Mycobacterium phage Ebony

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5H0T8|A0A2Z5H0T8_9CAUD Uncharacterized protein OS=Mycobacterium phage Ebony OX=2234074 GN=19 PE=4 SV=1
MM1 pKa = 7.18ITFEE5 pKa = 3.97QARR8 pKa = 11.84QIVFDD13 pKa = 4.28NLAGQYY19 pKa = 9.84PPEE22 pKa = 5.96ADD24 pKa = 3.45FQVATWGWEE33 pKa = 3.85NDD35 pKa = 3.46DD36 pKa = 5.35LYY38 pKa = 11.58QLVAGPYY45 pKa = 9.04AMVYY49 pKa = 9.75VSRR52 pKa = 11.84GPQDD56 pKa = 5.02DD57 pKa = 3.8EE58 pKa = 4.05WLEE61 pKa = 4.75DD62 pKa = 3.62GDD64 pKa = 5.06GPFIMVNKK72 pKa = 8.13EE73 pKa = 3.55TGEE76 pKa = 4.06YY77 pKa = 9.09IEE79 pKa = 5.37EE80 pKa = 4.2YY81 pKa = 10.67GEE83 pKa = 4.28PFLIPDD89 pKa = 3.73ARR91 pKa = 11.84TIGEE95 pKa = 4.19PQPAPP100 pKa = 3.39

Molecular weight: 11.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5H102|A0A2Z5H102_9CAUD Uncharacterized protein OS=Mycobacterium phage Ebony OX=2234074 GN=98 PE=4 SV=1
MM1 pKa = 7.71PEE3 pKa = 3.87DD4 pKa = 3.22WEE6 pKa = 4.43LNYY9 pKa = 10.51RR10 pKa = 11.84LPVLSAARR18 pKa = 11.84WMCQVKK24 pKa = 10.51GPGCVGVASEE34 pKa = 4.24VDD36 pKa = 3.59HH37 pKa = 6.9IKK39 pKa = 10.61RR40 pKa = 11.84GNDD43 pKa = 3.11HH44 pKa = 6.61SRR46 pKa = 11.84SNLQAICRR54 pKa = 11.84VCHH57 pKa = 5.79GKK59 pKa = 10.33KK60 pKa = 9.87SAAEE64 pKa = 3.95GVARR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84EE71 pKa = 3.69LRR73 pKa = 11.84ARR75 pKa = 11.84RR76 pKa = 11.84KK77 pKa = 9.79RR78 pKa = 11.84PPEE81 pKa = 3.7RR82 pKa = 11.84HH83 pKa = 6.36PGRR86 pKa = 11.84LL87 pKa = 3.28

Molecular weight: 9.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

16140

25

842

164.7

18.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.703 ± 0.346

0.936 ± 0.111

6.437 ± 0.192

7.162 ± 0.245

3.371 ± 0.171

8.16 ± 0.478

2.119 ± 0.173

5.105 ± 0.158

4.554 ± 0.262

8.055 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.336 ± 0.11

3.389 ± 0.152

5.483 ± 0.217

3.569 ± 0.188

6.394 ± 0.346

5.229 ± 0.216

5.675 ± 0.247

7.379 ± 0.238

1.989 ± 0.141

2.955 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski