 0.944 ± 0.292
3.909AsnAsp: 3.909 ± 0.453
5.594AsnGlu: 5.594 ± 0.778
3.639AsnPhe: 3.639 ± 0.555
5.257AsnGly: 5.257 ± 0.841
1.011AsnHis: 1.011 ± 0.31
4.785AsnIle: 4.785 ± 0.712
7.346AsnLys: 7.346 ± 0.859
6.133AsnLeu: 6.133 ± 0.754
1.752AsnMet: 1.752 ± 0.402
4.381AsnAsn: 4.381 ± 0.605
2.157AsnPro: 2.157 ± 0.392
1.82AsnGln: 1.82 ± 0.38
3.302AsnArg: 3.302 ± 0.385
4.313AsnSer: 4.313 ± 0.615
4.178AsnThr: 4.178 ± 0.693
3.572AsnVal: 3.572 ± 0.515
0.809AsnTrp: 0.809 ± 0.26
3.841AsnTyr: 3.841 ± 0.611
0.0AsnXaa: 0.0 ± 0.0
Pro
0.472ProAla: 0.472 ± 0.18
0.0ProCys: 0.0 ± 0.0
1.146ProAsp: 1.146 ± 0.289
1.55ProGlu: 1.55 ± 0.249
1.887ProPhe: 1.887 ± 0.479
0.27ProGly: 0.27 ± 0.113
0.202ProHis: 0.202 ± 0.114
1.752ProIle: 1.752 ± 0.333
2.426ProLys: 2.426 ± 0.437
2.426ProLeu: 2.426 ± 0.364
0.607ProMet: 0.607 ± 0.226
1.82ProAsn: 1.82 ± 0.364
0.404ProPro: 0.404 ± 0.167
0.876ProGln: 0.876 ± 0.259
0.741ProArg: 0.741 ± 0.215
1.078ProSer: 1.078 ± 0.3
2.494ProThr: 2.494 ± 0.461
0.539ProVal: 0.539 ± 0.213
0.067ProTrp: 0.067 ± 0.064
1.415ProTyr: 1.415 ± 0.389
0.0ProXaa: 0.0 ± 0.0
Gln
2.157GlnAla: 2.157 ± 0.466
0.27GlnCys: 0.27 ± 0.137
1.617GlnAsp: 1.617 ± 0.278
2.696GlnGlu: 2.696 ± 0.405
1.146GlnPhe: 1.146 ± 0.21
1.617GlnGly: 1.617 ± 0.373
0.607GlnHis: 0.607 ± 0.197
2.561GlnIle: 2.561 ± 0.323
3.505GlnLys: 3.505 ± 0.553
2.022GlnLeu: 2.022 ± 0.345
0.944GlnMet: 0.944 ± 0.365
2.022GlnAsn: 2.022 ± 0.374
0.809GlnPro: 0.809 ± 0.239
1.011GlnGln: 1.011 ± 0.358
1.752GlnArg: 1.752 ± 0.316
1.685GlnSer: 1.685 ± 0.36
1.483GlnThr: 1.483 ± 0.282
2.224GlnVal: 2.224 ± 0.383
0.202GlnTrp: 0.202 ± 0.113
1.146GlnTyr: 1.146 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
1.82ArgAla: 1.82 ± 0.414
0.27ArgCys: 0.27 ± 0.116
2.022ArgAsp: 2.022 ± 0.331
1.954ArgGlu: 1.954 ± 0.369
1.55ArgPhe: 1.55 ± 0.408
2.157ArgGly: 2.157 ± 0.458
0.337ArgHis: 0.337 ± 0.161
3.37ArgIle: 3.37 ± 0.525
3.639ArgLys: 3.639 ± 0.657
4.313ArgLeu: 4.313 ± 0.449
0.944ArgMet: 0.944 ± 0.296
1.887ArgAsn: 1.887 ± 0.414
0.741ArgPro: 0.741 ± 0.197
1.415ArgGln: 1.415 ± 0.329
1.28ArgArg: 1.28 ± 0.258
1.954ArgSer: 1.954 ± 0.373
2.022ArgThr: 2.022 ± 0.325
2.157ArgVal: 2.157 ± 0.386
0.337ArgTrp: 0.337 ± 0.141
2.224ArgTyr: 2.224 ± 0.497
0.0ArgXaa: 0.0 ± 0.0
Ser
2.426SerAla: 2.426 ± 0.456
0.741SerCys: 0.741 ± 0.233
3.639SerAsp: 3.639 ± 0.539
5.189SerGlu: 5.189 ± 0.543
3.437SerPhe: 3.437 ± 0.375
2.831SerGly: 2.831 ± 0.487
0.944SerHis: 0.944 ± 0.302
4.987SerIle: 4.987 ± 0.543
6.807SerLys: 6.807 ± 0.85
5.189SerLeu: 5.189 ± 0.662
1.146SerMet: 1.146 ± 0.377
4.987SerAsn: 4.987 ± 0.663
0.944SerPro: 0.944 ± 0.206
1.82SerGln: 1.82 ± 0.301
2.089SerArg: 2.089 ± 0.462
4.044SerSer: 4.044 ± 0.556
3.841SerThr: 3.841 ± 0.71
3.639SerVal: 3.639 ± 0.566
0.741SerTrp: 0.741 ± 0.244
2.898SerTyr: 2.898 ± 0.504
0.0SerXaa: 0.0 ± 0.0
Thr
3.168ThrAla: 3.168 ± 0.574
0.876ThrCys: 0.876 ± 0.279
4.448ThrAsp: 4.448 ± 0.653
4.381ThrGlu: 4.381 ± 0.572
2.628ThrPhe: 2.628 ± 0.374
2.965ThrGly: 2.965 ± 0.674
0.876ThrHis: 0.876 ± 0.245
4.987ThrIle: 4.987 ± 0.689
6.47ThrLys: 6.47 ± 0.819
4.852ThrLeu: 4.852 ± 0.535
1.213ThrMet: 1.213 ± 0.373
4.515ThrAsn: 4.515 ± 0.731
1.617ThrPro: 1.617 ± 0.433
2.763ThrGln: 2.763 ± 0.532
2.022ThrArg: 2.022 ± 0.375
3.437ThrSer: 3.437 ± 0.559
4.515ThrThr: 4.515 ± 0.612
3.639ThrVal: 3.639 ± 0.568
0.404ThrTrp: 0.404 ± 0.151
2.359ThrTyr: 2.359 ± 0.384
0.0ThrXaa: 0.0 ± 0.0
Val
1.954ValAla: 1.954 ± 0.331
0.809ValCys: 0.809 ± 0.233
4.044ValAsp: 4.044 ± 0.632
3.572ValGlu: 3.572 ± 0.582
2.426ValPhe: 2.426 ± 0.367
2.898ValGly: 2.898 ± 0.443
0.674ValHis: 0.674 ± 0.227
4.65ValIle: 4.65 ± 0.449
6.605ValLys: 6.605 ± 0.646
4.718ValLeu: 4.718 ± 0.568
0.337ValMet: 0.337 ± 0.155
3.639ValAsn: 3.639 ± 0.642
1.348ValPro: 1.348 ± 0.276
1.55ValGln: 1.55 ± 0.384
2.022ValArg: 2.022 ± 0.458
4.92ValSer: 4.92 ± 0.619
3.1ValThr: 3.1 ± 0.557
4.178ValVal: 4.178 ± 0.557
0.607ValTrp: 0.607 ± 0.165
1.887ValTyr: 1.887 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
0.876TrpAla: 0.876 ± 0.32
0.0TrpCys: 0.0 ± 0.0
1.213TrpAsp: 1.213 ± 0.321
0.404TrpGlu: 0.404 ± 0.165
0.674TrpPhe: 0.674 ± 0.197
0.337TrpGly: 0.337 ± 0.16
0.0TrpHis: 0.0 ± 0.0
1.28TrpIle: 1.28 ± 0.283
0.472TrpLys: 0.472 ± 0.153
1.213TrpLeu: 1.213 ± 0.265
0.135TrpMet: 0.135 ± 0.11
1.213TrpAsn: 1.213 ± 0.284
0.0TrpPro: 0.0 ± 0.0
0.404TrpGln: 0.404 ± 0.172
0.472TrpArg: 0.472 ± 0.169
0.539TrpSer: 0.539 ± 0.232
0.809TrpThr: 0.809 ± 0.225
0.674TrpVal: 0.674 ± 0.237
0.0TrpTrp: 0.0 ± 0.0
0.135TrpTyr: 0.135 ± 0.083
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.28TyrAla: 1.28 ± 0.245
0.607TyrCys: 0.607 ± 0.261
2.898TyrAsp: 2.898 ± 0.45
4.178TyrGlu: 4.178 ± 0.546
2.359TyrPhe: 2.359 ± 0.43
2.426TyrGly: 2.426 ± 0.425
0.607TyrHis: 0.607 ± 0.203
3.639TyrIle: 3.639 ± 0.483
4.515TyrLys: 4.515 ± 0.561
4.246TyrLeu: 4.246 ± 0.51
0.876TyrMet: 0.876 ± 0.273
3.168TyrAsn: 3.168 ± 0.524
1.011TyrPro: 1.011 ± 0.256
1.887TyrGln: 1.887 ± 0.342
1.617TyrArg: 1.617 ± 0.339
1.887TyrSer: 1.887 ± 0.347
2.561TyrThr: 2.561 ± 0.512
1.752TyrVal: 1.752 ± 0.378
0.809TyrTrp: 0.809 ± 0.255
2.359TyrTyr: 2.359 ± 0.466
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (14839 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski