Streptomyces phage Joe

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Camvirus; unclassified Camvirus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0GP15|A0A1J0GP15_9CAUD Uncharacterized protein OS=Streptomyces phage Joe OX=1913034 GN=Joe_10 PE=4 SV=1
MM1 pKa = 7.41SNINIADD8 pKa = 5.14AIATIAAAFEE18 pKa = 4.35VTEE21 pKa = 4.25VPDD24 pKa = 3.69EE25 pKa = 4.03VRR27 pKa = 11.84EE28 pKa = 4.06ALEE31 pKa = 3.89TLVEE35 pKa = 4.08VEE37 pKa = 5.52RR38 pKa = 11.84DD39 pKa = 3.15DD40 pKa = 5.69AYY42 pKa = 11.44QDD44 pKa = 3.6GHH46 pKa = 7.48SDD48 pKa = 3.93GYY50 pKa = 11.33SEE52 pKa = 5.93GEE54 pKa = 3.76DD55 pKa = 3.08TAYY58 pKa = 10.93SEE60 pKa = 5.68GYY62 pKa = 10.5DD63 pKa = 3.33EE64 pKa = 5.51GRR66 pKa = 11.84SEE68 pKa = 5.51GYY70 pKa = 10.49DD71 pKa = 3.18EE72 pKa = 4.69GHH74 pKa = 6.27TDD76 pKa = 3.66GYY78 pKa = 11.52DD79 pKa = 2.96EE80 pKa = 4.9GFEE83 pKa = 4.23DD84 pKa = 5.01GKK86 pKa = 11.26AEE88 pKa = 5.31AEE90 pKa = 4.46DD91 pKa = 4.63DD92 pKa = 4.01DD93 pKa = 4.78AAA95 pKa = 6.02

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0GNW2|A0A1J0GNW2_9CAUD Uncharacterized protein OS=Streptomyces phage Joe OX=1913034 GN=Joe_21 PE=4 SV=1
MM1 pKa = 7.36SLQRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84GQVARR12 pKa = 11.84IWKK15 pKa = 8.72TSVEE19 pKa = 3.76TDD21 pKa = 2.82RR22 pKa = 11.84RR23 pKa = 11.84GNEE26 pKa = 3.94VVRR29 pKa = 11.84ASADD33 pKa = 3.35GPHH36 pKa = 6.38EE37 pKa = 4.04VRR39 pKa = 11.84AAFIPQRR46 pKa = 11.84SAKK49 pKa = 10.65AEE51 pKa = 4.01VPGQQLINVTRR62 pKa = 11.84MIVAADD68 pKa = 4.06LEE70 pKa = 5.08DD71 pKa = 3.4VTLWSRR77 pKa = 11.84VEE79 pKa = 3.96YY80 pKa = 9.82MGKK83 pKa = 8.98QWDD86 pKa = 4.01IVTPPAYY93 pKa = 9.95HH94 pKa = 6.36HH95 pKa = 6.74GPRR98 pKa = 11.84KK99 pKa = 9.09SRR101 pKa = 11.84HH102 pKa = 3.61WSIDD106 pKa = 2.69IRR108 pKa = 11.84EE109 pKa = 4.17RR110 pKa = 11.84TT111 pKa = 3.47

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

15011

38

1464

185.3

20.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.692 ± 0.44

0.806 ± 0.151

6.382 ± 0.317

6.635 ± 0.433

3.098 ± 0.188

8.261 ± 0.329

1.859 ± 0.211

4.237 ± 0.354

4.623 ± 0.383

8.381 ± 0.463

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.198 ± 0.138

3.031 ± 0.166

5.19 ± 0.299

3.138 ± 0.178

6.402 ± 0.449

6.189 ± 0.339

6.808 ± 0.309

7.328 ± 0.269

1.939 ± 0.137

2.805 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski