Salvia splendens (Scarlet sage)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Mentheae; Salviinae; Salvia;

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D9A735|A0A4D9A735_SALSN Uncharacterized protein OS=Salvia splendens OX=180675 GN=Saspl_037418 PE=4 SV=1
MM1 pKa = 7.76ALNNNLNLLFILLFFSSLLLPNHH24 pKa = 7.11LAQSPRR30 pKa = 11.84PAPGSSQGPAPGPSLYY46 pKa = 10.56DD47 pKa = 4.04DD48 pKa = 5.55EE49 pKa = 4.76PAPWPSLFDD58 pKa = 4.69DD59 pKa = 5.02EE60 pKa = 4.87PAPGPSLFDD69 pKa = 5.51DD70 pKa = 3.84IDD72 pKa = 3.97YY73 pKa = 11.27QIDD76 pKa = 3.73TQNPSISLPPTSSEE90 pKa = 4.23APSIAPSNEE99 pKa = 3.45YY100 pKa = 10.66DD101 pKa = 4.01LSPSNAPSDD110 pKa = 5.06DD111 pKa = 4.26DD112 pKa = 4.25PADD115 pKa = 3.78PALEE119 pKa = 4.6KK120 pKa = 10.38ICEE123 pKa = 4.48STDD126 pKa = 3.29HH127 pKa = 7.15PALCLSTVAPYY138 pKa = 11.03LDD140 pKa = 4.61GEE142 pKa = 4.39TDD144 pKa = 3.17IQSVLDD150 pKa = 3.57VAIQAGAVFSRR161 pKa = 11.84YY162 pKa = 8.64GQEE165 pKa = 4.12TAQKK169 pKa = 10.88LAMNPGNPPQHH180 pKa = 6.65SSVLSDD186 pKa = 3.51CRR188 pKa = 11.84DD189 pKa = 3.52GFEE192 pKa = 4.36TAAEE196 pKa = 4.32NYY198 pKa = 9.6GKK200 pKa = 10.46AADD203 pKa = 4.21ALAAQDD209 pKa = 3.6KK210 pKa = 9.44STVNSMLSAVITYY223 pKa = 10.12IGDD226 pKa = 3.83CQDD229 pKa = 4.08TISTDD234 pKa = 3.51SPLHH238 pKa = 5.93SLTDD242 pKa = 3.41KK243 pKa = 10.82LINMTSNCLAISSIINN259 pKa = 3.24

Molecular weight: 27.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D9AFZ0|A0A4D9AFZ0_SALSN Protein kinase domain-containing protein OS=Salvia splendens OX=180675 GN=Saspl_019786 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84CKK4 pKa = 10.3KK5 pKa = 9.86HH6 pKa = 6.67AADD9 pKa = 4.03LSSGVGVCASCLRR22 pKa = 11.84DD23 pKa = 3.22RR24 pKa = 11.84LFAIMEE30 pKa = 4.21AQALKK35 pKa = 10.46QSQQQQQQQNPPPLSFPRR53 pKa = 11.84SVSPYY58 pKa = 8.41ISRR61 pKa = 11.84RR62 pKa = 11.84PTDD65 pKa = 3.04TAAATWQIHH74 pKa = 4.82GYY76 pKa = 9.01EE77 pKa = 4.08RR78 pKa = 11.84SLYY81 pKa = 7.26TTPQLAPAAAGEE93 pKa = 4.04ISHH96 pKa = 6.61GKK98 pKa = 7.02GHH100 pKa = 6.76RR101 pKa = 11.84GGRR104 pKa = 11.84FSSLFSGLFRR114 pKa = 11.84SKK116 pKa = 10.84SDD118 pKa = 3.02KK119 pKa = 10.86HH120 pKa = 6.56DD121 pKa = 4.19FNSSSDD127 pKa = 3.44QGVSGQSCSASPFRR141 pKa = 11.84FSSMIPGRR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84KK152 pKa = 9.6QIRR155 pKa = 11.84SFSVDD160 pKa = 2.99EE161 pKa = 4.27SSIGAQQSSCRR172 pKa = 11.84NRR174 pKa = 11.84DD175 pKa = 3.04RR176 pKa = 11.84GMSPARR182 pKa = 11.84CSDD185 pKa = 3.5EE186 pKa = 5.36EE187 pKa = 4.21EE188 pKa = 5.47DD189 pKa = 3.91EE190 pKa = 4.52HH191 pKa = 8.43CHH193 pKa = 6.08GGSSGYY199 pKa = 10.36SSEE202 pKa = 4.99SSQGWRR208 pKa = 11.84QTPRR212 pKa = 11.84RR213 pKa = 11.84TPARR217 pKa = 11.84SRR219 pKa = 11.84RR220 pKa = 11.84RR221 pKa = 11.84GGGGCQNRR229 pKa = 11.84NLSGLAFCLSPLVRR243 pKa = 11.84ASPSRR248 pKa = 11.84QWNQKK253 pKa = 9.37GIPPEE258 pKa = 3.88AVLPGEE264 pKa = 4.31TRR266 pKa = 11.84VPAKK270 pKa = 9.99PHH272 pKa = 6.02LSAAASFCKK281 pKa = 10.16NRR283 pKa = 11.84SRR285 pKa = 11.84KK286 pKa = 9.38LADD289 pKa = 3.75FGRR292 pKa = 11.84YY293 pKa = 7.44NSRR296 pKa = 11.84HH297 pKa = 4.49

Molecular weight: 32.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52672

0

52672

22732906

50

5585

431.6

48.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.264 ± 0.01

1.859 ± 0.005

5.436 ± 0.007

6.534 ± 0.012

4.101 ± 0.007

6.613 ± 0.01

2.391 ± 0.005

5.173 ± 0.007

5.886 ± 0.01

9.692 ± 0.012

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.489 ± 0.004

4.249 ± 0.007

4.91 ± 0.01

3.537 ± 0.007

5.523 ± 0.007

8.878 ± 0.013

4.742 ± 0.007

6.672 ± 0.008

1.27 ± 0.004

2.781 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski