Arthrobacter phage KBurrousTX
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 107 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386KBC4|A0A386KBC4_9CAUD RecE-like exonuclease OS=Arthrobacter phage KBurrousTX OX=2315608 GN=46 PE=4 SV=1
MM1 pKa = 6.7 TANVQEE7 pKa = 5.6 ALYY10 pKa = 9.81 AWCADD15 pKa = 3.1 CSMGSEE21 pKa = 4.1 VGILRR26 pKa = 11.84 AEE28 pKa = 4.09 AEE30 pKa = 4.06 AWADD34 pKa = 3.24 EE35 pKa = 4.26 HH36 pKa = 7.48 DD37 pKa = 4.3 ALNHH41 pKa = 5.58 VEE43 pKa = 4.35 EE44 pKa = 4.67 PP45 pKa = 3.65
Molecular weight: 4.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.853
IPC2_protein 4.062
IPC_protein 3.872
Toseland 3.732
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.732
Rodwell 3.719
Grimsley 3.656
Solomon 3.808
Lehninger 3.757
Nozaki 3.973
DTASelect 4.05
Thurlkill 3.77
EMBOSS 3.757
Sillero 3.986
Patrickios 0.604
IPC_peptide 3.808
IPC2_peptide 3.973
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|A0A386K879|A0A386K879_9CAUD Uncharacterized protein OS=Arthrobacter phage KBurrousTX OX=2315608 GN=54 PE=4 SV=1
MM1 pKa = 7.62 PIKK4 pKa = 10.4 RR5 pKa = 11.84 GANGRR10 pKa = 11.84 FVANGGRR17 pKa = 11.84 VRR19 pKa = 11.84 SKK21 pKa = 10.86 GKK23 pKa = 10.44 SGGKK27 pKa = 9.21 AASGGAALAKK37 pKa = 10.18 RR38 pKa = 11.84 GVDD41 pKa = 3.67 HH42 pKa = 7.35 ILARR46 pKa = 11.84 NAAAAKK52 pKa = 9.93 AKK54 pKa = 10.51 KK55 pKa = 8.27 PTRR58 pKa = 11.84 KK59 pKa = 8.36 QQRR62 pKa = 11.84 ADD64 pKa = 3.29 AMKK67 pKa = 10.22 KK68 pKa = 9.91 AQSKK72 pKa = 8.92 MLAAANAKK80 pKa = 9.89 NKK82 pKa = 10.07 RR83 pKa = 3.62
Molecular weight: 8.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.287
IPC2_protein 10.643
IPC_protein 12.149
Toseland 12.34
ProMoST 12.808
Dawson 12.34
Bjellqvist 12.31
Wikipedia 12.793
Rodwell 12.325
Grimsley 12.384
Solomon 12.822
Lehninger 12.72
Nozaki 12.34
DTASelect 12.31
Thurlkill 12.34
EMBOSS 12.822
Sillero 12.34
Patrickios 12.047
IPC_peptide 12.822
IPC2_peptide 11.798
IPC2.peptide.svr19 8.997
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
107
0
107
22523
42
1258
210.5
22.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.117 ± 0.404
0.839 ± 0.102
6.06 ± 0.164
6.22 ± 0.406
2.913 ± 0.206
8.267 ± 0.33
2.229 ± 0.205
4.151 ± 0.232
4.227 ± 0.259
7.597 ± 0.202
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.322 ± 0.114
3.73 ± 0.15
6.007 ± 0.272
3.419 ± 0.137
5.883 ± 0.298
4.853 ± 0.242
7.259 ± 0.337
7.588 ± 0.234
1.834 ± 0.164
2.486 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here